LOCUS       sequence-354863-        9124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express EGFP with early endosome-targeting sequence in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Kashiwagi S, Fujioka Y, Satoh AO, Yoshida A, Fujioka M, Nepal P, 
            Tsuzuki A, Aoki O, Paudel S, Sasajima H, Ohba Y
  TITLE     Folding Latency of Fluorescent Proteins Affects the Mitochondrial 
            Localization of Fusion Proteins.
  JOURNAL   Cell Struct Funct. 2019 Dec 26;44(2):183-194. doi: 
            10.1247/csf.19028. Epub 2019 Nov 15.
  PUBMED    31735740
REFERENCE   2  (bases 1 to 9124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1247/csf.19028";
            journalName: "Cell Struct Funct"; date: "2019-12-26- 26"; volume: 
            "44"; issue: "2"; pages: "183-194"
COMMENT     Sequence Label: pFX-EGFP-EEA1
FEATURES             Location/Qualifiers
     source          1..9124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(627..629,630..632,633..1343)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(627..629,630..632,633..1343)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(672..693)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(933..952)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1280..1301
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1377..5612
                     /codon_start=1
                     /product="human early endosome antigen 1"
                     /label=EEA1
                     /translation="MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQ
                     CMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKW
                     YSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQ
                     KAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIED
                     VAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQ
                     EVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLH
                     QKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQ
                     QQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADL
                     QLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQ
                     IGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQ
                     VTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKE
                     KVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVT
                     AKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDL
                     QQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKQIL
                     KQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKN
                     SKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLK
                     LELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQK
                     TELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQD
                     LKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQD
                     IQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQ
                     LLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEK
                     EVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEAT
                     VQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQ
                     ALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVC
                     DACFNDLQG"
     polyA_signal    5912..6033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5949..5968)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6003..6022
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6040..6495)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6177..6198)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6389..6408
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6522..6626
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6628..6985
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6628..6819
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6651..6671)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6836..6971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7020..7814
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7020..7814
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7074..7093)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7684..7703
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8002..8021)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8046..8093
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8422..9010
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8911..8930
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggttctag aagtactgaa ttcaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      661 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      721 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      781 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      841 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      901 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      961 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1021 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1081 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1141 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1201 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     1261 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1321 tcggcatgga cgagctgtac aagagatctg gaggatccgg aggactcgag cccaccatgt
     1381 taaggaggat tttacagagg actcctggga gagttggctc tcaaggttct gatttagatt
     1441 catcagcaac tcctataaac acagtggacg tcaataatga aagctcttca gaaggtttca
     1501 tatgtcccca gtgtatgaaa tctcttggat ctgctgatga acttttcaaa cattatgaag
     1561 ctgttcatga tgctggtaat gactcaggtc atggaggaga gtctaatctt gctttgaagc
     1621 gagatgatgt aacactgctc agacaagagg tccaagacct acaggcttca cttaaggaag
     1681 aaaaatggta ctcggaagaa ttaaagaagg aattagaaaa atatcaaggg ctgcagcagc
     1741 aagaggccaa acctgatggg ttggtgactg attcatcagc agaactacag tctttggaac
     1801 agcaattaga agaagcccaa acagaaaatt ttaatattaa gcaaatgaaa gacttatttg
     1861 aacagaaagc agcccaactt gctactgaaa ttgcagatat aaagtcaaag tatgatgaag
     1921 aaaggagtct tcgagaagct gctgaacaaa aagtgacacg tctgacagaa gaattaaaca
     1981 aagaggcaac tgtaattcaa gatctgaaga cggaactgct tcagagacct ggtatagaag
     2041 atgttgccgt gctaaagaaa gaactggtcc aagttcaaac actaatggat aacatgacct
     2101 tggaacgtga gcgagaatct gaaaaactca aagatgaatg caaaaaattg cagtcacaat
     2161 atgctagctc agaggccaca ataagccagc taaggagtga acttgccaaa ggcccccagg
     2221 aagttgctgt atatgtacag gaactacaaa aactgaaaag ttcagttaat gaattaacac
     2281 aaaaaaatca gaccttgaca gaaaacttgc tgaaaaaaga acaagactat actaagttag
     2341 aggagaaaca taatgaagaa tctgtgagta aaaagaatat tcaggcaacc cttcatcaaa
     2401 aagacctaga ttgtcaacag cttcagtcaa gattgtctgc atctgaaacc tcactgcata
     2461 gaatacatgt agaactaagt gaaaaaggag aagctactca aaagctcaaa gaagaattat
     2521 ctgaggtaga gaccaagtac cagcatctaa aggcggagtt taagcagcta caacaacaga
     2581 gagaagaaaa ggagcagcat gggttacaac tccaaagtga aattaatcaa ttacatagca
     2641 aacttctaga gacagagcgc caactagggg aagctcatgg taggctgaag gaacagagac
     2701 agctttcaag tgaaaagttg atggataaag aacaacaagt ggctgattta caactcaaac
     2761 tttctcggtt agaagagcag ttgaaggaaa aagttacaaa ttctacagaa ttgcagcatc
     2821 aattagataa aacaaagcaa cagcatcaag aacaacaggc tcttcagcaa agcaccacgg
     2881 caaaacttcg agaagctcag aatgatttgg aacaagttct acgtcaaatt ggcgataagg
     2941 accaaaagat ccagaacctt gaagctttat tacagaagag taaagaaaat atttcattac
     3001 tagaaaaaga aagagaagat ctttatgcaa aaattcaggc tggtgaagga gagactgctg
     3061 ttcttaacca gttacaagaa aaaaaccata cactacagga gcaagtaact caactaacag
     3121 agaagctgaa gaatcagtca gaaagtcata aacaagccca ggagaatttg catgaccagg
     3181 tacaagagca gaaggcacat cttagagctg cacaagaccg tgtcctttcc ctagaaacta
     3241 gtgtcaatga attaaatagt caattaaatg aaagcaagga gaaggtctcc cagcttgaca
     3301 tacagattaa agccaaaacc gaactattac tatcagcaga agcagcaaaa actgctcaaa
     3361 gagctgatct tcagaatcat ttggacacag ctcaaaatgc attacaagat aaacagcagg
     3421 agttaaataa gattactact cagttggatc aggtcactgc aaagttacaa gacaagcaag
     3481 aacattgcag tcagctggaa agtcatctta aagaatataa agagaaatac ctctctttag
     3541 aacagaaaac cgaagagcta gaaggtcaaa ttaagaaact agaagctgat agtcttgaag
     3601 ttaaagcaag caaggagcag gctttgcaag atctacaaca gcaaagacag ctgaacacag
     3661 atttagagct cagagccaca gaattgagta aacaacttga aatggagaag gaaatagtat
     3721 ccagtacaag attggatcta cagaaaaaat ctgaagccct tgaaagtatc aagcaaaagc
     3781 ttaccaagca agaggaagaa aaacaaatcc tgaaacaaga ttttgaaact ttaagtcaag
     3841 aaacaaagat tcagcatgag gaattgaata acagaattca aacaacagta acagaactac
     3901 aaaaagtgaa aatggagaaa gaagctttaa tgacagagct ttctacagta aaggacaaac
     3961 tatcaaaagt ttctgattct ttgaaaaact ctaaaagtga atttgaaaag gagaatcaga
     4021 aaggaaaagc cgctatatta gacttggaaa aaacttgcaa agaattaaag catcaacttc
     4081 aagtgcagat ggaaaacaca cttaaggaac agaaggaact gaaaaagtca cttgaaaaag
     4141 agaaggaggc ttctcatcag ttgaaattgg aactcaattc aatgcaggaa caacttatac
     4201 aggcccagaa tactttaaaa caaaatgaaa aagaagagca acaacttcag gggaacataa
     4261 atgagctaaa gcaatcaagt gaacagaaga aaaaacaaat tgaagcactc caaggagagc
     4321 ttaaaattgc tgttttacag aagacagagc ttgagaataa actacagcag cagttaacac
     4381 aggcagccca ggaacttgca gcagagaaag agaaaatatc agtattacaa aacaactatg
     4441 aaaaaagtca ggaaactttc aaacagcttc aatctgattt ctatgggagg gaatctgaac
     4501 ttctagccac caggcaagat cttaagtctg tagaagagaa gctttctcta gcacaggagg
     4561 acttgatttc aaacagaaat caaattggaa atcaaaataa attgattcaa gaactgaaga
     4621 ctgccaaggc tacattggag caggattcag caaagaaaga acagcaattg caggagcgat
     4681 gtaaagcact acaagacatt cagaaagaaa agtcactgaa agaaaaagaa ctggtaaatg
     4741 agaagtctaa attggcagag atagaagaaa ttaaatgtag acaagaaaaa gaaatcacta
     4801 aactaaacga agaactcaag tcccacaaac tagaaagcat aaaggagata acaaatctta
     4861 aagatgctaa acagcttcta attcagcaga aattagaact tcaaggaaaa gcggactccc
     4921 tgaaggcagc tgttgaacag gagaagagaa atcagcagat actaaaagac caggtgaaaa
     4981 aggaagaaga ggagctgaag aaagaattta ttgagaaaga agctaagttg cattccgaaa
     5041 taaaagaaaa ggaagtagga atgaagaagc atgaagaaaa tgaggctaaa cttaccatgc
     5101 agattacagc attaaatgaa aacttaggca ctgtgaagaa ggagtggcaa tctagtcaac
     5161 ggagagttag tgagcttgag aaacaaacgg atgacttacg gggtgaaatt gcagtattag
     5221 aagcaacggt tcagaataat caagatgaaa ggagagcact actggaaaga tgtcttaaag
     5281 gagaaggtga aatagaaaag cttcaaacca aagtattaga attgcaaaga aagctggata
     5341 atacaactgc agcagtgcag gagctgggca gagaaaacca atcacttcag atcaaacata
     5401 cacaagcgtt gaatagaaag tgggccgaag acaatgaagt acaaaactgt atggcctgtg
     5461 ggaaaggctt ttcagtaaca gtgagacggc atcactgccg acagtgtgga aatatcttct
     5521 gtgctgaatg ttcagccaaa aatgccttaa ctccttcctc caagaagcct gttcgtgtct
     5581 gtgatgcatg tttcaatgac ttgcaaggat aatgggttat cacaacttca gagtaatatt
     5641 acactaacat tagattttta ataaatgtac ttaatagagg tcttggacta ctatttggtt
     5701 tggacactgg ttgcaatact agaccaaata ggaattagta tattttggaa tgttatggat
     5761 atcgcggccg ctgactgact gaagatccac cggatctaga taactgatca taatcagcca
     5821 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     5881 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     5941 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6001 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
     6061 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     6121 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     6181 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     6241 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     6301 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     6361 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     6421 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6481 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6541 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6601 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     6661 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6721 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6781 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6841 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6901 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6961 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     7021 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     7081 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     7141 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     7201 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     7261 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     7321 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7381 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7441 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7501 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     7561 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     7621 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     7681 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     7741 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     7801 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     7861 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7921 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7981 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     8041 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     8101 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     8161 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     8221 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     8281 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8341 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8401 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8461 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8521 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8581 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8641 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8701 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8761 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8821 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8881 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8941 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9001 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9061 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
     9121 gcat
//
