LOCUS       sequence-354630-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CA7
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16..32)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16..32)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      440..1028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     929..948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1155..1160,1161..1177,1178..1183)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1231..2421
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1583..1602)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(join(2595..2603,1..11))
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(join(2596..2603,1..11))
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
       61 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac caaagctggt
      121 ctcgagccag tcatgacggt ggcaaacagg ctcttgaaac cgtccaacgc cttctaccag
      181 ttctctgtca agcccacgga ctaaccccag cgcaagttgt agcgattgct agtaatattg
      241 gtggcaaaca ggctcttgag acggttcagc gcctccttcc agttctctgt caagcccacg
      301 gactcacccc agatcaagtt gtagcgattg caagttgaga ccatatctgg tcgagccacc
      361 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
      421 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      481 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      541 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      601 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      661 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      721 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      781 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      841 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      901 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      961 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1021 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1081 ctcacatgtt ctttcctgcg ttatccacag aatcagggga taacgcagga aagaacatga
     1141 attaattctc atgtttgaca gcttatcatc gattagcttt aatgcggtag tttatcacag
     1201 ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc
     1261 ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc
     1321 ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg
     1381 ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt
     1441 ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg
     1501 gcgaccacac ccgtcctgtg gattctctac gccggacgca tcgtggccgg catcaccggc
     1561 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     1621 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     1681 gggggactgt tgggcgccat ctccttacat gcaccattcc ttgcggcggc ggtgctcaac
     1741 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgccga
     1801 cccatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     1861 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     1921 gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg
     1981 tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc
     2041 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
     2101 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
     2161 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
     2221 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcatt
     2281 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
     2341 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
     2401 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
     2461 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttgat
     2521 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
     2581 gaaaataaaa tatcccctat agt
//
