LOCUS       sequence-354653-        9211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 
            (40-831).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Langousis G, Sanchez J, Kempf G, Matthias P
  TITLE     Expression and Crystallization of HDAC6 Tandem Catalytic Domains.
  JOURNAL   Methods Mol Biol. 2023;2589:467-480. doi: 
            10.1007/978-1-0716-2788-4_30.
  PUBMED    36255643
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2023"; volume: "2589:467-480. doi"; pages: " 
            10.1007/978-1-0716-2788-4_30"
COMMENT     Sequence Label: pAC8-TwinStrep-3C-DrHDAC6 (40-831)
FEATURES             Location/Qualifiers
     source          1..9211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      517..972
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(604..623)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     814..835
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(989..1008)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1108..1130
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1168..1186)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1254..1358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1359..1427,1428..2219)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1577..1596)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2390..2978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2879..2898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3132..3149
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_recomb     3541..4368
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             3541..3665
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(3703..4308)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     protein_bind    complement(4372..4405)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4595..4643
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4668..4687
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4721..5398)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     complement(4981..5000)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(5407..5516)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        5571..5662
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5587..5604
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(5680..5685,5686..5709,5710..5745,5746..5769)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             5782..5805
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     misc_recomb     join(8224..9211,1..370)
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(join(8240..9211,1..370))
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
ORIGIN
        1 gggttgaggt tccgtcggca ttggtggagc gggcggcaat tcagacatcg atggtggtgg
       61 tggtggtgga ggcgctggaa tgttaggcac gggagaaggt ggtggcggcg gtgccgccgg
      121 tataatttgt tctggtttag tttgttcgcg cacgattgtg ggcaccggcg caggcgccgc
      181 tggctgcaca acggaaggtc gtctgcttcg aggcagcgct tggggtggtg gcaattcaat
      241 attataattg gaatacaaat cgtaaaaatc tgctataagc attgtaattt cgctatcgtt
      301 taccgtgccg atatttaaca accgctcaat gtaagcaatt gtattgtaaa gagattgtct
      361 caagctcgga tcgatcccgc acgccgataa caagcctttt catttttact acagcattgt
      421 agtggcgaga cacttcgctg tcgtcgcctg atgcggtatt ttctccttac gcatctgtgc
      481 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
      541 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      601 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      661 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      721 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      781 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      841 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
      901 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
      961 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     1021 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     1081 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     1141 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     1201 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1261 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1321 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1381 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1441 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1501 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1561 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1621 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1681 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1741 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1801 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1861 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1921 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1981 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2041 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2101 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2161 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2221 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2281 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2341 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2401 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2461 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2521 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2581 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2641 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2701 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2761 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2821 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2881 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2941 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3001 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3061 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3181 ctctccccgc gcgttggccg attcattaat gcagaacggc tccgcccact attaatgaaa
     3241 ttaaaaattc caattttaaa aaacgcagca agagaaacat ttgtatgaaa gaatgcgtag
     3301 aaggaaagaa aaatgtcgtc gacatgctga acaacaagat taatatgcct ccgtgtataa
     3361 aaaaaatatt gaacgatttg aaagaaaaca atgtaccgcg cggcggtatg tacaggaaga
     3421 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
     3481 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
     3541 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
     3601 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
     3661 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
     3721 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
     3781 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
     3841 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
     3901 ctcctcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
     3961 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
     4021 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
     4081 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
     4141 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
     4201 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
     4261 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
     4321 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tataacttcg
     4381 tatagcatac attatacgaa gttatctggt tgaaacgtac ccgtagtggc tatggcaggg
     4441 cttgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg gggttggggt
     4501 ggggaaaagg aagaaacgcg ggcgtattgg tcccaatggg gtctcggtgg ggtatcgaca
     4561 gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac acccgtgcgt
     4621 tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg
     4681 tgtttcagtt agcctccccc atctcccgct attactcgag tcactgggag ccggagtggc
     4741 gggcctcggc gtgctcgtac tgctccacca cggtgtagtc ctcgttgtgg ttggtgatgt
     4801 ccagcttgga gtccacgtag tggttgccgg gcagctgcac gggcttcttg gccttgtaca
     4861 cggtcttgaa gtcgcaggtg tagtggccgc cgtccttcag cttcagggcg tgggagatct
     4921 cgcccttcag cacgccgtcc tgggggtaca gcttctcggt ggagggctcc cagccggcag
     4981 tcttcttctg cattacgggg ccgtcggcgg ggaagttcac gcccttgaac ttcaccttgt
     5041 agatgaaggt gccgtcctgc agggaggagt cctgctgcac ctccaccacg ccgccgtcct
     5101 cgaagttcat ggagcgctcc caggtgaagc cctcggggaa ggacagcttc atgtagtcgg
     5161 ggatgtcggc ggggtgcttc acgtaggcct tggagccgta ctggaactgg ggggacagga
     5221 tgtcccaggc gaagggcagg gggccgccct tggtcacctg cagcttggcg gtctgggtgc
     5281 cctcgtaggg cttgccctcg ccctcgccct cgatctcgaa gtagtggccg ttcacggagc
     5341 cctccatgcg caccttgaac tgcatgaact ccttgatgac gtcctcggtg ttgtccatgg
     5401 tagagtgatt gtaaataaaa tgtaatttac agtatagtat tttaattaat atacaaatga
     5461 tttgataata attcttattt aactataata tattgtgttg ggttgaatta aaggtccgta
     5521 tactagtatc gattcgcgac ctactccgga atattaatag agttgctgat atcatggaga
     5581 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
     5641 tgtaataaaa aaacctataa atacggatct accatggact ccgcttggtc ccacccccag
     5701 ttcgagaagg gtggtggttc cggtggcgga tccggtggtt ctgcttggag ccaccctcaa
     5761 ttcgagaagt ccgctgtgga cctggaagtg ctgttccaag gtcctggcaa agctgaagag
     5821 gagatgagca atgaactgca aaatctggat gttcaaggaa agagcaaagc gacaggaaca
     5881 ggtcttgtat atgtagacgc cttcactcgc tttcactgcc tctgggatgc cagtcatcca
     5941 gagtgtccag cgagagtgag cacggtaatg gagatgctgg agactgaggg tctgctggga
     6001 cgctgtgtac aagtggaggc tagagctgtg acagaagatg agctgctact cgtgcacacg
     6061 aaagagtatg tagaactgat gaaatccaca cagaacatga cagaggaaga actgaagact
     6121 cttgctgaga aatatgactc ggtttacctg catcctgggt ttttcagctc cgcctgtctg
     6181 tcagtgggat cagttctcca gctggtggat aaggtgatga catcacagct gcgtaatggc
     6241 ttctccatca acaggcctcc aggtcaccat gctcaggcag ataagatgaa cggcttctgt
     6301 atgtttaata atctggccat agcagcacga tatgctcaga aacgacacag ggttcaacga
     6361 gttttaattg tggactggga tgtgcatcac ggacagggaa tccagtatat ttttgaagag
     6421 gatcccagtg tgctgtattt ctcagtgcac agatatgaag acggctcatt ctggccccat
     6481 ctaaaggagt ctgacagcag ttcggttggc tcaggagcag gacaaggcta taatatcaac
     6541 ctgccctgga acaaggtggg gatggaaagt ggagattaca tcacagcttt ccagcagctt
     6601 ctcctgcctg tggcatacga gtttcagcct caactggtgt tagtggccgc tggatttgac
     6661 gctgtgatcg gtgacccaaa gggcgggatg caagtgagtc ctgagtgttt ctccattctg
     6721 acacacatgc tgaaaggtgt tgcacaggga aggcttgtgc tggctttaga gggtgggtat
     6781 aacctgcagt ccacagcgga gggtgtttgc gctagtatga ggtctcttct gggtgacccc
     6841 tgccctcacc tgccctcttc tggcgctccc tgtgaaagtg ccttgaagtc aatctcaaag
     6901 accatctcag acttgtatcc attttggaag agtcttcaaa cttttgaagg tggtcctctg
     6961 tctgaagtct cccccctgcc cgctccagta tgtgcagaag tgaaggtttc ttctccgatc
     7021 actgggctag tttatgacca gagaatgatg cttcatcaca acatgtggga cagtcatcat
     7081 ccagagctcc ctcagagaat ttcacgaatc ttctcccgcc atgaggagtt gcgcctgctg
     7141 tcccgttgtc atcggatacc agctcgccta gcaacagaag aggagctggc gctctgtcac
     7201 agttccaagc atataagcat cattaagagt tcagagcaca tgaaacccag agatctaaac
     7261 cgactggggg acgaatacaa ctccattttc atcagcaatg agagctacac ttgtgccctg
     7321 ctggctgctg gctcgtgctt taattctgca caggccatac tcacaggcca ggtgagaaac
     7381 gctgtggcta tagtccgtcc tccgggtcat catgctgaga aagacacagc ttgtgggttc
     7441 tgtttcttta acacagccgc tttgactgca cgctatgccc agagcatcac gcgtgaatct
     7501 ctgcgcgtcc tcattgtcga ctgggacgtt caccatggaa acggcacaca gcacatcttc
     7561 gaggaggatg acagtgttct gtacatctcc ctgcatcgct acgaagatgg ggcattcttc
     7621 ccaaactcag aggatgctaa ctatgataag gtgggactgg ggaaaggcag aggatacaac
     7681 gtcaacatcc cctggaacgg agggaaaatg ggagacccag aatacatggc ggctttccat
     7741 cacttagtga tgcccatagc aagagagttt gccccagagc tggtgctggt ctctgctggg
     7801 tttgatgcag cacgaggtga tccgttagga gggtttcagg tgaccccgga gggctatgct
     7861 cacctcaccc atcagctaat gagtctggct gcaggaagag tgcttatcat tctggagggt
     7921 ggttataacc tcacttccat ctcggagtcc atgtccatgt gcaccagtat gttgttggga
     7981 gactctccgc cttctctaga ccacctgacc cctctgaaga ccagcgccac tgtcagtatt
     8041 aataatgtcc tgcgtgctca cgcccccttc tggagctctc ttagagtcaa cattccagaa
     8101 tctcttcgtt tgtctctacc ttcacctaaa ccaaaaggca aatgtacacc gggggggaag
     8161 ggtaagaagt ctcctcgcca atcctaaggt acccccggac ggccgcttaa ttaattgatc
     8221 cgggttatta gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt
     8281 gttgtacgta ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga
     8341 aaatcaaatg attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt
     8401 tgtaaaatag gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac
     8461 tatctaatgg attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag
     8521 ctttgtcgat attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat
     8581 tatgcgcttt tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca
     8641 cgttaaataa agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat
     8701 cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca
     8761 cacgacgcct attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt
     8821 ttggaattat ttctgattgc gggcgttttt gggcgggttt caatctaact gtgcccgatt
     8881 ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca
     8941 aatctactaa tggcggcggt ggtggagctg atgataaatc taccatcggt ggaggcgcag
     9001 gcggggctgg cggcggaggc ggaggcggag gtggtggcgg tgatgcagac ggcggtttag
     9061 gctcaaatgt ctctttaggc aacacagtcg gcacctcaac tattgtactg gtttcgggcg
     9121 ccgtttttgg tttgaccggt ctgagacgag tgcgattttt ttcgtttcta atagcttcca
     9181 acaattgttg tctgtcgtct aaaggtgcag c
//
