LOCUS       sequence-354743-       11560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged TAPBPR with MHC TM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   McShan AC, Devlin CA, Papadaki GF, Sun Y, Green AI, Morozov GI, 
            Burslem GM, Procko E, Sgourakis NG
  TITLE     TAPBPR employs a ligand-independent docking mechanism to chaperone 
            MR1 molecules.
  JOURNAL   Nat Chem Biol. 2022 Jun 20. pii: 10.1038/s41589-022-01049-9. doi: 
            10.1038/s41589-022-01049-9.
  PUBMED    35725941
REFERENCE   2  (bases 1 to 11560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 Jun 20. pii: 10.1038/s41589-022-01049-9. doi: 
            10.1038/s41589-022-01049-9."
COMMENT     Sequence Label: pCEP4-TAPBPR-TM
FEATURES             Location/Qualifiers
     source          1..11560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    126..260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(150..169)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     204..223
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(403..425)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      985..2774
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(3076..5001)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGXGGXGXGGGXGXGGGXGGGGGGGGGGGGGGGGGGGGGGGGGGG
                     GGGGGXGXGGGXGXGGGAGGAGXGGGXGGXGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGXGGXGGXGXGGXGGXGGGGGGGGGGXG
                     GGXGGXGXGGXGGXGXGGXGGXGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(5436..5454)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5522..5626
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5627..5695,5696..6487)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5845..5864)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6658..7246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7147..7166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7677..7822
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             7865..8902
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(8900..8919)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8944..8992
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        9646..10025
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10026..10229
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10180..10200
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10226..10250
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      10278..10287
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10362..10385
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgctatcccc atcatgggta tcgttgctgg cctggttgtc cttgcagctg tagtcactgg
       61 agctgcggtc gctgctgtgc tgtggagaaa gaagagctca gattgactcg aggccggcaa
      121 ggccggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
      181 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
      241 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
      301 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
      361 atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
      421 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
      481 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc tagaggatcg
      541 atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca
      601 gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt
      661 gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat
      721 ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt
      781 ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc
      841 tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat
      901 cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt
      961 tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata
     1021 ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg
     1081 aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca
     1141 cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg
     1201 gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc
     1261 attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga
     1321 ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca
     1381 gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg caataaatac
     1441 tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca
     1501 agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc
     1561 tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga
     1621 accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg
     1681 actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc
     1741 cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca
     1801 gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga
     1861 ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc
     1921 cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct
     1981 ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata
     2041 ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca
     2101 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     2161 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     2221 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     2281 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata
     2341 gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata
     2401 tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     2461 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     2521 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
     2581 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta
     2641 tccgggtagc atatgctatc ctcatgcata tacagtcagc atatgatacc cagtagtaga
     2701 gtgggagtgc tatcctttgc atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc
     2761 ttgtcctttt cctgctggtt gctcccattc ttaggtgaat ttaaggaggc caggctaaag
     2821 ccgtcgcatg tctgattgct caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg
     2881 tgttgagcgc ggagctgagt gacgtgacaa catgggtatg cccaattgcc ccatgttggg
     2941 aggacgaaaa tggtgacaag acagatggcc agaaatacac caacagcacg catgatgtct
     3001 actggggatt tattctttag tgcgggggaa tacacggctt ttaatacgat tgagggcgtc
     3061 tcctaacaag ttacatcact cctgcccttc ctcaccctca tctccatcac ctccttcatc
     3121 tccgtcatct ccgtcatcac cctccgcggc agccccttcc accataggtg gaaaccaggg
     3181 aggcaaatct actccatcgt caaagctgca cacagtcacc ctgatattgc aggtaggagc
     3241 gggctttgtc ataacaaggt ccttaatcgc atccttcaaa acctcagcaa atatatgagt
     3301 ttgtaaaaag accatgaaat aacagacaat ggactccctt agcgggccag gttgtgggcc
     3361 gggtccaggg gccattccaa aggggagacg actcaatggt gtaagacgac attgtggaat
     3421 agcaagggca gttcctcgcc ttaggttgta aagggaggtc ttactacctc catatacgaa
     3481 cacaccggcg acccaagttc cttcgtcggt agtcctttct acgtgactcc tagccaggag
     3541 agctcttaaa ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt gaccacgatg
     3601 ctttccaaac caccctcctt ttttgcgcct gcctccatca ccctgacccc ggggtccagt
     3661 gcttgggcct tctcctgggt catctgcggg gccctgctct atcgctcccg ggggcacgtc
     3721 aggctcacca tctgggccac cttcttggtg gtattcaaaa taatcggctt cccctacagg
     3781 gtggaaaaat ggccttctac ctggaggggg cctgcgcggt ggagacccgg atgatgatga
     3841 ctgactactg ggactcctgg gcctcttttc tccacgtcca cgacctctcc ccctggctct
     3901 ttcacgactt ccccccctgg ctctttcacg tcctctaccc cggcggcctc cactacctcc
     3961 tcgaccccgg cctccactac ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc
     4021 tccacctcct gctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc
     4081 ccctcctgct cctscccctc ctscccctcc tsctcctscc cctcctsccc ctcctsctcc
     4141 tscccctcct scccctcctc ctsctcctcc ccctcctccc cctcctcctc ctcctscccc
     4201 tcctscccct cctsctccts cccctcctsc ccctcctsct cctgcccctc ctgcccctcc
     4261 tgctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc
     4321 tgcccctcct gcccctcctc ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc
     4381 tgcccctcct gctcctgccc ctcctcctgc tcctgcccct cctscccctc ctscccctcc
     4441 tcctsctcct gcccctcctg cccctcctcc tsctcctscc cctcctccts ctcctscccc
     4501 tcctccccct cctccccctc ctcctcctcc tccccctcct ccycctcctc ctcctcctcc
     4561 ycctcctcct cctcctcccc ctcctccycc tcctscccct cctcctsctc ctscccctcc
     4621 tcctsctcct scccctccts cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     4681 tcctcctgct cctgcccctc ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc
     4741 accgtgggtc cctttgcagc caatgcaact tggacgtttt tggggtctcc ggacaccatc
     4801 tctatgtctt ggccctgatc ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc
     4861 tcgtcctctt ccccgtcctc gtccatggtt atcaccccct cttctttgag gtccactgcc
     4921 gccggagcct tctggtccag atgtgtctcc cttctctcct aggccatttc caggtcctgt
     4981 acctggcccc tcgtcagaca tgattcacac taaaagagat caatagacat ctttattaga
     5041 cgacgctcag tgaatacagg gagtgcagac tcctgccccc tccaacagcc cccccaccct
     5101 catccccttc atggtcgctg tcagacagat ccaggtctga aaattcccca tcctccgaac
     5161 catcctcgtc ctcatcacca attactcgca gcccggaaaa ctcccgctga acatcctcaa
     5221 gatttgcgtc ctgagcctca agccaggcct caaattcctc gtcccccttt ttgctggacg
     5281 gtagggatgg ggattctcgg gacccctcct cttcctcttc aaggtcacca gacagagatg
     5341 ctactggggc aacggaagaa aagctgggtg cggcctgtga ggatcagctt atcgatgata
     5401 agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     5461 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5521 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5581 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5641 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5701 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5761 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5821 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     5881 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5941 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6001 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6061 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6121 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
     6181 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6241 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6301 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6361 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6421 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6481 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6541 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6601 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6661 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6721 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6781 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6841 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6901 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6961 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7021 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7081 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7141 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7201 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7261 ggccttttta cggttcctgg ccttttgctg gccttgaagc tgtccctgat ggtcgtcatc
     7321 tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag
     7381 aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag
     7441 cgcgtcggcc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg
     7501 ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt
     7561 cttggagtgg tgaatccgtt agcgaggtgc cgccctgctt catccccgtg gcccgttgct
     7621 cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga
     7681 cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc
     7741 ggcgcggtcc gaggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc
     7801 gaccctgcag cgacccgctt aacagcgtca acagcgtgcc gcagatcccg gggggcaatg
     7861 agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     7921 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     7981 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     8041 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     8101 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     8161 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     8221 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     8281 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     8341 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     8401 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     8461 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     8521 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     8581 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     8641 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     8701 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     8761 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     8821 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccggatcg
     8881 ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     8941 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
     9001 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     9061 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     9121 ctcgcagcca acgtcggggc ggcaggccct gccatagcca ctggccccgt gggttaggga
     9181 cggggtcccc catggggaat ggtttatggt tcgtgggggt tattattttg ggcgttgcgt
     9241 ggggtcaggt ccacgactgg actgagcaga cagacccatg gtttttggat ggcctgggca
     9301 tggaccgcat gtactggcgc gacacgaaca ccgggcgtct gtggctgcca aacacccccg
     9361 acccccaaaa accaccgcgc ggatttctgg cgtgccaagc tagtcgacca attctcatgt
     9421 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
     9481 ggtaggtatg gaagatctat acattgaatc aatattggca attagccata ttagtcattg
     9541 gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata
     9601 atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttgacat tgattattga
     9661 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     9721 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     9781 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     9841 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     9901 caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     9961 acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
    10021 ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg
    10081 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
    10141 gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg
    10201 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc tctagaagct
    10261 gggtaccagc tgctagcgcc accatggccg tcatggcgcc ccgaaccctc gtcctgctac
    10321 tctcgggggc cctggccctg acccagacct gggcgggctc cgactacaag gacgatgatg
    10381 acaagggagg atccaagccc cacccagcag aggggcagtg gcgggcagtg gacgtggtcc
    10441 tagactgttt cctggtgaag gacggtgcgc accgtggagc tctcgccagc agtgaggaca
    10501 gggcaagggc ctcccttgtg ctgaagcagg tgccagtgct ggacgatggc tccctggagg
    10561 acttcaccga tttccaaggg ggcacactgg cccaagatga cccacctatt atctttgagg
    10621 cctcagtgga cctggtccag attccccagg ccgaggcctt gctccatgct gactgcagtg
    10681 ggaaggaggt gacctgtgag atctcccgct actttctcca gatgacagag accactgtta
    10741 agacagcagc ttggttcatg gccaacgtgc aggtctctgg agggggacct agcatctcct
    10801 tggtgatgaa gactcccagg gtcgccaaga atgaggtgct ctggcaccca acgctgaact
    10861 tgccactgag cccccagggg actgtgcgaa ctgcagtgga gttccaggtg atgacacaga
    10921 cccaatccct gagcttcctg ctggggtcct cagcctcctt ggactgtggc ttctccatgg
    10981 caccgggctt ggacctcatc agtgtggagt ggcgactgca gcacaagggc aggggtcagt
    11041 tggtgtacag ctggaccgca gggcaggggc aggctgtgcg gaagggcgct accctggagc
    11101 ctgcacaact gggcatggcc agggatgcct ccctcaccct gcccggcctc actatacagg
    11161 acgaggggac ctacatttgc cagatcacca cctctctgta ccgagctcag cagatcatcc
    11221 agctcaacat ccaagcttcc cctaaagtac gactgagctt ggcaaacgaa gctctgctgc
    11281 ccaccctcat ctgcgacatt gctggctatt accctctgga tgtggtggtg acgtggaccc
    11341 gagaggagct gggtggatcc ccagcccaag tctctggtgc ctccttctcc agcctcaggc
    11401 aaagcgtggc aggcacctac agcatctcct cctctctcac cgcagaacct ggctctgcag
    11461 gtgccactta cacctgccag gtcacacaca tctctctgga ggagcccctt ggggccagca
    11521 cccaggttgt cccaccagag cggagatctg gagccggaag
//
