LOCUS       sequence-354781-       11248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged MR1 (H83P).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   McShan AC, Devlin CA, Papadaki GF, Sun Y, Green AI, Morozov GI, 
            Burslem GM, Procko E, Sgourakis NG
  TITLE     TAPBPR employs a ligand-independent docking mechanism to chaperone 
            MR1 molecules.
  JOURNAL   Nat Chem Biol. 2022 Jun 20. pii: 10.1038/s41589-022-01049-9. doi: 
            10.1038/s41589-022-01049-9.
  PUBMED    35725941
REFERENCE   2  (bases 1 to 11248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 Jun 20. pii: 10.1038/s41589-022-01049-9. doi: 
            10.1038/s41589-022-01049-9."
COMMENT     Sequence Label: pCEP4 MR1 (H83P)
FEATURES             Location/Qualifiers
     source          1..11248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(147..165)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        233..337
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(338..406,407..1198)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(556..575)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1369..1957
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1858..1877
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2388..2533
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2576..3613
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3611..3630)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3655..3703
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4357..4736
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4737..4940
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4891..4911
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4937..4961
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5043..5072
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(5839..5850)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6085..6219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6109..6128)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6163..6182
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6362..6384)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6944..8733
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(9035..10960)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGXGGGXGGXGGXGXGGGXGXGGGXGGXGXGGGX
                     GGXGGGGXGGGXGXGGGXGGXGXGGGXGGXGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGXGGXGXGGXGGXGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
ORIGIN
        1 gggattctcg ggacccctcc tcttcctctt caaggtcacc agacagagat gctactgggg
       61 caacggaaga aaagctgggt gcggcctgtg aggatcagct tatcgatgat aagctgtcaa
      121 acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
      181 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
      241 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      301 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      361 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      421 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      481 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      541 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
      601 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      661 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      721 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
      781 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
      841 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
      901 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
      961 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1021 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1081 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1141 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1201 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1261 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1321 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1381 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1441 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1501 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1561 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1621 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1681 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1741 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1801 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1861 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1921 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1981 acggttcctg gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct
     2041 ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
     2101 ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc
     2161 cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa
     2221 gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg
     2281 gtgaatccgt tagcgaggtg ccgccctgct tcatccccgt ggcccgttgc tcgcgtttgc
     2341 tggcggtgtc cccggaagaa atatatttgc atgtctttag ttctatgatg acacaaaccc
     2401 cgcccagcgt cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc
     2461 cgaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca
     2521 gcgacccgct taacagcgtc aacagcgtgc cgcagatccc ggggggcaat gagatatgaa
     2581 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     2641 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     2701 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     2761 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     2821 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     2881 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     2941 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     3001 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     3061 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     3121 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     3181 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     3241 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     3301 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     3361 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     3421 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     3481 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     3541 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccggatc gggagatggg
     3601 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     3661 taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg
     3721 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     3781 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     3841 aacgtcgggg cggcaggccc tgccatagcc actggccccg tgggttaggg acggggtccc
     3901 ccatggggaa tggtttatgg ttcgtggggg ttattatttt gggcgttgcg tggggtcagg
     3961 tccacgactg gactgagcag acagacccat ggtttttgga tggcctgggc atggaccgca
     4021 tgtactggcg cgacacgaac accgggcgtc tgtggctgcc aaacaccccc gacccccaaa
     4081 aaccaccgcg cggatttctg gcgtgccaag ctagtcgacc aattctcatg tttgacagct
     4141 tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat
     4201 ggaagatcta tacattgaat caatattggc aattagccat attagtcatt ggttatatag
     4261 cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca
     4321 tttatattgg ctcatgtcca atatgaccgc catgttgaca ttgattattg actagttatt
     4381 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     4441 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     4501 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     4561 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc
     4621 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     4681 tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     4741 tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     4801 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     4861 caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg
     4921 aggtctatat aagcagagct cgtttagtga accgtcagat ctctagaagc tgggtaccag
     4981 ctgctagcgc caccatgaag accatcatcg ccctgagcta catcttctgc ctggtgttcg
     5041 ccgagcagaa attgatcagc gaggaggacc tgggcagccc tggaggcagt tcaggaggct
     5101 ctcgtaccca ttcccttcgt tactttcgcc ttggtgtatc agatcctatc cacggtgtcc
     5161 ccgaatttat ctctgtagga tacgttgact cacatcccat cactacatat gacagcgtca
     5221 cacgccagaa ggagccaagg gctccatgga tggccgagaa cttggcacca gatcattggg
     5281 aacgctatac acaactgttg cgcggctggc aacaaatgtt caaagttgaa ctcaaaagac
     5341 tccaacgccc ctataatcat agtggatcac atacttatca gcgaatgata ggttgtgagc
     5401 tgcttgagga tggaagcacc actgggttcc tgcagtacgc ttatgatggt caggactttc
     5461 tcatattcaa caaagacacc cttagttggc ttgctgttga caacgttgca cacaccatta
     5521 aacaagcctg ggaagccaac cagcacgaac tgttgtacca aaagaactgg cttgaggagg
     5581 agtgcatcgc ctggttgaaa cggttcctcg aatatggcaa ggacacactt caacgcaccg
     5641 aacccccact tgtcagggtg aatcggaagg aaactttccc aggtgtcact gcactcttct
     5701 gtaaggccca tggtttctat cctcctgaaa tctacatgac ttggatgaag aacggtgaag
     5761 aaatagtgca agagatcgat tacggcgata ttcttcccag cggggacggt acttatcaag
     5821 catgggcctc catagaactc gaccctcaat ctagcaactt gtatagttgc cacgtcgaac
     5881 attgcggcgt acacatggta cttcaggtcc ctcaagagtc agaaactatt ccactggtta
     5941 tgaaagctgt ttccgggagt attgttttgg tcattgtcct cgctggagta ggggtattgg
     6001 tttggcgcag acgaccccgg gaacagaatg gagcaatcta tttgccgacg cccgaccggt
     6061 aatgactcga ggccggcaag gccggatcca gacatgataa gatacattga tgagtttgga
     6121 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     6181 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     6241 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     6301 aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa
     6361 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     6421 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     6481 ggtcgactct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     6541 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     6601 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     6661 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     6721 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     6781 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     6841 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     6901 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     6961 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     7021 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     7081 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     7141 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     7201 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     7261 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     7321 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     7381 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     7441 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     7501 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     7561 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     7621 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     7681 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     7741 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     7801 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     7861 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     7921 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     7981 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     8041 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     8101 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     8161 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     8221 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     8281 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     8341 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     8401 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     8461 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     8521 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     8581 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgcatat acagtcagca
     8641 tatgataccc agtagtagag tgggagtgct atcctttgca tatgccgcca cctcccaagg
     8701 gggcgtgaat tttcgctgct tgtccttttc ctgctggttg ctcccattct taggtgaatt
     8761 taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat
     8821 gttttccaac gcgagaaggt gttgagcgcg gagctgagtg acgtgacaac atgggtatgc
     8881 ccaattgccc catgttggga ggacgaaaat ggtgacaaga cagatggcca gaaatacacc
     8941 aacagcacgc atgatgtcta ctggggattt attctttagt gcgggggaat acacggcttt
     9001 taatacgatt gagggcgtct cctaacaagt tacatcactc ctgcccttcc tcaccctcat
     9061 ctccatcacc tccttcatct ccgtcatctc cgtcatcacc ctccgcggca gccccttcca
     9121 ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc
     9181 tgatattgca ggtaggagcg ggctttgtca taacaaggtc cttaatcgca tccttcaaaa
     9241 cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata acagacaatg gactccctta
     9301 gcgggccagg ttgtgggccg ggtccagggg ccattccaaa ggggagacga ctcaatggtg
     9361 taagacgaca ttgtggaata gcaagggcag ttcctcgcct taggttgtaa agggaggtct
     9421 tactacctcc atatacgaac acaccggcga cccaagttcc ttcgtcggta gtcctttcta
     9481 cgtgactcct agccaggaga gctcttaaac cttctgcaat gttctcaaat ttcgggttgg
     9541 aacctccttg accacgatgc tttccaaacc accctccttt tttgcgcctg cctccatcac
     9601 cctgaccccg gggtccagtg cttgggcctt ctcctgggtc atctgcgggg ccctgctcta
     9661 tcgctcccgg gggcacgtca ggctcaccat ctgggccacc ttcttggtgg tattcaaaat
     9721 aatcggcttc ccctacaggg tggaaaaatg gccttctacc tggagggggc ctgcgcggtg
     9781 gagacccgga tgatgatgac tgactactgg gactcctggg cctcttttct ccacgtccac
     9841 gacctctccc cctggctctt tcacgacttc cccccctggc tctttcacgt cctctacccc
     9901 ggcggcctcc actacctcct cgaccccggc ctccactacc tcctcgaccc cggcctccac
     9961 tgcctcctcg accccggcct ccacctcctg ctcctgcccc tcctgctcct gcccctcctc
    10021 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct gctcctsccc
    10081 ctcctscccc tcctsctcct scccctccts cccctcctcc tgctcctgcc cctcctgccc
    10141 ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc
    10201 ctgcccctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gctcctgccc
    10261 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgctc
    10321 ctgcccctcc tgcccctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc
    10381 ctscccctcc tscccctcct cctsctccts cccctcctsc ccctcctcct sctcctsccc
    10441 ctcctcctsc tcctccccct cctscccctc ctscccctcc tcctsctcct scccctccts
    10501 cccctcctcc tsctcctscc cctcctccts ctcctscccc tcctscccct cctscccctc
    10561 ctcctsctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctgccc
    10621 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc cctcccgctc
    10681 ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt
    10741 ggggtctccg gacaccatct ctatgtcttg gccctgatcc tgagccgccc ggggctcctg
    10801 gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta tcaccccctc
    10861 ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc ttctctccta
    10921 ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact aaaagagatc
    10981 aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact cctgccccct
    11041 ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc caggtctgaa
    11101 aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac
    11161 tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg
    11221 tccccctttt tgctggacgg tagggatg
//
