LOCUS       sequence-354579-        8390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-on red-shifted luciferase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Schrevens S, Sanglard D
  TITLE     A novel Candida glabrata doxycycline-inducible system for in 
            vitro/in vivo use.
  JOURNAL   FEMS Yeast Res. 2022 Sep 24;22(1):foac046. doi: 
            10.1093/femsyr/foac046.
  PUBMED    36047937
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2022-09-24"; volume: "22(1):foac046. doi"; pages: " 
            10.1093/femsyr/foac046"
COMMENT     Sequence Label: pSSa29
FEATURES             Location/Qualifiers
     source          1..8390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(942..961)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1061..1083
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1121..1139)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1777..1848
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1849..1917,1918..2709)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2067..2086)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2880..3468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3369..3388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3622..3639
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3756..3777
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3792..3797,3798..3815,3816..3822)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3830..3846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3835..3857
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3854..3870
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3854..3870
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3889..3909
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3891..3909
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      3981..4168
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4036..4060)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        4189..4244
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    4195..4213
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    4225..4243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(6112..6131)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6113..6131)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6141..6158)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6141..6157)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6150..6172)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6298..6753
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6385..6404)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6595..6616
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tgtcatacta acccattttt atagaagaag ttgctgcttt agttatcgat aacggttctc
       61 atatgaaaat ttcggtgatc cctgagcagg tggcggaaac attggatgct gagaaccatt
      121 tcattgttcg tgaagtgttc gatgtgcacc tatccgacca aggctttgaa ctatctacca
      181 gaagtgtgag cccctaccgg aaggattaca tctcggatga tgactctgat gaagactctg
      241 cttgctatgg cgcattcatc gaccaagagc ttgtcgggaa gattgaactc aactcaacat
      301 ggaacgatct agcctctatc gaacacattg ttgtgtcgca cacgcaccga ggcaaaggag
      361 tcgcgcacag tctcatcgaa tttgcgaaaa agtgggcact aagcagacag ctccttggca
      421 tacgattaga gacacaaacg aacaatgtac ctgcctgcaa tttgtacgca aaatgtggct
      481 ttactctcgg cggcattgac ctcttcacgt ataaaactag acctcaagtc tcgaacgaaa
      541 cagcgatgta ctggtactgg ttctcgggag cacaggatga cgcctaacat atgtgaagtg
      601 tgaaggggga gattttcact ttattagatt tgtatatatg tataataaat aaataaataa
      661 gttaaataaa taattagata agggtggtaa ttattactat ttacaatcaa aggtggtcct
      721 gcaggttttt gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac
      781 actacatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg
      841 ttcggagatt accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa
      901 aaaaaatgat gaattgaaaa ggtggtatgg tgcactctca gtacaatctg ctctgatgcc
      961 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     1021 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1081 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     1141 ttataggtta atgtcatgat aataatggtt tcttagcagg ttagactaac atgcaaaaag
     1201 tttatattca tttttgtagt tattcttgtt ttcattagga tgattacaca ctgtggatgt
     1261 ggattaatcg atcattgtta atttagtgta gctactacca tttcaaacaa aacgtaaatt
     1321 cttgcagtcg tactggatct gtgaatctat tagtatatat gaattaaagt agcttgtaca
     1381 tattattctg ttgaatcata tcgcagagca ttggttgaaa tccaaaatat aaaaatgtaa
     1441 tatcacaaaa aataatacta attctaacat taatgggtca gatttttagt gaatacttaa
     1501 atttataata tcgtctattt aagctagcaa atggaacaac atttaaagta agaacatcat
     1561 atctacatga aaatgtatat ttcaatctga ctaataacgc agagcacatc tttcagtgat
     1621 gtctgtcaca tgatcaaaaa gaattgtatt taaataattc ataataaaag cttaaaaaat
     1681 tacaataatg aaaataaagt aaataatgac atgggtaaga gttccgaaat agaatcttag
     1741 tgtacaaaac aaaaattgca tcattagaga tccccacatt caaatatgta tccgctcatg
     1801 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1861 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1921 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1981 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2041 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2101 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2161 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2221 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2281 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2341 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2401 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2461 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2521 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2581 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2641 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2701 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2761 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2821 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2881 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3001 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3541 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3601 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3661 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3721 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     3781 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     3841 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     3901 cactaaaggg aacaaaagct ggagctccat aaagcacgtg gcctcttatc gagaaagaaa
     3961 ttaccacgcg tatgcgcgta gcgaatttct tatgatttat gatttttatt attaaataag
     4021 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
     4081 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg
     4141 tcgctcttat tgaccacacc tctaccggca tgcgagctcc taatttttgt tgacactcta
     4201 tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtgg atcctatgtt
     4261 tttaacctgg gcggctaaat tgtactttat atatagttga aacaacaggg aaagggcagg
     4321 ataaagaaag aaattgactg cagtttccta tgatcatatt tcatttttgt aattaacaat
     4381 ataatataat acacttataa acggtttaaa aacagcttta tctcagaaaa gagatcggat
     4441 ccaaaatgga ggatgctaag aacattaaga agggccctgc ccctttctat ccattagaag
     4501 atggtacagc aggtgaacaa cttcacaaag ctatgaagag atacgcttta gtcccaggta
     4561 ctattgcctt cactgatgcc catatagagg ttgacataac atacgcggag tacttcgaga
     4621 tgtcagtgag attagcggaa gccatgaaga gatatggctt gaatactaat cacaggattg
     4681 tggtttgctc tgaaaactca ttgcagttct ttatgccagt tttgggtgca ttattcattg
     4741 gtgtcgctgt tgctccagca aatgatatat acaacgaaag agaattattg aactctatgg
     4801 ggatctctca accaactgtc gtgtttgtca gcaagaaggg attgcaaaag attcttaacg
     4861 ttcagaagaa acttcccatc attcagaaga tcatcatcat ggacagcaaa accgactatc
     4921 aaggcttcca atcaatgtat acctttgtta catctcacct accacctggg tttaacgagt
     4981 atgactttgt accagagtct ttcgatagag ataagaccat tgctctgatc atgaattcct
     5041 ctggtagtac tggtctacca aagggtgttg ctctgccaca tagagcatta tgtgtcagat
     5101 ttagccatgc tagagatccc atatttggta atcaaattat tccagacact gccattctgt
     5161 ccgtcgttcc tttccaccat ggttttggga tgttcacaac cttgggttac ctaatctgtg
     5221 gttttagagt tatgttgatg tatcgttttg aagaagagtt gttcttgaga tcattgcaag
     5281 actacaaaat acagacagcc ctgttggtcc caacactgtt ctctttcttg gctaagagta
     5341 ccctaataga caagtacgat ttgagtaacc tacacgaaat cgcatctgga ggtgcaccac
     5401 tttccaaaga ggttggtgaa gctgtcgcca aaagatttca cttacctggc attagacaag
     5461 gttatggcct gacagagact actagtgcta tactgattac ccctgaagga gatgataaac
     5521 ccggtgcagt tggcaaagtg gtcccattct ttgaagcaaa agttgtagac ttagatactg
     5581 gcaaaactct tggagtgaat caacgtggtg agttatgtgt acgtggtcca atgattatgt
     5641 ccggctacgt caacaatcca gaagcaacaa atgccttaat agacaaagat ggatggctac
     5701 attccggtga tatagcctat tgggatgagg acgaacactt cttcatcgta gataggctta
     5761 agtctttgat caaatacaag ggttatcaag ttgctcctgc tgaattggag tccattctac
     5821 tacagcatcc taacatcttt gatgctggtg ttgcaggttt accagacgac gatgctggtg
     5881 aacttccagc ggctgtggtt gtgttggaac atggtaaaac catgactgag aaggaaatcg
     5941 ttgactacgt agcttcacaa gtaactaccg caaagaaact aagaggtgga gtagtctttg
     6001 tagacgaagt tccaaagggt ttgacaggca aattggatgc cagaaagatt agggaaatct
     6061 tgattaaagc taagaaaggt ggcaagattg ctgtttaagg tacccaattc gccctatagt
     6121 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6181 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6241 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
     6301 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6361 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6421 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6481 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6541 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6601 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6661 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6721 cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     6781 tctgtgcggt atttcacacc gcatagggta ataactgata taattaaatt gaagctctaa
     6841 tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc
     6901 atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat
     6961 cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct gtagagacca
     7021 catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct aaacccacac
     7081 cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct ctttgagcaa
     7141 taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt
     7201 ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg cctgtcaaaa
     7261 ctagagaata ataaagaaaa cgatcttttc aaaaagaaaa aaccttttag ttttcctttg
     7321 ttgttgttgt gggtgtgtgc tatttatatt atatagttta ctcataatac cataaaatat
     7381 tcggtttgat taggttattt taataagcta atttgtttct aatcgtgtaa tttatgctgt
     7441 gtatattaag tagtgtgtgc actgcccaaa aatgtttgtt gtttatagtc ggttaaagag
     7501 aaaaaagaaa aaaagatcca tacacacacg ttaattagtt gttcaacgta atacactcat
     7561 attttgttct tatttgcttt cggtcgctgt tctcaccaag atttattgcc aacgaaacaa
     7621 ttttttttta tatattttca gatttttctt tttttccttt cctttccttt tctaattttc
     7681 actcctggtt ttctttcttt cttagaaaca ttatctcgat attaatatta aaaaaatata
     7741 atcattcaaa atggacggtg gtatgtttta gtttagcttc aattctaatt gattgattaa
     7801 tcagttgatt ggtttcaata tgacaaatgg gtagggtggg aaaacttcat tttcaattca
     7861 gatcaaactt ttttgttgtc gacataatat ttctcgtttg ggatgttact gtcacattaa
     7921 taatacacac acatcagctt ataattttga aagtaattta tcagatatgt tgtgacgatc
     7981 agtggaaatg gctaacttca atgtatctgt tcttcccctt tttcaaagtt cacgtttttt
     8041 gattgattga ttgatctgtc ggcagtggtt tcaaaaccat tcggtgagta atcctatcaa
     8101 tcaatgttac gacaaaaggc tcaatattca aaattgcaat gttttatgtt ttcctacgtg
     8161 tacttgtgca aggcaattga ttcaacattg cttttggtgt ttgacgagtt tctagtttgg
     8221 acttgtgttg ttatctgggc tatacagatt tcccggctca ctatgaattt tttttttcga
     8281 cgctcagtgc acacaactat aaacaacaca aacacaaaca cagcaagaaa aaaaaaaaac
     8341 gaacattgaa ttgaaaccaa gccaactgaa aaattcctta tttaaatgac
//
