LOCUS       sequence-354575-        8372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-on red-shifted luciferase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Schrevens S, Sanglard D
  TITLE     A novel Candida glabrata doxycycline-inducible system for in 
            vitro/in vivo use.
  JOURNAL   FEMS Yeast Res. 2022 Sep 24;22(1):foac046. doi: 
            10.1093/femsyr/foac046.
  PUBMED    36047937
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2022-09-24"; volume: "22(1):foac046. doi"; pages: " 
            10.1093/femsyr/foac046"
COMMENT     Sequence Label: pSSa30
FEATURES             Location/Qualifiers
     source          1..8372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(940..959)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1059..1081
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1119..1137)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1775..1846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1847..1915,1916..2707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2065..2084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2878..3466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3367..3386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3620..3637
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3754..3775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3790..3795,3796..3813,3814..3820)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3828..3844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3833..3855
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3852..3868
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3852..3868
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3887..3907
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3889..3907
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      3990..4177
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4045..4069)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        4198..4253
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    4204..4222
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    4234..4252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(6093..6112)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6094..6112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6122..6139)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6122..6138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6131..6153)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6279..6734
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6366..6385)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6576..6597
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtcatactaa cccattttta tagaagaagt tgctgcttta gttatcgata acggttctca
       61 tatgaaaatt tcggtgatcc ctgagcaggt ggcggaaaca ttggatgctg agaaccattt
      121 cattgttcgt gaagtgttcg atgtgcacct atccgaccaa ggctttgaac tatctaccag
      181 aagtgtgagc ccctaccgga aggattacat ctcggatgat gactctgatg aagactctgc
      241 ttgctatggc gcattcatcg accaagagct tgtcgggaag attgaactca actcaacatg
      301 gaacgatcta gcctctatcg aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt
      361 cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta agcagacagc tccttggcat
      421 acgattagag acacaaacga acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt
      481 tactctcggc ggcattgacc tcttcacgta taaaactaga cctcaagtct cgaacgaaac
      541 agcgatgtac tggtactggt tctcgggagc acaggatgac gcctaacata tgtgaagtgt
      601 gaagggggag attttcactt tattagattt gtatatatgt ataataaata aataaataag
      661 ttaaataaat aattagataa gggtggtaat tattactatt tacaatcaaa ggtggtcctg
      721 caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca
      781 ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt
      841 tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa agaataaaaa
      901 aaaatgatga attgaaaagg tggtatggtg cactctcagt acaatctgct ctgatgccgc
      961 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     1021 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     1081 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     1141 ataggttaat gtcatgataa taatggtttc ttagcaggtt agactaacat gcaaaaagtt
     1201 tatattcatt tttgtagtta ttcttgtttt cattaggatg attacacact gtggatgtgg
     1261 attaatcgat cattgttaat ttagtgtagc tactaccatt tcaaacaaaa cgtaaattct
     1321 tgcagtcgta ctggatctgt gaatctatta gtatatatga attaaagtag cttgtacata
     1381 ttattctgtt gaatcatatc gcagagcatt ggttgaaatc caaaatataa aaatgtaata
     1441 tcacaaaaaa taatactaat tctaacatta atgggtcaga tttttagtga atacttaaat
     1501 ttataatatc gtctatttaa gctagcaaat ggaacaacat ttaaagtaag aacatcatat
     1561 ctacatgaaa atgtatattt caatctgact aataacgcag agcacatctt tcagtgatgt
     1621 ctgtcacatg atcaaaaaga attgtattta aataattcat aataaaagct taaaaaatta
     1681 caataatgaa aataaagtaa ataatgacat gggtaagagt tccgaaatag aatcttagtg
     1741 tacaaaacaa aaattgcatc attagagatc cccacattca aatatgtatc cgctcatgag
     1801 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1861 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1921 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1981 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2041 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     2101 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     2161 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2221 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2281 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2341 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2401 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2461 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2521 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     2581 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     2641 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     2701 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2761 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3541 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3601 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     3661 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     3721 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     3781 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     3841 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     3901 ctaaagggaa caaaagctgg agctccataa agcacgtggc ctcttatcga gaaagaaatt
     3961 accacgcgta tgacgtccga attcgcgtag cgaatttctt atgatttatg atttttatta
     4021 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa
     4081 aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat
     4141 agcatgaggt cgctcttatt gaccacacct ctactggcat gcgagctcct aatttttgtt
     4201 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgga
     4261 tccaattgta ctttatatat agttgaaaca acagggaaag ggcaggataa agaaagaaat
     4321 tgactgcagt ttcctatgat catatttcat ttttgtaatt aacaatataa tataatacac
     4381 ttataaacgg tttaaaaaca gctttatctc agaaaagaga tccaaaatgg aggatgctaa
     4441 gaacattaag aagggccctg cccctttcta tccattagaa gatggtacag caggtgaaca
     4501 acttcacaaa gctatgaaga gatacgcttt agtcccaggt actattgcct tcactgatgc
     4561 ccatatagag gttgacataa catacgcgga gtacttcgag atgtcagtga gattagcgga
     4621 agccatgaag agatatggct tgaatactaa tcacaggatt gtggtttgct ctgaaaactc
     4681 attgcagttc tttatgccag ttttgggtgc attattcatt ggtgtcgctg ttgctccagc
     4741 aaatgatata tacaacgaaa gagaattatt gaactctatg gggatctctc aaccaactgt
     4801 cgtgtttgtc agcaagaagg gattgcaaaa gattcttaac gttcagaaga aacttcccat
     4861 cattcagaag atcatcatca tggacagcaa aaccgactat caaggcttcc aatcaatgta
     4921 tacctttgtt acatctcacc taccacctgg gtttaacgag tatgactttg taccagagtc
     4981 tttcgataga gataagacca ttgctctgat catgaattcc tctggtagta ctggtctacc
     5041 aaagggtgtt gctctgccac atagagcatt atgtgtcaga tttagccatg ctagagatcc
     5101 catatttggt aatcaaatta ttccagacac tgccattctg tccgtcgttc ctttccacca
     5161 tggttttggg atgttcacaa ccttgggtta cctaatctgt ggttttagag ttatgttgat
     5221 gtatcgtttt gaagaagagt tgttcttgag atcattgcaa gactacaaaa tacagacagc
     5281 cctgttggtc ccaacactgt tctctttctt ggctaagagt accctaatag acaagtacga
     5341 tttgagtaac ctacacgaaa tcgcatctgg aggtgcacca ctttccaaag aggttggtga
     5401 agctgtcgcc aaaagatttc acttacctgg cattagacaa ggttatggcc tgacagagac
     5461 tactagtgct atactgatta cccctgaagg agatgataaa cccggtgcag ttggcaaagt
     5521 ggtcccattc tttgaagcaa aagttgtaga cttagatact ggcaaaactc ttggagtgaa
     5581 tcaacgtggt gagttatgtg tacgtggtcc aatgattatg tccggctacg tcaacaatcc
     5641 agaagcaaca aatgccttaa tagacaaaga tggatggcta cattccggtg atatagccta
     5701 ttgggatgag gacgaacact tcttcatcgt agataggctt aagtctttga tcaaatacaa
     5761 gggttatcaa gttgctcctg ctgaattgga gtccattcta ctacagcatc ctaacatctt
     5821 tgatgctggt gttgcaggtt taccagacga cgatgctggt gaacttccag cggctgtggt
     5881 tgtgttggaa catggtaaaa ccatgactga gaaggaaatc gttgactacg tagcttcaca
     5941 agtaactacc gcaaagaaac taagaggtgg agtagtcttt gtagacgaag ttccaaaggg
     6001 tttgacaggc aaattggatg ccagaaagat tagggaaatc ttgattaaag ctaagaaagg
     6061 tggcaagatt gctgtttaag gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     6121 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6181 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6241 gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     6301 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6361 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6421 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6481 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6541 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6601 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6661 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     6721 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     6781 cgcatagggt aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca
     6841 tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc
     6901 ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag
     6961 tcctcttcca acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata
     7021 ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca
     7081 atcgtaacct tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc
     7141 tttgtcgctc ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc
     7201 cttgcatgac aattctgcta acatcaaaag gcctgtcaaa actagagaat aataaagaaa
     7261 acgatctttt caaaaagaaa aaacctttta gttttccttt gttgttgttg tgggtgtgtg
     7321 ctatttatat tatatagttt actcataata ccataaaata ttcggtttga ttaggttatt
     7381 ttaataagct aatttgtttc taatcgtgta atttatgctg tgtatattaa gtagtgtgtg
     7441 cactgcccaa aaatgtttgt tgtttatagt cggttaaaga gaaaaaagaa aaaaagatcc
     7501 atacacacac gttaattagt tgttcaacgt aatacactca tattttgttc ttatttgctt
     7561 tcggtcgctg ttctcaccaa gatttattgc caacgaaaca attttttttt atatattttc
     7621 agatttttct ttttttcctt tcctttcctt ttctaatttt cactcctggt tttctttctt
     7681 tcttagaaac attatctcga tattaatatt aaaaaaatat aatcattcaa aatggacggt
     7741 ggtatgtttt agtttagctt caattctaat tgattgatta atcagttgat tggtttcaat
     7801 atgacaaatg ggtagggtgg gaaaacttca ttttcaattc agatcaaact tttttgttgt
     7861 cgacataata tttctcgttt gggatgttac tgtcacatta ataatacaca cacatcagct
     7921 tataattttg aaagtaattt atcagatatg ttgtgacgat cagtggaaat ggctaacttc
     7981 aatgtatctg ttcttcccct ttttcaaagt tcacgttttt tgattgattg attgatctgt
     8041 cggcagtggt ttcaaaacca ttcggtgagt aatcctatca atcaatgtta cgacaaaagg
     8101 ctcaatattc aaaattgcaa tgttttatgt tttcctacgt gtacttgtgc aaggcaattg
     8161 attcaacatt gcttttggtg tttgacgagt ttctagtttg gacttgtgtt gttatctggg
     8221 ctatacagat ttcccggctc actatgaatt ttttttttcg acgctcagtg cacacaacta
     8281 taaacaacac aaacacaaac acagcaagaa aaaaaaaaaa cgaacattga attgaaacca
     8341 agccaactga aaaattcctt atttaaatga ct
//
