LOCUS       sequence-354908-        8944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-2::D3cpv unc-54 3' UTR C.elegans neural expression of D3cpv.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 8944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH1863
FEATURES             Location/Qualifiers
     source          1..8944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..25
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9..25
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(3661..3663,3664..3666,3667..4179)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             4885..5619
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     complement(6538..6560)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6660..6679
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6757..6775)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6843..6947
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6948..7016,7017..7808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7166..7185)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7979..8567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8468..8487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8721..8738
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8855..8876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8891..8896,8897..8914,8915..8921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(8929..8944,1)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8934..8944,1..12)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atttcacaca ggaaacagct atgaccatga ttacgccaag ctgtaagttt aaacatgatc
       61 ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga aatcactcac
      121 aacgatggat acgctaacaa cttggaaatg aaataagctt gggctgcagc atgcggccgc
      181 attcttggaa tttcgcgttt tccattttcc gttgtttttt cattttataa aagtttcagc
      241 acctacattt ttcaatattt cttcaatgga gaataaatca gttgaatcag ttgaaaaaca
      301 aagtcagagc tgtggaagtc gaaatttaag agcttttttt ttaatttttt atagttgttg
      361 taaagcttat tagtataccg cacctactgt taatatgaac catcaaatgt tactcttcct
      421 ttttcattaa aaaaaacttt cagtttgccc cgccggtgcg attccttata agatgccaca
      481 agttttgtta tgcgacccga caattgttaa tatccgtcca tacgactata aggtttggtt
      541 ttattcgcat tttattgttt tgtttttgga tcaactctat caaaaaaaca atattcgttc
      601 atgatactaa tgactaatga gttttcagtg cgttgaagca gcaaacggac aatatcttcc
      661 tgccgatggc cgatctcttt gctgcaagac caccactctg tactcgtttg cctcggtttt
      721 tggagaggct aaaataagcc ctcggctagt gccaaatccc cctctttcag cgatcgaata
      781 tgtgcgtcgg tattttttgt tcctcttatt ttttgtctta gacatcaacg ccgccgccgt
      841 aaaacagaag acatgtttcc gtacttccag gtcactttga acgttcacac atccgccaaa
      901 gagcactcac cggaaattag aactggagat cattttgtgc tgacaccata caaactctac
      961 gagccggcat atttgaaaaa aatcaaactg ttcacagact ttggtcatgg ctcatttgtg
     1021 catgttatac tttttggtaa atctattgaa catgttcgga gtgttcatgc gagcgtaact
     1081 aagttttgag aaaaaaagaa tgagagaata actagggctt ttgtcttttt ttgtgaatat
     1141 ctggttatgt ttgaagatcc aatgtcaaca actgaaacaa tgcaacttta ttatgaccga
     1201 ccaacatcca atgggaaaac tatcaattta gatgagccaa ttccagacgg aggtttcaat
     1261 aattaaaaaa aaattaagaa taatcaaata acatgcgctt ttcaggattc atttctaaga
     1321 aaatattcaa tgcggctcca attacacaaa tagaaaaccc atcacgtccg ggtccaataa
     1381 aagaatgtga gaactttctt cttgaaaaat gaacatattg aattgcacca aacgcaaaca
     1441 agaaactcta aatttcagat cgaaaattgt ggattgtgct cattttcaag accacaagcc
     1501 cgtttggtcg agtttatatt ctttccacca aggatttgca cagcaagtat agatcagtca
     1561 ccgatttttt gaaatccgac acaggaaaca tgctaggaac tcctgtggct ggaacttatt
     1621 tttatgtgag ttttgttttg aaccctcaac tataaaattg tcggcccgtt ttgttttcag
     1681 ctcaccaccg actgattttc atttcaaaat taatgcagat ttctacgtgt tctttttcaa
     1741 ataaatttat atattttttg ttctctcaca aaataaataa tttcgagagt taagtgatca
     1801 ttctcagaca tattctataa ttgtccgtca ggaaacagat agaggaacct ttgataaata
     1861 aattataaaa ggccgacagg gaaatgcttc tccctcgact ctgttagaag cgaagaggca
     1921 atttttaaaa atttcccatt tttatgcgtt tttctgattt ccaaagtcac gcatcattta
     1981 ccaaccgcaa cgattcataa ggacgtaatg ttgattttct ggacgatttt tatatagttt
     2041 tgatgtgaaa ttcgctttat ttgtctgaaa gtggaacaga tttagtgtgg atttaaccga
     2101 aaaatgcaga tcttttaaaa atttaaacca gtgttttgtt gccttatttg cgaaaaaaaa
     2161 ttgtgatatt tcgagtattg ctgcaatttg attagtaaaa tgttaggata taagtattta
     2221 aaaagtataa ttggttgaat tttctttttc atcaccgact tccaaaaata tagagatact
     2281 gagttttcac cattttttga atgtaaataa aaacattttg aacacttgtg ctgggattca
     2341 atccaaatac attcactgtc atttaatatt attatatatc aaaataaaac gcataacttt
     2401 ttctgttttt ttttaaattt tttcaaaatg taggtttggt aagttaaggt aaggttaggc
     2461 aatgtaggta aacacgagat gatactccac gttgaaaatt gttttcataa atacagttta
     2521 gattgaactg aaggatttta tcgtctaata gaatataatt ttttagttca agatttagga
     2581 tttgttttca ctttttttta gattcaagac atatatctcg caaaatgagt tttgtctttg
     2641 agccatcttg tcatcatagc acattgaatt ttgttgttcg caagacggaa tatatgttcc
     2701 cagctgcaaa ttttgtgtcg gatatttctt ctttagcgaa aggcgttaaa aaccaaaaat
     2761 agtcgaagcg tttgatatgt gtcccgttgg tttttcgtgt ccatctattc tgtttcgagt
     2821 acacatgttg cgacgcggca ctgatgaaaa agtgaaagag ctagtgtcac acacaaacac
     2881 taagtatgct catgtgagtg tgtagatatg tgtagatgta tgtcctgaag gaataaaaag
     2941 actttttgaa gctacatttt taaacaaaaa aagacagaaa catgaagcac aactctctat
     3001 tctgtcgaac ggcgtgtgca actcacgcac acccaaaaat atgaaatata acattcgcac
     3061 caacaaagcg cgaatatcaa tgaatctcga gggaacgatg gctgcctgcc ggcgcgtgtt
     3121 gctcttctgt cattgggata actggcgggt ctgttgggtc cgtcctttag accatttgac
     3181 tacactgccc tgcatatgtg tgaaacgtcg tcgttgctgt tgggagctgt ctgtgtgtat
     3241 gtgtgtgtgt gtgagtatgg agcagtaaaa agctgccgat acgagaagcg tcgccccgga
     3301 cggacggcga tggcgcatcg ccaaatgtgt cattttagtt gtcttcacga accagagggc
     3361 ccacgacgga cgggcggcca cgcccacctc aaccagaata tccttccttc tcccacccac
     3421 ccttgcggac gcgcggatgc gctgaacaaa aaggcgacga agaagaagaa gaagaagctg
     3481 aacgaagaaa aaagcatcaa aagaagtgaa gcggctctac caccccgacc accttcctcc
     3541 acaggaagga cgccgttttc atcccgggat tggccaaagg acccaaaggt atgtttcgaa
     3601 tgatactaac ataacataga acattttcag gaggaccctt ggctagcgtc gacggatccc
     3661 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     3721 ggcgacgtaa acggccacag gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     3781 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     3841 ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     3901 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg taccatcttc
     3961 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4021 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4081 aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac
     4141 ggcatcaagg cccacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     4201 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     4261 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     4321 ctgctggagt tcgtgaccgc cgcccgcatg catgaccaac tgacagaaga gcagattgca
     4381 gagttcaaag aagccttctc attattggac aaggatgggg acggcaccat caccacaaag
     4441 gaacttggca ccgctctgag gtcgcttgga caaaacccaa cggaagcaga attgcaggat
     4501 atgatcaatg aagtcgatgc tgatggcaat ggaacgattt actttcctga atttcttact
     4561 atgatggcta gaaaaatgaa ggacacagac agcgaagagg aaatccgaga agcattccgt
     4621 gtttttgaca aggatgggaa cggctacatc agcgctgctg aattacgtca cgtcatgaca
     4681 aacctcgggg agaagttaac agatgaagaa gttgatgaaa tgataaggga agcagatatc
     4741 gatggtgatg gccaagtaaa ctatgaagag tttgtacaaa tgatgacagc aaaggggggg
     4801 aagaggcgct ggcagaaaac aggccatgct gtccgagcat tcggaagact gaagaagatc
     4861 tccagctccg gggcactgga gctcatggac ggcggcgtgc agctcgccga ccactaccag
     4921 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     4981 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     5041 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggtgg cagcggtggc
     5101 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     5161 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     5221 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
     5281 ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     5341 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     5401 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     5461 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     5521 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
     5581 ggcatcaagg ccaacttcaa gatccgccac aacatcgagt aagaattcca actgagcgcc
     5641 ggtcgctacc attaccaact tgtctggtgt caaaaataat aggggccgct gtcatcagag
     5701 taagtttaaa ctgagttcta ctaactaacg agtaatattt aaattttcag catctcgcgc
     5761 ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct ctttctccct
     5821 gtgctcccac cccctatttt tgttattatc aaaaaacttc tcttaatttc tttgtttttt
     5881 agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc
     5941 gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa
     6001 aatctacaca atgttctgtg tacacttctt atgtttttta cttctgataa atttttttga
     6061 aacatcatag aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga
     6121 ccgcaatttt tatttcttcg cacgtctggg cctctcatga cgtcaaatca tgctcatcgt
     6181 gaaaaagttt tggagtattt ttggaatttt tcaatcaagt gaaagtttat gaaattaatt
     6241 ttcctgcttt tgctttttgg ggtttcccct attgtttgtc aagatttcga ggacggcgtt
     6301 tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt
     6361 gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct
     6421 atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat aactgtttcc
     6481 tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     6541 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     6601 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     6661 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     6721 cgtaaggaga aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat
     6781 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     6841 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6901 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6961 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7021 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7081 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7141 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7201 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7261 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7321 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7381 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7441 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7501 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7561 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7621 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7681 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7741 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7801 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7861 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7921 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7981 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8041 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8101 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8161 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8221 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8281 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8341 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8401 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8461 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8521 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8581 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8641 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8701 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8761 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     8821 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8881 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8941 aaca
//
