LOCUS       sequence-354506-        9103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing U6 sgRNA cloning plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J 
            Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo
  TITLE     Generation and application of a versatile CRISPR toolkit for 
            mammalian cell engineering
  JOURNAL   Synthetic Biology, Volume 6, Issue 1, 2021
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology, Volume 6, Issue 1, 2021"
COMMENT     Sequence Label: pSB700 lenti gRNA KanR neo
FEATURES             Location/Qualifiers
     source          1..9103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          117..1133
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1057..1079
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1141..1160
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1167..1961
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1167..1961
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1221..1240)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1831..1850
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    1985..2573
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2038..2058)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2456..2467)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2780..3413
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3542..3558)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3542..3558)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3555..3577)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3566..3582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3590..3596,3597..3614,3615..3620))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3635..3656
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3773..3790)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3944..4532)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4024..4043)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4703..5494,5495..5563))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5326..5345
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5564..5668)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5716..5850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5753..5772)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5807..5826
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6018..6651
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6698..6823
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7320..7553
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7738..7782
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8045..8160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        8263..8511
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8263..8283
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8434..8453
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8533..8608
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8682..8985
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gsgcgmgscg grgmggkgcg gsggcrgsca atcagagcgg cgcgctccga aagtttcctt
       61 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      121 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      181 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      241 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
      301 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
      361 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
      421 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
      481 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
      541 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
      601 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
      661 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
      721 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct
      781 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
      841 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
      901 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
      961 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     1021 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1081 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1141 gcaacgtgct ggttattgtg gccaccatga ttgaacaaga tggattgcac gcaggttctc
     1201 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     1261 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     1321 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     1381 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     1441 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     1501 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     1561 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     1621 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     1681 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     1741 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     1801 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     1861 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     1921 agcgcatcgc cttctatcgc cttcttgacg agttcttctg atgtacaagt aaagcgtctg
     1981 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2041 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     2101 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     2161 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     2221 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     2281 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     2341 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     2401 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     2461 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     2521 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     2581 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     2641 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     2701 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     2761 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     2821 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     2881 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     2941 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     3001 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     3061 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     3121 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     3181 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     3241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     3301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     3361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     3421 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     3481 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat
     3541 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3601 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3661 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     3721 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3781 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3841 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3901 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3961 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4021 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4081 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4141 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4201 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4261 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4321 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4381 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4441 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4501 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4561 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4621 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4681 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4741 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4801 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4861 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4921 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4981 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5041 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5101 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5161 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5221 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5281 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5341 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5401 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5461 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5521 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5581 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5641 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     5701 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5761 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5821 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     5881 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
     5941 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     6001 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
     6061 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     6121 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     6181 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     6241 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     6301 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     6361 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     6421 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     6481 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6541 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6601 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     6661 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     6721 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     6781 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     6841 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     6901 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     6961 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     7021 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     7081 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     7141 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
     7201 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     7261 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     7321 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     7381 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     7441 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     7501 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     7561 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     7621 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     7681 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     7741 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     7801 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     7861 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     7921 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     7981 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     8041 cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     8101 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     8161 tcgggtttat tacagggaca gcagagatcc agtttatcat tagtgaacgg atctcgacgg
     8221 tatcgatcac gagactagcc tcgagcggcc gcccccttca ccgagggcct atttcccatg
     8281 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
     8341 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     8401 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     8461 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cggagacgat
     8521 taatgcgtct cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     8581 acttgaaaaa gtggcaccga gtcggtgctt tttttgaatt ctcgacctcg agacaaatgg
     8641 cagtattcgt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     8701 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     8761 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     8821 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     8881 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     8941 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     9001 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     9061 attttttaat tattttgtgc agcgatgggg gcgggggggg ggg
//
