LOCUS       sequence-354507-       11280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of genes with Gal4DBDVP64
            synNotch Receptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11280)
  AUTHORS   Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim 
            WA
  TITLE     Engineering Customized Cell Sensing and Response Behaviors Using 
            Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. 
            Epub 2016 Jan 28.
  PUBMED    26830878
REFERENCE   2  (bases 1 to 11280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.012"; journalName: "Cell"; date: 
            "2016-02-11- 11"; volume: "164"; issue: "4"; pages: "780-91"
COMMENT     Sequence Label: pHR_Gal4UAS_IRES_mC_pGK_tBFP
FEATURES             Location/Qualifiers
     source          1..11280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(161..178)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     388..407
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             586..1296
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(731..749)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     999..1018
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        1381..1868
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1416..1439)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1801..1820
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1889..2590
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    2626..3214
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2679..2699)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3097..3108)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3217..3233)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3218..3234)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3324..3557
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3582..3600)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3583..3600)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3890..3909)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4123..4142)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4242..4264
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4302..4320)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4388..4492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4493..4561,4562..5353)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4711..4730)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5524..6112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6013..6032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6266..6283
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6353..6373)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6358..6687
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6538..6673
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7821..7886
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8016..8036
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8461..8595
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8498..8517)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8552..8571
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8763..9396
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9443..9568
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10065..10298
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10483..10527
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10812..10929
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     11155..11179
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    join(11274..11280,1..567)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
       61 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
      121 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
      181 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
      241 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
      301 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
      361 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
      421 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
      481 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
      541 gttttccttt gaaaaacacg atgataaatg agcgctggcg ggtccatggt gagcaagggc
      601 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      661 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      721 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      781 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      841 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      901 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      961 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     1021 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     1081 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     1141 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     1201 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     1261 cactccaccg gcggcatgga cgagctgtac aagtagggcc gcaactccca cctgcaacat
     1321 gcgtgactga ctgaggccgc gactctagag tcgacctgca cgaggttaac gaattctacc
     1381 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     1441 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     1501 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     1561 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     1621 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     1681 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     1741 gtgggtccgg gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     1801 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     1861 gcctttcgac ctgcagccca agcttaccat gagcgagctg attaaggaga acatgcacat
     1921 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     1981 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     2041 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     2101 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     2161 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
     2221 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     2281 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
     2341 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     2401 cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     2461 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
     2521 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     2581 gcttaattaa tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat
     2641 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2701 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2761 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2821 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     2881 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     2941 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3001 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3061 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3121 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3181 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat
     3241 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     3301 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     3361 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     3421 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     3481 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     3541 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     3601 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     3661 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     3721 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     3781 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     3841 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     3901 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     3961 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     4021 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     4081 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     4141 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4201 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     4261 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     4321 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4381 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4441 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4501 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4561 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4621 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4681 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4741 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4801 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4861 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4921 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4981 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5041 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5101 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5161 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5221 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5281 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5341 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5401 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5461 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5521 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5581 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5641 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5701 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5761 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5821 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5881 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5941 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6001 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6061 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6121 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6181 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6241 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6301 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
     6361 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6421 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     6481 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     6541 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6601 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     6661 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
     6721 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     6781 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     6841 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     6901 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     6961 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
     7021 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
     7081 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
     7141 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
     7201 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
     7261 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
     7321 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
     7381 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
     7441 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
     7501 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
     7561 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
     7621 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
     7681 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
     7741 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     7801 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     7861 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     7921 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     7981 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     8041 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     8101 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     8161 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
     8221 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
     8281 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
     8341 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     8401 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     8461 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8521 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8581 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     8641 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     8701 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     8761 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     8821 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     8881 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     8941 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     9001 gtagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     9061 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     9121 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     9181 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     9241 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9301 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9361 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     9421 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     9481 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     9541 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     9601 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     9661 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     9721 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     9781 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     9841 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     9901 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     9961 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    10021 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    10081 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    10141 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    10201 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
    10261 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
    10321 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
    10381 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
    10441 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
    10501 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
    10561 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
    10621 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
    10681 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
    10741 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcca aatggcagta
    10801 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
    10861 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
    10921 caaaattttc gggtttatta cagggacagc agagatccag tttggatcga taagcttgat
    10981 atcgaattcg gagcactgtc ctccgaacgt cggagcactg tcctccgaac gtcggagcac
    11041 tgtcctccga acgtcggagc actgtcctcc gaacggagca tgtcctccga acgtcggagc
    11101 actgtcctcc gaacgactag ttaggcgtgt acggtgggag gcctatataa gcagagctcg
    11161 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
    11221 acaccgggac cgatccagcc tctcgacatt cgttggatcc ttgacttgcg gcccccctct
//
