LOCUS       sequence-354662-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CA8
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3..22)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4..22)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(27..43)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(27..43)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      448..1036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     937..956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1163..1168,1169..1185,1186..1191)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1239..2429
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1591..1610)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
       61 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
      121 ccaaagctgg tctcaggaag caatcgctac aacttgatct ggggtgagtc cgtgggcttg
      181 acagagaact ggaaggaggc gctgaaccgt ctcaagagcc tgtttgccac caatattact
      241 agcaatcgct acaacttgcg ctggggttag tccgtgggct tgacagagaa ctggtagaag
      301 gcgttggacg gtttcaagag cctgtttgcc accgtcatga cttcgagacc atatctggtc
      361 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
      421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      661 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      721 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      781 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      841 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      901 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      961 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1021 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1081 gccttttgct cacatgttct ttcctgcgtt atccacagaa tcaggggata acgcaggaaa
     1141 gaacatgaat taattctcat gtttgacagc ttatcatcga ttagctttaa tgcggtagtt
     1201 tatcacagtt aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca
     1261 tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac
     1321 tgccgggcct cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc
     1381 tgctagcgct atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg
     1441 accgctttgg ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg
     1501 cgatcatggc gaccacaccc gtcctgtgga ttctctacgc cggacgcatc gtggccggca
     1561 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     1621 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     1681 ccgtggccgg gggactgttg ggcgccatct ccttacatgc accattcctt gcggcggcgg
     1741 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     1801 agcgccgacc catgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
     1861 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
     1921 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
     1981 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
     2041 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
     2101 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
     2161 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
     2221 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt
     2281 cgatcattgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg
     2341 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg
     2401 gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt
     2461 caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa
     2521 cccttgatcg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct
     2581 gattgggaga aaataaaata
//
