LOCUS       sequence-354625-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CA5
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(342..361)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(343..361)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(366..382)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(366..382)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      789..1377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1278..1297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1504..1509,1510..1526,1527..1532)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             join(1580..2602,1..168)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1932..1951)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc
       61 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
      121 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc
      181 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa
      241 ctgtgaatgc gcaaaccaac ccttgatcgg ggaagaacag tatgtcgagc tattttttga
      301 cttactgggg atcaagcctg attgggagaa aataaaatat cccctatagt gagtcgtatt
      361 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
      421 ttgcacaaga taaaaatata tcatcatgcc tcctctagac caaagctggt ctcaaatcgc
      481 tacaacttga tctggggtga gtccgtgggc ttgacagaga actggaagga ggcgctgaac
      541 cgtctcaaga gcctgtttgc caccaatatt actagcaatc gctacaactt gcgctggggt
      601 tagtccgtgg gcttgacaga gaactggtag aaggcgttgg acggtttcaa gagcctgttt
      661 gccaccgtca tgactagcaa ttgccgagac catatctggt cgagccaccc atgaccaaaa
      721 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
      781 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      841 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      901 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
      961 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1021 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1081 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1141 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1201 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1261 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1321 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1381 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1441 tttcctgcgt tatccacaga atcaggggat aacgcaggaa agaacatgaa ttaattctca
     1501 tgtttgacag cttatcatcg attagcttta atgcggtagt ttatcacagt taaattgcta
     1561 acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt
     1621 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga
     1681 tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt
     1741 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc
     1801 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc
     1861 cgtcctgtgg attctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     1921 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     1981 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     2041 gggcgccatc tccttacatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     2101 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgccgac ccatgccctt
     2161 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     2221 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt
     2281 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt
     2341 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
     2401 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct
     2461 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
     2521 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
     2581 acagcttcaa ggatcgctcg cg
//
