LOCUS       sequence-354505-       12517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12517)
  AUTHORS   Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D
  TITLE     Reversible immortalization of human primary cells by 
            lentivector-mediated transfer of specific genes.
  JOURNAL   Mol Ther. 2000 Oct . 2(4):404-14.
  PUBMED    11020357
REFERENCE   2  (bases 1 to 12517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2000 Oct . 2(4):404-14."
COMMENT     Sequence Label: pLOX-Ttag-iresTK
FEATURES             Location/Qualifiers
     source          1..12517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..30)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     65..84
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(546..566)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          696..761
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(1895..2224)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1909..2044
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2209..2229
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(2299..2316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2470..3058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2550..2569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3229..4020,4021..4089))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3852..3871
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4090..4194)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4262..4280
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4318..4340)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4440..4459
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4673..4692
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        4982..5000
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4982..4999
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             5025..5205
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    5225..5258
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5414..6544)
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     misc_feature    6549..7010
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6708..6727)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6937..6954
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7058..7074
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7059..7075
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(join(7128..9017,9364..9609))
                     /codon_start=1
                     /product="SV40 (simian virus 40) large T antigen"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGVGVLDWLRNSDDD
                     DDDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQPSQSSQSVHDHNQPYHICR
                     GFTCFKKPPTPPPEPET"
     intron          complement(9018..9363)
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     primer_bind     complement(9769..9793)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9790..9993)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9819..9839)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9994..10297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(10576..10620)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10805..11038
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    11531..11656
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             11703..12336
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    join(12504..12517,1..121)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
       61 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
      121 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
      181 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatctct agtcaaggca
      241 ctatacatca aatattcctt attaacccct ttacaaatta aaaagctaaa ggtacacaat
      301 ttttgagcat agttattaat agcagacact ctatgcctgt gtggagtaag aaaaaacagt
      361 atgttatgat tataactgtt atgcctactt ataaaggtta cagaatattt ttccataatt
      421 ttcttgtata gcagtgcagc tttttccttt gtggtgtaaa tagcaaagca agcaagagtt
      481 ctattactaa acacagcatg actcaaaaaa cttagcaatt ctgaaggaaa gtccttgggg
      541 tcttctacct ttctcttctt ttttggagga gtagaatgtt gagagtcagc agtagcctca
      601 tcatcactag atggcatttc ttctgagcaa aacaggtttt cctcattaaa ggcattccac
      661 cactgctccc attcatcagt tccataggtt ggaatctaaa atacacaaac aattagaatc
      721 agtagtttaa cacattatac acttaaaaat tttatattta ccttagagct ttaaatctct
      781 gtaggtagtt tgtccaatta tgtcacacca cagaagtaag gttccttcac aaagatccgg
      841 ggcccactca taaatccagt tgccgccacg gtagccaatc accgtatcgt ataaatcatc
      901 gtcggtacgt tcggcatcgc tcatcacaat acgtgcctgg acgtcgagga tttcgcgtgg
      961 gtcaatgccg cgccagatcc acatcagacg gttaatcatg cgataccagt gagggatggt
     1021 tttaccatca agggccgact gcacaggcgg ttgtgcgccg tgattaaagc ggcggactag
     1081 cgtcgaggtt tcaggatgtt taaagcgggg tttgaacagg gtttcgctca ggtttgcctg
     1141 tgtcatggat gcagcctcca gaatacttac tggaaactat tgtaacccgc ctgaagttaa
     1201 aaagaacaac gcccggcagt gccaggcgtt gaaaagatta gcgaccggag attggcggga
     1261 cgaatacgac gcccatatcc cacggctgtt caatccaggt atcttgcggg atatcaacaa
     1321 catagtcatc aaccagcgga cgaccagccg gttttgcgaa gatggtgaca aagtgcgctt
     1381 ttggatacat ttcacgaatc gcaaccgcag taccaccggt atccaccagg tcatcaataa
     1441 cgatgaagcc ttcgccatcg ccttctgcgc gtttcagcac tttaagctcg cgctggttgt
     1501 cgtgatcgta gctggaaata caaacggtat cgacatgacg aatacccagt tcacgcgcca
     1561 gtaacgcacc cggtacagac cgccacggct tacggcaata atgcctttcc attgttcaga
     1621 aggcatcagt cggcttgcga gtttacgtgc atggatctgc aacatgtccc aggtgacgat
     1681 gtatttttcg ctcatgtgaa gtgtcccagc ctgtttatct acggcttaaa aagtgttcga
     1741 ggggaaaata ggttgcgcga gattatagag atctggcgca ctaaaaacca gtatttcaca
     1801 tgagtccgcg tctttttacg cactgcctct ccctgacgcg ggataaagtg gtattctcaa
     1861 acatatctcg caagcctgtc ttgtgtccaa gctttttgca aaagcctagg cctccaaaaa
     1921 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa
     1981 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg
     2041 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
     2101 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat
     2161 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
     2221 acacattcca cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2281 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2341 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2401 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2461 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2521 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2581 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2641 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2701 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2761 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2821 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2881 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2941 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3001 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3061 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3121 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3181 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3241 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3301 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3361 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3421 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3481 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3541 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3601 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3661 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3721 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3781 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3841 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3901 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3961 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4021 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4081 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4141 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4201 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     4261 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     4321 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     4381 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     4441 ggcatcagag cagattgtac tgagagtgca ccattcgacg ctctccctta tgcgactcct
     4501 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
     4561 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     4621 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     4681 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     4741 cggcgtagag gatctggcta gcgatgaccc tgctgattgg ttcgctgacc atttccgggt
     4801 gcgggacggc gttaccagaa actcagaagg ttcgtccaac caaaccgact ctgacggcag
     4861 tttacgagag agatgatagg gtctgcttca gtaagccaga tgctacacaa ttaggcttgt
     4921 acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac
     4981 gatttaggtg acactataga atacacggaa ttaattctag atgctgctag agattttcca
     5041 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     5101 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     5161 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aagcaaaaag
     5221 cagcataact tcgtataatg tatgctatac gaagttattc ccatggtgaa ttgatcccat
     5281 cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc
     5341 taagatctac agctgccttg taagtcattg gtcttaaagg taccgtcgag gaattcggta
     5401 ccccttccgt gtttcagtta gcctccccca tctcccgggc aaacgtgcgc gccaggtcgc
     5461 agatcgtcgg tatggagccg ggggtggtga cgtgggtctg gaccatcccg gaggtaagtt
     5521 gcagcagggc gtcccggcag ccggcgggcg attggtcgta atccaggata aagacgtgca
     5581 tgggacggag gcgtttggcc aagacgtcca aggcccaggc aaacacgttg tacaggtcgc
     5641 cgttgggggc cagcaactcg ggggcccgaa acagggtaaa taacgtgtcc ccgatatggg
     5701 gtcgtgggcc cgcgttgctc tggggctcgg caccctgggg cggcacggcc gtccccgaaa
     5761 gctgtcccca atcctcccgc cacgacccgc cgccctgcag ataccgcacc gtattggcaa
     5821 gcagcccgta aacgcggcga atcgcggcca gcatagccag gtcaagccgc tcgccggggc
     5881 gctggcgttt ggccaggcgg tcgatgtgtc tgtcctccgg aagggccccc aacacgatgt
     5941 ttgtgccggg caaggtcggc gggatgaggg ccacgaacgc cagcacggcc tggggggtca
     6001 tgctgcccat aaggtatcgc gcggccgggt agcacaggag ggcggcgatg ggatggcggt
     6061 cgaagatgag ggtgagggcc gggggcgggg catgtgagct cccagcctcc cccccgatat
     6121 gaggagccag aacggcgtcg gtcacggcat aaggcatgcc cattgttatc tgggcgcttg
     6181 tcattaccac cgccgcgtcc ccggccgata tctcaccctg gtcgaggcgg tgttgtgtgg
     6241 tgtagatgtt cgcgattgtc tcggaagccc ccagcacctg ccagtaagtc atcggctcgg
     6301 gtacgtagac gatatcgtcg cgcgaaccca gggccaccag cagttgcgtg gtggtggttt
     6361 tccccatccc gtgaggaccg tctatataaa cccgcagtag cgtgggcatt ttctgctcca
     6421 ggcggacttc cgtggcttct tgctgccggc gagggcgcaa cgccgtacgt cggttgctat
     6481 ggccgcgaga acgcgcagcc tggtcgaacg cagacgcgtg ttgatggcag gggtacgaag
     6541 ccatggtatt atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct
     6601 agacgttttt taacctcgac taaacacatg taaagcatgt gcaccgaggc cccagatcag
     6661 atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat acgcttgagg
     6721 agagccattt gactctttcc acaactatcc aactcacaac gtggcactgg ggttgtgccg
     6781 cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg tcgccaggtg
     6841 gggggttccg ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca
     6901 gaggaactgc ttccttcacg acattcaaca gaccttgcat tcctttggcg agaggggaaa
     6961 gacccctagg aatgctcgtc aagaagacag ggccaggttt ccgggccctc acattgccaa
     7021 aagacggcaa tatggtggaa aataaccgga attaatgtcg aggtcgacgg tatcgataag
     7081 cttgatatcg aattccgggg ccgctaacaa caacaattgc attcatttta tgtttcaggt
     7141 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacagat gtgatatggc
     7201 tgattatgat catgaacaga ctgtgaggac tgtgagggct gaggggcctg aaatgagcct
     7261 tgagactgtg aatcaatgcc tgtttcatgc cctgagtctt ccatgttctt ctccccacca
     7321 tcttcatttt tatcagcatt ttcctggctg tcatcatcat catcatcact gtttcttagc
     7381 caatctaaaa ctccaactcc catagccaca ttaaacttca ttttttgata cactgacaaa
     7441 ctaaactctt tgtccaatct ctctttccac tccacaattc tgctctgaat actttgagca
     7501 aactcagcca caggtctgta ccaaattaac ataagaagca aagcaatgcc actttgaatt
     7561 atcctctttt ctaacaaaaa ctcactgcgt tccaggcaat gctttaaata atctttgggc
     7621 ctaaaatcta tttgttttac aaatctggcc tgcagtgttt taggcacact gtactcattc
     7681 atggtgacta ttccaggggg aaatatttga gttcttttat ttaggtgttt cttttctaag
     7741 tttaccttaa cactgccatc caaataatcc cttaaattgt ccaggttatt aattccctga
     7801 cctgaaggca aatctctgga ctcccctcca gtgcccttta catcctcaaa aactactaaa
     7861 aactggtcaa tagctactcc tagctcaaag ttcagcctgt ccaagggcaa attaacattt
     7921 aaagctttcc ccccacataa ttcaagcaaa gcagctgcta atgtagtttt accactatca
     7981 attggtcctt taaacagcca gtatcttttt ttaggaatgt tgtacaccat gcattttaaa
     8041 aagtcataca ccactgaatc cattttgggc aacaaacagt gtagccaagc aactccagcc
     8101 atccattctt ctatgtcagc agagcctgta gaaccaaaca ttatatccat cctatccaaa
     8161 agatcattaa atctgtttgt taacatttgt tctctagtta gttgtaggct atcaacccgc
     8221 tttttagcta aaacagtatc aacagcctgt tggcatatgg ttttttggtt tttgctgtca
     8281 gcaaatatag cagcatttgc ataatgcttt tcatggtact tatagtggct gggctgttct
     8341 tttttaatac attttaaaca catttcaaaa ctgtactgaa attccaagta catcccaagc
     8401 aataacaaca catcatcaca ttttgtttcc attgcatact ctgttacaag cttccaggac
     8461 acttgtttag tttcctctgc ttcttctgga ttaaaatcat gctcctttaa cccaccaggc
     8521 aaactttcct caataacaga aaatggatct ctagttaagg cactatacat caaatattcc
     8581 ttattaaccc ctttacaaat taaaaagcta aaggtacaca atttttgagc atagttatta
     8641 atagcagaca ctctatgcct gtgtggagta agaaaaaaca gtatgttatg attataactg
     8701 ttatgcctac ttataaaggt tacagaatat ttttccataa tttttttgta tagcagtgca
     8761 gctttttcct ttgtggtgta aatagcaaag caagcaagag ttctattact aaacacagca
     8821 tgactcaaaa aacttagcaa ttctgaagga aagtccttgg ggtcttctac ctttctcttc
     8881 ttttttggag gagtagaatg ttgagagtca gcagtagcct catcatcact agatggcatt
     8941 tcttctgagc aaaacaggtt ttcctcatta aaggcattcc accactgctc ccattcatca
     9001 gttccatagg ttggaatcta aaatacacaa acaattagaa tcagtagttt aacacattat
     9061 acacttaaaa attttatatt taccttatag ctttaaatct ctgtaggtag tttgtccaat
     9121 tatgtcacac cacagaagta aggttccttc acaaagatca agtccaaacc acattctaaa
     9181 gcaatcgaag cagtagcaat caacccacac aagtggatct ttcctgtata attttctatt
     9241 ttcatgcttc atcctcagta agcacagcaa gcatatgcag ttagtagaca ttttttttgc
     9301 acactcaggc cattgtttgc agtatattgc atcaacacca ggatttaagg aagaagcaaa
     9361 tacctcagtt gcatcccaga agcctccaaa gtcaggttga tgagcatatt ttactccatc
     9421 ttccattttc ttgtacagag tattcatttt cttcattttt tcttcatctc ctcctttatc
     9481 aggatgaaac tccttgcatt tttttaaata tgcctttctc atcagaggaa tattccccca
     9541 ggcactcctt tcaagaccta gaaggtccat tagctgcaaa gattcctctc tgtttaaaac
     9601 tttatccatc tttgcaaagc tttttgcaaa agcctaggcc tccaaaaaag cctcttcact
     9661 acttctggaa tagctcagag gccgggaatt cctgcagccc gggggatcct ctagctagag
     9721 tcggtgtctt ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt
     9781 cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     9841 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     9901 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     9961 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
    10021 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
    10081 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
    10141 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
    10201 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
    10261 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactcccaag cttatcgata
    10321 ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc
    10381 ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa
    10441 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
    10501 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
    10561 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
    10621 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
    10681 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
    10741 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
    10801 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
    10861 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
    10921 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
    10981 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
    11041 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
    11101 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
    11161 atctcctcct ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt
    11221 tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat
    11281 gcacacaata gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct
    11341 gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc
    11401 caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat
    11461 attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc
    11521 gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt
    11581 ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag
    11641 tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc
    11701 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
    11761 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
    11821 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
    11881 cccagtacag gcaaaaagca gctgcttata tgcaggatct gagggctcgc cactccccag
    11941 tcccgcccag gccacgcctc cctggaaagt ccccagcgga aagtcccttg tagcaagctc
    12001 gatatcagca gttcttgaag tactccggat gcagctctcg ggccacgtga tgaaatgcta
    12061 ggcggctgtc aaacctccac tctaacactt ctctctccgg gtcatccatc ccatgcaggc
    12121 tcacagggtg taacaagctg gtgttctctc ctttattggc ctcttctacc ttatctggct
    12181 caactggtac tagcttgtag caccatccaa aggtcagtgg atatctgacc cctggccctg
    12241 gtgtgtagtt ctgctaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga
    12301 tatcttgtct tctttgggag tgaattagcc cttccaacta ctaagtttgt agtacatatt
    12361 taacaaatac aatttcttta aaatgaaaat aattcagagg aatcacaggt ttagagtaaa
    12421 tgaaaccaca ggtaattggc agtggtaata gggtatgggg tgggaagttt gggatgattt
    12481 tggttagctt gagttatcca gttgatccag acatgat
//
