LOCUS       sequence-354841-        8084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA 
            scaffold seqeuence has been modified for increased sgRNA expression 
            (v3)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Chey YCJ, Gierus L, Lushington C, Arudkumar JC, B Geiger A, Staker 
            LG, Robertson LJ, Pfitzner C, Kennedy JG, Lee RHB, Godahewa GI, 
            Adikusuma F, Thomas PQ
  TITLE     Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA 
            transcript levels through scaffold poly-T tract reduction.
  JOURNAL   BMC Genomics. 2025 Feb 12;26(1):138. doi: 
            10.1186/s12864-025-11317-2.
  PUBMED    39939860
REFERENCE   2  (bases 1 to 8084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2025-02-12"; volume: "26(1):138. doi"; pages: " 
            10.1186/s12864-025-11317-2"
COMMENT     Sequence Label: SaCas9v3-Puro
FEATURES             Location/Qualifiers
     source          1..8084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        268..343
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     enhancer        364..649
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        651..928
                     /label=chicken beta-actin promoter
     intron          929..1156
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1168..1177
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1180..1200
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1225..4380
                     /codon_start=1
                     /product="Cas9 endonuclease from the Staphylococcus aureus 
                     Type II CRISPR/Cas system"
                     /label=SaCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG
                     RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF
                     SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV
                     RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW
                     KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ
                     IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI
                     IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI
                     NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK
                     VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK
                     YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ
                     EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS
                     VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN
                     KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF
                     ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN
                     DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD
                     NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL
                     DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN
                     RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
                     IKKG"
     CDS             4381..4428
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4444..4497
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4498..5097
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4498..4517)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4994..5014
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5125..5142)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5131..5338
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5347..5487
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5347..5476
                     /label=AAV2 ITR
     rep_origin      5562..6017
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5649..5668)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5859..5880
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6034..6053)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6153..6175
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6213..6231)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6299..6403
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6404..6472,6473..7264)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6622..6641)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7435..8023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7924..7943
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg ggtcttcgag aagacctgtt tcagtactct ggaaacagaa tctactgaaa
      301 caaggcaaaa tgccgtgttt atctcgtcaa cttgttggcg agattttttg cggccgcggt
      361 acccgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      421 attgacgtca atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      481 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      541 tgacgtcaat gacggtaaat ggcccgcctg gcattgtgcc cagtacatga ccttatggga
      601 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
      661 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
      721 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc
      781 rggsggggsg gggsggggsg rggggsgggg sggggcgagg cggagaggtg cggcggcagc
      841 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
      901 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
      961 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     1021 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagctgagca agaggtaagg
     1081 gtttaaggga tggttggttg gtggggtatt aatgtttaat tacctggagc acctgcctga
     1141 aatcactttt tttcaggttg gaccggtgcc accatggccc caaagaagaa gcggaaggtc
     1201 ggtatccacg gagtcccagc agccaagcgg aactacatcc tgggcctgga catcggcatc
     1261 accagcgtgg gctacggcat catcgactac gagacacggg acgtgatcga tgccggcgtg
     1321 cggctgttca aagaggccaa cgtggaaaac aacgagggca ggcggagcaa gagaggcgcc
     1381 agaaggctga agcggcggag gcggcataga atccagagag tgaagaagct gctgttcgac
     1441 tacaacctgc tgaccgacca cagcgagctg agcggcatca acccctacga ggccagagtg
     1501 aagggcctga gccagaagct gagcgaggaa gagttctctg ccgccctgct gcacctggcc
     1561 aagagaagag gcgtgcacaa cgtgaacgag gtggaagagg acaccggcaa cgagctgtcc
     1621 accaaagagc agatcagccg gaacagcaag gccctggaag agaaatacgt ggccgaactg
     1681 cagctggaac ggctgaagaa agacggcgaa gtgcggggca gcatcaacag attcaagacc
     1741 agcgactacg tgaaagaagc caaacagctg ctgaaggtgc agaaggccta ccaccagctg
     1801 gaccagagct tcatcgacac ctacatcgac ctgctggaaa cccggcggac ctactatgag
     1861 ggacctggcg agggcagccc cttcggctgg aaggacatca aagaatggta cgagatgctg
     1921 atgggccact gcacctactt ccccgaggaa ctgcggagcg tgaagtacgc ctacaacgcc
     1981 gacctgtaca acgccctgaa cgacctgaac aatctcgtga tcaccaggga cgagaacgag
     2041 aagctggaat attacgagaa gttccagatc atcgagaacg tgttcaagca gaagaagaag
     2101 cccaccctga agcagatcgc caaagaaatc ctcgtgaacg aagaggatat taagggctac
     2161 agagtgacca gcaccggcaa gcccgagttc accaacctga aggtgtacca cgacatcaag
     2221 gacattaccg cccggaaaga gattattgag aacgccgagc tgctggatca gattgccaag
     2281 atcctgacca tctaccagag cagcgaggac atccaggaag aactgaccaa tctgaactcc
     2341 gagctgaccc aggaagagat cgagcagatc tctaatctga agggctatac cggcacccac
     2401 aacctgagcc tgaaggccat caacctgatc ctggacgagc tgtggcacac caacgacaac
     2461 cagatcgcta tcttcaaccg gctgaagctg gtgcccaaga aggtggacct gtcccagcag
     2521 aaagagatcc ccaccaccct ggtggacgac ttcatcctga gccccgtcgt gaagagaagc
     2581 ttcatccaga gcatcaaagt gatcaacgcc atcatcaaga agtacggcct gcccaacgac
     2641 atcattatcg agctggcccg cgagaagaac tccaaggacg cccagaaaat gatcaacgag
     2701 atgcagaagc ggaaccggca gaccaacgag cggatcgagg aaatcatccg gaccaccggc
     2761 aaagagaacg ccaagtacct gatcgagaag atcaagctgc acgacatgca ggaaggcaag
     2821 tgcctgtaca gcctggaagc catccctctg gaagatctgc tgaacaaccc cttcaactat
     2881 gaggtggacc acatcatccc cagaagcgtg tccttcgaca acagcttcaa caacaaggtg
     2941 ctcgtgaagc aggaagaaaa cagcaagaag ggcaaccgga ccccattcca gtacctgagc
     3001 agcagcgaca gcaagatcag ctacgaaacc ttcaagaagc acatcctgaa tctggccaag
     3061 ggcaagggca gaatcagcaa gaccaagaaa gagtatctgc tggaagaacg ggacatcaac
     3121 aggttctccg tgcagaaaga cttcatcaac cggaacctgg tggataccag atacgccacc
     3181 agaggcctga tgaacctgct gcggagctac ttcagagtga acaacctgga cgtgaaagtg
     3241 aagtccatca atggcggctt caccagcttt ctgcggcgga agtggaagtt taagaaagag
     3301 cggaacaagg ggtacaagca ccacgccgag gacgccctga tcattgccaa cgccgatttc
     3361 atcttcaaag agtggaagaa actggacaag gccaaaaaag tgatggaaaa ccagatgttc
     3421 gaggaaaagc aggccgagag catgcccgag atcgaaaccg agcaggagta caaagagatc
     3481 ttcatcaccc cccaccagat caagcacatt aaggacttca aggactacaa gtacagccac
     3541 cgggtggaca agaagcctaa tagagagctg attaacgaca ccctgtactc cacccggaag
     3601 gacgacaagg gcaacaccct gatcgtgaac aatctgaacg gcctgtacga caaggacaat
     3661 gacaagctga aaaagctgat caacaagagc cccgaaaagc tgctgatgta ccaccacgac
     3721 ccccagacct accagaaact gaagctgatt atggaacagt acggcgacga gaagaatccc
     3781 ctgtacaagt actacgagga aaccgggaac tacctgacca agtactccaa aaaggacaac
     3841 ggccccgtga tcaagaagat taagtattac ggcaacaaac tgaacgccca tctggacatc
     3901 accgacgact accccaacag cagaaacaag gtcgtgaagc tgtccctgaa gccctacaga
     3961 ttcgacgtgt acctggacaa tggcgtgtac aagttcgtga ccgtgaagaa tctggatgtg
     4021 atcaaaaaag aaaactacta cgaagtgaat agcaagtgct atgaggaagc taagaagctg
     4081 aagaagatca gcaaccaggc cgagtttatc gcctccttct acaacaacga tctgatcaag
     4141 atcaacggcg agctgtatag agtgatcggc gtgaacaacg acctgctgaa ccggatcgaa
     4201 gtgaacatga tcgacatcac ctaccgcgag tacctggaaa acatgaacga caagaggccc
     4261 cccaggatca ttaagacaat cgcctccaag acccagagca ttaagaagta cagcacagac
     4321 attctgggca acctgtatga agtgaaatct aagaagcacc ctcagatcat caaaaagggc
     4381 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaagga attcggcagt
     4441 ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg
     4501 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     4561 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     4621 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     4681 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     4741 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     4801 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     4861 gcgtggttcc tggccaccgt cggagtctcg cccgaccacc agggcaaggg tctgggcagc
     4921 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     4981 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     5041 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagaa
     5101 ttctaactag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5161 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5221 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     5281 gggtggggca ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagc
     5341 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     5401 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     5461 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     5521 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     5581 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     5641 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5701 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5761 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5821 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5881 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     5941 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6001 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     6061 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     6121 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     6181 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     6241 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     6301 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6361 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6421 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6481 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     6541 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6601 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     6661 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6721 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6781 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6841 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6901 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6961 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7021 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7081 ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta tcattgcagc
     7141 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7201 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7261 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7321 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7381 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7441 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7501 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7561 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     7621 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7681 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7741 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7801 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7861 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7921 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7981 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8041 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
