LOCUS       sequence-35483-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  TITLE     Horwitz lab unpublished plasmids
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Reduced Expression Alpha-5-Integrin-GFP
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga tggatcccgg gcccgcggta ccggcatcag
       61 aggtggctgg aggcttgagc tgagcttttt ccatggcggt gccatatggg agggagcgtt
      121 tgaagaatcc aagcttgtag aggatgtaga tgagtagacc taggagcagg aggccaaaca
      181 ggatggctag gatgatgatc cacagtggga cgccatagct gccttctgcc ttggtccatt
      241 gcacagctgt ggccacctga cgctcttttt ggggcagctg ccgaggcagg attcggtagg
      301 gcatcttcag ggctttgtac acagcctcac actgcaggct aaatggctgg tgctcccgct
      361 gcaagaaagt cttggcccag actcggaaat gcaactgcag actttggctc tcttgttggt
      421 gcaggggccc gagctcacag cgcagcctga aacactcagc ctccgggcat ttcaggatct
      481 gaggtcccga ggaagcagag ctgcggcttg gagcttcccg tttttgctgg tggtgcaggg
      541 aaccctcggg atccaactcc aggccctttg ggttaatggg gtgattggtg gtgcagttga
      601 gtcccgtaac tctggtcaca tataggagct gctgaccttc cagagcctgg ggacagctga
      661 gttccagcac accctggcta atggagctgg ggccttggtt gatgagctca tagacatggt
      721 ggacagcagg tcccaggtcc tcctccttct ga
//
