LOCUS       sequence-354889-        3903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FnCas12a in Bacteroides and used for genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Zheng L, Tan Y, Hu Y, Shen J, Qu Z, Chen X, Ho CL, Leung EL, Zhao W,
            Dai L
  TITLE     CRISPR/Cas-Based Genome Editing for Human Gut Commensal Bacteroides 
            Species.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):464-472. doi: 
            10.1021/acssynbio.1c00543. Epub 2022 Jan 6.
  PUBMED    34990118
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00543"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "464-472"
COMMENT     Sequence Label: pB025
FEATURES             Location/Qualifiers
     source          1..3903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagcatct atcaagaatt tgtgaataaa tatagtctta gtaaaacatt gagattcgaa
       61 ctgatcccgc aaggaaaaac tctggaaaat attaaagcac gtggattgat tttggatgat
      121 gaaaaacgtg ctaaagatta taaaaaagcc aaacagataa ttgataaata tcatcaattc
      181 ttcattgaag aaatccttag tagtgtatgc attagcgaag atctgcttca gaattatagt
      241 gatgtatatt ttaaactgaa aaaatctgat gatgataatt tacagaaaga ctttaaatca
      301 gctaaagata caataaagaa acaaattagt gaatatatta aagatagtga aaagtttaaa
      361 aatttgttta accagaatct gatcgatgct aaaaaaggac aggaatccga tctgattctg
      421 tggttgaaac agagtaaaga taatggtatt gaattgttta aagccaattc tgatatcact
      481 gacattgatg aagcactgga aattatcaaa tcctttaaag gttggactac ctattttaaa
      541 ggttttcacg aaaatcgtaa aaatgtgtat tctagtaatg atataccgac gtcgattatc
      601 tatcgtatcg tagatgataa tctgccgaaa tttctggaaa ataaagctaa atacgaatct
      661 ctgaaagata aagcacccga agccatcaat tatgaacaga ttaagaagga tctggctgaa
      721 gaactgacat ttgacataga ttataaaacg tctgaagtaa atcagcgcgt atttagtctg
      781 gatgaagtgt tcgaaatcgc aaattttaac aattacttaa atcagtcggg tataaccaag
      841 tttaatacca taatcggagg aaaatttgta aatggagaaa atacgaaacg caaagggatc
      901 aatgaatata tcaatttgta ttctcagcag attaacgata aaacattgaa aaaatataaa
      961 atgagtgtac tgttcaaaca aatcctgtct gataccgaat ctaaaagttt cgtaatcgat
     1021 aaattagaag atgattctga tgtggtgact acaatgcaga gcttctatga acagatcgct
     1081 gcattcaaaa ctgtagaaga aaagagtatt aaggaaacac tgtctttgct gtttgatgat
     1141 ctgaaagctc agaaacttga cttaagcaaa atctacttca aaaacgataa aagtctgact
     1201 gatttaagcc agcaagtttt cgacgactat agtgtaattg gcaccgccgt attggaatat
     1261 atcactcagc aaattgcccc taaaaatctg gataacccgt cgaagaaaga acaggaactg
     1321 attgccaaga aaaccgaaaa ggctaaatat ctgagcttgg aaacaatcaa actcgcactg
     1381 gaagaattca ataagcatcg ggatattgat aaacaatgtc gttttgaaga aattctggcc
     1441 aattttgctg caattcctat gatattcgat gaaattgcac aaaataaaga taacttagcc
     1501 cagatttcta ttaaatatca gaaccagggt aaaaaagatc ttttgcaggc gagtgccgaa
     1561 gatgatgtaa aggctattaa agatctgctg gatcaaacta ataatttgtt acataaactg
     1621 aagattttcc atatctcgca gtctgaagat aaagctaata ttttggataa agacgaacat
     1681 ttctatctgg tatttgaaga atgttatttc gaattagcaa acattgttcc gctgtataac
     1741 aaaattcgta attatattac tcaaaaaccg tattcagatg aaaaatttaa actgaatttt
     1801 gaaaatagta cattagctaa cggatgggat aagaataaag aacctgataa tacagcgatt
     1861 ttattcatca aagacgataa atactatttg ggagttatga ataagaaaaa taataaaatc
     1921 tttgatgata aagctattaa agaaaataaa ggagaaggtt ataagaaaat agtttataaa
     1981 ttgctgccag gagctaataa gatgctgcct aaagtatttt tcagtgctaa atctattaaa
     2041 ttctataacc cgtcagagga catcttaaga attcggaatc atagcaccca tacaaagaat
     2101 ggatctcccc aaaaaggata tgaaaagttc gaatttaata ttgaagattg cagaaaattt
     2161 attgatttct ataaacaatc tattagtaaa catcccgaat ggaaagattt cggctttcgt
     2221 ttctctgata cacaaagata taatagtatt gatgaatttt atagagaagt agaaaatcag
     2281 ggctataagc tgacgttcga aaatatttca gaatcctata tagatagtgt agttaatcag
     2341 ggtaaattgt atttatttca gatttataat aaagactttt ctgcctattc taaaggacgt
     2401 cctaatctgc atacactcta ttggaaagct ttattcgatg aacgtaacct ccaagatgta
     2461 gtatataagc tcaatggaga agcagaattg ttctatcgca agcaaagtat tcctaaaaag
     2521 atcactcatc ctgctaaaga agccattgct aataagaata aagataaccc taagaaagaa
     2581 tcagtatttg aatatgattt gataaaagat aaacgtttca ctgaagataa gtttttcttc
     2641 cattgtccga taacaattaa ctttaagtcc agtggagcaa ataaattcaa tgatgaaatc
     2701 aatctgcttc ttaaagaaaa agcaaatgat gtacatattt tgtctattga tagaggtgaa
     2761 cgtcaccttg catattatac tttagtggat ggaaagggaa atatcatcaa gcaggatacc
     2821 tttaatatta tcggaaatga ccgtatgaaa acgaactatc atgataaatt ggctgctatt
     2881 gaaaaggatc gtgatagcgc tcgtaaagac tggaagaaaa ttaataatat caaagaaatg
     2941 aaagagggat atctgtcgca ggtagtacac gaaatcgcta aactggtaat tgaatataat
     3001 gccatcgtag tatttgagga tctgaatttc ggtttcaagc gtggacgttt taaagtagaa
     3061 aagcaagtat atcagaaatt agaaaagatg ttaattgaaa aattaaatta tctggttttc
     3121 aaagataatg aattcgataa aaccggaggc gtcttgagag cctatcagtt aactgcccct
     3181 tttgaaactt ttaaaaagat gggtaaacag acaggaatta tttattatgt gccggccgga
     3241 tttacatcta aaatctgccc ggtgactggt ttcgtgaacc aactgtatcc gaaatatgaa
     3301 agtgttagca aatcccagga attctttagc aaatttgata aaatatgtta taatttggat
     3361 aaaggatatt ttgaattcag ttttgactat aaaaattttg gagataaagc cgcaaagggt
     3421 aagtggacta ttgctagttt cggatcgcgc ttgattaatt ttcgtaattc agataaaaac
     3481 cataattggg atactcgtga agtgtatcct accaaagaac tggaaaaatt gttaaaggac
     3541 tatagtattg aatatggaca tggtgaatgt ataaaagcag caatatgcgg agaaagtgat
     3601 aagaaattct ttgcaaaatt gactagcgta ctgaatacta tattgcaaat gcgtaatagt
     3661 aaaacaggaa cagaactgga ttatctgatc agccctgtag cagacgtaaa cggcaatttc
     3721 tttgatagtc gccaagctcc taaaaatatg ccgcaagacg ctgatgcaaa tggagcatat
     3781 cacattggtc ttaagggact gatgctgctg ggtcgcatta aaaataatca ggaaggtaaa
     3841 aaactgaact tggtaatcaa aaatgaagaa tattttgaat tcgtacagaa tcgtaataat
     3901 taa
//
