LOCUS       sequence-35476-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  TITLE     pET23-CBD
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET23-CBD
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             410..432
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(463..482)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(464..482)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag agatggcggt gggggcggcg
       61 gaagtggctc ctggcgcctc atctcctgcc gcacagcctc cagcccaccc tggtcctcaa
      121 tgaagtcgta gataagttta gaggtctcgg cgtcggtgag ctgggcctcg ctgattcctg
      181 ccctggagaa cagactccgc agatctgggt cgaggttgtt cacgtcaaat ccattctggg
      241 ggtcccaccc cacgtggctg acatgcttga atccactggg tgcaccaata tcagctttgc
      301 tgatcttctt cttccctgag cgtttcttat cagctgggct aggtccaggt gctgggagcc
      361 cacggtatcg tgaactcgtg atgtcagggt tctggatgtc catggtatat ctccttctta
      421 aagttaaaca aaattatttc tagagggaaa ccgttgtggt ctccctatag tgagtcgtat
      481 taatttcgcg ggatcgagat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc
      541 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat
      601 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga
      661 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca
      721 gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga
      781 acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc
      841 cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca
      901 tcatcagtaa cccgtatcg
//
