LOCUS       sequence-354597-       10731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ePiggyBac vector that has a blasticidin selectable cassette and a 
            LoxP exchangeable dual colors cassette with SHH expression (Red 
            module-dsRed/Green module-NeonGreen T2A SHH).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   De Santis R, Etoc F, Rosado-Olivieri EA, Brivanlou AH
  TITLE     Self-organization of human dorsal-ventral forebrain structures by 
            light induced SHH.
  JOURNAL   Nat Commun. 2021 Nov 19;12(1):6768. doi: 10.1038/s41467-021-26881-w.
  PUBMED    34799555
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-11-19"; volume: "12(1):6768. doi"; pages: " 
            10.1038/s41467-021-26881-w"
COMMENT     Sequence Label: ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A...
FEATURES             Location/Qualifiers
     source          1..10731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   2237..2271
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    2559..2680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2722..3120)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(3139..3186)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(3195..4406)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(3339..3358)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     enhancer        4426..4805
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          5085..6101
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6025..6047
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6109..6128
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(6303..6336)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6365..6374
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6371..7048
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(6551..6571)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6769..6788
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6963..6986
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    7178..7299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7331..7364)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7390..7410
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7414..8118
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAATVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     CDS             8119..8172
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    9683..9804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   9990..10052
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10087..10106)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10088..10106)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10116..10133)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10116..10132)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10125..10147)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10274..10729
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10361..10380)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10571..10592
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggggcgc
     2221 gccgcagcta gattaaccct agaaagatag tctgcgtaaa attgacgcat gcattcttga
     2281 aatattgctc tctctttcta aatagcgcga atccgtcgct gtgcatttag gacatctcag
     2341 tcgccgcttg gagctcccgt gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga
     2401 actataacaa ccgcgtgagt caaaatgacg catgattatc ttttacgtga cttttaagat
     2461 ttaactcata cgataattat attgttattt cgtgttctac ttacgtgata acttattata
     2521 tatatatttt cttgttatag atgggaattc agacatgata agatacattg atgagtttgg
     2581 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     2641 tgctttattt gtaaccatta taagctgcaa taaacaagtt tcgaggtcga gtgtcagtcc
     2701 tgctcctcgg ccacgaagtg cttagccctc ccacacataa ccagagggca gcaattcacg
     2761 aatcccaact gccgtcggct gtccatcact gtccttcact atggctttga tcccaggatg
     2821 cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc aagatgcccc tgttctcatt
     2881 tccgatcgcg acgatacaag tcaggttgcc agctgccgca gcagcagcag tgcccagcac
     2941 cacgagttct gcacaaggtc ccccagtaaa atgatataca ttgacaccag tgaagatgcg
     3001 gccgtcgcta gagagagctg cgctggcgac gctgtagtct tcagagatgg ggatgctgtt
     3061 gattgtagcc gttgctcttt caatgagggt ggattcttct tgagacaaag gcttggccat
     3121 ggtttagttc ctcaccttgt cgtattatac tatgccgata tactatgccg atgattaatt
     3181 gtcaacacgg tccggtctaa caaaaaagcc aaaaacggcc agaatttagc ggacaattta
     3241 ctagtctaac actgaaaatt acatattgac ccaaatgatt acatttcaaa aggtgcctaa
     3301 aaaacttcac aaaacacact cgccaacccc gagcgcatag ttcaaaaccg gagcttcagc
     3361 tacttaagaa gataggtaca taaaaccgac caaagaaact gacgcctcac ttatccctcc
     3421 cctcaccaga ggtccggcgc ctgtcgattc aggagagcct accctaggcc cgaaccctgc
     3481 gtcctgcgac ggagaaaagc ctaccgcaca cctaccggca ggtggcccca ccctgcatta
     3541 taagccaaca gaacgggtga cgtcacgaca cgacgagggc gcgcgctccc aaaggtacgg
     3601 gtgcactgcc caacggcacc gccataactg ccgcccccgc aacagacgac aaaccgagtt
     3661 ctccagtcag tgacaaactt cacgtcaggg tccccagatg gtgccccagc ccatctcacc
     3721 cgaataagag ctttcccgca ttagcgaagg cctcaagacc ttgggttctt gccgcccacc
     3781 atgcccccca ccttgtttca acgacctcac agcccgcctc acaagcgtct tccattcaag
     3841 actcgggaac agccgccatt ttgctgcgct ccccccaacc cccagttcag ggcaaccttg
     3901 ctcgcggacc cagactacag cccttggcgg tctctccaca cgcttccgtc ccaccgagcg
     3961 gcccggcggc cacgaaagcc ccggccagcc cagcagcccg ctactcacca agtgacgatc
     4021 acagcgatcc acaaacaaga accgcgaccc aaatcccggc tgcgacggaa ctagctgtgc
     4081 cacacccggc gcgtccttat ataatcatcg gcgttcaccg ccccacggag atccctccgc
     4141 agaatcgccg agaagggact acttttcctc gcctgttccg ctctctggaa agaaaaccag
     4201 tgccctagag tcacccaagt cccgtcctaa aatgtccttc tgctgatact ggggttctaa
     4261 ggccgagtct tatgagcagc gggccgctgt cctgagcgtc cgggcggaag gatcaggacg
     4321 ctcgctgcgc ccttcgtctg acgtggcagc gctcgccgtg aggagggggg cgcccgcggg
     4381 gggcgccaaa acccggcgcg gaggccagat ctcccgatct cgagcgacat tgattattga
     4441 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     4501 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     4561 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     4621 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     4681 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4741 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4801 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
     4861 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
     4921 ggggggggcg cgcgccrggg gggggggggg ggggsggggg gsggggsggg gsgrggsggr
     4981 gaggkgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
     5041 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
     5101 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
     5161 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
     5221 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
     5281 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
     5341 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
     5401 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     5461 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     5521 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
     5581 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     5641 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     5701 gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     5761 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     5821 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     5881 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     5941 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     6001 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     6061 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     6121 ttattgtgct gtctcatcat tttggcaaag aattaaattt aattaatctc gacggtatcg
     6181 gttaacgaat ccaagggcga attccagcac actggcggcc gttactagtg gatcccccgg
     6241 gctgcaggaa ttcggcgcgc cacgcgtacc gatagcttcg aattctgcag tcgaccggaa
     6301 ttataacttc gtatagcata cattatacga agttataatt ctgcagatat ccagcacagt
     6361 ggcggccacc atggatagca ctgagaacgt catcaagccc ttcatgcgct tcaaggtgca
     6421 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggcaagcc
     6481 ctacgagggc acccagaccg ccaagctgca ggtgaccaag ggcggccccc tgcccttcgc
     6541 ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc
     6601 cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     6661 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcac
     6721 cttcatctac cacgtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtaatgca
     6781 gaagaagact ctgggctggg agccctccac cgagcgcctg tacccccgcg acggcgtgct
     6841 gaagggcgag atccacaagg cgctgaagct gaagggcggc ggccactacc tggtggagtt
     6901 caagtcaatc tacatggcca agaagcccgt gaagctgccc ggctactact acgtggactc
     6961 caagctggac atcacctccc acaacgagga ctacaccgtg gtggagcagt acgagcgcgc
     7021 cgaggcccgc caccacctgt tccagtagag ctagctacaa gcttgactct agatcataat
     7081 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     7141 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     7201 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7261 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggaattcgct agctgaataa
     7321 ggccgctcga ataacttcgt atagcataca ttatacgaag ttattcgagt ctagaggacc
     7381 ggtatgggcc caaagaagaa gagaaaggtt tcggtgagca agggcgagga ggataacatg
     7441 gcctctctcc cagcgacaca tgagttacac atctttggct ccatcaacgg tgtggacttt
     7501 gacatggtgg gtcagggcac cggcaatcca aatgatggtt atgaggagtt aaacctgaag
     7561 tccaccaagg gtgacctcca gttctccccc tggattctgg tccctcatat cgggtatggc
     7621 ttccatcagt acctgcccta ccctgacggg atgtcgcctt tccaggccgc cacggtagat
     7681 ggctccggat accaagtcca tcgcacaatg cagtttgaag atggtgcctc ccttactgtt
     7741 aactaccgct acacctacga gggaagccac atcaaaggag aggcccaggt gaaggggact
     7801 ggtttccctg ctgacggtcc tgtgatgacc aactcgctga ccgctgcgga ctggtgcagg
     7861 tcgaagaaga cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc
     7921 actggaaatg gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca
     7981 atggcggcta actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag
     8041 cactccaaga ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc
     8101 atggacgagc tgtacaagga gggcagagga agtcttctaa catgcggtga cgtggaggag
     8161 aatcccggcc caatgctgct gctggcgaga tgtctgctgc tagtcctcgt ctcctcgctg
     8221 ctggtatgct cgggactggc gtgcggaccg ggcagggggt tcgggaagag gaggcacccc
     8281 aaaaagctga cccctttagc ctacaagcag tttatcccca atgtggccga gaagacccta
     8341 ggcgccagcg gaaggtatga agggaagatc tccagaaact ccgagcgatt taaggaactc
     8401 acccccaatt acaaccccga catcatattt aaggatgaag aaaacaccgg agcggacagg
     8461 ctgatgactc agaggtgtaa ggacaagttg aacgctttgg ccatctcggt gatgaaccag
     8521 tggccaggag tgaaactgcg ggtgaccgag ggctgggacg aagatggcca ccactcagag
     8581 gagtctctgc actacgaggg ccgcgcagtg gacatcacca cgtctgaccg cgaccgcagc
     8641 aagtacggca tgctggcccg cctggcggtg gaggccggct tcgactgggt gtactacgag
     8701 tccaaggcac atatccactg ctcggtgaaa gcagagaact cggtggcggc caaatcggga
     8761 ggctgcttcc cgggctcggc cacggtgcac ctggagcagg gcggcaccaa gctggtgaag
     8821 gacctgagcc ccggggaccg cgtgctggcg gcggacgacc agggccggct gctctacagc
     8881 gacttcctca ctttcctgga ccgcgacgac ggcgccaaga aggtcttcta cgtgatcgag
     8941 acgcgggagc cgcgcgagcg cctgctgctc accgccgcgc acctgctctt tgtggcgccg
     9001 cacaacgact cggccaccgg ggagcccgag gcgtcctcgg gctcggggcc gccttccggg
     9061 ggcgcactgg ggcctcgggc gctgttcgcc agccgcgtgc gcccgggcca gcgcgtgtac
     9121 gtggtggccg agcgtgacgg ggaccgccgg ctcctgcccg ccgctgtgca cagcgtgacc
     9181 ctaagcgagg aggccgcggg cgcctacgcg ccgctcacgg cccagggcac cattctcatc
     9241 aaccgggtgc tggcctcgtg ctacgcggtc atcgaggagc acagctgggc gcaccgggcc
     9301 ttcgcgccct tccgcctggc gcacgcgctc ctggctgcac tggcgcccgc gcgcacggac
     9361 cgcggcgggg acagcggcgg cggggaccgc gggggcggcg gcggcagagt agccctaacc
     9421 gctccaggtg ctgccgacgc tccgggtgcg ggggccaccg cgggcatcca ctggtactcg
     9481 cagctgctct accaaatagg cacctggctc ctggacagcg aggccctgca cccgctgggc
     9541 atggcggtca agtccagctg agcggccgcg actctagatc ataatcagcc ataccacatt
     9601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     9661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     9721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9781 gtccaaactc atcaatgtat cttaaggaat tcgataaaag ttttgttact ttatagaaga
     9841 aattttgagt ttttgttttt ttttaataaa taaataaaca taaataaatt gtttgttgaa
     9901 tttattatta gtatgtaagt gtaaatataa taaaacttaa tatctattca aattaataaa
     9961 taaacctcga tatacagacc gataaaacac atgcgtcaat tttacgcatg attatcttta
    10021 acgtacgtca caatatgatt atctttctag ggttaatcta gctgcggcgc gccggtaccc
    10081 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
    10141 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    10201 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    10261 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    10321 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    10381 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    10441 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    10501 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    10561 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    10621 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    10681 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta g
//
