LOCUS       sequence-354489-       12115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12115)
  AUTHORS   Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, 
            Schuh M
  TITLE     A Method for the Acute and Rapid Degradation of Endogenous Proteins.
  JOURNAL   Cell. 2017 Nov 13. pii: S0092-8674(17)31255-2. doi: 
            10.1016/j.cell.2017.10.033.
  PUBMED    29153837
REFERENCE   2  (bases 1 to 12115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Nov 13. pii: S0092-8674(17)31255-2. doi: 
            10.1016/j.cell.2017.10.033."
COMMENT     Sequence Label: pSMPP-mCherry-hTRIM21
FEATURES             Location/Qualifiers
     source          1..12115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     42..61
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(82..581)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(130..149)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     523..546
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             613..624
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1022..1042
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      2579..2588
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2595..3302)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2870..2889)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3139..3157
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      3299..3308
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3317..3724)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    3877..3994
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4279..4323)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4508..4741
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5238..5363
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5410..6043
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6211..6345
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6235..6254)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6289..6308
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6770..6790)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6920..6985
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(8119..8448)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8133..8268
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8433..8453
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(8523..8540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8694..9282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8774..8793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9453..10244,10245..10313))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10076..10095
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10314..10418)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10486..10504
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10542..10564)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10664..10683
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10897..10916
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        11206..11224
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     11206..11223
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             11249..11482
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(join(11577..12115,1..61))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11660..11680)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca
       61 tggtaagctt gggctgcagg tcgaaaggcc cggagatgag gaagaggaga acagcgcggc
      121 agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc
      181 cccgcccctg agcccgcccc tgagcccgcc cccggaccca ccccttccca gcctctgagc
      241 ccagaaagcg aaggagcaaa gctgctattg gccgctgccc caaaggccta cccgcttcca
      301 ttgctcagcg gtgctgtcca tctgcacgag actagtgaga cgtgctactt ccatttgtca
      361 cgtcctgcac gacgcgagct gcggggcggg ggggaacttc ctgactaggg gaggagtaga
      421 aggtggcgcg aaggggccac caaagaacgg agccggttgg cgcctaccgg tggatgtgga
      481 atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc
      541 gcatgctcca gactgccttg ggaaaagcgc ctcccctacc cggtagaatt gatgggaagg
      601 aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca
      661 ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga
      721 attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag
      781 ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt
      841 ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg
      901 cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat acaaggagga
      961 gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc
     1021 acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta
     1081 atccagaggt tgattatcga taagcttgat atcaagcttg catgcctgca ggtcgactct
     1141 agagtcgcgg ccgctcaata gtcagtggat ccttgtgatc caatattcag tggacagagg
     1201 gttagagggg ctgtgttttt tcctccatca ttgaaaccag gactgaagaa gggccgcaga
     1261 ggtcctgtaa aggcacattc agagaaggag tagatgaggg agccatggtc agtgatgttg
     1321 tagaaggaga ccatgccagc ctcatagtcc aggaaaatcc caacttggca tggaggcacc
     1381 tgaaggtgga ggggagtctg ggggtaggtg ccagcctcat atttttgttt gttccacaac
     1441 caaattgtcc agaagccact cttggaacta agcaaaaagt gccccttcct gcgcacagag
     1501 tctctgcaga cacccaggtc ccaggcctcc tttcctgtca catctacctc ccagtaatgt
     1561 tttccagagt gaaagtgctg ggcacccagg accataggat aactatcaaa tctctcttca
     1621 tttccaggta tgctctgctg ggtgtctcca agcctcactt gtctccgatc ttctgaaagt
     1681 atcagccacg gattggctgt gtctggatcc agagtgatgt ggactgcaca tgtcctcagc
     1741 atcttcttca gccctggcac atggcacaca ctcctgagtt ctggagaggt aatatccagg
     1801 tccttcaggt tccaggactc actcctttcc aggacaatta tcacctcctg cagcagttcc
     1861 agtgctgagc tgtggcacct tcgatctagc tctgagatga gctcctgtag ggcctggctc
     1921 tgctgggcca gcttggcctc tttctccccc aggattctca gctgctccct ctcatccttc
     1981 tccagctcct gcagctgcct ctgttcttct tcaaccagga agtttttttg ctgcacaaac
     2041 tctgcgtgaa tcctagattt ctgtgtttcc actgttttct tccagtctgc tctctttatt
     2101 gcaatttcca cttccaactt ctcagccaac tcctgctttc ttctcagttc ccctaatgcc
     2161 acctggagct tctcctggta ctcctgtgca gcctcctcaa gagggaccat ggcgtggtca
     2221 cggtgtttcc gagactgggc acatacccag caaagggcct tcccatcttt ctcacagaac
     2281 aggtgaagtc tctctccatg cactgcacac cgttccccct gtgtgccctc tctggcctcc
     2341 tggctgattt ctttaaggtt gttcaccatg ttggctagct gtcgattggg ccggagattc
     2401 ttgagcagaa agcgctgccg gcacacagga cagacgctgc ccccaccttt cccaacctga
     2461 gagatgcatt cctggcagaa gctgtggcca cactcgatgc tcacaggctc cacgaagggg
     2521 tccaggcaga tagggcatgt gacctcctcc cacatcattg tcaagcgtgc tgctgaagcc
     2581 atggtggcac gcgtcttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg
     2641 cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg
     2701 ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc
     2761 tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg
     2821 gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc
     2881 attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg
     2941 ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc
     3001 acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg
     3061 gggtgcttca cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg
     3121 aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
     3181 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc
     3241 accttgaagc gcatgaactc cttgatgatg gccatgttat cctcctcgcc cttgctcacc
     3301 atggtggcgg atcccccccg ggcgactcag tctgtcggag gactggcgcg ccgagtgagg
     3361 ggttgtgagc tcttttatag agctcgggaa gcagaagcgc gcgaacagaa gcgagaagca
     3421 ggctgattgg ttaattcaaa taaggcgcag ggtcatttca ggtccttggg ggagcctgga
     3481 aacatctgat gggtcttaag aaactgctga gggttgggcc atatctgggg accatctgtt
     3541 cttggcctcg ggccggggcc gaaactgcgg tgaccatctg ttcttggccc cgggccgggg
     3601 ccgaaactgc tcaccgcaga tatcctgttt ggcccaacgt tagctatttt catgtacccg
     3661 cccttgatct gaacttctct attcttggtt tggtattttt ccatgccttg caaaatggcg
     3721 ttactgcagc tagcttgcca aacctacagg tggggtcttt cattcccccc tttttctgga
     3781 gactaaataa aatcttttat tttatcgggg ctgcaggaat tcgatatcaa gcttatcgat
     3841 ccaaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg
     3901 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
     3961 actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttggcg
     4021 ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc
     4081 ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga
     4141 taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct
     4201 taaacctacc aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt
     4261 tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt
     4321 cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt
     4381 aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt
     4441 actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca
     4501 aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag
     4561 ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag
     4621 caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt
     4681 cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc
     4741 tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac
     4801 tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc aattgtccct
     4861 catatctcct cctccaggtc tgaagatcag cggccggccg cttgctgtgc ggtggtctta
     4921 cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt tatctctatc
     4981 ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag ttcttctgat
     5041 cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc tgatgtttct
     5101 aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac tatatgtttt
     5161 aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg cgatctaatt
     5221 ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc tccgctagtc
     5281 aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca
     5341 agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa gtccctgttc
     5401 gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     5461 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     5521 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     5581 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
     5641 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag
     5701 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga
     5761 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca
     5821 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta
     5881 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct
     5941 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga
     6001 tcaaggatat cttgtcttct ttgggagtga attagccctt ccaactacta agtttgtagt
     6061 acatatttaa caaatacaat ttctttaaaa tgaaaataat tcagaggaat cacaggttta
     6121 gagtaaatga aaccacaggt aattggcagt ggtaataggg tatggggtgg gaagtttggg
     6181 atgattttgg ttagcttgag ttatccagtt gatccagaca tgataagata cattgatgag
     6241 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     6301 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     6361 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     6421 ctctacaaat gtggtatggc tgattatgat ctctagtcaa ggcactatac atcaaatatt
     6481 ccttattaac ccctttacaa attaaaaagc taaaggtaca caatttttga gcatagttat
     6541 taatagcaga cactctatgc ctgtgtggag taagaaaaaa cagtatgtta tgattataac
     6601 tgttatgcct acttataaag gttacagaat atttttccat aattttcttg tatagcagtg
     6661 cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag
     6721 catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct
     6781 tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca
     6841 tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat
     6901 cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt
     6961 atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca
     7021 attatgtcac accacagaag taaggttcct tcacaaagat ccggggccca ctcataaatc
     7081 cagttgccgc cacggtagcc aatcaccgta tcgtataaat catcgtcggt acgttcggca
     7141 tcgctcatca caatacgtgc ctggacgtcg aggatttcgc gtgggtcaat gccgcgccag
     7201 atccacatca gacggttaat catgcgatac cagtgaggga tggttttacc atcaagggcc
     7261 gactgcacag gcggttgtgc gccgtgatta aagcggcgga ctagcgtcga ggtttcagga
     7321 tgtttaaagc ggggtttgaa cagggtttcg ctcaggtttg cctgtgtcat ggatgcagcc
     7381 tccagaatac ttactggaaa ctattgtaac ccgcctgaag ttaaaaagaa caacgcccgg
     7441 cagtgccagg cgttgaaaag attagcgacc ggagattggc gggacgaata cgacgcccat
     7501 atcccacggc tgttcaatcc aggtatcttg cgggatatca acaacatagt catcaaccag
     7561 cggacgacca gccggttttg cgaagatggt gacaaagtgc gcttttggat acatttcacg
     7621 aatcgcaacc gcagtaccac cggtatccac caggtcatca ataacgatga agccttcgcc
     7681 atcgccttct gcgcgtttca gcactttaag ctcgcgctgg ttgtcgtgat cgtagctgga
     7741 aatacaaacg gtatcgacat gacgaatacc cagttcacgc gccagtaacg cacccggtac
     7801 agaccgccac ggcttacggc aataatgcct ttccattgtt cagaaggcat cagtcggctt
     7861 gcgagtttac gtgcatggat ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg
     7921 tgaagtgtcc cagcctgttt atctacggct taaaaagtgt tcgaggggaa aataggttgc
     7981 gcgagattat agagatctgg cgcactaaaa accagtattt cacatgagtc cgcgtctttt
     8041 tacgcactgc ctctccctga cgcgggataa agtggtattc tcaaacatat ctcgcaagcc
     8101 tgtcttgtgt ccaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     8161 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     8221 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     8281 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     8341 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     8401 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     8461 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8521 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8581 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8641 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8701 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8761 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8821 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8881 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8941 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9001 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9061 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9121 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9181 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9241 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9301 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9361 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9421 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9481 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9541 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9601 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9661 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9721 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9781 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9841 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9901 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9961 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10021 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10081 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10141 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10201 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10261 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10321 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10381 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10441 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    10501 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
    10561 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
    10621 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
    10681 gtactgagag tgcaccattc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
    10741 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
    10801 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
    10861 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
    10921 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctg
    10981 gctagcgatg accctgctga ttggttcgct gaccatttcc gggtgcggga cggcgttacc
    11041 agaaactcag aaggttcgtc caaccaaacc gactctgacg gcagtttacg agagagatga
    11101 tagggtctgc ttcagtaagc cagatgctac acaattaggc ttgtacatat tgtcgttaga
    11161 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
    11221 tagaatacac ggaattaatt ctagatgctg ctagagattt tccacactga ctaaaagggt
    11281 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
    11341 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
    11401 gctcagatct ggtctaacca gagagaccca gtacaagcaa aaagcagatc ttgtcttcgt
    11461 tgggagtgaa ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca
    11521 gctgccttgt aagtcattgg tcttaaaggt accgagctcg aattaattcc ctcgagtcag
    11581 gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg
    11641 gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga ggtctccagg
    11701 aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac ggcgctgccc
    11761 agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca cgcgggctcc
    11821 ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag ccgggaaccg
    11881 ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc gacgctctcc
    11941 ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg
    12001 agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat gtggcggtcc
    12061 ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag ggtgc
//
