LOCUS       sequence-354681-        9744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mnucTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9744)
  AUTHORS   Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell
            DL, Peet DJ, Whitelaw ML, Bersten DC
  TITLE     dFLASH; dual FLuorescent transcription factor Activity Sensor for 
            Histone integrated live-cell reporting and high-content screening
  JOURNAL   bioRxiv 2023.11.21.568191
REFERENCE   2  (bases 1 to 9744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.11.21.568191"
COMMENT     Sequence Label: pLV-REPORT(EF1a no GFP)
FEATURES             Location/Qualifiers
     source          1..9744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2325..2349
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2454..3155
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2587..2605)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2855..2874
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3049..3072
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3165..3185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3189..3209
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3213..3233
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        3424..4610
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3655..4601
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4558..4578
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4695..5720
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MQKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4695..5720
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MQKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             6953..7186
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7258..7379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7295..7314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7349..7368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7481..7500
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7765..7783)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7851..7955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7956..8024,8025..8816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8174..8193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8987..9575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9476..9495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgatt tcgaacctag ggtttaaacg
     2281 gcgcgccagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg tttagtgaac
     2341 cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
     2401 ttcctaccct cgtaaagtcg acaccggggc ccagatctat cgatcggccg gatatggtga
     2461 gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca
     2521 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     2581 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     2641 ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt
     2701 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     2761 gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg
     2821 tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg
     2881 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     2941 accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca
     3001 tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca
     3061 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc
     3121 acctgttcct gtacggcatg gacgagctgt acaaggccgt ggatccaaaa aagaagagaa
     3181 aggtagatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtacacggct
     3241 gacgcccgcc ccacgacccg cagcgcccgc cccacgaccc gcagcgcccg accgaaagga
     3301 gcgaccgtcg acctcgacgg atcgggagat ctcgaccgag ctttgcaaag atggataaag
     3361 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga
     3421 attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     3481 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     3541 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     3601 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     3661 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     3721 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     3781 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     3841 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     3901 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     3961 tggcaagata gtcttgtaaa tgcgggccaa gatcgatctg cacactggta tttcggtttt
     4021 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     4081 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     4141 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     4201 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     4261 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     4321 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     4381 cctcgattag ttctcgatcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     4441 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     4501 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     4561 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaattagct
     4621 tgaccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt gctggaattc
     4681 tcgaggtcgc caccatgcaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     4741 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     4801 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     4861 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     4921 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     4981 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     5041 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     5101 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     5161 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     5221 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     5281 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     5341 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     5401 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     5461 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     5521 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     5581 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     5641 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     5701 gtccgagggc aaaggaatag gctagcctag tgccatttgt tcagtggttc gtagggcttt
     5761 cccccactgt ttggctttca gttatatgga tgatgtggta ttgggggcca agtctgtaca
     5821 gcatcttgag tcccttttta ccgctgttac caattttctt ttgtctttgg gtatacattt
     5881 aaaccctaac aaaacaaaga gatggggtta ctctctaaat tttatgggtt atgtcattgg
     5941 atgttatggg tccttgccac aagaacacat catacaaaaa atcaaagaat gttttagaaa
     6001 acttcctatt aacaggccta ttgattggaa agtatgtcaa cgaattgtgg gtcttttggg
     6061 ttttgctgcc ccttttacac aatgtggtta tcctgcgttg atgcctttgt atgcatgtat
     6121 tcaatctaag caggctttca ctttctcgcc aacttacaag gcctttctgt gtaaacaata
     6181 cctgaacctt taccccgttg cccggcaacg gccaggtctg tgccaagtgt ttgctgacgc
     6241 aacccccact ggctggggct tggtcatggg ccatcagcgc atgcgtggaa ccttttcggc
     6301 tcctctgccg atccatactg cggaactcct agccgcttgt tttgctcgca gcaggtctgg
     6361 agcaaacatt atcgggactg ataactctgt tgtcctatcc cgcaaatata catcgtttcc
     6421 atggctgcta ggctgtgctg ccaactggat cctgcgcggg acgtcctttg tttacgtccc
     6481 gtcggcgctg aatcctgcgg acgacccttc tcggggtcgc ttgggactct ctcgtcccct
     6541 tctccgtctg ccgttccgac cgaccacggg gcgcacctct ctttacgcgg actccccgtc
     6601 tgtgccttct catctgccgg accgtgtgca cttcgcttca cctctgcacg tcgcatggag
     6661 accaccgtga acgcccacca aatattgccc aaggtcttac ataagaggac tcttggactc
     6721 tcagcaatgt caacgaccga ccttgaggca tacttcaaag actgtttgtt taaagactgg
     6781 gaggagttgg gggaggagat taggttaaag gtctttgtac taggaggctg taggcataaa
     6841 ttggtctgcg caccagcacc atggcgcaat cactagagcg gggtaccttt aagaccaatg
     6901 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     6961 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     7021 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7081 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7141 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7201 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     7261 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7321 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7381 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7441 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7501 ccggatccct tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
     7561 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
     7621 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
     7681 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
     7741 ttgttaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     7801 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7861 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7921 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7981 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8041 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8101 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8161 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     8221 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8281 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8341 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8401 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8461 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     8521 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8581 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8641 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8701 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8761 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8821 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8881 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8941 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9001 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9061 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9121 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9601 ggttcctggc cttttgctgg ccttgaagct gtccctgatg gtcgtcatct acctgcctgg
     9661 acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg
     9721 ggaaggccat ccagcctcgc gtcg
//
