LOCUS       sequence-354511-        8306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG426GPD-ccdB
FEATURES             Location/Qualifiers
     source          1..8306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    246..293
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1283..1387
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1388..1456,1457..2248)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1606..1625)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2419..3007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2908..2927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3161..3178
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3295..3316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3331..3336,3337..3354,3355..3361)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3369..3385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3374..3396
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3393..3409
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3393..3409
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3428..3448
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3430..3448
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3473..4116
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     4010..4033
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     4122..4138
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4122..4138
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    4146..4270
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4295..4300,4301..4318,4319..4325)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4379..5038
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4445..4464)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5380..5685
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5564..5583
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5726..5850)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5883..5899)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5884..5900)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      5902..6149
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5918..5936)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6167..6186)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6168..6186)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6196..6213)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6196..6212)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6205..6227)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6353..6808
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6440..6459)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6650..6671
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6939..7742)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7743..7958)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(7982..8001)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8101..8123
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8161..8179)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      join(8220..8306,1..1256)
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga ggtactagac
       61 tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata aacacgcact
      121 atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg tgcgacgtga
      181 acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac
      241 gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
      301 ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac gcaccggact
      361 gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca aaagtatctc
      421 tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct ttcgctcctt
      481 gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta ttactcttta
      541 gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata cgaaaatgta
      601 aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat aattttctga
      661 ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca atccacatcg
      721 gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt
      781 aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa tagacaccaa
      841 agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag agactgcatt
      901 atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc
      961 tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt aaaatagcgc
     1021 tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag
     1081 cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg ttggtaaaat
     1141 agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa
     1201 attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc ttcgttcagg
     1261 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1321 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1381 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1441 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1501 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1561 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     1621 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1681 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1741 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1801 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1861 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1921 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1981 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2041 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2101 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2161 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2221 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2281 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2341 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2401 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2461 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2521 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2581 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2641 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2701 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2761 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2821 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2881 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2941 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3001 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3061 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3121 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3181 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     3241 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     3301 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     3361 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     3421 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctcagtt tatcattatc
     3481 aatactcgcc atttcaaaga atacgtaaat aattaatagt agtgattttc ctaactttat
     3541 ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt acatgcccaa aatagggggc
     3601 gggttacaca gaatatataa catcgtaggt gtctgggtga acagtttatt cctggcatcc
     3661 actaaatata atggagcccg ctttttaagc tggcatccag aaaaaaaaag aatcccagca
     3721 ccaaaatatt gttttcttca ccaaccatca gttcataggt ccattctctt agcgcaacta
     3781 cagagaacag gggcacaaac aggcaaaaaa cgggcacaac ctcaatggag tgatgcaacc
     3841 tgcctggagt aaatgatgac acaaggcaat tgacccacgc atgtatctat ctcattttct
     3901 tacaccttct attaccttct gctctctctg atttggaaaa agctgaaaaa aaaggttgaa
     3961 accagttccc tgaaattatt cccctacttg actaataagt atataaagac ggtaggtatt
     4021 gattgtaatt ctgtaaatct atttcttaaa cttcttaaat tctactttta tagttagtct
     4081 tttttttagt tttaaaacac cagaacttag tttcgacgga ttctagaact agtggatccc
     4141 ccatcacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata
     4201 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
     4261 agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     4321 taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat
     4381 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
     4441 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
     4501 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
     4561 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga
     4621 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     4681 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     4741 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     4801 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     4861 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
     4921 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
     4981 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac
     5041 gcgtggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
     5101 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg
     5161 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
     5221 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
     5281 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
     5341 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc
     5401 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
     5461 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
     5521 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     5581 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     5641 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt
     5701 atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta
     5761 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
     5821 ttctcgttca gctttcttgt acaaagtggt gatgggctgc aggaattcga tatcaagctt
     5881 atcgataccg tcgacctcga gtcatgtaat tagttatgtc acgcttacat tcacgccctc
     5941 cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta
     6001 tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt
     6061 tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg
     6121 ttttgggacg ctcgaaggct ttaatttgcg gccggtaccc aattcgccct atagtgagtc
     6181 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     6241 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     6301 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
     6361 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6421 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6481 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6541 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6601 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6661 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6721 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6781 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     6841 gcggtatttc acaccgcata gggtaataac tgatataatt aaattgaagc tctaatttgt
     6901 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt
     6961 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt
     7021 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca
     7081 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt
     7141 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag
     7201 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca
     7261 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt
     7321 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt
     7381 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg
     7441 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg
     7501 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca
     7561 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg
     7621 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat
     7681 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac
     7741 atgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa gaatactggg
     7801 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
     7861 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aggaaaccga
     7921 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa ggtggtatgg tgcactctca
     7981 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     8041 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     8101 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     8161 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagtatg
     8221 atccaatatc aaaggaaatg atagcattga aggatgagac taatccaatt gaggagtggc
     8281 agcatataga acagctaaag ggtagt
//
