LOCUS       sequence-354738-        9605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses untagged full length NAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9605)
  AUTHORS   Paul S, Sarraf SA, Nam KH, Zavar L, DeFoor N, Biswas SR, Fritsch LE,
            Yaron TM, Johnson JL, Huntsman EM, Cantley LC, Ordureau A, Pickrell 
            AM
  TITLE     NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate 
            mitosis and cytokinesis.
  JOURNAL   J Cell Biol. 2024 Feb 5;223(2):e202303082. doi: 
            10.1083/jcb.202303082. Epub 2023 Dec 7.
  PUBMED    38059900
REFERENCE   2  (bases 1 to 9605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2024-02-5"; volume: "223(2):e202303082. doi"; pages: 
            " 10.1083/jcb.202303082. Epub 2023 Dec 7"
COMMENT     Sequence Label: pHAGE FL NAP1 STOP C' EGFP IRES Blast
FEATURES             Location/Qualifiers
     source          1..9605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    108..225
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        277..580
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        581..784
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     735..755
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     781..805
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    911..935
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2112..2136)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2147..2149,2150..2152,2153..2866)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2147..2149,2150..2152,2153..2863)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2192..2213)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2453..2472)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2800..2821
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        2935..3434
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2970..2993)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3367..3386
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3458..3856
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3898..4019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3935..3954)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    4045..4633
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4098..4118)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4516..4527)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4708..4941
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5060..5078)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5146..5250
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5251..5319,5320..6111)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5469..5488)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6282..6870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6771..6790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7024..7041
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7281..7302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7317..7322,7323..7340,7341..7347)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7355..7371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7360..7382
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7379..7395
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7379..7395
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             7656..8289
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8336..8461
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8958..9191
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9376..9420
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
       61 atctcgacgg tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa
      121 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      181 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
      241 gacagcagag atccagtttg gactagtgga gttccgcgtt acataactta cggtaaatgg
      301 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      361 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      421 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      481 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      541 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      601 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      661 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      721 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      781 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
      841 tagaagacac cggcggccga tctaatacga ctcactatag ggagacccaa gctggctagt
      901 taagctatca acaagtttgt acaaaaaagt tggcatggat gcactggtag aagatgatat
      961 ctgtattctg aatcatgaaa aagcccataa gagagataca gtgactccag tttcaatata
     1021 ttcaggagat gaatctgttg cttcccattt tgctcttgtc actgcatatg aagacatcaa
     1081 aaaacgactt aaggattcag agaaagagaa ctctttgtta aagaagagaa taagattttt
     1141 ggaagaaaag ctaatagctc gatttgaaga agaaacaagt tccgtggaac gagaacaagt
     1201 aaataaggcc tatcatgcat atcgagaggt ttgcattgat agagataatt tgaagagcaa
     1261 actggacaaa atgaataaag acaactctga atctttgaaa gtattgaatg agcagctaca
     1321 atctaaagaa gtagaactcc tccagctgag gacagaggtg gaaactcagc aggtgatgag
     1381 gaatttaaat ccaccttcat caaactggga ggtggaaaag ttgagctgtg acctgaagat
     1441 ccatggtttg gaacaagagc tggaactgat gaggaaagaa tgtagcgatc tcaaaataga
     1501 actacagaaa gccaaacaaa cggatccata tcaggaagac aatctgaaga gcagagatct
     1561 ccaaaaacta agcatttcaa gtgataatat gcagcatgca tactgggaac tgaagagaga
     1621 aatgtctaat ttacatctgg tgactcaagt acaagctgaa ctactaagaa aactgaaaac
     1681 ctcaactgca atcaagaaag cctgtgcccc tgtaggatgc agtgaagacc ttggaagaga
     1741 cagcacaaaa ctgcacttga tgaattttac tgcaacatac acaagacatc cccctctctt
     1801 accaaatggc aaagctcttt gtcataccac atcttcccct ttaccaggag atgtaaaggt
     1861 tttatcagag aaagcaatcc tccaatcatg gacagacaat gagagatcca ttcctaatga
     1921 tggtacatgc tttcaggaac acagttctta tggcagaaat tctctggaag acaattcctg
     1981 ggtatttcca agtcctccta aatcaagtga gacagcattt ggggaaacta aaactaaaac
     2041 tttgccttta cccaaccttc caccactgca ttacttggat caacataatc agaactgcct
     2101 ttataagaat taaccaactt tcttgtacaa agtggttgat ctagagatgg tgagcaaggg
     2161 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2221 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2281 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2341 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     2401 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2461 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2521 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2581 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2641 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     2701 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     2761 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2821 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaacgcg ttacgttgga
     2881 aacgggggag gctaactgaa acacggaagg agctcgaggt taacgaattc taccgggtag
     2941 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     3001 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     3061 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     3121 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     3181 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     3241 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     3301 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     3361 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     3421 ctccgggcct ttcgacctgc agcccattaa ttaaaccatg gccaagcctt tgtctcaaga
     3481 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     3541 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     3601 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     3661 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     3721 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     3781 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     3841 tgtgtgggag ggctaagcac ttcgtggccg aggagcagga ctgacactcg acctcgaaac
     3901 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3961 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4021 catgtctatg catcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg
     4081 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4141 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4201 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4261 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4321 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4381 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4441 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4501 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4561 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4621 gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta
     4681 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     4741 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4801 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4861 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4921 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     4981 ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat
     5041 cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5101 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5161 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5221 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5281 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5341 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5401 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5461 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     5521 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5581 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5641 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5701 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5761 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5821 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5881 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5941 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6001 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6061 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6121 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6181 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6241 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6301 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6361 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6421 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6481 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6541 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6601 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6661 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6721 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6781 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6841 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6901 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6961 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7021 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7081 gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg
     7141 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7201 gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg
     7261 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7321 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7381 ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc actgcattct
     7441 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     7501 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     7561 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     7621 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
     7681 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     7741 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     7801 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     7861 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     7921 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     7981 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     8041 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     8101 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8161 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8221 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8281 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     8341 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     8401 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     8461 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     8521 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     8581 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     8641 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     8701 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     8761 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
     8821 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     8881 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     8941 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     9001 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     9061 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     9121 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     9181 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     9241 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     9301 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     9361 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     9421 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     9481 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     9541 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     9601 cccga
//
