LOCUS       sequence-354655-        1117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  the patatin-like phosphodiesterase CapV variant CapVQ329R cloned in 
            the L-arabinose inducible vector pBAD28 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Li F, Cao L, Bahre H, Kim SK, Schroeder K, Jonas K, Koonce K, 
            Mekonnen SA, Mohanty S, Bai F, Brauner A, Lee VT, Rohde M, Romling U
  TITLE     Patatin-like phospholipase CapV in Escherichia coli - morphological 
            and physiological effects of one amino acid substitution.
  JOURNAL   NPJ Biofilms Microbiomes. 2022 May 11;8(1):39. doi: 
            10.1038/s41522-022-00294-z.
  PUBMED    35546554
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "NPJ 
            Biofilms Microbiomes."; date: "2022-05-11"; volume: "8(1):39. doi"; 
            pages: " 10.1038/s41522-022-00294-z"
COMMENT     Sequence Label: pCapVQ329R
FEATURES             Location/Qualifiers
     source          1..1117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggcgagctcg ctatattctc tggttatggg gttttcaatg tctgaattgc atgtagtcaa
       61 tgaccatgga gtacggctac tctgcctaaa tggtggtggg gccagaggga tgtttacaat
      121 cagtgtgctt gctgagattg agaggatact tgcctctagg caccctgacc aagaaatcag
      181 aattggcgat tactttgacc tcatcgcagg gacatcgata ggtgggatct tggctctcgg
      241 ccttgctaaa ggcaagagtg ctcgcgagtt ggaatgtgtt ttcttagata aagcgaatga
      301 tatttttcca ccaagatggt ggctattcaa tctccttaaa tcacttgttt ctcccattta
      361 tagtagtaaa ccacttcgac aaacaattga aacgatgatc ggtggtgaga ctacctttaa
      421 tgacctggcc cggagagtaa tggtcccggc agtcaatctt tcaacaggaa aacctcagtt
      481 cttcaagaca ccacataacc cagattttac ccgcgacggt gcacttaaat tgattgatgc
      541 cgcactcgca acctccgctg caccgacgtt ctttgcacca caccattgtg aagaccttgg
      601 ttcttatttt gctgatggag gtctggtcgc caacaacccc agttatatcg gatttttgga
      661 agttttcagg gacatgaagt ctgattttcc tgatgttgct cataaagaca tacacatact
      721 taatatcggt actaccgggg aagagtattc acttagccca aggctgctga gcaaaaaatg
      781 gtggaatggc tactgccgac tttggggcgt aggtaaaaaa ctggtcctta caaccatggc
      841 ggctaaccaa catctccata aaaatatgct cctgcgcgag cttacgctcc atggtgcctc
      901 cgataactac accagtctgg atgaagtgat ccctaatgaa gcagccagcg acatcactct
      961 ggataatgcc acgaagagtt ccattgaaaa cctgcgcgca aggggaagac gactcgctac
     1021 tgtgcagttc gctcagaacc agaagcttaa gagctttttc actacacctg caaaaccctt
     1081 taagcgcgca atcgcgcagg agaaactctg atctaga
//
