LOCUS       sequence-354688-       11836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LV REPORT containing minimal promoter driving monomeric nuclear 
            Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear 
            d2eGFP E2A Hygromycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11836)
  AUTHORS   Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell
            DL, Peet DJ, Whitelaw ML, Bersten DC
  TITLE     dFLASH; dual FLuorescent transcription factor Activity Sensor for 
            Histone integrated live-cell reporting and high-content screening
  JOURNAL   bioRxiv 2023.11.21.568191
REFERENCE   2  (bases 1 to 11836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.11.21.568191"
COMMENT     Sequence Label: pLV-REPORT(PGK)
FEATURES             Location/Qualifiers
     source          1..11836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2325..2349
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2454..3155
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2587..2605)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2855..2874
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3049..3072
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3165..3185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3189..3209
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3213..3233
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3282..4409
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             4410..4463
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4458..5258
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4518..4537)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5128..5147
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5303..5326)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5700..5719
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      5785..5794
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5806..5826
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5830..5850
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5854..5874
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5884..5886,5887..5889,5890..6600,6601..6606,
                     6607..6726)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(5929..5950)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6190..6209)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             6727..6786
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6787..7812
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6787..7812
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             9045..9278
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9350..9471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9387..9406)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9441..9460
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9573..9592
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(9857..9875)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9943..10047
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10048..10116,10117..10908)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10266..10285)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11079..11667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11568..11587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgatt tcgaacctag ggtttaaacg
     2281 gcgcgccagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg tttagtgaac
     2341 cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
     2401 ttcctaccct cgtaaagtcg acaccggggc ccagatctat cgatcggccg gatatggtga
     2461 gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca
     2521 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     2581 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     2641 ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt
     2701 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     2761 gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg
     2821 tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg
     2881 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     2941 accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca
     3001 tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca
     3061 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc
     3121 acctgttcct gtacggcatg gacgagctgt acaaggccgt ggatccaaaa aagaagagaa
     3181 aggtagatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtacacggcg
     3241 ccacgaagca agcaggagat gttgaagaaa accccgggcc tatggcttcg taccccggcc
     3301 atcaacacgc gtctgcgttc gaccaggctg cgcgttctcg cggccatagc aaccgacgta
     3361 cggcgttgcg ccctcgccgg cagcaagaag ccacggaagt ccgcccggag cagaaaatgc
     3421 ccacgctact gcgggtttat atagacggtc cccacgggat ggggaaaacc accaccacgc
     3481 aactgctggt ggccctgggt tcgcgcgacg atatcgtcta cgtacccgag ccgatgactt
     3541 actggcgggt gctgggggct tccgagacaa tcgcgaacat ctacaccaca caacaccgcc
     3601 tcgaccaggg tgagatatcg gccggggacg cggcggtggt aatgacaagc gcccagataa
     3661 caatgggcat gccttatgcc gtgaccgacg ccgttctggc tcctcatatc gggggggagg
     3721 ctgggagctc acatgccccg cccccggccc tcaccctcat cttcgaccgc catcccatcg
     3781 ccgccctcct gtgctacccg gccgcgcggt accttatggg cagcatgacc ccccaggccg
     3841 tgctggcgtt cgtggccctc atcccgccga ccttgcccgg caccaacatc gtgcttgggg
     3901 cccttccgga ggacagacac atcgaccgcc tggccaaacg ccagcgcccc ggcgagcggc
     3961 tggacctggc tatgctggct gcgattcgcc gcgtttacgg gctacttgcc aatacggtgc
     4021 ggtatctgca gtgcggcggg tcgtggcggg aggactgggg acagctttcg gggacggccg
     4081 tgccgcccca gggtgccgag ccccagagca acgcgggccc acgaccccat atcggggaca
     4141 cgttatttac cctgtttcgg gcccccgagt tgctggcccc caacggcgac ctgtataacg
     4201 tgtttgcctg ggccttggac gtcttggcca aacgcctccg ttccatgcac gtctttatcc
     4261 tggattacga ccaatcgccc gccggctgcc gggacgccct gctgcaactt acctccggga
     4321 tggtccagac ccacgtcacc acccccggct ccataccgac gatatgcgac ctggcgcgca
     4381 cgtttgcccg ggagatgggg gaggctaacg agggcagagg aagtcttcta acatgcggtg
     4441 acgtggagga gaatcccggc cctatgattg aacaagatgg attgcacgca ggttctccgg
     4501 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4561 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4621 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     4681 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     4741 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4801 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     4861 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     4921 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     4981 ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt gacccatggc gatgcctgct
     5041 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5101 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5161 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5221 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaaa ttctaccggg taggggaggc
     5281 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     5341 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     5401 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     5461 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     5521 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     5581 atagcagctt tgctccttcg ctttctgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     5641 cckgmggcyy ksyrkkctsm rsgscggggc gggcgcccga aggtcctccg gaggcccggc
     5701 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     5761 cctttcgacc tgcatctcga ggtcgccacc atggtgcacg tggatccaaa aaagaagaga
     5821 aaggtagatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa ggtacacgtg
     5881 tccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     5941 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     6001 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     6061 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     6121 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     6181 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     6241 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     6301 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     6361 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     6421 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     6481 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     6541 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     6601 aagcttagcc atggcttccc gccggaggtg gaggagcagg atgatggcac gctgcccatg
     6661 tcttgtgccc aggagagcgg gatggaccgt caccctgcag cctgtgcttc tgctaggatc
     6721 aatgtgcagt gtactaatta tgctctcttg aaattggctg gagatgttga gagcaaccca
     6781 ggtcccatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     6841 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     6901 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     6961 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     7021 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     7081 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     7141 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     7201 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     7261 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     7321 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     7381 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     7441 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     7501 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     7561 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     7621 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     7681 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     7741 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     7801 gcaaaggaat aggctagcct agtgccattt gttcagtggt tcgtagggct ttcccccact
     7861 gtttggcttt cagttatatg gatgatgtgg tattgggggc caagtctgta cagcatcttg
     7921 agtccctttt taccgctgtt accaattttc ttttgtcttt gggtatacat ttaaacccta
     7981 acaaaacaaa gagatggggt tactctctaa attttatggg ttatgtcatt ggatgttatg
     8041 ggtccttgcc acaagaacac atcatacaaa aaatcaaaga atgttttaga aaacttccta
     8101 ttaacaggcc tattgattgg aaagtatgtc aacgaattgt gggtcttttg ggttttgctg
     8161 ccccttttac acaatgtggt tatcctgcgt tgatgccttt gtatgcatgt attcaatcta
     8221 agcaggcttt cactttctcg ccaacttaca aggcctttct gtgtaaacaa tacctgaacc
     8281 tttaccccgt tgcccggcaa cggccaggtc tgtgccaagt gtttgctgac gcaaccccca
     8341 ctggctgggg cttggtcatg ggccatcagc gcatgcgtgg aaccttttcg gctcctctgc
     8401 cgatccatac tgcggaactc ctagccgctt gttttgctcg cagcaggtct ggagcaaaca
     8461 ttatcgggac tgataactct gttgtcctat cccgcaaata tacatcgttt ccatggctgc
     8521 taggctgtgc tgccaactgg atcctgcgcg ggacgtcctt tgtttacgtc ccgtcggcgc
     8581 tgaatcctgc ggacgaccct tctcggggtc gcttgggact ctctcgtccc cttctccgtc
     8641 tgccgttccg accgaccacg gggcgcacct ctctttacgc ggactccccg tctgtgcctt
     8701 ctcatctgcc ggaccgtgtg cacttcgctt cacctctgca cgtcgcatgg agaccaccgt
     8761 gaacgcccac caaatattgc ccaaggtctt acataagagg actcttggac tctcagcaat
     8821 gtcaacgacc gaccttgagg catacttcaa agactgtttg tttaaagact gggaggagtt
     8881 gggggaggag attaggttaa aggtctttgt actaggaggc tgtaggcata aattggtctg
     8941 cgcaccagca ccatggcgca atcactagag cggggtacct ttaagaccaa tgacttacaa
     9001 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     9061 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     9121 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9181 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9241 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     9301 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     9361 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9421 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9481 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9541 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccggatcc
     9601 cttgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc
     9661 ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca
     9721 ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta
     9781 ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaat
     9841 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     9901 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9961 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
    10021 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
    10081 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
    10141 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
    10201 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
    10261 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
    10321 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
    10381 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
    10441 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
    10501 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
    10561 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
    10621 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    10681 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    10741 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
    10801 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    10861 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    10921 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    10981 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    11041 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    11101 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    11161 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    11221 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    11281 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    11341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    11401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    11461 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    11521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    11581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    11641 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    11701 gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg
    11761 gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc
    11821 atccagcctc gcgtcg
//
