LOCUS       sequence-354472-        7621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Fan JD, Wagner BL, McDonnell DP
  TITLE     Identification of the sequences within the human complement 3 
            promoter required for estrogen responsiveness provides insight into 
            the mechanism of tamoxifen mixed agonist activity.
  JOURNAL   Mol Endocrinol. 1996 Dec . 10(12):1605-16.
  PUBMED    8961270
REFERENCE   2  (bases 1 to 7621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Endocrinol. 1996 Dec . 10(12):1605-16."
COMMENT     Sequence Label: C3 luc (T1 del)
FEATURES             Location/Qualifiers
     source          1..7621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1220..2872
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1286..1304)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3003..3068
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3198..3218
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3643..3777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3680..3699)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3734..3753
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3795..3811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3795..3811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3808..3830)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3819..3835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3843..3849,3850..3867,3868..3873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3888..3909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4026..4043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4197..4785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4277..4296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4956..5747,5748..5816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5579..5598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5817..5921)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5989..6007
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     6167..6186
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6668..7181)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6863..6884)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7075..7094
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(7607..7621,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(7621,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagcttgcat gccacgcgtc tcgagtaacg caggttcaag
       61 cttattcact gcagtgagct gtgattgcac cactgcaatt tagcctgagt gacagaagga
      121 aaaaaaaatt tttttaaagg aaaacacaaa aagaatatgc tgtcaacagg gatgggagga
      181 agaccacctt tactgctata cacatttgta ccttttagat gttgatcaat atgaatatat
      241 tatacacaca gacacacaca cagacacaca cacacacaca cacaaacaat acaatttaat
      301 atcctaagag gatattgaca ttagacaggt acaaaagctc tagaaatgag gactttcctc
      361 agtgatgact tttttcacca ccaaagtcac tcaggcatcc tgacaagggt aagtgagggg
      421 agcctccttg gaaaataaac tcacttggat agtgaactcc tgcacatacc tcaaagccca
      481 tctgaaatgt cccctcctac aggaagtttt ccctgaccct ccaagaagca gagttctatt
      541 tcactgggga aaacatttct tcttcttctt ttttttccct gccctgcaca tgagctagaa
      601 aacatttcat gaaactggga gttcctgtgc tgggctctgt ccctccccca ttctacttcc
      661 cctccctcag catggaagcc tctggaagtg gggctctgac tcccagccta cagagagatt
      721 cctaggaagt gttcgactga taaacgcatg gccaaaagtg aactggggat gaggtccaag
      781 acatctgcgg tggggggttc tccagacctt agtgttcttc cactacaaag tgggtccaac
      841 agagaaaggt ctgtgctcac caggtggccc tgaccctggg agagtccagg gcagggtgca
      901 gctgcattca tgctgctggg gaacatgccc tcaggttact caccccatgg acatgttggc
      961 cccagggact gaaaagctta ggaaatggta ttgagaaatc tggggcagcc ccaaaagggg
     1021 agaggccatg gggagaaggg ggggctgagt gggggaaagg caggagccag ataaaaagcc
     1081 agctccagca ggcgctgctc actcctcccc atcctctccc tctgtccctc tgtccctctg
     1141 accctgcact gtcccagcag gatccccggg taccgagctc gaatttcgag gagcttggca
     1201 ttccggtact gttggtaaaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt
     1261 ctatcctcta gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
     1321 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc
     1381 ggaatacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
     1441 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
     1501 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
     1561 gctcaacagt atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca
     1621 aaaaattttg aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc
     1681 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
     1741 cggttttaat gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact
     1801 gataatgaat tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac
     1861 tgcctgcgtc agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
     1921 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
     1981 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt
     2041 acgatccctt caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt
     2101 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
     2161 tgggggcgca cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc
     2221 agggatacga caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     2281 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     2341 ggatctggat accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg
     2401 acctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     2461 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     2521 agttgaccgc ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt
     2581 ggaatcgata ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga
     2641 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     2701 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     2761 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     2821 aatcagagag atcctcataa aggccaagaa gggcggaaag tccaaattgt aaaatgtaac
     2881 tgtattcagc gatgacgaaa ttcttagcta ttgtaatact ctagaggatc tttgtgaagg
     2941 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     3001 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     3061 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     3121 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     3181 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     3241 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     3301 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     3361 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
     3421 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
     3481 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
     3541 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     3601 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     3661 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3721 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcggg
     3781 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3841 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3901 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3961 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4021 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4081 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4141 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4201 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4261 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4321 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4381 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4441 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4501 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4561 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4621 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4681 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4741 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4801 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4861 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4921 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4981 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5041 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5101 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5161 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5221 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5281 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5341 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5401 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5461 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5521 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5581 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5641 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5701 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5761 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5821 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5881 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5941 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     6001 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     6061 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     6121 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     6181 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     6241 taccgcatca ggcgattcca acatccaata aatcatacag gcaaggcaaa gaattagcaa
     6301 aattaagcaa taaagcctca gagcataaag ctaaatcggt tgtaccaaaa acattatgac
     6361 cctgtaatac ttttgcggga gaagccttta tttcaacgca aggataaaaa tttttagaac
     6421 cctcatatat tttaaatgca atgcctgagt aatgtgtagg taaagattca aacgggtgag
     6481 aaaggccgga gacagtcaaa tcaccatcaa tatgatattc aaccgttcta gctgataaat
     6541 tcatgccgga gagggtagct atttttgaga ggtctctaca aaggctatca ggtcattgcc
     6601 tgagagtctg gagcaaacaa gagaatcgat gaacggtaat cgtaaaacta gcatgtcaat
     6661 catatgtacc ccggttgata atcagaaaag ccccaaaaac aggaagattg tataagcaaa
     6721 tatttaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     6781 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     6841 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     6901 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     6961 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     7021 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     7081 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     7141 caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
     7201 gcacgtataa cgtgctttcc tcgttagaat cagagcggga gctaaacagg aggccgatta
     7261 aagggatttt agacaggaac ggtacgccag aatcctgaga agtgttttta taatcagtga
     7321 ggccaccgag taaaagagtc tgtccatcac gcaaattaac cgttgtcgca atacttcttt
     7381 gattagtaat aacatcactt gcctgagtag aagaactcaa actatcggcc ttgctggtaa
     7441 tatccagaac aatattaccg ccagccattg caacggaatc gccattcgcc attcaggctg
     7501 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     7561 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     7621 t
//
