LOCUS       sequence-354599-        9065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ePiggyBac vector to conditionally express using DOX a 
            light-inducible Cre-recombinase enzyme that takes advantage of the 
            Magnets dimerization system (Magnet-CRE).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   De Santis R, Etoc F, Rosado-Olivieri EA, Brivanlou AH
  TITLE     Self-organization of human dorsal-ventral forebrain structures by 
            light induced SHH.
  JOURNAL   Nat Commun. 2021 Nov 19;12(1):6768. doi: 10.1038/s41467-021-26881-w.
  PUBMED    34799555
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-11-19"; volume: "12(1):6768. doi"; pages: " 
            10.1038/s41467-021-26881-w"
COMMENT     Sequence Label: ePB-PURO-TT-PA-CRE
FEATURES             Location/Qualifiers
     source          1..9065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..316
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    46..64
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    224..242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     289..313
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             963..983
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             999..1055
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1062..1115
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1125..1145
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2590..2711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   2897..2959
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2994..3013)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2995..3013)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3023..3040)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3023..3039)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3032..3054)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3181..3636
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3268..3287)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3478..3499
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3662..3766
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3767..3835,3836..4627)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3985..4004)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4798..5386
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5287..5306
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5540..5557
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5674..5695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5710..5715,5716..5733,5734..5740)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5748..5764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5753..5775
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5772..5788
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5772..5788
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5807..5827
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5809..5827
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   5875..5909
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6197..6318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6365..6964)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MAEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6448..6468)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             complement(6968..7021)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(7022..7027,7028..7063,7064..7066,7067..7102,
                     7103..7105,7106..7141,7142..7147,7148..7765))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(7784..7831)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7840..9051)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(7984..8003)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
       61 gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc
      121 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
      181 gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
      241 gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
      301 gaaccgtcag atcgcctgga gaattcgagc tcggtacccg gggatccaag cttaccacca
      361 tggccacctc tgatgaagtc aggaagaacc tgatggacat gttcagggac aggcaggcct
      421 tctctgaaca cacctggaag atgctcctgt ctgtgtgcag atcctgggct gcctggtgca
      481 agctgaacgg tacccatact ctttatgccc ccggtggata tgacattatg ggatatctgg
      541 accagatcgg caaccggcca aacccgcagg tggaactggg ccccgtggat acatcctgcg
      601 ccttgattct ttgtgacctg aaacagaaag acaccccgat agtttacgcg agtgaagcct
      661 tcctctacat gacaggttac agcaacgcag aggtgctggg ccggaattgc cggtttctgc
      721 aaagccctga cggcatggtg aagcccaaga gcacccggaa gtacgtggat agtaacacaa
      781 tcaatactat gcgcaaggca atcgacagga atgccgaggt gcaggttgaa gtagtcaatt
      841 ttaaaaagaa tggacagcga tttgttaatt tcctgactat gatacctgtt agggacgaaa
      901 caggcgagta tcgatactct atgggattcc agtgcgaaac agaaggcgga agcggtggcg
      961 tgcccaagaa gaagaggaaa gtcggatccg gcagcggcgc caccaacttc agcctgctga
     1021 agcaggccgg cgacgtggag gagaaccccg gccccctcga cgagggcaga ggaagtcttc
     1081 taacatgcgg tgacgtggag gagaatcccg gccctgtcga ggtgcccaag aagaagagga
     1141 aagtcggcgg acatactctt tatgcccccg gtggatatga cattatggga tatctgaggc
     1201 agatcaggaa ccggccaaac ccgcaggtgg aactgggccc cgtggataca tcctgcgcct
     1261 tgattctttg tgacctgaaa cagaaagaca ccccgatagt ttacgcgagt gaagccttcc
     1321 tctacatgac aggttacagc aacgcagagg tgctgggccg gaattgccgg tttctgcaaa
     1381 gccctgacgg catggtgaag cccaagagca cccggaagta cgtggatagt aacacaatca
     1441 atactatgcg caaggcaatc gacaggaatg ccgaggtgca ggttgaagta gtcaatttta
     1501 aaaagaatgg acagcgattt gttaatttcc tgactatgat acctgttagg gacgaaacag
     1561 gcgagtatcg atactctatg ggattccagt gcgaaacaga aggtaccaac aggaaatggt
     1621 tccctgctga acctgaggat gtgagggact acctcctgta cctgcaagcc agaggcctgg
     1681 ctgtgaagac catccaacag cacctgggcc agctcaacat gctgcacagg agatctggcc
     1741 tgcctcgccc ttctgactcc aatgctgtgt ccctggtgat gaggagaatc agaaaggaga
     1801 atgtggatgc tggggagaga gccaagcagg ccctggcctt tgaacgcact gactttgacc
     1861 aagtcagatc cctgatggag aactctgaca gatgccagga catcaggaac ctggccttcc
     1921 tgggcattgc ctacaacacc ctgctgcgca ttgccgaaat tgccagaatc agagtgaagg
     1981 acatctcccg caccgatggt gggagaatgc tgatccacat tggcaggacc aagaccctgg
     2041 tgtccacagc tggtgtggag aaggccctgt ccctgggggt taccaagctg gtggagagat
     2101 ggatctctgt gtctggtgtg gctgatgacc ccaacaacta cctgttctgc cgggtcagaa
     2161 agaatggtgt ggctgcccct tctgccacct cccaactgtc cacccgggcc ctggaaggga
     2221 tctttgaggc cacccaccgc ctgatctatg gtgccaagga tgactctggg cagagatacc
     2281 tggcctggtc tggccactct gccagagtgg gtgctgccag ggacatggcc agggctggtg
     2341 tgtccatccc tgaaatcatg caggctggtg gctggaccaa tgtgaacatt gtgatgaact
     2401 acatcagaaa cctggactct gagactgggg ccatggtgag gctgctcgaa gatggggact
     2461 aatctagagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     2521 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     2581 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2641 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2701 aatgtatctt aaggaattcg ataaaagttt tgttacttta tagaagaaat tttgagtttt
     2761 tgtttttttt taataaataa ataaacataa ataaattgtt tgttgaattt attattagta
     2821 tgtaagtgta aatataataa aacttaatat ctattcaaat taataaataa acctcgatat
     2881 acagaccgat aaaacacatg cgtcaatttt acgcatgatt atctttaacg tacgtcacaa
     2941 tatgattatc tttctagggt taatctagct gcggcgcgcc ggtacccaat tcgccctata
     3001 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     3061 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     3121 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     3181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     3421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     3481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     3541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     3601 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     3661 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3721 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3781 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3841 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3901 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3961 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4021 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4081 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4141 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4201 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4261 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4321 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4381 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4441 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4501 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4561 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4621 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4681 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4741 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4801 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4861 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4921 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4981 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5041 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5101 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5161 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5221 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5281 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5341 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5401 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5461 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5521 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     5581 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     5641 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     5701 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     5761 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     5821 ctaaagggaa caaaagctgg agctccaccg cggggcgcgc cgcagctaga ttaaccctag
     5881 aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc tctttctaaa
     5941 tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga gctcccgtga
     6001 ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacaacc gcgtgagtca
     6061 aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg ataattatat
     6121 tgttatttcg tgttctactt acgtgataac ttattatata tatattttct tgttatagat
     6181 gggaattcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     6241 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     6301 agctgcaata aacaagtttc gaggtcgagt gtcagtcctg ctcctcggcc acgaagtgct
     6361 tagctcaggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga
     6421 cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg
     6481 tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg
     6541 cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg
     6601 cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc
     6661 gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga
     6721 cgctctccgg cgtggtccag accgccaccg cggcgccgtc gtccgcgacc cacaccttgc
     6781 cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt
     6841 ggcggtccgg gtcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg
     6901 tgcgtacggc ccgggggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg
     6961 ccatggtagg gccgggattc tcctccacgt caccgcatgt tagaagactt cctctgccct
     7021 ccccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc atatcaaggt
     7081 caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca agggcgtcgg
     7141 ccggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt agttccaggc
     7201 cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct tgtctcagaa
     7261 gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact tgatgctcct
     7321 gttcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg tacagtgcgt
     7381 tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg gtttcgtact
     7441 gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt gagaggagag
     7501 cacagcggaa tgacttggcg ttgttccgca gaaagtcttg ccatgactcg ccttccaggg
     7561 ggcagaagtg ggtatgatgc ctgtccagca tctcgattgg cagggcatcg agcagggccc
     7621 gcttgttctt cacgtgccag tacagggtag gctgctcaac tcccagcttt tgagcgagtt
     7681 tccttgtcgt caggccttcg ataccgactc cattgagtaa ttccagagcg ccgtttatga
     7741 ctttgctctt gtccagtcta gacatggttt agttcctcac cttgtcgtat tatactatgc
     7801 cgatatacta tgccgatgat taattgtcaa cacggtccgg tctaacaaaa aagccaaaaa
     7861 cggccagaat ttagcggaca atttactagt ctaacactga aaattacata ttgacccaaa
     7921 tgattacatt tcaaaaggtg cctaaaaaac ttcacaaaac acactcgcca accccgagcg
     7981 catagttcaa aaccggagct tcagctactt aagaagatag gtacataaaa ccgaccaaag
     8041 aaactgacgc ctcacttatc cctcccctca ccagaggtcc ggcgcctgtc gattcaggag
     8101 agcctaccct aggcccgaac cctgcgtcct gcgacggaga aaagcctacc gcacacctac
     8161 cggcaggtgg ccccaccctg cattataagc caacagaacg ggtgacgtca cgacacgacg
     8221 agggcgcgcg ctcccaaagg tacgggtgca ctgcccaacg gcaccgccat aactgccgcc
     8281 cccgcaacag acgacaaacc gagttctcca gtcagtgaca aacttcacgt cagggtcccc
     8341 agatggtgcc ccagcccatc tcacccgaat aagagctttc ccgcattagc gaaggcctca
     8401 agaccttggg ttcttgccgc ccaccatgcc ccccaccttg tttcaacgac ctcacagccc
     8461 gcctcacaag cgtcttccat tcaagactcg ggaacagccg ccattttgct gcgctccccc
     8521 caacccccag ttcagggcaa ccttgctcgc ggacccagac tacagccctt ggcggtctct
     8581 ccacacgctt ccgtcccacc gagcggcccg gcggccacga aagccccggc cagcccagca
     8641 gcccgctact caccaagtga cgatcacagc gatccacaaa caagaaccgc gacccaaatc
     8701 ccggctgcga cggaactagc tgtgccacac ccggcgcgtc cttatataat catcggcgtt
     8761 caccgcccca cggagatccc tccgcagaat cgccgagaag ggactacttt tcctcgcctg
     8821 ttccgctctc tggaaagaaa accagtgccc tagagtcacc caagtcccgt cctaaaatgt
     8881 ccttctgctg atactggggt tctaaggccg agtcttatga gcagcgggcc gctgtcctga
     8941 gcgtccgggc ggaaggatca ggacgctcgc tgcgcccttc gtctgacgtg gcagcgctcg
     9001 ccgtgaggag gggggcgccc gcggggggcg ccaaaacccg gcgcggaggc cagatctccc
     9061 gatct
//
