LOCUS       sequence-354709-        2287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter SPLc25 and RBSc249 in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Taylor GM, Hitchcock A, Heap JT
  TITLE     Combinatorial assembly platform enabling engineering of genetically 
            stable metabolic pathways in cyanobacteria.
  JOURNAL   Nucleic Acids Res. 2021 Dec 2;49(21):e123. doi: 10.1093/nar/gkab791.
  PUBMED    34554258
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2021-12-2"; volume: "49(21):e123. doi"; pages: "
            10.1093/nar/gkab791"
COMMENT     Sequence Label: pStA0::SPLc25-RBsc249
FEATURES             Location/Qualifiers
     source          1..2287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(58..76)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        144..248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(249..317,318..1109)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(467..486)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1280..1868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1769..1788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2026..2120
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(join(2246..2287,1..45))
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc cgaaagggcc
       61 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      181 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      241 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      301 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      361 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      421 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      481 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      541 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      601 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      661 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      721 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      781 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      841 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      901 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      961 gtgggtcccg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     1021 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     1081 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1141 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1201 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1261 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1321 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1381 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1441 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1501 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1561 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1621 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1681 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1741 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1801 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1861 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1921 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1981 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg ccttggactc ctgttgatag
     2041 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
     2101 cgttttttat tggtgagaat ccagtgtggc tcttcacagc cacgaaaaat tgacaagcac
     2161 tctgcgctct gatataatta aggtggcgcg agtcaacgtt cctgaggagt ggcatgtgaa
     2221 gagcgtaaga cctctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac
     2281 aacagat
//
