LOCUS       sequence-354770-       11560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, 
            R207E, Q209S, Q272S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   McShan AC, Devlin CA, Papadaki GF, Sun Y, Green AI, Morozov GI, 
            Burslem GM, Procko E, Sgourakis NG
  TITLE     TAPBPR employs a ligand-independent docking mechanism to chaperone 
            MR1 molecules.
  JOURNAL   Nat Chem Biol. 2022 Jun 20. pii: 10.1038/s41589-022-01049-9. doi: 
            10.1038/s41589-022-01049-9.
  PUBMED    35725941
REFERENCE   2  (bases 1 to 11560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 Jun 20. pii: 10.1038/s41589-022-01049-9. doi: 
            10.1038/s41589-022-01049-9."
COMMENT     Sequence Label: pCEP4 TAPBPR-TM-TN6
FEATURES             Location/Qualifiers
     source          1..11560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(147..165)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        233..337
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(338..406,407..1198)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(556..575)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1369..1957
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1858..1877
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2388..2533
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2576..3613
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3611..3630)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3655..3703
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4357..4736
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4737..4940
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4891..4911
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4937..4961
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4989..4998
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5073..5096
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    6397..6531
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6421..6440)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6475..6494
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6674..6696)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7256..9045
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gggattctcg ggacccctcc tcttcctctt caaggtcacc agacagagat gctactgggg
       61 caacggaaga aaagctgggt gcggcctgtg aggatcagct tatcgatgat aagctgtcaa
      121 acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
      181 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
      241 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      301 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      361 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      421 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      481 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      541 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
      601 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      661 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      721 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
      781 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
      841 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
      901 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
      961 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1021 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1081 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1141 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1201 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1261 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1321 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1381 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1441 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1501 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1561 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1621 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1681 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1741 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1801 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1861 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1921 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1981 acggttcctg gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct
     2041 ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
     2101 ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc
     2161 cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa
     2221 gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg
     2281 gtgaatccgt tagcgaggtg ccgccctgct tcatccccgt ggcccgttgc tcgcgtttgc
     2341 tggcggtgtc cccggaagaa atatatttgc atgtctttag ttctatgatg acacaaaccc
     2401 cgcccagcgt cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc
     2461 cgaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca
     2521 gcgacccgct taacagcgtc aacagcgtgc cgcagatccc ggggggcaat gagatatgaa
     2581 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     2641 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     2701 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     2761 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     2821 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     2881 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     2941 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     3001 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     3061 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     3121 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     3181 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     3241 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     3301 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     3361 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     3421 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     3481 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     3541 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccggatc gggagatggg
     3601 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     3661 taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg
     3721 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     3781 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     3841 aacgtcgggg cggcaggccc tgccatagcc actggccccg tgggttaggg acggggtccc
     3901 ccatggggaa tggtttatgg ttcgtggggg ttattatttt gggcgttgcg tggggtcagg
     3961 tccacgactg gactgagcag acagacccat ggtttttgga tggcctgggc atggaccgca
     4021 tgtactggcg cgacacgaac accgggcgtc tgtggctgcc aaacaccccc gacccccaaa
     4081 aaccaccgcg cggatttctg gcgtgccaag ctagtcgacc aattctcatg tttgacagct
     4141 tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat
     4201 ggaagatcta tacattgaat caatattggc aattagccat attagtcatt ggttatatag
     4261 cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca
     4321 tttatattgg ctcatgtcca atatgaccgc catgttgaca ttgattattg actagttatt
     4381 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     4441 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     4501 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     4561 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc
     4621 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     4681 tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     4741 tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     4801 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     4861 caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg
     4921 aggtctatat aagcagagct cgtttagtga accgtcagat ctctagaagc tgggtaccag
     4981 ctgctagcgc caccatggcc gtcatggcgc cccgaaccct cgtcctgcta ctctcggggg
     5041 ccctggccct gacccagacc tgggcgggct ccgactacaa ggacgatgat gacaagggag
     5101 gatccaagcc ccacccagca gaggggcagt ggcgggcagt ggacgtggtc ctagactgtt
     5161 tcctggtgaa ggacggtgcg caccgtggag ctctcgccag cagtgaggac agggcaaggg
     5221 cctcccttgt gctgaagcag gtgccagtgc tggacgatgg ctccctggag gacttcaccg
     5281 atttccaagg gggcacactg gcccaagatg acccacctat tatctttgag gcctcagtgg
     5341 acctggtcca gattccccag gccgaggcct tgctccatgc tgactgcagt gggaaggagg
     5401 tgacctgtga gatctcccgc tactttctcc agatgacaga gaccactgtt aagacagcag
     5461 cttggttcat ggccaacgtg caggtctctg gagggggacc tagcatctcc ttggtgatga
     5521 agactcccag ggtcgccaag aatgaggtgc tctggcaccc aacgctgaac ttgccactga
     5581 gcccccaggg gactgtgcga actgcagtgg agttccaggt gatgacacag acccaatccc
     5641 tgagcttcct gctggggtcc tcagcctcct tggactgtgg cttctccatg gcaccgggct
     5701 tggacctcat cagtgtgaag tgggaactga gccacaaggg caggggtcag ttggtgtaca
     5761 gctggaccgc agggcagggg caggctgtgc ggaagggcgc taccctggag cctgcacaac
     5821 tgggcatggc cagggatgcc tccctcaccc tgcccggcct cactatacag gacgagggga
     5881 cctacatttg ccagatcacc acctctctgt accgagctag ccagatcatc cagctcaaca
     5941 tccaagcttc ccctaaagta cgactgagct tggcaaacga agctctgctg cccaccctca
     6001 tctgcgacat tgctggctat taccctctgg atgtggtggt gacgtggacc cgagaggagc
     6061 tgggtggatc cccagcccaa gtctctggtg cctccttctc cagcctcagg caaagcgtgg
     6121 caggcaccta cagcatctcc tcctctctca ccgcagaacc tggctctgca ggtgccactt
     6181 acacctgcca ggtcacacac atctctctgg aggagcccct tggggccagc acccaggttg
     6241 tcccaccaga gcggagatct ggagccggaa gtgctatccc catcatgggt atcgttgctg
     6301 gcctggttgt ccttgcagct gtagtcactg gagctgcggt cgctgctgtg ctgtggagaa
     6361 agaagagctc agattgactc gaggccggca aggccggatc cagacatgat aagatacatt
     6421 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     6481 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     6541 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     6601 taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg
     6661 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     6721 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     6781 gcgcagccat gaggtcgact ctagaggatc gatccccgcc ccggacgaac taaacctgac
     6841 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     6901 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     6961 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     7021 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     7081 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     7141 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     7201 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     7261 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     7321 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     7381 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     7441 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     7501 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     7561 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     7621 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     7681 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     7741 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     7801 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     7861 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     7921 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     7981 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     8041 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     8101 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     8161 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     8221 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     8281 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     8341 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     8401 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     8461 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     8521 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     8581 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     8641 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     8701 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     8761 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     8821 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     8881 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgcat
     8941 atacagtcag catatgatac ccagtagtag agtgggagtg ctatcctttg catatgccgc
     9001 cacctcccaa gggggcgtga attttcgctg cttgtccttt tcctgctggt tgctcccatt
     9061 cttaggtgaa tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta
     9121 aatgtcgcta atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca
     9181 acatgggtat gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc
     9241 cagaaataca ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga
     9301 atacacggct tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt
     9361 cctcaccctc atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg
     9421 cagccccttc caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc
     9481 acacagtcac cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg
     9541 catccttcaa aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa
     9601 tggactccct tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac
     9661 gactcaatgg tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt
     9721 aaagggaggt cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg
     9781 tagtcctttc tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa
     9841 atttcgggtt ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc
     9901 tgcctccatc accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg
     9961 ggccctgctc tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt
    10021 ggtattcaaa ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg
    10081 gcctgcgcgg tggagacccg gatgatgatg actgactact gggactcctg ggcctctttt
    10141 ctccacgtcc acgacctctc cccctggctc tttcacgact tccccccctg gctctttcac
    10201 gtcctctacc ccggcggcct ccactacctc ctcgaccccg gcctccacta cctcctcgac
    10261 cccggcctcc actgcctcct cgaccccggc ctccacctcc tgctcctgcc cctcctgctc
    10321 ctgcccctcc tcctgctcct gcccctcctg cccctcctsc tcctscccct cctscccctc
    10381 ctsctcctsc ccctccyccc ccycctccyc cyccccctcc ycccccyccy cctccyccyc
    10441 cyccycctcc ccctcctcct cctcctcccc ctcctccccc tcctcctcct ccccctccts
    10501 cccctcctsc tcctscccct cctgcccctc ctgctcctgc ccctcctgct cctscccctc
    10561 ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
    10621 cccctcctgc tcctgcccct cctgcccctc ctgcccctcc tgctcctgcc cctcctcctg
    10681 ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc
    10741 ctsctcctgc ccctcctcct sctcctsccc ctcctscycc tcctscyccy cctcctcctc
    10801 ctccycctcc tccycctcct cctsctccts cycctcctcc tsctcctscc cctcctscyc
    10861 ctcctscccc tcctcctsct cctscccctc ctcctsctcc tscccctcct gcccctcctg
    10921 cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgctcctg
    10981 cccctcccgc tcctgctcct gctcctgttc caccgtgggt ccctttgcag ccaatgcaac
    11041 ttggacgttt ttggggtctc cggacaccat ctctatgtct tggccctgat cctgagccgc
    11101 ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt
    11161 tatcaccccc tcttctttga ggtccactgc cgccggagcc ttctggtcca gatgtgtctc
    11221 ccttctctcc taggccattt ccaggtcctg tacctggccc ctcgtcagac atgattcaca
    11281 ctaaaagaga tcaatagaca tctttattag acgacgctca gtgaatacag ggagtgcaga
    11341 ctcctgcccc ctccaacagc ccccccaccc tcatcccctt catggtcgct gtcagacaga
    11401 tccaggtctg aaaattcccc atcctccgaa ccatcctcgt cctcatcacc aattactcgc
    11461 agcccggaaa actcccgctg aacatcctca agatttgcgt cctgagcctc aagccaggcc
    11521 tcaaattcct cgtccccctt tttgctggac ggtagggatg
//
