LOCUS       sequence-354813-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LECT2 I40 variant protein from T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Belonogov L, Taylor PE, Wong S, Morgan GJ
  TITLE     An amyloidosis-associated polymorphism does not alter LECT2 
            stability in vitro
  JOURNAL   bioRxiv 2022.03.01.482540
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.03.01.482540"
COMMENT     Sequence Label: pET-11-LECT2-I40
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(500..518)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      514..561
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(736..741,742..758,759..764))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(791..809)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctagaataat tttgtttaac tttaagaagg agatatacat atggggccat gggctaatat
       61 atgtgctggc aagtcttcca atgagatccg gacgtgtgac cgccatggct gtggacagta
      121 ctctgctcaa agaagtcaga ggcctcacca gggtgtggac atcttgtgct ctgctggatc
      181 tactgtgtac gcaccattca ctggaatgat tgtgggccag gagaaacctt atcaaaacaa
      241 gaatgctatc aataatggtg ttcgaatatc tggaagaggt ttttgtgtca aaatgttcta
      301 cattaagcca attaagtata aaggtcctat taagaaggga gaaaaacttg gaactctatt
      361 gcccttgcag aaagtttatc ctggcataca atcgcatgtg cacattgaaa actgtgactc
      421 gagtgaccct actgcatacc tgtagaatag gatccggctg ctaacaaagc ccgaaaggaa
      481 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      541 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc cgcaagaggc
      601 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga
      661 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact
      721 gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga
      781 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatga
//
