LOCUS       sequence-354463-       14665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the rat TrkC fused in the N-terminus with mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14665)
  TITLE     Neurotrophin related plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLZRS-mVenus-TrkC
FEATURES             Location/Qualifiers
     source          1..14665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(2407..3117,3118..3120,3121..3123))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2449..2470)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2798..2817
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3057..3078
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    3269..3642
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(3315..3331)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(3652..4068)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(3668..3684)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3700..3722)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    4133..4490
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             4555..5024
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5561..5665
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5666..5734,5735..6526)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5884..5903)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7020..8809
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(9111..11036)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     promoter        11465..11964
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11500..11523)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11897..11916
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             12047..12646
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12047..12066)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12543..12563
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    12876..13010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12913..12932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12967..12986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13133..13720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13621..13640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13874..13891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             14021..14490
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 tgctggcggc ccctcgagct agccaagaat gtccaggtag attggggtgg ccttccccaa
       61 agcatggagg attttgtaaa tctccttaat attcagtcgc tgttgtggtt ccctctgcca
      121 gcaccccagc atgacatcat atacttcttt tgggcagact cggggtctct ccaagacacg
      181 gccttgggtg atgcattcaa tgacctctgt gttagagagc tggaaccatg gttgcttccc
      241 ataggtaaag atctcccaaa gaataacccc aaagctccac acatcactct ctgtggtaaa
      301 cttccggtac atgatgcttt caggtggcat ccagcggatg gggagcatgg tgtgtcctcc
      361 caccctgtag taatcagtac tgtagacgtc cctggacatg ccaaaatctc caatcttcac
      421 tagaaggttg gctccaacta ggcagttcct ggtggccagg tcccggtgca caaagtgctg
      481 ggaggccaag tacaccatgc cagaggctat ctgactggcg atgtggagca tctgggagag
      541 tcctagctcc cccttggcct ggcgtggctg tccatccaca aggatcatcg catctggccc
      601 atgggccctg aggaacttgt tcaggtctcc atgcttcatg tattcaaaga ccatgatgag
      661 tggatcccca tcaccacaga ccccatagaa tttgacaata tgctcatgct gcaggttagt
      721 gagcagctcg gcctccctct ggaaatcctt cctggcagcc aaggtgggat ccttcagggc
      781 cttcactgcc acaagcatct tgtctttggt ggggcttaga ttgtagcact cagccaggaa
      841 gacctttcca aaggctccct cacccagttc tctcttcaac acgatgtctc tcctcttgat
      901 gtgttgaaca tatgtgtctg gcttgtggca attgtgtccc tgacggaaat attgggggtt
      961 ctcaatgact ggaattcgag tcatgccaat gaccactgta tccggcccag catccagcga
     1021 tgaaggtgta gtgatgccat ggttgatatg gtgcagtggg ctggctgagt cctcctcccc
     1081 actgataaca gccacaggac ccttcattcc aaatttggac cgtcgaccgt acttgttgat
     1141 catgataaag aggaccacca gaaggacgca ggcaaaagca gcaagtccaa ctgctatgga
     1201 caccccaaaa gtgtcttcct ctggtttgtg ggtcacagtg ataggaggtg tagggctcgc
     1261 atcagactca aagtcaaaga aatctgtgct ctctggaaag ggctccttaa ggaagtggcc
     1321 gttgatggtc tggttggctg tgcccagggc attcttagca atgagggtgt agttgccatt
     1381 gttgtagtgg gtgggcttgt tgaagagcag gcagccctct gagacttcac cctcctggta
     1441 gtagtccata tgaatgatct tggattccct cagtggctgt ccattgtaca gccaatgcag
     1501 cgtgggtgtc gggttgccac gcaccacaaa ctcaatgcaa tgttccaggc gtacctcagg
     1561 ctccaccaag ctcaccacac gtggagggta gtagacagtg agagcaacgc tggcattgct
     1621 catgcccacc acgttctctg caatgcacgt cagggtgaaa ccattgtctt cgctcgtcac
     1681 attcaccagg gtcaagttga tggcatgtac attggtccag ttcagattgg tctgatgagt
     1741 attgatggac tgcagtccgg tgactatcca gtccacatca ggcaaaggag agccagagcc
     1801 attacaagtg attacagcat tgtctccttc tcggacagtc aggttcacgt gactcacact
     1861 aatctcaggg agatcacact gactgatgtt catgcggaag agggggagtt gggagccatc
     1921 agcactgatg cagtagaggc tctggctgtc cagccgtgcc tccccctgtt cctgccacag
     1981 ctgcatccag cggatgtcac agctgcagtt gaagaagttc tgctccagtc tcaattcccg
     2041 aagactcagc gtctggaaga gctgccagga gagtgtggtg agccggttac ttgacaagtt
     2101 tatataacgc aagtggggat tcttagcaaa ggctctgggc tgaatattcc ggagtccaga
     2161 attcttgatg gtcagcttct ggagtcccgt gtagagctcc atatccacag cattgagtgt
     2221 gtgcaggccc cgccagttct ctatgtgtat ggaagtgata ttccttgaga tgtccgtgat
     2281 gttgatgctg gcgttcccat tgctgttccc tgaatcctgc ccttccagga ggggaaagag
     2341 gttcccatcg tccggccgcc ggcaattgat ctcagtcttg ctgcagacac aatttgcagg
     2401 gcaagccttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
     2461 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agcttggact ggtagctcag
     2521 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
     2581 gtcggcgagc tgcacgccgc cgtcctcgat gttgtggcgg atcttgaagt tggccttgat
     2641 gccgttcttc tgcttgtcgg cggtgatata gacgttgtgg ctgttgtagt tgtactccag
     2701 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
     2761 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
     2821 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
     2881 ctgcttcatg tggtcggggt agcgggcgaa gcactgcagg ccgtagccca gggtggtcac
     2941 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atcagcttca gggtcagctt
     3001 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc
     3061 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
     3121 catcagcacg gagcccacat agtccagcca gacgcttccc agcaagaaaa tccgccagaa
     3181 actacacttg gctgggcaaa gagagacatc catggtgcaa gctttctaga gcggccgcga
     3241 attcccgtac caccacactg ggatccggca gtctagagga tggtccaccc ccggggtcgg
     3301 cagccttcac gtgggcggcg tgtatccaag ctgcgatgcc gtctactttg agggcggtgg
     3361 gggtggtcag caggactgtg taaggtcctt tccagcgagg ttctaggttc ttagtctggt
     3421 gtcggcggac ccacactgtg tcgccgactc ggtaagggtg aggtaccacc ggtcggtcca
     3481 gttgttcttg gtaggctgcc gccagaggtc tccagacttc gtgctggact aagtagagag
     3541 cctgtaagtg agcttggaga gaggggctgt tagtaactct tgtcatgtca gggtcaggga
     3601 agtttacaag gggcgggggt gccccatata aatctcatat ggccatatgg gggcgcctag
     3661 agaaggagtg agggctggat aaagggagga tcgaggcggg gtcgaacgag gaggttcaag
     3721 ggggagagac ggggcggatg gaggaagagg aggcggaggc ttagggtgta caaagggctt
     3781 gacccaggga ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg ggacctggtc
     3841 tgggtgtcca tgcgggccag gtgaaaagac cttgatctta acctgggtga tgaggtctcg
     3901 gttaaaggtg ccgtctcgcg gccatccgac gttaaaggtt ggccattctg cagagcagaa
     3961 ggtaacccaa cgtctcttct tgacatctac cgactggttg tgagcgatcc gctcgacatc
     4021 tttccagtga cctaaggtca aacttaaggg agtggtaaca gtctggcccg ggcccatatt
     4081 ttcagacaaa tacagaaaca cagtcagaca gagacaacac agaacgatgc tgcagcagac
     4141 aagacgcgcg gcgcggcttc ggtcccaaac cgaaagcaaa aattcagacg gaggcgggaa
     4201 ctgttttagg ttctcgtctc ctaccagaac cacatatccc tcctctaagg ggggtgcacc
     4261 aaagagtcca aaacgatcgg gatttttgga ctcaggtcgg gccacaaaaa cggcccccga
     4321 agtccctggg acgtctccca gggttgcggc cgggtgttcc gaactcgtca gttccaccac
     4381 gggtccgcca gatacagagc tagttagcta actagtaccg acgcaggcgc ataaaatcag
     4441 tcatagacac tagacaatcg gacagacaca gataagttgc tggccagctt acctcccggt
     4501 ggtgggtcgg tggtccctgg gcaggggtct cccaatcccg gacgagcccc caaatgaaag
     4561 acccccgctg acgggtagtc aatcactcag aggagaccct cccaaggaac agcgagacca
     4621 caagtcggat gcaactgcaa gagggtttat tggatacacg ggtacccggg cgactcagtc
     4681 aatcggagga ctggcgcgcc gagtgagggg ttgtgggctc ttttattgag ctcggggagc
     4741 agaagcgcgc gaacagaagc gagaagcgaa ctgattggtt agttcaaata aggcacaggg
     4801 tcatttcagg tccttggggc accctggaaa catctgatgg ttctctagaa actgctgagg
     4861 gctggaccgc atctggggac catctgttct tggccctgag ccggggcagg aactgcttac
     4921 cacagatatc ctgtttggcc catattcagc tgttccatct gttcttggcc ctgagccggg
     4981 gcaggaactg cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttcc
     5041 tgaccttgat ctgaactttt ctattctcag ttatgtattt ttccatgcct tgcaaaatgg
     5101 cgttacttaa gctagcttgc caaacctaca ggtggggtct ttcacatgta tatgtcaaaa
     5161 ataaaaatca actaattgac tagtaattaa tatgactggc ataatgggaa attgatcctg
     5221 acagatgcaa actggcttct cagcagcgca tttatgttgt caactgagga aggaaacgtt
     5281 aatgacagaa actctaagta atttccacgt ttatctattt ttatttatac tagctttggt
     5341 aacaggaata ttgcagcatt catgcacatt gaaaccctta tgaaataaaa acatctgtgc
     5401 atttaaaatg gaattaacat tttaaatgtt aaaaaaagct ggcttagctt ccccccgccc
     5461 cctagggcat agaacaagtc aaatgcttta tatatttgag tttgggatgt attaggaaac
     5521 tcctaagagc aaagctggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5581 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5641 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5701 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5761 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5821 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5881 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5941 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6001 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6061 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6121 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6181 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6241 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6301 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6361 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6421 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6481 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6541 ttactcatat atactttaga ttgatttgcg gccagatccc cgccccggac gaactaaacc
     6601 tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt
     6661 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     6721 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     6781 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     6841 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     6901 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     6961 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     7021 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     7081 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     7141 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     7201 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     7261 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     7321 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     7381 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     7441 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     7501 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     7561 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     7621 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     7681 acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc
     7741 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     7801 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     7861 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     7921 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     7981 ggcgggccaa gataggggcg cgattgctgc aatctggagg acaaattaca cacacttgcg
     8041 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
     8101 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
     8161 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     8221 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     8281 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     8341 taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc
     8401 taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     8461 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     8521 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     8581 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     8641 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat
     8701 gcatatacag tcagcatatg atacccagta gtagagtggg agtgctatcc tttgcatatg
     8761 ccgccacctc ccaagggggc gtgaattttc gctgcttgtc cttttcctgc tggttgctcc
     8821 cattcttagg tgaatttaag gaggccaggc taaagccgtc gcatgtctga ttgctcacca
     8881 ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt
     8941 gacaacatgg gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg acaagacaga
     9001 tggccagaaa tacaccaaca gcacgcatga tgtctactgg ggatttattc tttagtgcgg
     9061 gggaatacac ggcttttaat acgattgagg gcgtctccta acaagttaca tcactcctgc
     9121 ccttcctcac cctcatctcc atcacctcct tcatctccgt catctccgtc atcaccctcc
     9181 gcggcagccc cttccaccat aggtggaaac cagggaggca aatctactcc atcgtcaaag
     9241 ctgcacacag tcaccctgat attgcaggta ggagcgggct ttgtcataac aaggtcctta
     9301 atcgcatcct tcaaaacctc agcaaatata tgagtttgta aaaagaccat gaaataacag
     9361 acaatggact cccttagcgg gccaggttgt gggccgggtc caggggccat tccaaagggg
     9421 agacgactca atggtgtaag acgacattgt ggaatagcaa gggcagttcc tcgccttagg
     9481 ttgtaaaggg aggtcttact acctccatat acgaacacac cggcgaccca agttccttcg
     9541 tcggtagtcc tttctacgtg actcctagcc aggagagctc ttaaaccttc tgcaatgttc
     9601 tcaaatttcg ggttggaacc tccttgacca cgatgctttc caaaccaccc tccttttttg
     9661 cgcctgcctc catcaccctg accccggggt ccagtgcttg ggccttctcc tgggtcatct
     9721 gcggggccct gctctatcgc tcccgggggc acgtcaggct caccatctgg gccaccttct
     9781 tggtggtatt caaaataatc ggcttcccct acagggtgga aaaatggcct tctacctgga
     9841 gggggcctgc gcggtggaga cccggatgat gatgactgac tactgggact cctgggcctc
     9901 ttttctccac gtccacgacc tctccccctg gctctttcac gacttccccc cctggctctt
     9961 tcacgtcctc taccccggcg gcctccacta cctcctcgac cccggcctcc actacctcct
    10021 cgaccccggc ctccactgcc tcctcgaccc cggcctccac ctcctgctcc tgcccctcct
    10081 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
    10141 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctcctgct
    10201 cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
    10261 cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgctcctgcc
    10321 cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct
    10381 cctgcccctc ctgctcctgc ccctcctgcc cctcctgccc ctcctgctcc tgcccctcct
    10441 cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct
    10501 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct
    10561 gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct
    10621 gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
    10681 cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct
    10741 cctgcccctc ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg
    10801 caacttggac gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag
    10861 ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca
    10921 tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg
    10981 tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt
    11041 cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg
    11101 cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga
    11161 caaatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac
    11221 tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca
    11281 ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc
    11341 ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct
    11401 gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattaattc
    11461 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
    11521 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
    11581 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
    11641 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
    11701 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
    11761 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
    11821 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
    11881 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
    11941 tcttcctcat ctccgggcct ttcgacctgc aggtcggccg ccacgaccgg tgccgccacc
    12001 atcccctgac ccacgcccct gacccctcac aaggagacga ccttccatga ccgagtacaa
    12061 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
    12121 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
    12181 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
    12241 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
    12301 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
    12361 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
    12421 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
    12481 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
    12541 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
    12601 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
    12661 cccgcagcgc ccgaccgaaa ggagcgcacg accccatggc tccgaccgaa gccacccggg
    12721 gcggccccgc cgaccccgca cccgcccccg aggcccaccg actctagagg atcataatca
    12781 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    12841 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    12901 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    12961 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc taattcggcc
    13021 gcaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    13081 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    13141 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    13201 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    13261 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    13321 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    13381 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    13441 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    13501 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    13561 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttcagg
    13621 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    13681 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    13741 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    13801 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    13861 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    13921 gcctctcccc gcgcgttggc cgattcatta atgcaactat ggccatttaa tgtaaatact
    13981 taagaaaaaa aaccaaatta attttgatac atgctgcatg tgaaagaccc ccgctgacgg
    14041 gtagtcaatc actcagagga gaccctccca aggaacagcg agaccacaag tcggatgcaa
    14101 ctgcaagagg gtttattgga tacacgggta cccgggcgac tcagtcaatc ggaggactgg
    14161 cgccccgagt gaggggttgt gggctctttt attgagctcg gggagcagaa gcgcgcgaac
    14221 agaagcgaga agcgaactga ttggttagtt caaataaggc acagggtcat ttcaggtcct
    14281 tggggcaccc tggaaacatc tgatggttct ctagaaactg ctgagggctg gaccgcatct
    14341 ggggaccatc tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt
    14401 ttggcccata ttcagctgtt ccatctgttc ttggccctga gccggggcag gaactgctta
    14461 ccacagatat cctgtttggc ccatattcag ctgttccatc tgttcctgac cttgatctga
    14521 acttctctat tctcagttat gtatttttcc atgccttgca aaatggcgtt acttaagcta
    14581 gcttgccaaa cctacaggtg gggtctttca ttcccccctt tttctggaga ctaaataaaa
    14641 tcttttattt tatcgtcgac cactg
//
