LOCUS       sequence-354684-       12735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LV REPORT containing minimal promoter driving monomeric nuclear 
            Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear 
            d2eGFP E2A Hygromycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell
            DL, Peet DJ, Whitelaw ML, Bersten DC
  TITLE     dFLASH; dual FLuorescent transcription factor Activity Sensor for 
            Histone integrated live-cell reporting and high-content screening
  JOURNAL   bioRxiv 2023.11.21.568191
REFERENCE   2  (bases 1 to 12735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.11.21.568191"
COMMENT     Sequence Label: pLV-REPORT(EF1a)-TTN
FEATURES             Location/Qualifiers
     source          1..12735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2325..2349
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2454..3155
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2587..2605)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2855..2874
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3049..3072
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3165..3185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3189..3209
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3213..3233
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3282..4409
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             4410..4463
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4458..5258
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4518..4537)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5128..5147
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     promoter        5419..6605
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5650..6596
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6553..6573
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6684..6693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6705..6725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6729..6749
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6753..6773
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(6783..6785,6786..6788,6789..7499,7500..7505,
                     7506..7625)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(6828..6849)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7089..7108)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             7626..7685
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             7686..8711
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7686..8711
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             9944..10177
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10249..10370
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10286..10305)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10340..10359
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10472..10491
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(10756..10774)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10842..10946
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10947..11015,11016..11807)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11165..11184)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11978..12566
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12467..12486
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgatt tcgaacctag ggtttaaacg
     2281 gcgcgccagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg tttagtgaac
     2341 cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
     2401 ttcctaccct cgtaaagtcg acaccggggc ccagatctat cgatcggccg gatatggtga
     2461 gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca
     2521 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     2581 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     2641 ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt
     2701 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     2761 gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg
     2821 tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg
     2881 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     2941 accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca
     3001 tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca
     3061 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc
     3121 acctgttcct gtacggcatg gacgagctgt acaaggccgt ggatccaaaa aagaagagaa
     3181 aggtagatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtacacggcg
     3241 ccacgaagca agcaggagat gttgaagaaa accccgggcc tatggcttcg taccccggcc
     3301 atcaacacgc gtctgcgttc gaccaggctg cgcgttctcg cggccatagc aaccgacgta
     3361 cggcgttgcg ccctcgccgg cagcaagaag ccacggaagt ccgcccggag cagaaaatgc
     3421 ccacgctact gcgggtttat atagacggtc cccacgggat ggggaaaacc accaccacgc
     3481 aactgctggt ggccctgggt tcgcgcgacg atatcgtcta cgtacccgag ccgatgactt
     3541 actggcgggt gctgggggct tccgagacaa tcgcgaacat ctacaccaca caacaccgcc
     3601 tcgaccaggg tgagatatcg gccggggacg cggcggtggt aatgacaagc gcccagataa
     3661 caatgggcat gccttatgcc gtgaccgacg ccgttctggc tcctcatatc gggggggagg
     3721 ctgggagctc acatgccccg cccccggccc tcaccctcat cttcgaccgc catcccatcg
     3781 ccgccctcct gtgctacccg gccgcgcggt accttatggg cagcatgacc ccccaggccg
     3841 tgctggcgtt cgtggccctc atcccgccga ccttgcccgg caccaacatc gtgcttgggg
     3901 cccttccgga ggacagacac atcgaccgcc tggccaaacg ccagcgcccc ggcgagcggc
     3961 tggacctggc tatgctggct gcgattcgcc gcgtttacgg gctacttgcc aatacggtgc
     4021 ggtatctgca gtgcggcggg tcgtggcggg aggactgggg acagctttcg gggacggccg
     4081 tgccgcccca gggtgccgag ccccagagca acgcgggccc acgaccccat atcggggaca
     4141 cgttatttac cctgtttcgg gcccccgagt tgctggcccc caacggcgac ctgtataacg
     4201 tgtttgcctg ggccttggac gtcttggcca aacgcctccg ttccatgcac gtctttatcc
     4261 tggattacga ccaatcgccc gccggctgcc gggacgccct gctgcaactt acctccggga
     4321 tggtccagac ccacgtcacc acccccggct ccataccgac gatatgcgac ctggcgcgca
     4381 cgtttgcccg ggagatgggg gaggctaacg agggcagagg aagtcttcta acatgcggtg
     4441 acgtggagga gaatcccggc cctatgattg aacaagatgg attgcacgca ggttctccgg
     4501 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4561 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4621 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     4681 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     4741 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4801 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     4861 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     4921 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     4981 ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt gacccatggc gatgcctgct
     5041 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5101 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5161 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5221 gcatcgcctt ctatcgcctt cttgacgagt tcttctgacg cccgccccac gacccgcagc
     5281 gcccgaccga aaggagcgac cgtcgacctc gacggatcgg gagatctcga ccgagctttg
     5341 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
     5401 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     5461 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     5521 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     5581 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     5641 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     5701 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
     5761 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     5821 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     5881 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     5941 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatcg atctgcacac
     6001 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     6061 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     6121 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     6181 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     6241 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     6301 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     6361 ggcgccgtcc aggcacctcg attagttctc gatcgagctt ttggagtacg tcgtctttag
     6421 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     6481 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     6541 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     6601 cgtgaggaat tagcttgacc aagcttggta ccgagctcgg atccactagt aacggccgcc
     6661 agtgtgctgg aattctcgag gtcgccacca tggtgcacgt ggatccaaaa aagaagagaa
     6721 aggtagatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtacacgtgt
     6781 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     6841 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     6901 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     6961 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     7021 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     7081 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     7141 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     7201 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     7261 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     7321 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     7381 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     7441 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga
     7501 agcttagcca tggcttcccg ccggaggtgg aggagcagga tgatggcacg ctgcccatgt
     7561 cttgtgccca ggagagcggg atggaccgtc accctgcagc ctgtgcttct gctaggatca
     7621 atgtgcagtg tactaattat gctctcttga aattggctgg agatgttgag agcaacccag
     7681 gtcccatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     7741 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     7801 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     7861 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     7921 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     7981 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     8041 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     8101 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     8161 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     8221 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     8281 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     8341 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     8401 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     8461 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     8521 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     8581 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     8641 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     8701 caaaggaata ggctagccta gtgccatttg ttcagtggtt cgtagggctt tcccccactg
     8761 tttggctttc agttatatgg atgatgtggt attgggggcc aagtctgtac agcatcttga
     8821 gtcccttttt accgctgtta ccaattttct tttgtctttg ggtatacatt taaaccctaa
     8881 caaaacaaag agatggggtt actctctaaa ttttatgggt tatgtcattg gatgttatgg
     8941 gtccttgcca caagaacaca tcatacaaaa aatcaaagaa tgttttagaa aacttcctat
     9001 taacaggcct attgattgga aagtatgtca acgaattgtg ggtcttttgg gttttgctgc
     9061 cccttttaca caatgtggtt atcctgcgtt gatgcctttg tatgcatgta ttcaatctaa
     9121 gcaggctttc actttctcgc caacttacaa ggcctttctg tgtaaacaat acctgaacct
     9181 ttaccccgtt gcccggcaac ggccaggtct gtgccaagtg tttgctgacg caacccccac
     9241 tggctggggc ttggtcatgg gccatcagcg catgcgtgga accttttcgg ctcctctgcc
     9301 gatccatact gcggaactcc tagccgcttg ttttgctcgc agcaggtctg gagcaaacat
     9361 tatcgggact gataactctg ttgtcctatc ccgcaaatat acatcgtttc catggctgct
     9421 aggctgtgct gccaactgga tcctgcgcgg gacgtccttt gtttacgtcc cgtcggcgct
     9481 gaatcctgcg gacgaccctt ctcggggtcg cttgggactc tctcgtcccc ttctccgtct
     9541 gccgttccga ccgaccacgg ggcgcacctc tctttacgcg gactccccgt ctgtgccttc
     9601 tcatctgccg gaccgtgtgc acttcgcttc acctctgcac gtcgcatgga gaccaccgtg
     9661 aacgcccacc aaatattgcc caaggtctta cataagagga ctcttggact ctcagcaatg
     9721 tcaacgaccg accttgaggc atacttcaaa gactgtttgt ttaaagactg ggaggagttg
     9781 ggggaggaga ttaggttaaa ggtctttgta ctaggaggct gtaggcataa attggtctgc
     9841 gcaccagcac catggcgcaa tcactagagc ggggtacctt taagaccaat gacttacaag
     9901 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     9961 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
    10021 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    10081 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    10141 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
    10201 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
    10261 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    10321 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    10381 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
    10441 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccggatccc
    10501 ttgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca caacattgcc
    10561 tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg tacctcccag
    10621 gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct gtgtagctac
    10681 cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc cttgttaatt
    10741 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
    10801 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    10861 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    10921 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    10981 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    11041 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    11101 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    11161 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
    11221 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    11281 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    11341 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    11401 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    11461 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
    11521 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    11581 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    11641 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    11701 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    11761 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    11821 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    11881 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    11941 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    12001 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    12061 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    12121 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    12181 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    12241 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    12301 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    12361 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    12421 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    12481 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    12541 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    12601 ccttttgctg gccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg
    12661 cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca
    12721 tccagcctcg cgtcg
//
