LOCUS       sequence-354626-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CA6
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(536..541,542..558,559..564)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             612..1802
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(964..983)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1976..1995)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1977..1995)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2000..2016)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2000..2016)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2424..2603,1..409)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
       61 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      121 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      181 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      241 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      301 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      361 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      421 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      481 gttatccaca gaatcagggg ataacgcagg aaagaacatg aattaattct catgtttgac
      541 agcttatcat cgattagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc
      601 aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg
      661 atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc
      721 attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat
      781 ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc
      841 tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt
      901 ggattctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
      961 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     1021 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     1081 tctccttaca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     1141 gctgcttcct aatgcaggag tcgcataagg gagagcgccg acccatgccc ttgagagcct
     1201 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     1261 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     1321 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     1381 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     1441 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     1501 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     1561 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     1621 aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg
     1681 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
     1741 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
     1801 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca
     1861 attcttgcgg agaactgtga atgcgcaaac caacccttga tcggggaaga acagtatgtc
     1921 gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa atatccccta
     1981 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     2041 tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct agaccaaagc
     2101 tggtctcgga ttgctagtca tgacggtggc aaacaggctc ttgaaaccgt ccaacgcctt
     2161 ctaccagttc tctgtcaagc ccacggacta accccagcgc aagttgtagc gattgctagt
     2221 aatattggtg gcaaacaggc tcttgagacg gttcagcgcc tccttccagt tctctgtcaa
     2281 gcccacggac tcaccccaga tcaagttgta gcgatagcct gagaccatat ctggtcgagc
     2341 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     2401 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2461 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2521 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2581 tagccgtagt taggccacca ctt
//
