LOCUS       sequence-354859-        9130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mCherry with early endosome-targeting sequence in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Kashiwagi S, Fujioka Y, Satoh AO, Yoshida A, Fujioka M, Nepal P, 
            Tsuzuki A, Aoki O, Paudel S, Sasajima H, Ohba Y
  TITLE     Folding Latency of Fluorescent Proteins Affects the Mitochondrial 
            Localization of Fusion Proteins.
  JOURNAL   Cell Struct Funct. 2019 Dec 26;44(2):183-194. doi: 
            10.1247/csf.19028. Epub 2019 Nov 15.
  PUBMED    31735740
REFERENCE   2  (bases 1 to 9130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1247/csf.19028";
            journalName: "Cell Struct Funct"; date: "2019-12-26- 26"; volume: 
            "44"; issue: "2"; pages: "183-194"
COMMENT     Sequence Label: pFX-mCherry-EEA1
FEATURES             Location/Qualifiers
     source          1..9130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             627..1334
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(772..790)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1040..1059
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1383..5618
                     /codon_start=1
                     /product="human early endosome antigen 1"
                     /label=EEA1
                     /translation="MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQ
                     CMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKW
                     YSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQ
                     KAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIED
                     VAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQ
                     EVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLH
                     QKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQ
                     QQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADL
                     QLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQ
                     IGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQ
                     VTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKE
                     KVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVT
                     AKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDL
                     QQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKQIL
                     KQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKN
                     SKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLK
                     LELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQK
                     TELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQD
                     LKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQD
                     IQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQ
                     LLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEK
                     EVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEAT
                     VQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQ
                     ALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVC
                     DACFNDLQG"
     polyA_signal    5918..6039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5955..5974)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6009..6028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6046..6501)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6183..6204)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6395..6414
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6528..6632
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6634..6991
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6634..6825
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6657..6677)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6842..6977
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7026..7820
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7026..7820
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7080..7099)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7690..7709
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8008..8027)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8052..8099
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8428..9016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8917..8936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggttctag aagtactgaa ttcaccatgg tgagcaaggg cgaggaggat aacatggcca
      661 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      721 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      781 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
      841 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
      901 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
      961 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     1021 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     1081 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     1141 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     1201 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     1261 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     1321 acgagctgta caagggaggc tccggaggaa gatctggagg atccggagga ctcgagccca
     1381 ccatgttaag gaggatttta cagaggactc ctgggagagt tggctctcaa ggttctgatt
     1441 tagattcatc agcaactcct ataaacacag tggacgtcaa taatgaaagc tcttcagaag
     1501 gtttcatatg tccccagtgt atgaaatctc ttggatctgc tgatgaactt ttcaaacatt
     1561 atgaagctgt tcatgatgct ggtaatgact caggtcatgg aggagagtct aatcttgctt
     1621 tgaagcgaga tgatgtaaca ctgctcagac aagaggtcca agacctacag gcttcactta
     1681 aggaagaaaa atggtactcg gaagaattaa agaaggaatt agaaaaatat caagggctgc
     1741 agcagcaaga ggccaaacct gatgggttgg tgactgattc atcagcagaa ctacagtctt
     1801 tggaacagca attagaagaa gcccaaacag aaaattttaa tattaagcaa atgaaagact
     1861 tatttgaaca gaaagcagcc caacttgcta ctgaaattgc agatataaag tcaaagtatg
     1921 atgaagaaag gagtcttcga gaagctgctg aacaaaaagt gacacgtctg acagaagaat
     1981 taaacaaaga ggcaactgta attcaagatc tgaagacgga actgcttcag agacctggta
     2041 tagaagatgt tgccgtgcta aagaaagaac tggtccaagt tcaaacacta atggataaca
     2101 tgaccttgga acgtgagcga gaatctgaaa aactcaaaga tgaatgcaaa aaattgcagt
     2161 cacaatatgc tagctcagag gccacaataa gccagctaag gagtgaactt gccaaaggcc
     2221 cccaggaagt tgctgtatat gtacaggaac tacaaaaact gaaaagttca gttaatgaat
     2281 taacacaaaa aaatcagacc ttgacagaaa acttgctgaa aaaagaacaa gactatacta
     2341 agttagagga gaaacataat gaagaatctg tgagtaaaaa gaatattcag gcaacccttc
     2401 atcaaaaaga cctagattgt caacagcttc agtcaagatt gtctgcatct gaaacctcac
     2461 tgcatagaat acatgtagaa ctaagtgaaa aaggagaagc tactcaaaag ctcaaagaag
     2521 aattatctga ggtagagacc aagtaccagc atctaaaggc ggagtttaag cagctacaac
     2581 aacagagaga agaaaaggag cagcatgggt tacaactcca aagtgaaatt aatcaattac
     2641 atagcaaact tctagagaca gagcgccaac taggggaagc tcatggtagg ctgaaggaac
     2701 agagacagct ttcaagtgaa aagttgatgg ataaagaaca acaagtggct gatttacaac
     2761 tcaaactttc tcggttagaa gagcagttga aggaaaaagt tacaaattct acagaattgc
     2821 agcatcaatt agataaaaca aagcaacagc atcaagaaca acaggctctt cagcaaagca
     2881 ccacggcaaa acttcgagaa gctcagaatg atttggaaca agttctacgt caaattggcg
     2941 ataaggacca aaagatccag aaccttgaag ctttattaca gaagagtaaa gaaaatattt
     3001 cattactaga aaaagaaaga gaagatcttt atgcaaaaat tcaggctggt gaaggagaga
     3061 ctgctgttct taaccagtta caagaaaaaa accatacact acaggagcaa gtaactcaac
     3121 taacagagaa gctgaagaat cagtcagaaa gtcataaaca agcccaggag aatttgcatg
     3181 accaggtaca agagcagaag gcacatctta gagctgcaca agaccgtgtc ctttccctag
     3241 aaactagtgt caatgaatta aatagtcaat taaatgaaag caaggagaag gtctcccagc
     3301 ttgacataca gattaaagcc aaaaccgaac tattactatc agcagaagca gcaaaaactg
     3361 ctcaaagagc tgatcttcag aatcatttgg acacagctca aaatgcatta caagataaac
     3421 agcaggagtt aaataagatt actactcagt tggatcaggt cactgcaaag ttacaagaca
     3481 agcaagaaca ttgcagtcag ctggaaagtc atcttaaaga atataaagag aaatacctct
     3541 ctttagaaca gaaaaccgaa gagctagaag gtcaaattaa gaaactagaa gctgatagtc
     3601 ttgaagttaa agcaagcaag gagcaggctt tgcaagatct acaacagcaa agacagctga
     3661 acacagattt agagctcaga gccacagaat tgagtaaaca acttgaaatg gagaaggaaa
     3721 tagtatccag tacaagattg gatctacaga aaaaatctga agcccttgaa agtatcaagc
     3781 aaaagcttac caagcaagag gaagaaaaac aaatcctgaa acaagatttt gaaactttaa
     3841 gtcaagaaac aaagattcag catgaggaat tgaataacag aattcaaaca acagtaacag
     3901 aactacaaaa agtgaaaatg gagaaagaag ctttaatgac agagctttct acagtaaagg
     3961 acaaactatc aaaagtttct gattctttga aaaactctaa aagtgaattt gaaaaggaga
     4021 atcagaaagg aaaagccgct atattagact tggaaaaaac ttgcaaagaa ttaaagcatc
     4081 aacttcaagt gcagatggaa aacacactta aggaacagaa ggaactgaaa aagtcacttg
     4141 aaaaagagaa ggaggcttct catcagttga aattggaact caattcaatg caggaacaac
     4201 ttatacaggc ccagaatact ttaaaacaaa atgaaaaaga agagcaacaa cttcagggga
     4261 acataaatga gctaaagcaa tcaagtgaac agaagaaaaa acaaattgaa gcactccaag
     4321 gagagcttaa aattgctgtt ttacagaaga cagagcttga gaataaacta cagcagcagt
     4381 taacacaggc agcccaggaa cttgcagcag agaaagagaa aatatcagta ttacaaaaca
     4441 actatgaaaa aagtcaggaa actttcaaac agcttcaatc tgatttctat gggagggaat
     4501 ctgaacttct agccaccagg caagatctta agtctgtaga agagaagctt tctctagcac
     4561 aggaggactt gatttcaaac agaaatcaaa ttggaaatca aaataaattg attcaagaac
     4621 tgaagactgc caaggctaca ttggagcagg attcagcaaa gaaagaacag caattgcagg
     4681 agcgatgtaa agcactacaa gacattcaga aagaaaagtc actgaaagaa aaagaactgg
     4741 taaatgagaa gtctaaattg gcagagatag aagaaattaa atgtagacaa gaaaaagaaa
     4801 tcactaaact aaacgaagaa ctcaagtccc acaaactaga aagcataaag gagataacaa
     4861 atcttaaaga tgctaaacag cttctaattc agcagaaatt agaacttcaa ggaaaagcgg
     4921 actccctgaa ggcagctgtt gaacaggaga agagaaatca gcagatacta aaagaccagg
     4981 tgaaaaagga agaagaggag ctgaagaaag aatttattga gaaagaagct aagttgcatt
     5041 ccgaaataaa agaaaaggaa gtaggaatga agaagcatga agaaaatgag gctaaactta
     5101 ccatgcagat tacagcatta aatgaaaact taggcactgt gaagaaggag tggcaatcta
     5161 gtcaacggag agttagtgag cttgagaaac aaacggatga cttacggggt gaaattgcag
     5221 tattagaagc aacggttcag aataatcaag atgaaaggag agcactactg gaaagatgtc
     5281 ttaaaggaga aggtgaaata gaaaagcttc aaaccaaagt attagaattg caaagaaagc
     5341 tggataatac aactgcagca gtgcaggagc tgggcagaga aaaccaatca cttcagatca
     5401 aacatacaca agcgttgaat agaaagtggg ccgaagacaa tgaagtacaa aactgtatgg
     5461 cctgtgggaa aggcttttca gtaacagtga gacggcatca ctgccgacag tgtggaaata
     5521 tcttctgtgc tgaatgttca gccaaaaatg ccttaactcc ttcctccaag aagcctgttc
     5581 gtgtctgtga tgcatgtttc aatgacttgc aaggataatg ggttatcaca acttcagagt
     5641 aatattacac taacattaga tttttaataa atgtacttaa tagaggtctt ggactactat
     5701 ttggtttgga cactggttgc aatactagac caaataggaa ttagtatatt ttggaatgtt
     5761 atggatatcg cggccgctga ctgactgaag atccaccgga tctagataac tgatcataat
     5821 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     5881 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     5941 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6001 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
     6061 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     6121 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     6181 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     6241 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     6301 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     6361 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     6421 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     6481 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6541 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6601 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     6661 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6721 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     6781 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     6841 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6901 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6961 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     7021 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     7081 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     7141 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     7201 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     7261 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     7321 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     7381 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     7441 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     7501 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     7561 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     7621 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     7681 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     7741 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     7801 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     7861 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7921 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7981 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     8041 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     8101 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     8161 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     8221 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     8281 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8341 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8401 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8461 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8521 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8581 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8641 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8701 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8761 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8821 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8881 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8941 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9001 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9061 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9121 cgccatgcat
//
