LOCUS       sequence-354712-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of membrane protein with N-terminal FLAG tag and 
            C-terminal split GFP1-10 fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Bruguera ES, Mahoney JP, Weis WI
  TITLE     Reconstitution of purified membrane protein dimers in lipid 
            nanodiscs with defined stoichiometry and orientation using a split 
            GFP tether.
  JOURNAL   J Biol Chem. 2022 Jan 21:101628. doi: 10.1016/j.jbc.2022.101628.
  PUBMED    35074428
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2022 Jan 21:101628. doi: 10.1016/j.jbc.2022.101628."
COMMENT     Sequence Label: FLAG-3C-sGFP1-10_pcDNA3.1(-)
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             181..204
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(244..272)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(891..908)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct ggctagcgtt
       61 taaacgggcc ctctagactc gagcggccgc gccaccatga agacgatcat cgccctgagc
      121 tacatcttct gcctggtgtt cgccgactac aaggacgatg atgacgccac cggtggatcc
      181 ctcgaagtcc ttttccaggg ccctggtggc actagtagca aaggagaaga acttttcact
      241 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      301 agaggagagg gtgaaggtga tgctacaatc ggaaaactca cccttaaatt tatttgcact
      361 actggaaaac tacctgttcc atggccaaca cttgtcacta ctctgaccta tggtgttcaa
      421 tgcttttccc gttatccgga tcacatgaaa aggcatgact ttttcaagag tgccatgccc
      481 gaaggttatg tacaggaacg cactatatct ttcaaagatg acgggaaata caagacgcgt
      541 gctgtagtca agtttgaagg tgataccctt gttaatcgta tcgagttaaa gggtactgat
      601 tttaaagaag atggaaacat tctcggacac aaactcgagt acaactttaa ctcacacaat
      661 gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcac agttcgccac
      721 aacgttgaag atggttccgt tcaactagca gaccattatc aacaaaatac tccaattggc
      781 gatggccctg tccttttacc agacaaccat tacctgtcga cacaaactgt cctttcgaaa
      841 gatcccaacg aaaagtgagg taccaagctt aagtttaaac cgctgatcag cctcgactgt
      901 gccttcta
//
