LOCUS       sequence-354699-       12744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LV REPORT containing minimal promoter driving monomeric nuclear 
            Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving 
            nuclear d2eGFP E2A Hygromycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12744)
  AUTHORS   Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell
            DL, Peet DJ, Whitelaw ML, Bersten DC
  TITLE     dFLASH; dual FLuorescent transcription factor Activity Sensor for 
            Histone integrated live-cell reporting and high-content screening
  JOURNAL   bioRxiv 2023.11.21.568191
REFERENCE   2  (bases 1 to 12744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.11.21.568191"
COMMENT     Sequence Label: pLV-REPORT(PGK/CMV)
FEATURES             Location/Qualifiers
     source          1..12744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2325..2349
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2454..3155
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2587..2605)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2855..2874
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3049..3072
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3165..3185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3189..3209
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3213..3233
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3282..4409
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             4410..4463
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4458..5258
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4518..4537)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5128..5147
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5303..5326)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5653..5672
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(5775..5794)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5966..6345
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6346..6549
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6500..6520
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6594..6613
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6594..6612
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6642..6666
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6693..6702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6714..6734
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6738..6758
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6762..6782
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(6792..6794,6795..6797,6798..7508,7509..7514,
                     7515..7634)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(6837..6858)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7098..7117)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             7635..7694
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             7695..8720
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7695..8720
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             9953..10186
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10258..10379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10295..10314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10349..10368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10481..10500
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(10765..10783)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10851..10955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10956..11024,11025..11816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11174..11193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11987..12575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12476..12495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat kwkmwygmyy wyrwmmctag rgkwyrwrms
     2281 rmrcrsmagc ywyasgcgtg tacggtgggc gcctataaaa gcagagctcg tttagtgaac
     2341 cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
     2401 ttcctaccct cgtaaagtcg acaccggggc ccagatctat cgatcggccg gatatggtga
     2461 gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca
     2521 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     2581 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     2641 ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt
     2701 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     2761 gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg
     2821 tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg
     2881 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     2941 accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca
     3001 tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca
     3061 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc
     3121 acctgttcct gtacggcatg gacgagctgt acaaggccgt ggatccaaaa aagaagagaa
     3181 aggtagatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtacacggcg
     3241 ccacgaagca agcaggagat gttgaagaaa accccgggcc tatggcttcg taccccggcc
     3301 atcaacacgc gtctgcgttc gaccaggctg cgcgttctcg cggccatagc aaccgacgta
     3361 cggcgttgcg ccctcgccgg cagcaagaag ccacggaagt ccgcccggag cagaaaatgc
     3421 ccacgctact gcgggtttat atagacggtc cccacgggat ggggaaaacc accaccacgc
     3481 aactgctggt ggccctgggt tcgcgcgacg atatcgtcta cgtacccgag ccgatgactt
     3541 actggcgggt gctgggggct tccgagacaa tcgcgaacat ctacaccaca caacaccgcc
     3601 tcgaccaggg tgagatatcg gccggggacg cggcggtggt aatgacaagc gcccagataa
     3661 caatgggcat gccttatgcc gtgaccgacg ccgttctggc tcctcatatc gggggggagg
     3721 ctgggagctc acatgccccg cccccggccc tcaccctcat cttcgaccgc catcccatcg
     3781 ccgccctcct gtgctacccg gccgcgcggt accttatggg cagcatgacc ccccaggccg
     3841 tgctggcgtt cgtggccctc atcccgccga ccttgcccgg caccaacatc gtgcttgggg
     3901 cccttccgga ggacagacac atcgaccgcc tggccaaacg ccagcgcccc ggcgagcggc
     3961 tggacctggc tatgctggct gcgattcgcc gcgtttacgg gctacttgcc aatacggtgc
     4021 ggtatctgca gtgcggcggg tcgtggcggg aggactgggg acagctttcg gggacggccg
     4081 tgccgcccca gggtgccgag ccccagagca acgcgggccc acgaccccat atcggggaca
     4141 cgttatttac cctgtttcgg gcccccgagt tgctggcccc caacggcgac ctgtataacg
     4201 tgtttgcctg ggccttggac gtcttggcca aacgcctccg ttccatgcac gtctttatcc
     4261 tggattacga ccaatcgccc gccggctgcc gggacgccct gctgcaactt acctccggga
     4321 tggtccagac ccacgtcacc acccccggct ccataccgac gatatgcgac ctggcgcgca
     4381 cgtttgcccg ggagatgggg gaggctaacg agggcagagg aagtcttcta acatgcggtg
     4441 acgtggagga gaatcccggc cctatgattg aacaagatgg attgcacgca ggttctccgg
     4501 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4561 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4621 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     4681 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     4741 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4801 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     4861 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     4921 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     4981 ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt gacccatggc gatgcctgct
     5041 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5101 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5161 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5221 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaaa ttctaccggg taggggaggc
     5281 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     5341 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     5401 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     5461 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     5521 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     5581 atagcagctt tgctccttcg ctttctgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     5641 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     5701 cctcatctcc gggcctttcg acctgcatct cgacggatcg ggagatctcc cgatccccta
     5761 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     5821 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     5881 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     5941 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     6001 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     6061 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     6121 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     6181 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     6241 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     6301 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     6361 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     6421 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     6481 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     6541 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
     6601 actcactata gggagaccca agctggctag ttaagctatc aacaagtttg tacaaaaaag
     6661 caggctttaa aggaaccaat tcagtcgagg tcgccaccat ggtgcacgtg gatccaaaaa
     6721 agaagagaaa ggtagatcca aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg
     6781 tacacgtgtc catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     6841 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     6901 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     6961 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     7021 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     7081 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     7141 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     7201 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     7261 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     7321 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     7381 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     7441 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     7501 tgtacaagaa gcttagccat ggcttcccgc cggaggtgga ggagcaggat gatggcacgc
     7561 tgcccatgtc ttgtgcccag gagagcggga tggaccgtca ccctgcagcc tgtgcttctg
     7621 ctaggatcaa tgtgcagtgt actaattatg ctctcttgaa attggctgga gatgttgaga
     7681 gcaacccagg tcccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     7741 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     7801 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     7861 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     7921 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     7981 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     8041 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     8101 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     8161 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     8221 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     8281 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     8341 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     8401 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     8461 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     8521 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     8581 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     8641 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     8701 gtccgagggc aaaggaatag gctagcctag tgccatttgt tcagtggttc gtagggcttt
     8761 cccccactgt ttggctttca gttatatgga tgatgtggta ttgggggcca agtctgtaca
     8821 gcatcttgag tcccttttta ccgctgttac caattttctt ttgtctttgg gtatacattt
     8881 aaaccctaac aaaacaaaga gatggggtta ctctctaaat tttatgggtt atgtcattgg
     8941 atgttatggg tccttgccac aagaacacat catacaaaaa atcaaagaat gttttagaaa
     9001 acttcctatt aacaggccta ttgattggaa agtatgtcaa cgaattgtgg gtcttttggg
     9061 ttttgctgcc ccttttacac aatgtggtta tcctgcgttg atgcctttgt atgcatgtat
     9121 tcaatctaag caggctttca ctttctcgcc aacttacaag gcctttctgt gtaaacaata
     9181 cctgaacctt taccccgttg cccggcaacg gccaggtctg tgccaagtgt ttgctgacgc
     9241 aacccccact ggctggggct tggtcatggg ccatcagcgc atgcgtggaa ccttttcggc
     9301 tcctctgccg atccatactg cggaactcct agccgcttgt tttgctcgca gcaggtctgg
     9361 agcaaacatt atcgggactg ataactctgt tgtcctatcc cgcaaatata catcgtttcc
     9421 atggctgcta ggctgtgctg ccaactggat cctgcgcggg acgtcctttg tttacgtccc
     9481 gtcggcgctg aatcctgcgg acgacccttc tcggggtcgc ttgggactct ctcgtcccct
     9541 tctccgtctg ccgttccgac cgaccacggg gcgcacctct ctttacgcgg actccccgtc
     9601 tgtgccttct catctgccgg accgtgtgca cttcgcttca cctctgcacg tcgcatggag
     9661 accaccgtga acgcccacca aatattgccc aaggtcttac ataagaggac tcttggactc
     9721 tcagcaatgt caacgaccga ccttgaggca tacttcaaag actgtttgtt taaagactgg
     9781 gaggagttgg gggaggagat taggttaaag gtctttgtac taggaggctg taggcataaa
     9841 ttggtctgcg caccagcacc atggcgcaat cactagagcg gggtaccttt aagaccaatg
     9901 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     9961 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
    10021 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    10081 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    10141 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
    10201 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
    10261 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10321 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10381 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
    10441 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    10501 ccggatccct tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
    10561 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
    10621 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
    10681 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
    10741 ttgttaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
    10801 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
    10861 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    10921 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    10981 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    11041 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    11101 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    11161 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
    11221 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    11281 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    11341 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    11401 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    11461 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
    11521 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    11581 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    11641 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    11701 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    11761 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    11821 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    11881 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    11941 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    12001 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    12061 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    12121 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    12181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    12241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    12301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    12361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    12421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    12481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    12541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    12601 ggttcctggc cttttgctgg ccttgaagct gtccctgatg gtcgtcatct acctgcctgg
    12661 acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg
    12721 ggaaggccat ccagcctcgc gtcg
//
