LOCUS       sequence-354739-        9516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of full length NAP1 cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Paul S, Sarraf SA, Nam KH, Zavar L, DeFoor N, Biswas SR, Fritsch LE,
            Yaron TM, Johnson JL, Huntsman EM, Cantley LC, Ordureau A, Pickrell 
            AM
  TITLE     NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate 
            mitosis and cytokinesis.
  JOURNAL   J Cell Biol. 2024 Feb 5;223(2):e202303082. doi: 
            10.1083/jcb.202303082. Epub 2023 Dec 7.
  PUBMED    38059900
REFERENCE   2  (bases 1 to 9516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2024-02-5"; volume: "223(2):e202303082. doi"; pages: 
            " 10.1083/jcb.202303082. Epub 2023 Dec 7"
COMMENT     Sequence Label: pHAGE N' EGFP NAP1 FL IRES Blast
FEATURES             Location/Qualifiers
     source          1..9516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             91..724
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    771..896
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1393..1626
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1811..1855
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2148..2265
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2317..2620
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2621..2824
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2775..2795
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2821..2845
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2904..2913
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2910..2912,2913..2915,2916..3626)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2910..2912,2913..2915,2916..3626)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2955..2976)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3216..3235)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3563..3584
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3642..3666
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4843..4867)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4886..5385
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4921..4944)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5318..5337
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5409..5807
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5849..5970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5886..5905)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    5996..6584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6049..6069)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6467..6478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6659..6892
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7011..7029)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7097..7201
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7202..7270,7271..8062)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7420..7439)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8233..8821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8722..8741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8975..8992
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9232..9253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9268..9273,9274..9291,9292..9298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9306..9322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9311..9333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9330..9346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9330..9346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
       61 ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac
      121 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
      181 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
      241 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
      301 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
      361 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
      421 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
      481 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
      541 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      601 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      661 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      721 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      781 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      841 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      901 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
      961 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1021 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1081 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1141 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1201 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
     1261 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1321 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1381 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1441 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1501 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1561 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1621 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1681 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1741 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1801 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1861 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1921 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1981 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     2041 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2101 atctcgacgg tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa
     2161 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2221 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     2281 gacagcagag atccagtttg gactagtgga gttccgcgtt acataactta cggtaaatgg
     2341 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2401 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2461 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2521 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2581 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     2641 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     2701 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     2761 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     2821 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     2881 tagaagacac cggcggccga tctgccacca tggtgagcaa gggcgaggag ctgttcaccg
     2941 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     3001 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     3061 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     3121 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     3181 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     3241 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3301 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3361 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     3421 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3481 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     3541 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3601 ctctcggcat ggacgagctg tacaaggctc gaggttatca aacaagtttg tacaaaaaag
     3661 ttggcatgga tgcactggta gaagatgata tctgtattct gaatcatgaa aaagcccata
     3721 agagagatac agtgactcca gtttcaatat attcaggaga tgaatctgtt gcttcccatt
     3781 ttgctcttgt cactgcatat gaagacatca aaaaacgact taaggattca gagaaagaga
     3841 actctttgtt aaagaagaga ataagatttt tggaagaaaa gctaatagct cgatttgaag
     3901 aagaaacaag ttccgtggga cgagaacaag taaataaggc ctatcatgca tatcgagagg
     3961 tttgcattga tagagataat ttgaagagca aactggacaa aatgaataaa gacaactctg
     4021 aatctttgaa agtattgaat gagcagctac aatctaaaga agtagaactc ctccagctga
     4081 ggacagaggt ggaaactcag caggtgatga ggaatttaaa tccaccttca tcaaactggg
     4141 aggtggaaaa gttgagctgt gacctgaaga tccatggttt ggaacaagag ctggaactga
     4201 tgaggaaaga atgtagcgat ctcaaaatag aactacagaa agccaaacaa acggatccat
     4261 atcaggaaga caatctgaag agcagagatc tccaaaaact aagcatttca agtgataata
     4321 tgcagcatgc atactgggaa ctgaagagag aaatgtctaa tttacatctg gtgactcaag
     4381 tacaagctga actactaaga aaactgaaaa cctcaactgc aatcaagaaa gcctgtgccc
     4441 ctgtaggatg cagtgaagac cttggaagag acagcacaaa actgcacttg atgaatttta
     4501 ctgcaacata cacaagacat ccccctctct taccaaatgg caaagctctt tgtcatacca
     4561 catcttcccc tttaccagga gatgtaaagg ttttatcaga gaaagcaatc ctccaatcat
     4621 ggacagacaa tgagagatcc attcctaatg atggtacatg ctttcaggaa cacagttctt
     4681 atggcagaaa ttctctggaa gacaattcct gggtatttcc aagtcctcct aaatcaagtg
     4741 agacagcatt tggggaaact aaaactaaaa ctttgccttt acccaacctt ccaccactgc
     4801 attacttgga tcaacataat cagaactgcc tttataagaa ttaaccaact ttcttgtaca
     4861 aagtggttcg ataacgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     4921 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     4981 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     5041 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     5101 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     5161 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     5221 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     5281 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     5341 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccatta
     5401 attaaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     5461 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     5521 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     5581 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     5641 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     5701 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     5761 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca cttcgtggcc
     5821 gaggagcagg actgacactc gacctcgaaa cttgtttatt gcagcttata atggttacaa
     5881 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5941 tggtttgtcc aaactcatca atgtatctta tcatgtctat gcatcgatag atcctaatca
     6001 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6061 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6121 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6181 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6241 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6301 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6361 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     6421 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6481 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6541 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga
     6601 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6661 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6721 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6781 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6841 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6901 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6961 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     7021 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     7081 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7141 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7201 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7261 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7321 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7381 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7441 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7501 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7561 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7621 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7681 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7741 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7801 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7861 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7921 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7981 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8041 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8101 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8161 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8221 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8281 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8341 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8401 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8461 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8521 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8581 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8641 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8701 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8761 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8821 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8881 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8941 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9001 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa
     9061 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9121 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
     9181 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     9241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     9301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
     9361 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9421 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     9481 caccccatac cctattacca ctgccaatta cctgtg
//
