LOCUS       sequence-354471-       14859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the rat TrkC fused in the C-terminus with mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14859)
  TITLE     Neurotrophin related plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLZRS-TrkC-mCherry
FEATURES             Location/Qualifiers
     source          1..14859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(105..124)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1242..3031
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(3333..5258)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     promoter        5687..6187
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5722..5745)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6120..6139
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6270..6869
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6270..6289)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6766..6786
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7099..7233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7136..7155)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7190..7209
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7356..7943
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7844..7863
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8097..8114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             8244..8713
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(8921..9631)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9199..9218)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9468..9486
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(12293..12309)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12293..12309)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    12348..12722
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(12394..12410)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(12732..13148)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(12748..12764)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(12780..12802)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    13213..13570
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             13635..14104
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        14641..14745
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14746..14814,14815..14859,1..747)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
       61 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      121 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      181 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      241 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      301 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      361 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      421 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      481 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      541 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      601 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
      661 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
      721 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
      781 attgatttgc ggccagatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
      841 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
      901 gccctgttcc acatgtgaca cggggggggg accaaacaca aaggggttct ctgactgtag
      961 ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag
     1021 cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga
     1081 agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag
     1141 gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg
     1201 gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc
     1261 ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg
     1321 ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc
     1381 caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa
     1441 ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa
     1501 tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa
     1561 cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa
     1621 tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc
     1681 ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa
     1741 tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta
     1801 tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca
     1861 gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg
     1921 gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc
     1981 tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg
     2041 agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg
     2101 tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa
     2161 aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg
     2221 cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag
     2281 ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg
     2341 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     2401 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     2461 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     2521 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     2581 catatgctat cctaatagag attagggtag tatatgctat cctaatttat atctgggtag
     2641 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     2701 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     2761 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     2821 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     2881 tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata
     2941 tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg
     3001 gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta ggtgaattta
     3061 aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg tcgctaatgt
     3121 tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat gggtatgccc
     3181 aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga aatacaccaa
     3241 cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac acggctttta
     3301 atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc accctcatct
     3361 ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc cccttccacc
     3421 ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac agtcaccctg
     3481 atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc cttcaaaacc
     3541 tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga ctcccttagc
     3601 gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact caatggtgta
     3661 agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag ggaggtctta
     3721 ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt cctttctacg
     3781 tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt cgggttggaa
     3841 cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc tccatcaccc
     3901 tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc ctgctctatc
     3961 gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta ttcaaaataa
     4021 tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga
     4081 gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc acgtccacga
     4141 cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc tctaccccgg
     4201 cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg
     4261 cctcctcgac cccggcctcc acctcctgct cctgcccctc ctgctcctgc ccctcctcct
     4321 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     4381 cctgcccctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     4441 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct
     4501 gcccctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct
     4561 cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct
     4621 gcccctcctg cccctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct
     4681 gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     4741 cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc
     4801 cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct
     4861 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgcccct
     4921 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcccgctcct
     4981 gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg
     5041 ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt
     5101 cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt
     5161 ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg
     5221 ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa
     5281 tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc
     5341 aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacaaatcca ggtctgaaaa
     5401 ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc
     5461 ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc
     5521 cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag
     5581 gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga
     5641 tcagcttatc gatgataagc tgtcaaacat gagaattaat tctaccgggt aggggaggcg
     5701 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     5761 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     5821 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     5881 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     5941 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     6001 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     6061 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     6121 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     6181 cctttcgacc tgcaggtcgg ccgccacgac cggtgccgcc accatcccct gacccacgcc
     6241 cctgacccct cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg
     6301 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     6361 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     6421 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     6481 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     6541 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     6601 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     6661 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     6721 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     6781 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     6841 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
     6901 aaaggagcgc acgaccccat ggctccgacc gaagccaccc ggggcggccc cgccgacccc
     6961 gcacccgccc ccgaggccca ccgactctag aggatcataa tcagccatac cacatttgta
     7021 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     7081 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7141 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7201 aaactcatca atgtatctta tcatgtctgg atctaattcg gccgcaaaag gatctaggtg
     7261 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7321 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7381 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7441 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7501 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7561 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7621 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7681 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7741 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7801 agcggcaggg tcggaacagg agagcgcacg agggagcttc agggggaaac gcctggtatc
     7861 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7921 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7981 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8041 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8101 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8161 ggccgattca ttaatgcaac tatggccatt taatgtaaat acttaagaaa aaaaaccaaa
     8221 ttaattttga tacatgctgc atgtgaaaga cccccgctga cgggtagtca atcactcaga
     8281 ggagaccctc ccaaggaaca gcgagaccac aagtcggatg caactgcaag agggtttatt
     8341 ggatacacgg gtacccgggc gactcagtca atcggaggac tggcgccccg agtgaggggt
     8401 tgtgggctct tttattgagc tcggggagca gaagcgcgcg aacagaagcg agaagcgaac
     8461 tgattggtta gttcaaataa ggcacagggt catttcaggt ccttggggca ccctggaaac
     8521 atctgatggt tctctagaaa ctgctgaggg ctggaccgca tctggggacc atctgttctt
     8581 ggccctgagc cggggcagga actgcttacc acagatatcc tgtttggccc atattcagct
     8641 gttccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga tatcctgttt
     8701 ggcccatatt cagctgttcc atctgttcct gaccttgatc tgaacttctc tattctcagt
     8761 tatgtatttt tccatgcctt gcaaaatggc gttacttaag ctagcttgcc aaacctacag
     8821 gtggggtctt tcattccccc ctttttctgg agactaaata aaatctttta ttttatcgtc
     8881 gaccactgtg ctggcggccc ctcgagtacg tagcggccgc ttacttgtac agctcgtcca
     8941 tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct
     9001 cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc agctgcacgg
     9061 gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg tccttcagct
     9121 tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg
     9181 aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc
     9241 cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg
     9301 tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg
     9361 acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca
     9421 tgaactgagg ggacaggatg tcccaggcga agggcagggg gccacccttg gtcaccttca
     9481 gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact
     9541 cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc ttgatgatgg
     9601 ccatgttatc ctcctcgccc ttgctcacca tctcgagtac gtaagcagca gcgccaagaa
     9661 tgtccaggta gattggggtg gccttcccca aagcatggag gattttgtaa atctccttaa
     9721 tattcagtcg ctgttgtggt tccctctgcc agcaccccag catgacatca tatacttctt
     9781 ttgggcagac tcggggtctc tccaagacac ggccttgggt gatgcattca atgacctctg
     9841 tgttagagag ctggaaccat ggttgcttcc cataggtaaa gatctcccaa agaataaccc
     9901 caaagctcca cacatcactc tctgtggtaa acttccggta catgatgctt tcaggtggca
     9961 tccagcggat ggggagcatg gtgtgtcctc ccaccctgta gtaatcagta ctgtagacgt
    10021 ccctggacat gccaaaatct ccaatcttca ctagaaggtt ggctccaact aggcagttcc
    10081 tggtggccag gtcccggtgc acaaagtgct gggaggccaa gtacaccatg ccagaggcta
    10141 tctgactggc gatgtggagc atctgggaga gtcctagctc ccccttggcc tggcgtggct
    10201 gtccatccac aaggatcatc gcatctggcc catgggccct gaggaacttg ttcaggtctc
    10261 catgcttcat gtattcaaag accatgatga gtggatcccc atcaccacag accccataga
    10321 atttgacaat atgctcatgc tgcaggttag tgagcagctc ggcctccctc tggaaatcct
    10381 tcctggcagc caaggtggga tccttcaggg ccttcactgc cacaagcatc ttgtctttgg
    10441 tggggcttag attgtagcac tcagccagga agacctttcc aaaggctccc tcacccagtt
    10501 ctctcttcaa cacgatgtct ctcctcttga tgtgttgaac atatgtgtct ggcttgtggc
    10561 aattgtgtcc ctgacggaaa tattgggggt tctcaatgac tggaattcga gtcatgccaa
    10621 tgaccactgt atccggccca gcatccagcg atgaaggtgt agtgatgcca tggttgatat
    10681 ggtgcagtgg gctggctgag tcctcctccc cactgataac agccacagga cccttcattc
    10741 caaatttgga ccgtcgaccg tacttgttga tcatgataaa gaggaccacc agaaggacgc
    10801 aggcaaaagc agcaagtcca actgctatgg acaccccaaa agtgtcttcc tctggtttgt
    10861 gggtcacagt gataggaggt gtagggctcg catcagactc aaagtcaaag aaatctgtgc
    10921 tctctggaaa gggctcctta aggaagtggc cgttgatggt ctggttggct gtgcccaggg
    10981 cattcttagc aatgagggtg tagttgccat tgttgtagtg ggtgggcttg ttgaagagca
    11041 ggcagccctc tgagacttca ccctcctggt agtagtccat atgaatgatc ttggattccc
    11101 tcagtggctg tccattgtac agccaatgca gcgtgggtgt cgggttgcca cgcaccacaa
    11161 actcaatgca atgttccagg cgtacctcag gctccaccaa gctcaccaca cgtggagggt
    11221 agtagacagt gagagcaacg ctggcattgc tcatgcccac cacgttctct gcaatgcacg
    11281 tcagggtgaa accattgtct tcgctcgtca cattcaccag ggtcaagttg atggcatgta
    11341 cattggtcca gttcagattg gtctgatgag tattgatgga ctgcagtccg gtgactatcc
    11401 agtccacatc aggcaaagga gagccagagc cattacaagt gattacagca ttgtctcctt
    11461 ctcggacagt caggttcacg tgactcacac tgatctcagg gagatcacac tgactgatgt
    11521 tcatgcggaa gagggggagt tgggagccat cagcactgat gcagtagagg ctctggctgt
    11581 ccagccgtgc ctccccctgt tcctgccaca gctgcatcca gcggatgtca cagctgcagt
    11641 tgaagaagtt ctgctccagt ctcaattccc gaagactcag cgtctggaag agctgccagg
    11701 agagtgtggt gagccggtta cttgacaagt ttatataacg caagtgggga ttcttagcaa
    11761 aggctctggg ctgaatattc cggagtccag aattcttgat ggtcagcttc tggagtcccg
    11821 tgtagagctc catatccaca gcattgagtg tgtgcaggcc ccgccagttc tctatgtgta
    11881 tggaagtgat attccttgag atgtccgtga tgttgatgct ggcgttccca ttgctgttcc
    11941 ctgaatcctg cccttccagg aggggaaaga ggttcccatc gtccggccgc cggcaattga
    12001 tctcagtctt gctgcagaca caatttgcag ggcaagccag cacggagccc acatagtcca
    12061 gccagacgct tcccagcaag aaaatccgcc agaaactaca cttggctggg caaagagaga
    12121 catccatctc cgatcgctgc ttctaaaaaa gaggaggagg aggagaggag aggggggtgg
    12181 ggtgggggga gtggggagag caggggggga aggaaacaaa gacagcgagg gaggggggaa
    12241 ggcaaaaata ataataataa aaaagctcgt gccgaattcc tgcagcccgg gggatccact
    12301 agttctagag cggccgcgaa ttcccgtacc accacactgg gatccggcag tctagaggat
    12361 ggtccacccc cggggtcggc agccttcacg tgggcggcgt gtatccaagc tgcgatgccg
    12421 tctactttga gggcggtggg ggtggtcagc aggactgtgt aaggtccttt ccagcgaggt
    12481 tctaggttct tagtctggtg tcggcggacc cacactgtgt cgccgactcg gtaagggtga
    12541 ggtaccaccg gtcggtccag ttgttcttgg taggctgccg ccagaggtct ccagacttcg
    12601 tgctggacta agtagagagc ctgtaagtga gcttggagag aggggctgtt agtaactctt
    12661 gtcatgtcag ggtcagggaa gtttacaagg ggcgggggtg ccccatataa gatctcatat
    12721 ggccatatgg gggcgcctag agaaggagtg agggctggat aaagggagga tcgaggcggg
    12781 gtcgaacgag gaggttcaag ggggagagac ggggcggatg gaggaagagg aggcggaggc
    12841 ttagggtgta caaagggctt gacccaggga ggggggtcaa aagccaaggc ttcccaggtc
    12901 acgatgtagg ggacctggtc tgggtgtcca tgcgggccag gtgaaaagac cttgatctta
    12961 acctgggtga tgaggtctcg gttaaaggtg ccgtctcgcg gccatccgac gttaaaggtt
    13021 ggccattctg cagagcagaa ggtaacccaa cgtctcttct tgacatctac cgactggttg
    13081 tgagcgatcc gctcgacatc tttccagtga cctaaggtca aacttaaggg agtggtaaca
    13141 gtctggcccg ggcccatatt ttcagacaaa tacagaaaca cagtcagaca gagacaacac
    13201 agaacgatgc tgcagcagac aagacgcgcg gcgcggcttc ggtcccaaac cgaaagcaaa
    13261 aattcagacg gaggcgggaa ctgttttagg ttctcgtctc ctaccagaac cacatatccc
    13321 tcctctaagg ggggtgcacc aaagagtcca aaacgatcgg gatttttgga ctcaggtcgg
    13381 gccacaaaaa cggcccccga agtccctggg acgtctccca gggttgcggc cgggtgttcc
    13441 gaactcgtca gttccaccac gggtccgcca gatacagagc tagttagcta actagtaccg
    13501 acgcaggcgc ataaaatcag tcatagacac tagacaatcg gacagacaca gataagttgc
    13561 tggccagctt acctcccggt ggtgggtcgg tggtccctgg gcaggggtct cccgatcccg
    13621 gacgagcccc caaatgaaag acccccgctg acgggtagtc aatcactcag aggagaccct
    13681 cccaaggaac agcgagacca caagtcggat gcaactgcaa gagggtttat tggatacacg
    13741 ggtacccggg cgactcagtc aatcggagga ctggcgcgcc gagtgagggg ttgtgggctc
    13801 ttttattgag ctcggggagc agaagcgcgc gaacagaagc gagaagcgaa ctgattggtt
    13861 agttcaaata aggcacaggg tcatttcagg tccttggggc accctggaaa catctgatgg
    13921 ttctctagaa actgctgagg gctggaccgc atctggggac catctgttct tggccctgag
    13981 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
    14041 gttcttggcc ctgagccggg gcaggaactg cttaccacag atatcctgtt tggcccatat
    14101 tcagctgttc catctgttcc tgaccttgat ctgaactttt ctattctcag ttatgtattt
    14161 ttccatgcct tgcaaaatgg cgttacttaa gctagcttgc caaacctaca ggtggggtct
    14221 ttcacatgta tatgtcaaaa ataaaaatca actaattgac tagtaattaa tatgactggc
    14281 ataatgggaa attgatcctg acagatgcaa actggcttct cagcagcgca tttatgttgt
    14341 caactgagga aggaaacgtt aatgacagaa actctaagta atttccacgt ttatctattt
    14401 ttatttatac tagctttggt aacaggaata ttgcagcatt catgcacatt gaaaccctta
    14461 tgaaataaaa acatctgtgc atttaaaatg gaattaacat tttaaatgtt aaaaaaagct
    14521 ggcttagctt ccccccgccc cctagggcat agaacaagtc aaatgcttta tatatttgag
    14581 tttgggatgt attaggaaac tcctaagagc aaagctggtg gcacttttcg gggaaatgtg
    14641 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
    14701 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
    14761 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
    14821 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttg
//
