LOCUS       sequence-354675-        9412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP 
            tag, and c-terminal bi-partite NLS and 6x-His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Christie KA, Guo JA, Silverstein RA, Doll RM, Mabuchi M, Stutzman 
            HE, Lin J, Ma L, Walton RT, Pinello L, Robb GB, Kleinstiver BP
  TITLE     Precise DNA cleavage using CRISPR-SpRYgests.
  JOURNAL   Nat Biotechnol. 2022 Oct 6. pii: 10.1038/s41587-022-01492-y. doi: 
            10.1038/s41587-022-01492-y.
  PUBMED    36203014
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Oct 6. pii: 10.1038/s41587-022-01492-y. doi: 
            10.1038/s41587-022-01492-y."
COMMENT     Sequence Label: pET28-SpRY-NLS-6xHis (gbr2101)
FEATURES             Location/Qualifiers
     source          1..9412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     559..578
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(598..675)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     881..900
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     984..1003
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        984..1002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1003..1027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1042..1064
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5240..5260
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5261..5278
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5330..5348)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5344..5391
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5428..5883
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5515..5534)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5725..5746
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5975..6790)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5975..6790)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6698..6717
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6782..6801
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6912..7500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7401..7420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7654..7671
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7686..7825
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7751..7770)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7911..7933
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7927..8118)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(8927..9412,1..597))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
       61 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
      121 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
      181 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
      241 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
      301 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
      361 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
      421 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
      481 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
      541 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
      601 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
      661 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc
      721 agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg
      781 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
      841 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
      901 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
      961 tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
     1021 caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatataca tgaaaatcga
     1081 agaagacaag aagtactcga tcggattgga cattggaacc aattcagtcg gctgggccgt
     1141 tattaccgac gagtataaag ttccttccaa gaagtttaag gtgcttggaa atacagaccg
     1201 ccacagtatt aagaagaatt tgatcggtgc gcttcttttc gacagcggcg agaccgctga
     1261 gcgtactcgt ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttg
     1321 ttatctacag gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg
     1381 acttgaagag tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg
     1441 aaatatagta gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa
     1501 aaaattggta gattctactg ataaagcgga tttgcgctta atctatttgg ccttagcgca
     1561 tatgattaag tttcgtggtc attttttgat tgagggagat ttaaatcctg ataatagtga
     1621 tgtggacaaa ctatttatcc agttggtaca aacctacaat caattatttg aagaaaaccc
     1681 tattaacgca agtggagtag atgctaaagc gattctttct gcacgattga gtaaatcaag
     1741 acgattagaa aatctcattg ctcagctccc cggtgagaag aaaaatggct tatttgggaa
     1801 tctcattgct ttgtcattgg gtttgacccc taattttaaa tcaaattttg atttggcaga
     1861 agatgctaaa ttacagcttt caaaagatac ttacgatgat gatttagata atttattggc
     1921 gcaaattgga gatcaatatg ctgatttgtt tttggcagct aagaatttat cagatgctat
     1981 tttactttca gatatcctaa gagtaaatac tgaaataact aaggctcccc tatcagcttc
     2041 aatgattaaa cgctacgatg aacatcatca agacttgact cttttaaaag ctttagttcg
     2101 acaacaactt ccagaaaagt ataaagaaat cttttttgat caatcaaaaa acggatatgc
     2161 aggttatatt gatgggggag ctagccaaga agaattttat aaatttatca aaccaatttt
     2221 agaaaaaatg gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg
     2281 caagcaacgg acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca
     2341 tgctattttg agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat
     2401 tgaaaaaatc ttgacttttc gaattcctta ttatgttggt ccattggcgc gtggcaatag
     2461 tcgttttgca tggatgactc ggaagtctga agaaacaatt accccatgga attttgaaga
     2521 agttgtcgat aaaggtgctt cagctcaatc atttattgaa cgcatgacaa actttgataa
     2581 aaatcttcca aatgaaaaag tactaccaaa acatagtttg ctttatgagt attttacggt
     2641 ttataacgaa ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac cagcatttct
     2701 ttcaggtgaa cagaagaaag ccattgttga tttactcttc aaaacaaatc gaaaagtaac
     2761 cgttaagcaa ttaaaagaag attatttcaa aaaaatagaa tgttttgata gtgttgaaat
     2821 ttcaggagtt gaagatagat ttaatgcttc attaggtacc taccatgatt tgctaaaaat
     2881 tattaaagat aaagattttt tggataatga agaaaatgaa gatatcttag aggatattgt
     2941 tttaacattg accttatttg aagataggga gatgattgag gaaagactta aaacatatgc
     3001 tcacctcttt gatgataagg tgatgaaaca gcttaaacgt cgccgttata ctggttgggg
     3061 acgtttgtct cgaaaattga ttaatggtat tagggataag caatctggca aaacaatatt
     3121 agattttttg aaatcagatg gttttgccaa tcgcaatttt atgcagctga tccatgatga
     3181 tagtttgaca tttaaagaag acattcaaaa agcacaagtg tctggacaag gcgatagttt
     3241 acatgaacat attgcaaatt tagctggtag ccctgctatt aaaaaaggta ttttacagac
     3301 tgtaaaagtt gttgatgaat tggtcaaagt aatggggcgg cataagccag aaaatatcgt
     3361 tattgaaatg gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg
     3421 tatgaaacga atcgaagaag gtatcaaaga attaggaagt cagattctta aagagcatcc
     3481 tgttgaaaat actcaattgc aaaatgaaaa gctctatctc tattatctcc aaaatggaag
     3541 agacatgtat gtggaccaag aattagatat taatcgttta agtgattatg atgtcgatca
     3601 cattgttcca caaagtttcc ttaaagacga ttcaatagac aataaggtct taacgcgttc
     3661 tgataaaaat cgtggtaaat cggataacgt tccaagtgaa gaagtagtca aaaagatgaa
     3721 aaactattgg agacaacttc taaacgccaa gttaatcact caacgtaagt ttgataattt
     3781 aacgaaagct gaacgtggag gtttgagtga acttgataaa gctggtttta tcaaacgcca
     3841 attggttgaa actcgccaaa tcactaagca tgtggcacaa attttggata gtcgcatgaa
     3901 tactaaatac gatgaaaatg ataaacttat tcgagaggtt aaagtgatta ccttaaaatc
     3961 taaattagtt tctgacttcc gaaaagattt ccaattctat aaagtacgtg agattaacaa
     4021 ttaccatcat gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa
     4081 atatccaaaa cttgaatcgg agtttgtcta tggtgattat aaagtttatg atgttcgtaa
     4141 aatgattgct aagtctgagc aagaaatagg caaagcaacc gcaaaatatt tcttttactc
     4201 taatatcatg aacttcttca aaacagaaat tacacttgca aatggagaga ttcgcaaacg
     4261 ccctctaatc gaaactaatg gggaaactgg agaaattgtc tgggataaag ggcgagattt
     4321 tgccacagtg cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt
     4381 acagacaggc ggattctcca aggagtcaat tcgccctaag cgcaactcag ataagcttat
     4441 tgcgcgtaag aaagactggg atcccaagaa gtacggcggg ttcctctggc ccacggtggc
     4501 gtacagcgtt cttgttgttg caaaggtaga gaagggcaag agtaagaagc tgaagtccgt
     4561 aaaggagctg cttggcatca ccatcatgga acgctcttct tttgagaaga accctatcga
     4621 cttcttagaa gctaagggct ataaggaagt taagaaagac ctgatcatca agcttcccaa
     4681 gtattcttta ttcgagttgg agaatggccg caagcgtatg ctggcgtcgg caaagcaact
     4741 gcagaagggt aatgagttgg ccctgccgag caagtacgtt aattttctgt atttagcgag
     4801 tcactatgag aagttaaaag gaagccccga ggacaatgag cagaaacaac tgttcgtcga
     4861 gcaacataag cactacttgg atgagattat tgagcagatt agcgagttct caaagcgtgt
     4921 aatcttagca gatgctaact tggataaggt tctgagcgcc tacaacaagc atcgcgataa
     4981 gcctatccgc gaacaggcgg agaatatcat tcacctgttc actctcacac gtttgggcgc
     5041 accccgtgcc tttaaatatt tcgacaccac gatcgaccct aagcaatatc gcagtaccaa
     5101 agaggtgctg gatgccaccc ttatccatca aagcatcacc gggttgtatg agacccgtat
     5161 cgatcttagc cagttaggcg gggacgggtc gggcggcggc ggctcgggca agcgtacagc
     5221 cgacggaagt gagttcgagc ctaaaaagaa gcgtaaggta catcaccatc accatcatta
     5281 aatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     5341 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
     5401 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     5461 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5521 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5581 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5641 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5701 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5761 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5821 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5881 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5941 acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat
     6001 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     6061 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     6121 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     6181 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
     6241 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     6301 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     6361 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     6421 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     6481 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     6541 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     6601 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     6661 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     6721 ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa
     6781 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     6841 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6901 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6961 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7021 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7081 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7141 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7201 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7261 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7321 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7381 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7441 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7501 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7561 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7621 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7681 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
     7741 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     7801 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     7861 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     7921 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
     7981 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
     8041 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
     8101 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
     8161 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
     8221 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
     8281 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag
     8341 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
     8401 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
     8461 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
     8521 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
     8581 ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg
     8641 accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc
     8701 gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg
     8761 cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat
     8821 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
     8881 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
     8941 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9001 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
     9061 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
     9121 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
     9181 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa
     9241 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
     9301 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     9361 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag cc
//
