LOCUS       sequence-354504-       17531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to overexpress human MALAT1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17531)
  AUTHORS   Hu Y, Lin J, Fang H, Fang J, Li C, Chen W, Liu S, Ondrejka S, Gong 
            Z, Reu F, Maciejewski J, Yi Q, Zhao JJ
  TITLE     Targeting the MALAT1/PARP1/LIG3 complex induces DNA damage and 
            apoptosis in multiple myeloma.
  JOURNAL   Leukemia. 2018 Mar 22. pii: 10.1038/s41375-018-0104-2. doi: 
            10.1038/s41375-018-0104-2.
  PUBMED    29632340
REFERENCE   2  (bases 1 to 17531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Leukemia. 
            2018 Mar 22. pii: 10.1038/s41375-018-0104-2. doi: 
            10.1038/s41375-018-0104-2."
COMMENT     Sequence Label: pCDH-hMALAT1
FEATURES             Location/Qualifiers
     source          1..17531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5525..5736
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5749..6017
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      6046..6055
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6076..6738
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             6808..6861
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6862..7461
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6862..6881)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7358..7378
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7462..8050
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7515..7535)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7933..7944)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8124..8357
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8429..8550
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8466..8485)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8520..8539
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8590..8725
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8758..8774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8758..8774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8771..8793)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8782..8798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8806..8812,8813..8830,8831..8836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8851..8872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8989..9006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9160..9748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9919..10710,10711..10779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10542..10561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10780..10884)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10952..10970
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11008..11030)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11130..11149
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11343..11365
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11357..11374
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11358..11374
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11389..11615
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             11616..11796
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11843..11968
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12461..12694
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12879..12923
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13190..13306
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        13756..13967
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             13980..14248
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
ORIGIN
        1 ccaaatgaat ttgatagcca aattgagaca atttcagcaa atctgtaagc agtttgtatg
       61 tttagttggg gtaatgaagt atttcagttt tgtgaataga tgacctgttt ttacttcctc
      121 accctgaatt cgttttgtaa atgtagagtt tggatgtgta actgaggcgg gggggagttt
      181 tcagtatttt tttttgtggg ggtgggggca aaatatgttt tcagttcttt ttcccttagg
      241 tctgtctaga atcctaaagg caaatgactc aaggtgtaac agaaaacaag aaaatccaat
      301 atcaggataa tcagaccacc acaggtttac agtttataga aactagagca gttctcacgt
      361 tgaggtctgt ggaagagatg tccattggag aaatggctgg tagttactct tttttccccc
      421 caccccctta atcagacttt aaaagtgctt aaccccttaa acttgttatt ttttacttga
      481 agcattttgg gatggtctta acagggaaga gagagggtgg gggagaaaat gtttttttct
      541 aagattttcc acagatgcta tagtactatt gacaaactgg gttagagaag gagtgtaccg
      601 ctgtgctgtt ggcacgaaca ccttcaggga ctggagctgc ttttatcctt ggaagagtat
      661 tcccagttga agctgaaaag tacagcacag tgcagctttg gttcatattc agtcatctca
      721 ggagaacttc agaagagctt gagtaggcca aatgttgaag ttaagttttc caataatgtg
      781 acttcttaaa agttttatta aaggggaggg gcaaatattg gcaattagtt ggcagtggcc
      841 tgttacggtt gggattggtg gggtgggttt aggtaattgt ttagtttatg attgcagata
      901 aactcatgcc agagaactta aagtcttaga atggaaaaag taaaggaata tcaacttcca
      961 agttggcaag taactcccaa tgatttagtt tttttccccc cagtttgaat tgggaagctg
     1021 ggggaagtta aatatgagcc actgggtgta ccagtgcatt aatttgggca aggaaagtgt
     1081 cataatttga tactgtatct gttttccttc aaagtataga gcttttgggg aaggaaagta
     1141 ttgaactggg ggttggtctg gcctactggg ctgacattaa ctacaattat gggaaatgca
     1201 aaagttgttt ggatatggta gtgtgtggtt ctcttttgga atttttttca ggtgatttaa
     1261 taataattta aaactactat agaaactgca gagcaaagga agtggcttaa tgatcctgaa
     1321 gggatttctt ctgatggtag cttttgtatt atcaagtaag attctatttt cagttgtgtg
     1381 tatgcaagtt tttttttagt gtaggagaaa tacttttcca ttgtttaact gcaaaacaag
     1441 atgttaaggt atgcttcaaa aattttgtaa attgtttatt ttaaacttat ctgtttgtaa
     1501 attgtaactg attaagaatt gtgatagttc agcttgaatg tctcttagag ggtgggcttt
     1561 tgttgatgag ggaggggaaa cttttttttt ttctatagac ttttttcaga taacatcttc
     1621 tgagtcataa ccagcctggc agtatgatgg cctagatgca gagaaaacag ctccttggtg
     1681 aattgataag taaaggcaga aaagattata tgtcatacct ccattgggga ataagcataa
     1741 ccctgagatt cttactactg atgagaacat tatctgcata tgccaaaaaa ttttaagcaa
     1801 atgaaagcta ccaatttaaa gttacggaat ctaccatttt aaagttaatt gcttgtcaag
     1861 ctataaccac aaaaataatg aattgatgag aaatacaatg aagaggcaat gtccatctca
     1921 aaatactgct tttacaaaag cagaataaaa gcgaaaagaa atgaaaatgt tacactacat
     1981 taatcctgga ataaaagaag ccgaaataaa tgagagatga gttgggatca agtggattga
     2041 ggaggctgtg ctgtgtgcca atgtttcgtt tgcctcagac aggtatctct tcgttatcag
     2101 aagagttgct tcatttcatc tgggagcaga aaacagcagg cagctgttaa cagataagtt
     2161 taacttgcat ctgcagtatt gcatgttagg gataagtgct tatttttaag agctgtggag
     2221 ttcttaaata tcaaccatgg cactttctcc tgaccccttc cctaggggat ttcaggattg
     2281 agaaattttt ccatcgagcc tttttaaaat tgtaggactt gttcctgtgg gcttcagtga
     2341 tgggatagta cacttcactc agaggcattt gcatctttaa ataatttctt aaaagcctct
     2401 aaagtgatca gtgccttgat gccaactaag gaaatttgtt tagcattgaa tctctgaagg
     2461 ctctatgaaa ggaatagcat gatgtgctgt tagaatcaga tgttactgct aaaatttaca
     2521 tgttgtgatg taaattgtgt agaaaaccat taaatcattc aaaataataa actattttta
     2581 ttagagaatg tatactttta gaaagctgtc tccttattta aataaaatag tgtttgtctg
     2641 tagttcagtg ttggggcaat cttggggggg attcttctct aatctttcag aaactttgtc
     2701 tgcgaacact ctttaatgga ccagatcagg atttgagcgg aagaacgaat gtaactttaa
     2761 ggcaggaaag acaaatttta ttcttcataa agtgatgagc atataataat tccaggcaca
     2821 tggcaataga ggccctctaa ataaggaata aataacctct tagacaggtg ggagattatg
     2881 atcagagtaa aaggtaatta cacattttat ttccagaaag tcaggggtct ataaattgac
     2941 agtgattaga gtaatacttt ttcacatttc caaagtttgc atgttaactt taaatgctta
     3001 caatcttaga gtggtaggca atgttttaca ctattgacct tatataggga agggaggggg
     3061 tgcctgtggg gttttaaaga attttccttt gcagaggcat ttcatccttc atgaagccat
     3121 tcaggatttt gaattgcata tgagtgcttg gctcttcctt ctgttctagt gagtgtatga
     3181 gaccttgcag tgagtttatc agcatactca aaattttttt cctggaattt ggagggatgg
     3241 gaggaggggg tggggcttac ttgttgtagc tttttttttt tacagacttc acagagaatg
     3301 cagttgtctt gacttcaggt ttgtctgttc tgttggcaag taaatgcagt actgttctga
     3361 tcccgctgct attagaatgc attgtgaaac gactggagta tgattaaaag ttgtgttccc
     3421 caatgcttgg agtagtgatt gttgaaggaa aaaatccagc tgagtgataa aggctgagtg
     3481 ttgaggaaat ttctgcagtt ttaagcagtc gtatttgtga ttgaagctga gtacattttg
     3541 ctggtgtatt tttaggtaaa atgctttttg ttcatttctg gtggtgggag gggactgaag
     3601 cctttagtct tttccagatg caaccttaaa atcagtgaca agaaacattc caaacaagca
     3661 acagtcttca agaaattaaa ctggcaagtg gaaatgttta aacagttcag tgatctttag
     3721 tgcattgttt atgtgtgggt ttctctctcc cctcccttgg tcttaattct tacatgcagg
     3781 aacactcagc agacacacgt atgcgaaggg ccagagaagc cagacccagt aagaaaaaat
     3841 agcctattta ctttaaataa accaaacatt ccattttaaa tgtggggatt gggaaccact
     3901 agttctttca gatggtattc ttcagactat agaaggagct tccagttgaa ttcaccagtg
     3961 gacaaaatga ggaaaacagg tgaacaagct ttttctgtat ttacatacaa agtcagatca
     4021 gttatgggac aatagtattg aatagatttc agctttatgc tggagtaact ggcatgtgag
     4081 caaactgtgt tggcgtgggg gtggaggggt gaggtgggcg ctaagccttt ttttaagatt
     4141 tttcaggtac ccctcactaa aggcaccgaa ggcttaaagt aggacaacca tggagccttc
     4201 ctgtggcagg agagacaaca aagcgctatt atcctaaggt caagagaagt gtcagcctca
     4261 cctgattttt attagtaatg aggacttgcc tcaactccct ctttctggag tgaagcatcc
     4321 gaaggaatgc ttgaagtacc cctgggcttc tcttaacatt taagcaagct gtttttatag
     4381 cagctcttaa taataaagcc caaatctcaa gcggtgcttg aaggggaggg aaagggggaa
     4441 agcgggcagc cacttttccc tagcttttcc agaagcctgt taaaagcaag gtctccccac
     4501 aagcaacttc tctgccacat cgccaccccg tgccttttga tctagcacag acccttcacc
     4561 cctcacctcg atgcagccag tagcttggat ccttgtgggc atgatccata atcggtttca
     4621 aggtaacgat ggtgtcgagg tctttggtgg gttgaactat gttagaaaag gccattaatt
     4681 tgcctgcaaa ttgttaacag aagggtatta aaaccacagc taagtagctc tattataata
     4741 cttatccagt gactaaaacc aacttaaacc agtaagtgga gaaataacat gttcaagaac
     4801 tgtaatgctg ggtgggaaca tgtaacttgt agactggaga agataggcat ttgagtggct
     4861 gagagggctt ttgggtggga atgcaaaaat tctctgctaa gactttttca ggtgaacata
     4921 acagacttgg ccaagctagc atcttagcgg aagctgatct ccaatgctct tcagtagggt
     4981 catgaaggtt tttcttttcc tgagaaaaca acacgtattg ttttctcagg ttttgctttt
     5041 tggccttttt ctagcttaaa aaaaaaaaaa gcaaaagatg ctggtggttg gcactcctgg
     5101 tttccaggac ggggttcaga tccctgcggc gtctttgctt tgactactaa tctgtcttca
     5161 ggactctttc tgtatttctc cttttctctg caggtgctag ttcttggagt tttggggagg
     5221 tgggagataa cagcacaata tctttgaact atatacatcc ttgatgtata atttgtcagg
     5281 agcttgactt gattgtatat tcatatttac acgagaacct aatataactg ccttgtcttt
     5341 ttcaggtaat agcctgcagc tggtgttttg agaagcccta ctgctgaaaa cttaacaatt
     5401 ttgtgtaata aaaatggaga agctctaaat tgttgtggtt cttttgtgaa taaaaaaatc
     5461 ttgattgggg aaaaaaaaac tcgagggccg cgaaggatct gcgatcgctc cggtgcccgt
     5521 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5581 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5641 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5701 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     5761 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     5821 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     5881 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     5941 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     6001 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     6061 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     6121 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     6181 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     6241 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     6301 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     6361 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     6421 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     6481 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
     6541 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
     6601 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     6661 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     6721 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     6781 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
     6841 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     6901 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6961 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     7021 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     7081 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     7141 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7201 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7261 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7321 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7381 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7441 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     7501 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7561 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7621 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7681 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7741 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7801 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7861 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7921 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7981 ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttgggcc
     8041 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     8101 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
     8161 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8221 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8281 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8341 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     8401 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     8461 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8521 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     8581 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8641 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8701 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
     8761 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8821 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8881 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8941 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9001 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9061 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9541 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9601 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9841 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9901 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9961 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    10021 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    10081 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    10141 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    10201 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10261 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10321 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10381 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10441 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10501 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10561 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10621 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10681 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10741 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10861 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    10921 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    10981 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    11041 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    11101 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    11161 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    11221 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    11281 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    11341 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctgacgcgtg tagtcttatg
    11401 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
    11461 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
    11521 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
    11581 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
    11641 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
    11701 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
    11761 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
    11821 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
    11881 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
    11941 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
    12001 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
    12061 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
    12121 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
    12181 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
    12241 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
    12301 gcaagcggcc actgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
    12361 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
    12421 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
    12481 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
    12541 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
    12601 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
    12661 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
    12721 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
    12781 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
    12841 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
    12901 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
    12961 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
    13021 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
    13081 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
    13141 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
    13201 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
    13261 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg atactagttg
    13321 aaagacccca cctgtaggtt tggcaagtta gcttaagtaa cgccattttg caaggcatgg
    13381 aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag agacagcaga
    13441 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
    13501 agatggtccc cagatgcggt cccgccctca gcagtttcta gcgaaccatc agatgtttcc
    13561 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagtttgc
    13621 ttcttgcttc tgtttgtgtg cttctgctcc ctgagctcaa taaaagagcc cacaacccct
    13681 cacttggtgg gccagtcctc tgatagactg tgtcccctgg atacccgtat tctaggcggc
    13741 cgcgtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
    13801 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
    13861 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
    13921 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag
    13981 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt
    14041 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg
    14101 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta
    14161 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt
    14221 cgttttctgt tctgcgccgt tacagatcgc ggccgcgtaa aggactgggg ccccgcaact
    14281 ggcctctcct gccctcttaa gcgcagcgcc attttagcaa cgcagaagcc cggcgccggg
    14341 aagcctcagc tcgcctgaag gcaggtcccc tctgacgcct ccgggagccc aggtttccca
    14401 gagtccttgg gacgcagcga cgagttgtgc tgctatctta gctgtcctta taggctggcc
    14461 attccaggtg gtggtattta gataaaacca ctcaaactct gcagtttggt cttggggttt
    14521 ggaggaaagc ttttattttt cttcctgctc cggttcagaa ggtctgaagc tcatacctaa
    14581 ccgggcataa cacagaatct gcaaaacaaa aacccctaaa aaagcagacc cagagcagtg
    14641 taaacacttc tgggtgtgtc cctgactggc tgcccaaggt ctctgtgtct tcggagacaa
    14701 agccattcgc ttagttggtc tactttaaaa ggccacttga actcgctttc catggcgatt
    14761 tgccttgtga gcactttcag gagagcctgg aagctgaaaa acggtagaaa aatttccgtg
    14821 cgggccgtgg ggggctggcg gcaactgggg ggccgcagat cagagtgggc cactggcagc
    14881 caacggcccc cggggctcag gcggggagca gctctgtggt gtgggattga ggcgttttcc
    14941 aagagtgggt tttcacgttt ctaagatttc ccaagcagac agcccgtgct gctccgattt
    15001 ctcgaacaaa aaagcaaaac gtgtggctgt cttgggagca agtcgcagga ctgcaagcag
    15061 ttgggggaga aagtccgcca ttttgccact tctcaaccgt ccctgcaagg ctggggctca
    15121 gttgcgtaat ggaaagtaaa gccctgaact atcacacttt aatcttcctt caaaaggtgg
    15181 taaactatac ctactgtccc tcaagagaac acaagtgctt taagaggtat tttaaaagtt
    15241 ccgggggttt tgtgaggtgt ttgatgaccc gtttaaaata tgatttccat gtttcttttg
    15301 tctaaagttt gcagctcaaa tctttccaca cgctagtaat ttaagtattt ctgcatgtgt
    15361 agtttgcatt caagttccat aagctgttaa gaaaaatcta gaaaagtaaa actagaacct
    15421 atttttaacc gaagaactac tttttgcctc cctcacaaag gcggcggaag gtgatcgaat
    15481 tccggtgatg cgagttgttc tccgtctata aatacgcctc gcccgagctg tgcggtaggc
    15541 attgaggcag ccagcgcagg ggcttctgct gagggggcag gcggagcttg aggaaaccgc
    15601 agatgagttt ttttctcttt gaaagataga gattaataca actacttaaa aaatatagtc
    15661 aataggttac taagatattg cttagcgtta agtttttaac gtaattttaa tagcttaaga
    15721 ttttaagaga aaatatgaag acttagaaga gtagcatgag gaaggaaaag ataaaaggtt
    15781 tctaaaacat gacggaggtt gagatgaagc ttcttcatgg agtaaaaaat gtatttaaaa
    15841 gaaaattgag agaaaggact acagagcccc gaattaatac caatagaagg gcaatgcttt
    15901 tagattaaaa tgaaggtgac ttaaacagct taaagtttag tttaaaagtt gtaggtgatt
    15961 aaaataattt gaaggcgatc ttttaaaaag agattaaact gaaggtgatt aaaagacctt
    16021 gaaatccatg acgcagggag aattgcgtca tttaaagcct agttaacgca tttactaaac
    16081 gcagacgaaa atggaaagat taattgggag tggtaggatg aaacaatttg gagaagatag
    16141 aagtttgaag tggaaaactg gaagacagaa gtacgggaag gcgaagaaaa gaatagagaa
    16201 gatagggaaa ttagaagata aaaacatact tttagaagaa aaaagataaa tttaaacctg
    16261 aaaagtagga agcagaagaa aaaagacaag ctaggaaaca aaaagctaag ggcaaaatgt
    16321 acaaacttag aagaaaattg gaagatagaa acaagataga aaatgaaaat attgtcaaga
    16381 gtttcagata gaaaatgaaa aacaagctaa gacaagtatt ggagaagtat agaagataga
    16441 aaaatataaa gccaaaaatt ggataaaata gcactgaaaa aatgaggaaa ttattggtaa
    16501 ccaatttatt ttaaaagccc atcaatttaa tttctggtgg tgcagaagtt agaaggtaaa
    16561 gcttgagaag atgagggtgt ttacgtagac cagaaccaat ttagaagaat acttgaagct
    16621 agaaggggaa gttggttaaa aatcacatca aaaagctact aaaaggactg gtgtaattta
    16681 aaaaaaacta aggcagaagg cttttggaag agttagaaga atttggaagg ccttaaatat
    16741 agtagcttag tttgaaaaat gtgaaggact ttcgtaacgg aagtaattca agatcaagag
    16801 taattaccaa cttaatgttt ttgcattgga ctttgagtta agattatttt ttaaatcctg
    16861 aggactagca ttaattgaca gctgacccag gtgctacaca gaagtggatt cagtgagtct
    16921 aggaagacag cagcagacag gattccagga accagtgttt gatgaagcta ggactgagga
    16981 gcaagcgagc aagcagcagt tcgtggtgaa gataggaaaa gagtccagga gccagtgcga
    17041 tttggtgaag gaagctagga agaaggaagg agcgctaacg atttggtggt gaagctagga
    17101 aaaaggattc caggaaggag cgagtgcaat ttggtgatga aggtagcagg cggcttggct
    17161 tggcaaccac acggaggagg cgagcaggcg ttgtgcgtag aggatcctag accagcatgc
    17221 cagtgtgcca aggccacagg gaaagcgagt ggttggtaaa aatccgtgag gtcggcaata
    17281 tgttgttttt ctggaactta cttatggtaa ccttttattt attttctaat ataatggggg
    17341 agtttcgtac tgaggtgtaa agggatttat atggggacgt aggccgattt ccgggtgttg
    17401 taggtttctc tttttcaggc ttatactcat gaatcttgtc tgaagctttt gagggcagac
    17461 tgccaagtcc tggagaaata gtagatggca agtttgtggg tttttttttt tttacacgaa
    17521 tttgaggaaa a
//
