LOCUS       sequence-354682-       12806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hypoxic responsive reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12806)
  AUTHORS   Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell
            DL, Peet DJ, Whitelaw ML, Bersten DC
  TITLE     dFLASH; dual FLuorescent transcription factor Activity Sensor for 
            Histone integrated live-cell reporting and high-content screening
  JOURNAL   bioRxiv 2023.11.21.568191
REFERENCE   2  (bases 1 to 12806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.11.21.568191"
COMMENT     Sequence Label: pLV-REPORT(PGK)-12xHRE
FEATURES             Location/Qualifiers
     source          1..12806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     3295..3319
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3424..4125
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(3557..3575)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3825..3844
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4019..4042
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             4135..4155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4159..4179
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4183..4203
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4252..5379
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             5380..5433
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5428..6228
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5488..5507)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6098..6117
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6273..6296)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6670..6689
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6755..6764
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6776..6796
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6800..6820
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6824..6844
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(6854..6856,6857..6859,6860..7570,7571..7576,
                     7577..7696)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(6899..6920)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7160..7179)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             7697..7756
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             7757..8782
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7757..8782
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             10015..10248
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10320..10441
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10357..10376)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10411..10430
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10543..10562
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(10827..10845)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10913..11017
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11018..11086,11087..11878)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11236..11255)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12049..12637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12538..12557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgatt tcgaacctag aggacgtgac
     2281 aaacagaagc cacacgtcct agggacgtgg ggagtgcgtg aggagtacgt gaggacacgt
     2341 gggtactaga ggacgtgaca aacagaagcc acacgtccta gggacgtggg gagtgcgtga
     2401 ggagtacgtg aggacacgtg ggtactagag gacgtgacaa acagaagcca cacgtcctag
     2461 ggacgtgggg agtgcgtgag gagtacgtga ggacacgtgg gtactagagg acgtgacaaa
     2521 cagaagccac acgtcctagg gacgtgggga gtgcgtgagg agtacgtgag gacacgtggg
     2581 tactagagga cgtgacaaac agaagccaca cgtcctaggg acgtggggag tgcgtgagga
     2641 gtacgtgagg acacgtgggt actagaggac gtgacaaaca gaagccacac gtcctaggga
     2701 cgtggggagt gcgtgaggag tacgtgagga cacgtgggta ctagaggacg tgacaaacag
     2761 aagccacacg tcctagggac gtggggagtg cgtgaggagt acgtgaggac acgtgggtac
     2821 tagaggacgt gacaaacaga agccacacgt cctagggacg tggggagtgc gtgaggagta
     2881 cgtgaggaca cgtgggtact agaggacgtg acaaacagaa gccacacgtc ctagggacgt
     2941 ggggagtgcg tgaggagtac gtgaggacac gtgggtacta gaggacgtga caaacagaag
     3001 ccacacgtcc tagggacgtg gggagtgcgt gaggagtacg tgaggacacg tgggtactag
     3061 aggacgtgac aaacagaagc cacacgtcct agggacgtgg ggagtgcgtg aggagtacgt
     3121 gaggacacgt gggtactaga ggacgtgaca aacagaagcc acacgtccta gggacgtggg
     3181 gagtgcgtga ggagtacgtg aggacacgtg ggtactagag gagcatgccc atatcactag
     3241 ggtttaaacg gcgcgccagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg
     3301 tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt
     3361 tccgtaccac ttcctaccct cgtaaagtcg acaccggggc ccagatctat cgatcggccg
     3421 gatatggtga gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg
     3481 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     3541 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
     3601 gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac
     3661 atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     3721 ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg
     3781 atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag
     3841 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag
     3901 ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag
     3961 accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag
     4021 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag
     4081 ggccgccacc acctgttcct gtacggcatg gacgagctgt acaaggccgt ggatccaaaa
     4141 aagaagagaa aggtagatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag
     4201 gtacacggcg ccacgaagca agcaggagat gttgaagaaa accccgggcc tatggcttcg
     4261 taccccggcc atcaacacgc gtctgcgttc gaccaggctg cgcgttctcg cggccatagc
     4321 aaccgacgta cggcgttgcg ccctcgccgg cagcaagaag ccacggaagt ccgcccggag
     4381 cagaaaatgc ccacgctact gcgggtttat atagacggtc cccacgggat ggggaaaacc
     4441 accaccacgc aactgctggt ggccctgggt tcgcgcgacg atatcgtcta cgtacccgag
     4501 ccgatgactt actggcgggt gctgggggct tccgagacaa tcgcgaacat ctacaccaca
     4561 caacaccgcc tcgaccaggg tgagatatcg gccggggacg cggcggtggt aatgacaagc
     4621 gcccagataa caatgggcat gccttatgcc gtgaccgacg ccgttctggc tcctcatatc
     4681 gggggggagg ctgggagctc acatgccccg cccccggccc tcaccctcat cttcgaccgc
     4741 catcccatcg ccgccctcct gtgctacccg gccgcgcggt accttatggg cagcatgacc
     4801 ccccaggccg tgctggcgtt cgtggccctc atcccgccga ccttgcccgg caccaacatc
     4861 gtgcttgggg cccttccgga ggacagacac atcgaccgcc tggccaaacg ccagcgcccc
     4921 ggcgagcggc tggacctggc tatgctggct gcgattcgcc gcgtttacgg gctacttgcc
     4981 aatacggtgc ggtatctgca gtgcggcggg tcgtggcggg aggactgggg acagctttcg
     5041 gggacggccg tgccgcccca gggtgccgag ccccagagca acgcgggccc acgaccccat
     5101 atcggggaca cgttatttac cctgtttcgg gcccccgagt tgctggcccc caacggcgac
     5161 ctgtataacg tgtttgcctg ggccttggac gtcttggcca aacgcctccg ttccatgcac
     5221 gtctttatcc tggattacga ccaatcgccc gccggctgcc gggacgccct gctgcaactt
     5281 acctccggga tggtccagac ccacgtcacc acccccggct ccataccgac gatatgcgac
     5341 ctggcgcgca cgtttgcccg ggagatgggg gaggctaacg agggcagagg aagtcttcta
     5401 acatgcggtg acgtggagga gaatcccggc cctatgattg aacaagatgg attgcacgca
     5461 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5521 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5581 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5641 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5701 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5761 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5821 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5881 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5941 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt gacccatggc
     6001 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     6061 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6121 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6181 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaaa ttctaccggg
     6241 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     6301 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     6361 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     6421 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     6481 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     6541 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcaggggcgg ggcgggcgcc
     6601 cgaaggtcct ccggaggccy ggyrkkctca sggsyksrrm gggcgcccga aggtcctccg
     6661 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     6721 catctccggg cctttcgacc tgcatctcga ggtcgccacc atggtgcacg tggatccaaa
     6781 aaagaagaga aaggtagatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa
     6841 ggtacacgtg tccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     6901 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     6961 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     7021 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     7081 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     7141 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     7201 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     7261 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     7321 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     7381 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     7441 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     7501 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     7561 gctgtacaag aagcttagcc atggcttccc gccggaggtg gaggagcagg atgatggcac
     7621 gctgcccatg tcttgtgccc aggagagcgg gatggaccgt caccctgcag cctgtgcttc
     7681 tgctaggatc aatgtgcagt gtactaatta tgctctcttg aaattggctg gagatgttga
     7741 gagcaaccca ggtcccatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     7801 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     7861 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     7921 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     7981 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     8041 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     8101 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     8161 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     8221 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     8281 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     8341 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     8401 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     8461 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     8521 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     8581 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     8641 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     8701 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     8761 tcgtccgagg gcaaaggaat aggctagcct agtgccattt gttcagtggt tcgtagggct
     8821 ttcccccact gtttggcttt cagttatatg gatgatgtgg tattgggggc caagtctgta
     8881 cagcatcttg agtccctttt taccgctgtt accaattttc ttttgtcttt gggtatacat
     8941 ttaaacccta acaaaacaaa gagatggggt tactctctaa attttatggg ttatgtcatt
     9001 ggatgttatg ggtccttgcc acaagaacac atcatacaaa aaatcaaaga atgttttaga
     9061 aaacttccta ttaacaggcc tattgattgg aaagtatgtc aacgaattgt gggtcttttg
     9121 ggttttgctg ccccttttac acaatgtggt tatcctgcgt tgatgccttt gtatgcatgt
     9181 attcaatcta agcaggcttt cactttctcg ccaacttaca aggcctttct gtgtaaacaa
     9241 tacctgaacc tttaccccgt tgcccggcaa cggccaggtc tgtgccaagt gtttgctgac
     9301 gcaaccccca ctggctgggg cttggtcatg ggccatcagc gcatgcgtgg aaccttttcg
     9361 gctcctctgc cgatccatac tgcggaactc ctagccgctt gttttgctcg cagcaggtct
     9421 ggagcaaaca ttatcgggac tgataactct gttgtcctat cccgcaaata tacatcgttt
     9481 ccatggctgc taggctgtgc tgccaactgg atcctgcgcg ggacgtcctt tgtttacgtc
     9541 ccgtcggcgc tgaatcctgc ggacgaccct tctcggggtc gcttgggact ctctcgtccc
     9601 cttctccgtc tgccgttccg accgaccacg gggcgcacct ctctttacgc ggactccccg
     9661 tctgtgcctt ctcatctgcc ggaccgtgtg cacttcgctt cacctctgca cgtcgcatgg
     9721 agaccaccgt gaacgcccac caaatattgc ccaaggtctt acataagagg actcttggac
     9781 tctcagcaat gtcaacgacc gaccttgagg catacttcaa agactgtttg tttaaagact
     9841 gggaggagtt gggggaggag attaggttaa aggtctttgt actaggaggc tgtaggcata
     9901 aattggtctg cgcaccagca ccatggcgca atcactagag cggggtacct ttaagaccaa
     9961 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
    10021 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
    10081 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    10141 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    10201 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
    10261 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
    10321 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10381 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10441 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
    10501 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    10561 ggccggatcc cttgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
    10621 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
    10681 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
    10741 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
    10801 ccttgttaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
    10861 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
    10921 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
    10981 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
    11041 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
    11101 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
    11161 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
    11221 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
    11281 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    11341 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    11401 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    11461 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    11521 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
    11581 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    11641 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    11701 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    11761 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    11821 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    11881 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    11941 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    12001 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    12061 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    12121 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    12181 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    12241 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    12301 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    12361 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    12421 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    12481 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    12541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    12601 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    12661 acggttcctg gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct
    12721 ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
    12781 ggggaaggcc atccagcctc gcgtcg
//
