LOCUS       sequence-35474-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Fleishman SJ, Whitehead TA, Strauch EM, Corn JE, Qin S, Zhou HX, 
            Mitchell JC, Demerdash ON, Takeda-Shitaka M, Terashi G, Moal IH, Li 
            X, Bates PA, Zacharias M, Park H, Ko JS, Lee H, Seok C, Bourquard T,
            Bernauer J, Poupon A, Aze J, Soner S, Ovali SK, Ozbek P, Tal NB, 
            Haliloglu T, Hwang H, Vreven T, Pierce BG, Weng Z, Perez-Cano L, 
            Pons C, Fernandez-Recio J, Jiang F, Yang F, Gong X, Cao L, Xu X, Liu
            B, Wang P, Li C, Wang C, Robert CH, Guharoy M, Liu S, Huang Y, Li L,
            Guo D, Chen Y, Xiao Y, London N, Itzhaki Z, Schueler-Furman O, Inbar
            Y, Patapov V, Cohen M, Schreiber G, Tsuchiya Y, Kanamori E, Standley
            DM, Nakamura H, Kinoshita K, Driggers CM, Hall RG, Morgan JL, Hsu 
            VL, Zhan J, Yang Y, Zhou Y, Kastritis PL, Bonvin AM, Zhang W, 
            Camacho CJ, Kilambi KP, Sircar A, Gray JJ, Ohue M, Uchikoga N, 
            Matsuzaki Y, Ishida T, Akiyama Y, Khashan R, Bush S, Fouches D, 
            Tropsha A, Esquivel-Rodriguez J, Kihara D, Stranges PB, Jacak R, 
            Kuhlman B, Huang SY, Zou X, Wodak SJ, Janin J, Baker D
  TITLE     Community-Wide Assessment of Protein-Interface Modeling Suggests 
            Improvements to Design Methodology.
  JOURNAL   J Mol Biol. 2011 Sep 29.
  PUBMED    22001016
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2011 Sep 29."
COMMENT     Sequence Label: pETCON-IgG3/design_30
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             307..318
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             319..345
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             421..441
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tccctacttc atacattttc aattaagatg cagttacttc gctgtttttc aatattttct
       61 gttattgctt cagttttagc acaggaactg acaactatat gcgagcaaat cccctcacca
      121 actttagaat cgacgccgta ctctttgtca acgactacta ttttggccaa cgggaaggca
      181 atgcaaggag tttttgaata ttacaaatca gtaacgtttg tcagtaattg cggttctcac
      241 ccctcaacaa ctagcaaagg cagccccata aacacacagt atgtttttaa ggacaatagc
      301 tcgacgattg aaggtagata cccatacgac gttccagact acgctctgca ggctagtggt
      361 ggaggaggct ctggtggagg cggtagcgga ggcggagggt cggctagcca tatgggtaga
      421 gaaaatttgt acttccaagg ttctgaacat ttgttggatt tgcaatggga attgaagttg
      481 atagaattag aaaatgtcca agaaatagat gcccacatca aggactcaaa ctttttcgaa
      541 tgggttggtt ttattaaagg tccagaaggt actccttatg aaggtggtca tttcacctta
      601 gcaatcacta ttccaaatgc ctaccctttt aacccaccta aaattaagtt cgtaaccaag
      661 atttggcacc caaatatatc ttcacaaaca ggtgctatat gtttggatgt cttgaaaaat
      721 caatgggcac cttggtccag tatctcttgg gctttgttat caattcaagc attgttatcc
      781 gatccacaac ctgatgaccc acaagacgct gaagttgcaa aaatgtacaa ggaaaatcat
      841 gccttgtttg ttaaaact
//
