LOCUS       sequence-354786-        9355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express iRFP with early endosome-targeting sequence in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9355)
  AUTHORS   Kashiwagi S, Fujioka Y, Satoh AO, Yoshida A, Fujioka M, Nepal P, 
            Tsuzuki A, Aoki O, Paudel S, Sasajima H, Ohba Y
  TITLE     Folding Latency of Fluorescent Proteins Affects the Mitochondrial 
            Localization of Fusion Proteins.
  JOURNAL   Cell Struct Funct. 2019 Dec 26;44(2):183-194. doi: 
            10.1247/csf.19028. Epub 2019 Nov 15.
  PUBMED    31735740
REFERENCE   2  (bases 1 to 9355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1247/csf.19028";
            journalName: "Cell Struct Funct"; date: "2019-12-26- 26"; volume: 
            "44"; issue: "2"; pages: "183-194"
COMMENT     Sequence Label: pFX-iRFP-EEA1
FEATURES             Location/Qualifiers
     source          1..9355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1608..5843
                     /codon_start=1
                     /product="human early endosome antigen 1"
                     /label=EEA1
                     /translation="MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQ
                     CMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKW
                     YSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQ
                     KAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIED
                     VAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQ
                     EVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLH
                     QKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQ
                     QQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADL
                     QLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQ
                     IGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQ
                     VTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKE
                     KVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVT
                     AKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDL
                     QQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKQIL
                     KQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKN
                     SKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLK
                     LELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQK
                     TELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQD
                     LKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQD
                     IQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQ
                     LLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEK
                     EVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEAT
                     VQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQ
                     ALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVC
                     DACFNDLQG"
     polyA_signal    6143..6264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6180..6199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6234..6253
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6271..6726)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6408..6429)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6620..6639
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6753..6857
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6859..7216
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6859..7050
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6882..6902)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7067..7202
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7251..8045
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7251..8045
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7305..7324)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7915..7934
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8233..8252)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8277..8324
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8653..9241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9142..9161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggttctag aagtactgaa ttcaccatgg ccgaagggag cgtggcaaga cagcccgatc
      661 tgctgacatg cgacgacgaa ccaatccaca ttccaggagc tatccagcct catggactgc
      721 tgctggctct ggccgctgac atgactatcg tggcaggctc tgataacctg ccagagctga
      781 ccgggctggc tatcggagca ctgattggcc ggtccgcagc cgacgtgttc gattctgaga
      841 ctcacaatag actgaccatc gccctggctg aacctggggc tgcagtggga gccccaatta
      901 ccgtcggctt cacaatgcga aaggacgctg gctttatcgg gtcctggcac cgccatgatc
      961 agctgatttt tctggagctg gaaccacctc agagagacgt ggcagagcca caggctttct
     1021 ttcggagaac aaacagcgcc atcaggcgcc tgcaggccgc tgagactctg gaatccgctt
     1081 gcgcagccgc tgcacaggaa gtgaggaaga tcaccgggtt cgatcgcgtc atgatctaca
     1141 gattcgcctc agactttagc ggagaagtga tcgcagaaga tcgctgtgcc gaggtcgaaa
     1201 gtaaactggg cctgcattac ccagcctcaa ccgtgcctgc acaggcacga cggctgtata
     1261 caatcaaccc cgtgcggatc attcccgaca ttaattacag accagtgccc gtcacacctg
     1321 acctgaaccc agtgactggg aggcccatcg atctgagctt cgccattctg cgcagcgtgt
     1381 cccctgtcca cctggagttt atgaggaata tcggaatgca tggcaccatg tctatcagta
     1441 ttctgagagg cgaaaggctg tgggggctga tcgtgtgcca ccatcggaca ccttactatg
     1501 tggacctgga cggcaggcag gcttgtgaac tggtggctca ggtgctggct tggcagattg
     1561 gcgtgatgga agaaagatct ggaggatccg gaggactcga gcccaccatg ttaaggagga
     1621 ttttacagag gactcctggg agagttggct ctcaaggttc tgatttagat tcatcagcaa
     1681 ctcctataaa cacagtggac gtcaataatg aaagctcttc agaaggtttc atatgtcccc
     1741 agtgtatgaa atctcttgga tctgctgatg aacttttcaa acattatgaa gctgttcatg
     1801 atgctggtaa tgactcaggt catggaggag agtctaatct tgctttgaag cgagatgatg
     1861 taacactgct cagacaagag gtccaagacc tacaggcttc acttaaggaa gaaaaatggt
     1921 actcggaaga attaaagaag gaattagaaa aatatcaagg gctgcagcag caagaggcca
     1981 aacctgatgg gttggtgact gattcatcag cagaactaca gtctttggaa cagcaattag
     2041 aagaagccca aacagaaaat tttaatatta agcaaatgaa agacttattt gaacagaaag
     2101 cagcccaact tgctactgaa attgcagata taaagtcaaa gtatgatgaa gaaaggagtc
     2161 ttcgagaagc tgctgaacaa aaagtgacac gtctgacaga agaattaaac aaagaggcaa
     2221 ctgtaattca agatctgaag acggaactgc ttcagagacc tggtatagaa gatgttgccg
     2281 tgctaaagaa agaactggtc caagttcaaa cactaatgga taacatgacc ttggaacgtg
     2341 agcgagaatc tgaaaaactc aaagatgaat gcaaaaaatt gcagtcacaa tatgctagct
     2401 cagaggccac aataagccag ctaaggagtg aacttgccaa aggcccccag gaagttgctg
     2461 tatatgtaca ggaactacaa aaactgaaaa gttcagttaa tgaattaaca caaaaaaatc
     2521 agaccttgac agaaaacttg ctgaaaaaag aacaagacta tactaagtta gaggagaaac
     2581 ataatgaaga atctgtgagt aaaaagaata ttcaggcaac ccttcatcaa aaagacctag
     2641 attgtcaaca gcttcagtca agattgtctg catctgaaac ctcactgcat agaatacatg
     2701 tagaactaag tgaaaaagga gaagctactc aaaagctcaa agaagaatta tctgaggtag
     2761 agaccaagta ccagcatcta aaggcggagt ttaagcagct acaacaacag agagaagaaa
     2821 aggagcagca tgggttacaa ctccaaagtg aaattaatca attacatagc aaacttctag
     2881 agacagagcg ccaactaggg gaagctcatg gtaggctgaa ggaacagaga cagctttcaa
     2941 gtgaaaagtt gatggataaa gaacaacaag tggctgattt acaactcaaa ctttctcggt
     3001 tagaagagca gttgaaggaa aaagttacaa attctacaga attgcagcat caattagata
     3061 aaacaaagca acagcatcaa gaacaacagg ctcttcagca aagcaccacg gcaaaacttc
     3121 gagaagctca gaatgatttg gaacaagttc tacgtcaaat tggcgataag gaccaaaaga
     3181 tccagaacct tgaagcttta ttacagaaga gtaaagaaaa tatttcatta ctagaaaaag
     3241 aaagagaaga tctttatgca aaaattcagg ctggtgaagg agagactgct gttcttaacc
     3301 agttacaaga aaaaaaccat acactacagg agcaagtaac tcaactaaca gagaagctga
     3361 agaatcagtc agaaagtcat aaacaagccc aggagaattt gcatgaccag gtacaagagc
     3421 agaaggcaca tcttagagct gcacaagacc gtgtcctttc cctagaaact agtgtcaatg
     3481 aattaaatag tcaattaaat gaaagcaagg agaaggtctc ccagcttgac atacagatta
     3541 aagccaaaac cgaactatta ctatcagcag aagcagcaaa aactgctcaa agagctgatc
     3601 ttcagaatca tttggacaca gctcaaaatg cattacaaga taaacagcag gagttaaata
     3661 agattactac tcagttggat caggtcactg caaagttaca agacaagcaa gaacattgca
     3721 gtcagctgga aagtcatctt aaagaatata aagagaaata cctctcttta gaacagaaaa
     3781 ccgaagagct agaaggtcaa attaagaaac tagaagctga tagtcttgaa gttaaagcaa
     3841 gcaaggagca ggctttgcaa gatctacaac agcaaagaca gctgaacaca gatttagagc
     3901 tcagagccac agaattgagt aaacaacttg aaatggagaa ggaaatagta tccagtacaa
     3961 gattggatct acagaaaaaa tctgaagccc ttgaaagtat caagcaaaag cttaccaagc
     4021 aagaggaaga aaaacaaatc ctgaaacaag attttgaaac tttaagtcaa gaaacaaaga
     4081 ttcagcatga ggaattgaat aacagaattc aaacaacagt aacagaacta caaaaagtga
     4141 aaatggagaa agaagcttta atgacagagc tttctacagt aaaggacaaa ctatcaaaag
     4201 tttctgattc tttgaaaaac tctaaaagtg aatttgaaaa ggagaatcag aaaggaaaag
     4261 ccgctatatt agacttggaa aaaacttgca aagaattaaa gcatcaactt caagtgcaga
     4321 tggaaaacac acttaaggaa cagaaggaac tgaaaaagtc acttgaaaaa gagaaggagg
     4381 cttctcatca gttgaaattg gaactcaatt caatgcagga acaacttata caggcccaga
     4441 atactttaaa acaaaatgaa aaagaagagc aacaacttca ggggaacata aatgagctaa
     4501 agcaatcaag tgaacagaag aaaaaacaaa ttgaagcact ccaaggagag cttaaaattg
     4561 ctgttttaca gaagacagag cttgagaata aactacagca gcagttaaca caggcagccc
     4621 aggaacttgc agcagagaaa gagaaaatat cagtattaca aaacaactat gaaaaaagtc
     4681 aggaaacttt caaacagctt caatctgatt tctatgggag ggaatctgaa cttctagcca
     4741 ccaggcaaga tcttaagtct gtagaagaga agctttctct agcacaggag gacttgattt
     4801 caaacagaaa tcaaattgga aatcaaaata aattgattca agaactgaag actgccaagg
     4861 ctacattgga gcaggattca gcaaagaaag aacagcaatt gcaggagcga tgtaaagcac
     4921 tacaagacat tcagaaagaa aagtcactga aagaaaaaga actggtaaat gagaagtcta
     4981 aattggcaga gatagaagaa attaaatgta gacaagaaaa agaaatcact aaactaaacg
     5041 aagaactcaa gtcccacaaa ctagaaagca taaaggagat aacaaatctt aaagatgcta
     5101 aacagcttct aattcagcag aaattagaac ttcaaggaaa agcggactcc ctgaaggcag
     5161 ctgttgaaca ggagaagaga aatcagcaga tactaaaaga ccaggtgaaa aaggaagaag
     5221 aggagctgaa gaaagaattt attgagaaag aagctaagtt gcattccgaa ataaaagaaa
     5281 aggaagtagg aatgaagaag catgaagaaa atgaggctaa acttaccatg cagattacag
     5341 cattaaatga aaacttaggc actgtgaaga aggagtggca atctagtcaa cggagagtta
     5401 gtgagcttga gaaacaaacg gatgacttac ggggtgaaat tgcagtatta gaagcaacgg
     5461 ttcagaataa tcaagatgaa aggagagcac tactggaaag atgtcttaaa ggagaaggtg
     5521 aaatagaaaa gcttcaaacc aaagtattag aattgcaaag aaagctggat aatacaactg
     5581 cagcagtgca ggagctgggc agagaaaacc aatcacttca gatcaaacat acacaagcgt
     5641 tgaatagaaa gtgggccgaa gacaatgaag tacaaaactg tatggcctgt gggaaaggct
     5701 tttcagtaac agtgagacgg catcactgcc gacagtgtgg aaatatcttc tgtgctgaat
     5761 gttcagccaa aaatgcctta actccttcct ccaagaagcc tgttcgtgtc tgtgatgcat
     5821 gtttcaatga cttgcaagga taatgggtta tcacaacttc agagtaatat tacactaaca
     5881 ttagattttt aataaatgta cttaatagag gtcttggact actatttggt ttggacactg
     5941 gttgcaatac tagaccaaat aggaattagt atattttgga atgttatgga tatcgcggcc
     6001 gctgactgac tgaagatcca ccggatctag ataactgatc ataatcagcc ataccacatt
     6061 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     6121 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     6181 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6241 gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat
     6301 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     6361 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     6421 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     6481 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     6541 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     6601 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     6661 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     6721 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6781 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6841 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     6901 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6961 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7021 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     7081 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7141 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7201 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     7261 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     7321 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     7381 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     7441 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     7501 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     7561 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     7621 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     7681 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     7741 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     7801 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     7861 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     7921 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     7981 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     8041 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     8101 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     8161 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
     8221 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     8281 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
     8341 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     8401 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     8461 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
     8521 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8581 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8641 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8701 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8761 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8821 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8881 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8941 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9001 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9061 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9121 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9181 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9241 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9301 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
