LOCUS       sequence-354482-        5226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC4A11_STOP
FEATURES             Location/Qualifiers
     source          1..5226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2783..2882)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2899..2918)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2900..2918)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2923..2939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2923..2939)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3041..3060)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3052..3861
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3119..3138)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4008..4596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4497..4516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4750..4767
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4926..4953)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5045..5131)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5180..5202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5194..5211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5195..5211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgag ccaagtggga
      121 ggaaggggcg ataggtgcac acaggaggtg cagggactgg tgcacggagc aggcgacctg
      181 tccgcctctc tggccgagaa ctcccccacc atgtctcaga atggctattt cgaggatagc
      241 tcctactata agtgtgacac cgacgataca tttgaggcaa gggaggagat cctgggcgac
      301 gaggccttcg ataccgccaa ctctagcatc gtgagcggcg agtccatccg cttctttgtg
      361 aacgtgaatc tggagatgca ggccacaaac accgaaaatg aagcaaccag cggcggatgc
      421 gtgctgctgc acacctctcg caagtacctg aagctgaaga acttcaagga ggagatccgg
      481 gcccacagag acctggatgg cttcctggcc caggccagca tcgtgctgaa cgagacagcc
      541 acctccctgg ataatgtgct gaggaccatg ctgcggagat ttgcccgcga ccctgataac
      601 aatgagccaa actgtaatct ggatctgctg atggcaatgc tgttcacaga cgcaggagca
      661 ccaatgaggg gcaaggtgca cctgctgtcc gacaccatcc agggagtgac agcaaccgtg
      721 acaggcgtgc ggtaccagca gtcttggctg tgcatcatct gtaccatgaa ggccctgcag
      781 aagagacacg tgtgcatctc caggctggtg cgccctcaga actggggaga gaattcttgt
      841 gaggtgcggt tcgtgatcct ggtgctggca ccacctaaga tgaagtctac caagacagcc
      901 atggaggtgg ccagaacctt cgccacaatg tttagcgata tcgcctttcg gcagaagctg
      961 ctggagacca gaacagagga ggagttcaag gaggccctgg tgcaccagag gcagctgctg
     1021 accatggtgt cccacggacc tgtggcacca aggaccaagg agagatctac agtgagcctg
     1081 ccagcacaca ggcaccctga gccacccaag tgcaaggatt tcgtgccttt tggcaagggc
     1141 atcagggagg acatcgcaag gcgcttccca ctgtatcctc tggactttac agatggcatc
     1201 atcggcaaga acaaggccgt gggcaagtat atcaccacaa ccctgttcct gtactttgcc
     1261 tgtctgctgc caaccatcgc ctttggcagc ctgaacgacg agaatacaga tggcgccatc
     1321 gacgtgcaga agaccatcgc aggacagtct atcggaggac tgctgtatgc cctgttcagc
     1381 ggccagcccc tggtcatcct gctgacaacc gcccctctgg ccctgtacat ccaggtcatc
     1441 agagtgatct gcgacgatta tgacctggat ttcaactcct tttacgcctg gacaggcctg
     1501 tggaattctt tctttctggc cctgtacgcc ttctttaatc tgagcctggt catgtccctg
     1561 tttaagagaa gcaccgagga gatcatcgcc ctgttcatct ccatcacatt tgtgctggat
     1621 gccgtgaagg gcaccgtgaa gatcttctgg aagtactatt acggccacta tctggacgat
     1681 taccacacca agaggacatc ctctctggtg agcctgtccg gactgggagc atctctgaac
     1741 gcaagcctgc acaccgccct gaatgcctcc tttctggcct ctcctaccga gctgccaagc
     1801 gccacacact ccggacaggc aaccgccgtg ctgtccctgc tgatcatgct gggcacactg
     1861 tggctgggct ataccctgta ccagttcaag aagagcccct atctgcaccc ttgcgtgagg
     1921 gagatcctgt ccgactgtgc actgccaatc gccgtgctgg ccttctctct gatcagctcc
     1981 cacggctttc gggagatcga gatgagcaag ttcagataca acccatctga gagccccttt
     2041 gccatggccc agatccagtc cctgtctctg agggccgtgt ctggagcaat gggactgggc
     2101 ttcctgctga gcatgctgtt ctttatcgag cagaacctgg tggccgccct ggtgaacgca
     2161 ccagagaatc gcctggtgaa gggcacagcc taccactggg atctgctgct gctggccatc
     2221 atcaataccg gactgagcct gttcggactg ccatggatcc acgcagcata tccacactcc
     2281 ccactgcacg tgagggccct ggccctggtg gaggagaggg tggagaacgg ccacatctac
     2341 gacaccatcg tgaatgtgaa ggagaccaga ctgacatctc tgggagcaag cgtgctggtg
     2401 ggactgtccc tgctgctgct gccagtgcct ctgcagtgga tcccaaagcc cgtgctgtat
     2461 ggcctgtttc tgtacatcgc cctgacaagc ctggacggaa accagctggt gcagagggtg
     2521 gccctgctgc tgaaggagca gaccgcctat cctccaacac actacatccg gagagtgcca
     2581 cagagaaaga tccactattt caccggactg caggtgctgc agctgctgct gctgtgcgcc
     2641 tttggcatgt ctagcctgcc atacatgaag atgatcttcc ccctgatcat gatcgccatg
     2701 atccctatca ggtatatcct gctgccacgc atcatcgagg ccaagtacct ggacgtgatg
     2761 gatgccgagc acaggcctta agacccagct ttcttgtaca aagttggcat tataagaaag
     2821 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     2881 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     2941 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     3001 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     3061 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     3121 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     3181 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     3241 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     3301 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     3361 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     3421 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     3481 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     3541 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     3601 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     3661 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     3721 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     3781 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     3841 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     3901 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     3961 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4021 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4081 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4141 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4201 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4261 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4321 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4381 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4441 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4501 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4561 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4621 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4681 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     4741 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     4801 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     4861 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     4921 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     4981 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     5041 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     5101 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     5161 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     5221 gggccc
//
