LOCUS       sequence-354909-        8944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-2::Ca -insensitive D3cpv unc-54 3' UTR C.elegans neural 
            expression of Pacr-2 calcium D3cpv.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 8944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH2157
FEATURES             Location/Qualifiers
     source          1..8944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3622..3624,3625..3627,3628..4140)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             4846..5580
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     complement(6499..6521)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6621..6640
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6718..6736)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6804..6908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6909..6977,6978..7769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7127..7146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7940..8528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8429..8448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8682..8699
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8816..8837
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8852..8857,8858..8875,8876..8882)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8890..8906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8895..8917
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8914..8930
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8914..8930
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctgtaagtt taaacatgat cttactaact aactattctc atttaaattt tcagagctta
       61 aaaatggctg aaatcactca caacgatgga tacgctaaca acttggaaat gaaataagct
      121 tgggctgcag catgcggccg cattcttgga atttcgcgtt ttccattttc cgttgttttt
      181 tcattttata aaagtttcag cacctacatt tttcaatatt tcttcaatgg agaataaatc
      241 agttgaatca gttgaaaaac aaagtcagag ctgtggaagt cgaaatttaa gagctttttt
      301 tttaattttt tatagttgtt gtaaagctta ttagtatacc gcacctactg ttaatatgaa
      361 ccatcaaatg ttactcttcc tttttcatta aaaaaaactt tcagtttgcc ccgccggtgc
      421 gattccttat aagatgccac aagttttgtt atgcgacccg acaattgtta atatccgtcc
      481 atacgactat aaggtttggt tttattcgca ttttattgtt ttgtttttgg atcaactcta
      541 tcaaaaaaac aatattcgtt catgatacta atgactaatg agttttcagt gcgttgaagc
      601 agcaaacgga caatatcttc ctgccgatgg ccgatctctt tgctgcaaga ccaccactct
      661 gtactcgttt gcctcggttt ttggagaggc taaaataagc cctcggctag tgccaaatcc
      721 ccctctttca gcgatcgaat atgtgcgtcg gtattttttg ttcctcttat tttttgtctt
      781 agacatcaac gccgccgccg taaaacagaa gacatgtttc cgtacttcca ggtcactttg
      841 aacgttcaca catccgccaa agagcactca ccggaaatta gaactggaga tcattttgtg
      901 ctgacaccat acaaactcta cgagccggca tatttgaaaa aaatcaaact gttcacagac
      961 tttggtcatg gctcatttgt gcatgttata ctttttggta aatctattga acatgttcgg
     1021 agtgttcatg cgagcgtaac taagttttga gaaaaaaaga atgagagaat aactagggct
     1081 tttgtctttt tttgtgaata tctggttatg tttgaagatc caatgtcaac aactgaaaca
     1141 atgcaacttt attatgaccg accaacatcc aatgggaaaa ctatcaattt agatgagcca
     1201 attccagacg gaggtttcaa taattaaaaa aaaattaaga ataatcaaat aacatgcgct
     1261 tttcaggatt catttctaag aaaatattca atgcggctcc aattacacaa atagaaaacc
     1321 catcacgtcc gggtccaata aaagaatgtg agaactttct tcttgaaaaa tgaacatatt
     1381 gaattgcacc aaacgcaaac aagaaactct aaatttcaga tcgaaaattg tggattgtgc
     1441 tcattttcaa gaccacaagc ccgtttggtc gagtttatat tctttccacc aaggatttgc
     1501 acagcaagta tagatcagtc accgattttt tgaaatccga cacaggaaac atgctaggaa
     1561 ctcctgtggc tggaacttat ttttatgtga gttttgtttt gaaccctcaa ctataaaatt
     1621 gtcggcccgt tttgttttca gctcaccacc gactgatttt catttcaaaa ttaatgcaga
     1681 tttctacgtg ttctttttca aataaattta tatatttttt gttctctcac aaaataaata
     1741 atttcgagag ttaagtgatc attctcagac atattctata attgtccgtc aggaaacaga
     1801 tagaggaacc tttgataaat aaattataaa aggccgacag ggaaatgctt ctccctcgac
     1861 tctgttagaa gcgaagaggc aatttttaaa aatttcccat ttttatgcgt ttttctgatt
     1921 tccaaagtca cgcatcattt accaaccgca acgattcata aggacgtaat gttgattttc
     1981 tggacgattt ttatatagtt ttgatgtgaa attcgcttta tttgtctgaa agtggaacag
     2041 atttagtgtg gatttaaccg aaaaatgcag atcttttaaa aatttaaacc agtgttttgt
     2101 tgccttattt gcgaaaaaaa attgtgatat ttcgagtatt gctgcaattt gattagtaaa
     2161 atgttaggat ataagtattt aaaaagtata attggttgaa ttttcttttt catcaccgac
     2221 ttccaaaaat atagagatac tgagttttca ccattttttg aatgtaaata aaaacatttt
     2281 gaacacttgt gctgggattc aatccaaata cattcactgt catttaatat tattatatat
     2341 caaaataaaa cgcataactt tttctgtttt tttttaaatt ttttcaaaat gtaggtttgg
     2401 taagttaagg taaggttagg caatgtaggt aaacacgaga tgatactcca cgttgaaaat
     2461 tgttttcata aatacagttt agattgaact gaaggatttt atcgtctaat agaatataat
     2521 tttttagttc aagatttagg atttgttttc actttttttt agattcaaga catatatctc
     2581 gcaaaatgag ttttgtcttt gagccatctt gtcatcatag cacattgaat tttgttgttc
     2641 gcaagacgga atatatgttc ccagctgcaa attttgtgtc ggatatttct tctttagcga
     2701 aaggcgttaa aaaccaaaaa tagtcgaagc gtttgatatg tgtcccgttg gtttttcgtg
     2761 tccatctatt ctgtttcgag tacacatgtt gcgacgcggc actgatgaaa aagtgaaaga
     2821 gctagtgtca cacacaaaca ctaagtatgc tcatgtgagt gtgtagatat gtgtagatgt
     2881 atgtcctgaa ggaataaaaa gactttttga agctacattt ttaaacaaaa aaagacagaa
     2941 acatgaagca caactctcta ttctgtcgaa cggcgtgtgc aactcacgca cacccaaaaa
     3001 tatgaaatat aacattcgca ccaacaaagc gcgaatatca atgaatctcg agggaacgat
     3061 ggctgcctgc cggcgcgtgt tgctcttctg tcattgggat aactggcggg tctgttgggt
     3121 ccgtccttta gaccatttga ctacactgcc ctgcatatgt gtgaaacgtc gtcgttgctg
     3181 ttgggagctg tctgtgtgta tgtgtgtgtg tgtgagtatg gagcagtaaa aagctgccga
     3241 tacgagaagc gtcgccccgg acggacggcg atggcgcatc gccaaatgtg tcattttagt
     3301 tgtcttcacg aaccagaggg cccacgacgg acgggcggcc acgcccacct caaccagaat
     3361 atccttcctt ctcccaccca cccttgcgga cgcgcggatg cgctgaacaa aaaggcgacg
     3421 aagaagaaga agaagaagct gaacgaagaa aaaagcatca aaagaagtga agcggctcta
     3481 ccaccccgac caccttcctc cacaggaagg acgccgtttt catcccggga ttggccaaag
     3541 gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca ggaggaccct
     3601 tggctagcgt cgacggatcc catggtgagc aagggcgagg agctgttcac cggggtggtg
     3661 cccatcctgg tcgagctgga cggcgacgta aacggccaca ggttcagcgt gtccggcgag
     3721 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3781 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc
     3841 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3901 gtccaggagc gtaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     3961 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4021 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc
     4081 accgccgaca agcagaagaa cggcatcaag gcccacttca agatccgcca caacatcgag
     4141 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4201 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4261 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgcccgcat gcatgaccaa
     4321 ctgacagaag agcagattgc agagttcaaa gaagccttct cattattgga caaggatggg
     4381 gacggcacca tcaccacaaa gcagctgggc accgctctga ggtcgcttgg acaaaaccca
     4441 acggaagcag aattgcagga tatgatcaat gaagtcgatg ctgatggcaa tggaacgatt
     4501 tactttcctc aatttcttac tatgatggct agaaaaatga aggacacaga cagcgaagag
     4561 gaaatccgag aagcattccg tgtttttgac aaggatggga acggctacat cagcgctgct
     4621 caattgcgtc acgtcatgac aaacctcggg gagaagttaa cagatgaaga agttgatgaa
     4681 atgataaggg aagcagatat cgatggtgat ggccaagtaa actatgaaca gtttgtacaa
     4741 atgatgacag caaagggggg gaagaggcgc tggcagaaaa caggccatgc tgtccgagca
     4801 ttcggaagac tgaagaagat ctccagctcc ggggcactgg agctcatgga cggcggcgtg
     4861 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4921 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     4981 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     5041 tacaagggtg gcagcggtgg catggtgagc aagggcgagg agctgttcac cggggtggtg
     5101 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     5161 ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag
     5221 ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc
     5281 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     5341 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     5401 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     5461 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     5521 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     5581 taagaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa
     5641 taggggccgc tgtcatcaga gtaagtttaa actgagttct actaactaac gagtaatatt
     5701 taaattttca gcatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat
     5761 ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt
     5821 ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag
     5881 attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca
     5941 ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt
     6001 acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat
     6061 atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg
     6121 acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag
     6181 tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt
     6241 caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca
     6301 agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa
     6361 tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat
     6421 ataccaaaca taactgtttc ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt
     6481 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     6541 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     6601 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     6661 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc
     6721 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     6781 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6841 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6901 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6961 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7021 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7081 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7141 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7201 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7261 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7321 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7381 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7441 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7501 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7561 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7621 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7681 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7741 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7801 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7861 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7921 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7981 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8041 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8101 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8161 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8221 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8281 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8341 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8401 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8461 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8521 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8581 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8641 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8701 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8761 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     8821 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     8881 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     8941 ccaa
//
