LOCUS       sequence-354680-       12839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal4 Response element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12839)
  AUTHORS   Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell
            DL, Peet DJ, Whitelaw ML, Bersten DC
  TITLE     dFLASH; dual FLuorescent transcription factor Activity Sensor for 
            Histone integrated live-cell reporting and high-content screening
  JOURNAL   bioRxiv 2023.11.21.568191
REFERENCE   2  (bases 1 to 12839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.11.21.568191"
COMMENT     Sequence Label: pLV-REPORT(PGK/CMV)-GRE
FEATURES             Location/Qualifiers
     source          1..12839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2420..2444
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2549..3250
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2682..2700)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2950..2969
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3144..3167
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3260..3280
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3284..3304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3308..3328
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3377..4504
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             4505..4558
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4553..5353
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4613..4632)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5223..5242
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5398..5421)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5748..5767
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(5870..5889)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6061..6440
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6441..6644
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6595..6615
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6689..6708
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6689..6707
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6737..6761
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6788..6797
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6809..6829
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6833..6853
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6857..6877
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(6887..6889,6890..6892,6893..7603,7604..7609,
                     7610..7729)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(6932..6953)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7193..7212)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             7730..7789
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             7790..8815
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7790..8815
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             10048..10281
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10353..10474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10390..10409)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10444..10463
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10576..10595
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(10860..10878)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10946..11050
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11051..11119,11120..11911)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11269..11288)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12082..12670
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12571..12590
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcgcgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgatg gtaccagctt gcatgcctgc
     2281 aggtcggagt actgtcctcc gagcctccga gcggagtact gtcctccgan cggagtactg
     2341 tcctccgacg gagtactgtc ctccgagcgg agacggcgcg ccagctttag gcgtgtacgg
     2401 tgggcgccta taaaagcaga gctcgtttag tgaaccgtca gatcgcctgg agcaattcca
     2461 caacactttt gtcttatacc aactttccgt accacttcct accctcgtaa agtcgacacc
     2521 ggggcccaga tctatcgatc ggccggatat ggtgagcaag ggcgaggagg tcatcaaaga
     2581 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     2641 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     2701 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     2761 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     2821 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     2881 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     2941 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     3001 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     3061 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     3121 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     3181 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga
     3241 gctgtacaag gccgtggatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa
     3301 ggtagatcca aaaaagaaga gaaaggtaca cggcgccacg aagcaagcag gagatgttga
     3361 agaaaacccc gggcctatgg cttcgtaccc cggccatcaa cacgcgtctg cgttcgacca
     3421 ggctgcgcgt tctcgcggcc atagcaaccg acgtacggcg ttgcgccctc gccggcagca
     3481 agaagccacg gaagtccgcc cggagcagaa aatgcccacg ctactgcggg tttatataga
     3541 cggtccccac gggatgggga aaaccaccac cacgcaactg ctggtggccc tgggttcgcg
     3601 cgacgatatc gtctacgtac ccgagccgat gacttactgg cgggtgctgg gggcttccga
     3661 gacaatcgcg aacatctaca ccacacaaca ccgcctcgac cagggtgaga tatcggccgg
     3721 ggacgcggcg gtggtaatga caagcgccca gataacaatg ggcatgcctt atgccgtgac
     3781 cgacgccgtt ctggctcctc atatcggggg ggaggctggg agctcacatg ccccgccccc
     3841 ggccctcacc ctcatcttcg accgccatcc catcgccgcc ctcctgtgct acccggccgc
     3901 gcggtacctt atgggcagca tgacccccca ggccgtgctg gcgttcgtgg ccctcatccc
     3961 gccgaccttg cccggcacca acatcgtgct tggggccctt ccggaggaca gacacatcga
     4021 ccgcctggcc aaacgccagc gccccggcga gcggctggac ctggctatgc tggctgcgat
     4081 tcgccgcgtt tacgggctac ttgccaatac ggtgcggtat ctgcagtgcg gcgggtcgtg
     4141 gcgggaggac tggggacagc tttcggggac ggccgtgccg ccccagggtg ccgagcccca
     4201 gagcaacgcg ggcccacgac cccatatcgg ggacacgtta tttaccctgt ttcgggcccc
     4261 cgagttgctg gcccccaacg gcgacctgta taacgtgttt gcctgggcct tggacgtctt
     4321 ggccaaacgc ctccgttcca tgcacgtctt tatcctggat tacgaccaat cgcccgccgg
     4381 ctgccgggac gccctgctgc aacttacctc cgggatggtc cagacccacg tcaccacccc
     4441 cggctccata ccgacgatat gcgacctggc gcgcacgttt gcccgggaga tgggggaggc
     4501 taacgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat
     4561 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     4621 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     4681 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     4741 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4801 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4861 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4921 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4981 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     5041 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     5101 cgatgatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     5161 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     5221 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     5281 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     5341 cgagttcttc tgaaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     5401 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     5461 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     5521 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     5581 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     5641 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     5701 tgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     5761 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     5821 catctcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     5881 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     5941 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     6001 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     6061 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     6121 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     6181 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     6241 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     6301 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     6361 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     6421 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     6481 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     6541 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     6601 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
     6661 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
     6721 gctagttaag ctatcaacaa gtttgtacaa aaaagcaggc tttaaaggaa ccaattcagt
     6781 cgaggtcgcc accatggtgc acgtggatcc aaaaaagaag agaaaggtag atccaaaaaa
     6841 gaagagaaag gtagatccaa aaaagaagag aaaggtacac gtgtccatgg tgagcaaggg
     6901 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     6961 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     7021 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     7081 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     7141 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     7201 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     7261 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     7321 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     7381 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     7441 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     7501 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     7561 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaagctta gccatggctt
     7621 cccgccggag gtggaggagc aggatgatgg cacgctgccc atgtcttgtg cccaggagag
     7681 cgggatggac cgtcaccctg cagcctgtgc ttctgctagg atcaatgtgc agtgtactaa
     7741 ttatgctctc ttgaaattgg ctggagatgt tgagagcaac ccaggtccca tgaaaaagcc
     7801 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     7861 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     7921 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     7981 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     8041 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     8101 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     8161 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     8221 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     8281 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     8341 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     8401 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     8461 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     8521 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     8581 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     8641 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     8701 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     8761 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aataggctag
     8821 cctagtgcca tttgttcagt ggttcgtagg gctttccccc actgtttggc tttcagttat
     8881 atggatgatg tggtattggg ggccaagtct gtacagcatc ttgagtccct ttttaccgct
     8941 gttaccaatt ttcttttgtc tttgggtata catttaaacc ctaacaaaac aaagagatgg
     9001 ggttactctc taaattttat gggttatgtc attggatgtt atgggtcctt gccacaagaa
     9061 cacatcatac aaaaaatcaa agaatgtttt agaaaacttc ctattaacag gcctattgat
     9121 tggaaagtat gtcaacgaat tgtgggtctt ttgggttttg ctgccccttt tacacaatgt
     9181 ggttatcctg cgttgatgcc tttgtatgca tgtattcaat ctaagcaggc tttcactttc
     9241 tcgccaactt acaaggcctt tctgtgtaaa caatacctga acctttaccc cgttgcccgg
     9301 caacggccag gtctgtgcca agtgtttgct gacgcaaccc ccactggctg gggcttggtc
     9361 atgggccatc agcgcatgcg tggaaccttt tcggctcctc tgccgatcca tactgcggaa
     9421 ctcctagccg cttgttttgc tcgcagcagg tctggagcaa acattatcgg gactgataac
     9481 tctgttgtcc tatcccgcaa atatacatcg tttccatggc tgctaggctg tgctgccaac
     9541 tggatcctgc gcgggacgtc ctttgtttac gtcccgtcgg cgctgaatcc tgcggacgac
     9601 ccttctcggg gtcgcttggg actctctcgt ccccttctcc gtctgccgtt ccgaccgacc
     9661 acggggcgca cctctcttta cgcggactcc ccgtctgtgc cttctcatct gccggaccgt
     9721 gtgcacttcg cttcacctct gcacgtcgca tggagaccac cgtgaacgcc caccaaatat
     9781 tgcccaaggt cttacataag aggactcttg gactctcagc aatgtcaacg accgaccttg
     9841 aggcatactt caaagactgt ttgtttaaag actgggagga gttgggggag gagattaggt
     9901 taaaggtctt tgtactagga ggctgtaggc ataaattggt ctgcgcacca gcaccatggc
     9961 gcaatcacta gagcggggta cctttaagac caatgactta caaggcagct gtagatctta
    10021 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
    10081 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    10141 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    10201 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    10261 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
    10321 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
    10381 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    10441 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
    10501 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    10561 ctgactaatt ttttttattt atgcagaggc cgaggccgga tcccttgagt ggctttcatc
    10621 ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt
    10681 ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta
    10741 cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc
    10801 aagagggcat tagcaatagt gtttataagg cccccttgtt aattcttgaa gacgaaaggg
    10861 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
    10921 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
    10981 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
    11041 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
    11101 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
    11161 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
    11221 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
    11281 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
    11341 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
    11401 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
    11461 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
    11521 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
    11581 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
    11641 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    11701 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    11761 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    11821 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    11881 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    11941 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    12001 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    12061 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    12121 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    12181 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
    12241 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    12301 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    12361 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    12421 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    12481 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    12541 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    12601 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    12661 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg
    12721 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
    12781 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcg
//
