LOCUS       sequence-354911-        9854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-2::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression
            of UNC-7-wCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH1898
FEATURES             Location/Qualifiers
     source          1..9854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    4..20
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9..31
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     28..44
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     28..44
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(4804..4815)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     primer_bind     complement(7467..7489)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7589..7608
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7686..7704)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7772..7876
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7877..7945,7946..8737)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8095..8114)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8908..9496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9397..9416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9650..9667
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9784..9805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9820..9825,9826..9843,9844..9850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
       61 tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa
      121 aatggctgaa atcactcaca acgatggata cgctaacaac ttggaaatga aataagcttg
      181 ggctgcagca tgcggccgca ttcttggaat ttcgcgtttt ccattttccg ttgttttttc
      241 attttataaa agtttcagca cctacatttt tcaatatttc ttcaatggag aataaatcag
      301 ttgaatcagt tgaaaaacaa agtcagagct gtggaagtcg aaatttaaga gctttttttt
      361 taatttttta tagttgttgt aaagcttatt agtataccgc acctactgtt aatatgaacc
      421 atcaaatgtt actcttcctt tttcattaaa aaaaactttc agtttgcccc gccggtgcga
      481 ttccttataa gatgccacaa gttttgttat gcgacccgac aattgttaat atccgtccat
      541 acgactataa ggtttggttt tattcgcatt ttattgtttt gtttttggat caactctatc
      601 aaaaaaacaa tattcgttca tgatactaat gactaatgag ttttcagtgc gttgaagcag
      661 caaacggaca atatcttcct gccgatggcc gatctctttg ctgcaagacc accactctgt
      721 actcgtttgc ctcggttttt ggagaggcta aaataagccc tcggctagtg ccaaatcccc
      781 ctctttcagc gatcgaatat gtgcgtcggt attttttgtt cctcttattt tttgtcttag
      841 acatcaacgc cgccgccgta aaacagaaga catgtttccg tacttccagg tcactttgaa
      901 cgttcacaca tccgccaaag agcactcacc ggaaattaga actggagatc attttgtgct
      961 gacaccatac aaactctacg agccggcata tttgaaaaaa atcaaactgt tcacagactt
     1021 tggtcatggc tcatttgtgc atgttatact ttttggtaaa tctattgaac atgttcggag
     1081 tgttcatgcg agcgtaacta agttttgaga aaaaaagaat gagagaataa ctagggcttt
     1141 tgtctttttt tgtgaatatc tggttatgtt tgaagatcca atgtcaacaa ctgaaacaat
     1201 gcaactttat tatgaccgac caacatccaa tgggaaaact atcaatttag atgagccaat
     1261 tccagacgga ggtttcaata attaaaaaaa aattaagaat aatcaaataa catgcgcttt
     1321 tcaggattca tttctaagaa aatattcaat gcggctccaa ttacacaaat agaaaaccca
     1381 tcacgtccgg gtccaataaa agaatgtgag aactttcttc ttgaaaaatg aacatattga
     1441 attgcaccaa acgcaaacaa gaaactctaa atttcagatc gaaaattgtg gattgtgctc
     1501 attttcaaga ccacaagccc gtttggtcga gtttatattc tttccaccaa ggatttgcac
     1561 agcaagtata gatcagtcac cgattttttg aaatccgaca caggaaacat gctaggaact
     1621 cctgtggctg gaacttattt ttatgtgagt tttgttttga accctcaact ataaaattgt
     1681 cggcccgttt tgttttcagc tcaccaccga ctgattttca tttcaaaatt aatgcagatt
     1741 tctacgtgtt ctttttcaaa taaatttata tattttttgt tctctcacaa aataaataat
     1801 ttcgagagtt aagtgatcat tctcagacat attctataat tgtccgtcag gaaacagata
     1861 gaggaacctt tgataaataa attataaaag gccgacaggg aaatgcttct ccctcgactc
     1921 tgttagaagc gaagaggcaa tttttaaaaa tttcccattt ttatgcgttt ttctgatttc
     1981 caaagtcacg catcatttac caaccgcaac gattcataag gacgtaatgt tgattttctg
     2041 gacgattttt atatagtttt gatgtgaaat tcgctttatt tgtctgaaag tggaacagat
     2101 ttagtgtgga tttaaccgaa aaatgcagat cttttaaaaa tttaaaccag tgttttgttg
     2161 ccttatttgc gaaaaaaaat tgtgatattt cgagtattgc tgcaatttga ttagtaaaat
     2221 gttaggatat aagtatttaa aaagtataat tggttgaatt ttctttttca tcaccgactt
     2281 ccaaaaatat agagatactg agttttcacc attttttgaa tgtaaataaa aacattttga
     2341 acacttgtgc tgggattcaa tccaaataca ttcactgtca tttaatatta ttatatatca
     2401 aaataaaacg cataactttt tctgtttttt tttaaatttt ttcaaaatgt aggtttggta
     2461 agttaaggta aggttaggca atgtaggtaa acacgagatg atactccacg ttgaaaattg
     2521 ttttcataaa tacagtttag attgaactga aggattttat cgtctaatag aatataattt
     2581 tttagttcaa gatttaggat ttgttttcac ttttttttag attcaagaca tatatctcgc
     2641 aaaatgagtt ttgtctttga gccatcttgt catcatagca cattgaattt tgttgttcgc
     2701 aagacggaat atatgttccc agctgcaaat tttgtgtcgg atatttcttc tttagcgaaa
     2761 ggcgttaaaa accaaaaata gtcgaagcgt ttgatatgtg tcccgttggt ttttcgtgtc
     2821 catctattct gtttcgagta cacatgttgc gacgcggcac tgatgaaaaa gtgaaagagc
     2881 tagtgtcaca cacaaacact aagtatgctc atgtgagtgt gtagatatgt gtagatgtat
     2941 gtcctgaagg aataaaaaga ctttttgaag ctacattttt aaacaaaaaa agacagaaac
     3001 atgaagcaca actctctatt ctgtcgaacg gcgtgtgcaa ctcacgcaca cccaaaaata
     3061 tgaaatataa cattcgcacc aacaaagcgc gaatatcaat gaatctcgag ggaacgatgg
     3121 ctgcctgccg gcgcgtgttg ctcttctgtc attgggataa ctggcgggtc tgttgggtcc
     3181 gtcctttaga ccatttgact acactgccct gcatatgtgt gaaacgtcgt cgttgctgtt
     3241 gggagctgtc tgtgtgtatg tgtgtgtgtg tgagtatgga gcagtaaaaa gctgccgata
     3301 cgagaagcgt cgccccggac ggacggcgat ggcgcatcgc caaatgtgtc attttagttg
     3361 tcttcacgaa ccagagggcc cacgacggac gggcggccac gcccacctca accagaatat
     3421 ccttccttct cccacccacc cttgcggacg cgcggatgcg ctgaacaaaa aggcgacgaa
     3481 gaagaagaag aagaagctga acgaagaaaa aagcatcaaa agaagtgaag cggctctacc
     3541 accccgacca ccttcctcca caggaaggac gccgttttca tcccgggatt ggccaaagga
     3601 cccaaaggta tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg
     3661 gctagcgtcg acggatcccc atgctcggct cctccagcaa tcctgaaccg ccactcctgt
     3721 cgcggataat cggagtacct ccaccgccgc cgccacgagc accgaccaca gctctcgtcc
     3781 ttcgggtgcc caccgtcgac agtccatcga aaaagaaaca acagccagac actaggaaca
     3841 aatatcagga aactgcgctc agggataaaa aaactcggac accacttgaa aaagccagac
     3901 atctcgacaa tcttccctcg tatcaggccc aaaaactatt agacggttcg caccagcttc
     3961 gcatcgactc ccaccacgtt ggtagtgcag gtcacggtgc aggacaaggt cacggacaca
     4021 agaaagagtt tggacctgca atgattctct attatttagc ttctgctttt cgggcgctct
     4081 atccgcgtct agatgatgat tttgtggaca aacttaatta ttattatact actacaatat
     4141 tagcttcctt tgcacttctt gtttccgcca agcagtatgt cggttttccc atccaatgtt
     4201 gggtgcccgc cacattcaca gatgccatgg agcagtacac tgagaactac tgctgggtac
     4261 agaacacata ctgggttccc atgcaagagg acattccacg tgaaatttat tcgcgtagaa
     4321 atcgacaaat tggctattac caatgggtgc cgtttattct tgctattgag gccttgttgt
     4381 tctacgttcc atgcattctc tggagaggat tgttgtattg gcacagtggt atcaacctac
     4441 aagggctagt ccagatggcg tgtgacgcac gactgatgga ttcggaaata aagacgagaa
     4501 cagtctacac aatggcccgg cacatgcaag acgaggttca gctcaccaat atcgaccggc
     4561 aaggacacag tcggagttgt ttctcgaatt tgcaacttgg cgcaaactgt gggagacatt
     4621 gtggttgcta cgtcactatg ctctacatag gaatcaaggt tctctactca gcaaatgtgt
     4681 tgctccagtt tttcttgctg aaccatcttc ttgggtctaa cgacttggct tatggattct
     4741 ctttgttgaa agacttgatg cacgcgattg agtgggaaca gactggaatg ttccccaggg
     4801 tcacactttg tgattttgag gttcgagttc tgggaaacat tcatcgacat accgttcagt
     4861 gcgtgttaat gatcaatatg tttaatgaga aaattttctt attcctctgg ttttggttct
     4921 taacctgtgg aattgttacc gtatgtaaca ctatgtactg gattctcatt atgttcattc
     4981 cgtctcaggg aatgtcattt gtccgcaaat acctccgtgt tctacctgac caccctgcga
     5041 aaccaatagc tgatgacgtc acgctgagga agttcactaa taacttcctc cgcaaagatg
     5101 gagtcttcat gttgcgaatg atttccacac acgccggcga actgatgagc agtgagctca
     5161 ttcttgctct ctggcaggat ttcaacaacg tcgaccgcag tccgacgcaa ttttgggatg
     5221 cggaacacgg tcaagggacg atagactgac ggatccccga gatcttcagc gctcagcatt
     5281 cgtagaattg taagtttaaa ctttctcata ctaactaacg cgctctattt aaattttcag
     5341 aattcgctgt ctcatcctac tttcacctag ttaactgctt gtcttaaaat ctatgcttct
     5401 ctttagtatc taaaattttc ctagaagctt acaagtatat aaatggtctc ttctcaataa
     5461 aggttgtata tttattcatc ttattgaatc tgccatttcc tcgtttttgc gagtttatat
     5521 accttccaat tttctttcta ttgtattttc aacttctaat tttaattcag ggaaactgct
     5581 tcaacgcatc ggtaccctat ggtctcaaag ggtgaagaag ataacatggc aattattaaa
     5641 gagtttatgc gtttcaaggt gcatatggag ggatctgtca atgggcatga gtttgaaatt
     5701 gaaggtgaag gagaaggccg accatatgag ggaacacaaa ccgcaaaact aaaggtaagt
     5761 ttaaacatat atatactaac taaccctgat tatttaaatt ttcaggtaac taaaggcgga
     5821 ccattaccat tcgcctggga catcctctct ccacagttca tgtatggaag taaagcttat
     5881 gttaaacatc cggcagatat accagattat ttgaaacttt cattcccgga gggttttaag
     5941 tgggaacgcg taatgaattt tgaagacgga ggagttgtta cagtgacgca agactcaagg
     6001 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag cctccaagat
     6061 ggagaattta tttataaagt caaacttcga ggaacgaatt tcccctcgga tggatctgtt
     6121 atgcagaaga agactatggg atgggaagct tcaagtgaaa gaatgtaccc tgaagacggt
     6181 gctcttaagg gagagattaa acaacgtctt aaattgaaag atggaggaca ttacgatgct
     6241 gaggtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcaggtgaag
     6301 acaacttaca aagccaaaaa accagttcag ctgccaggag cgtacaatgt taatattaaa
     6361 ctggatatca cctcccacaa cgaggattac actatcgttg agcaatatga aagagctgaa
     6421 gggcggcact cgacaggtgg catggatgaa ttgtataagt aggagctccc gagatcttca
     6481 gcgctcagca ttcgtagaat tgtaagttta aactttctca tactaactaa cgcgctctat
     6541 ttaaattttc agaattccaa ctgagcgccg gtcgctacca ttaccaactt gtctggtgtc
     6601 aaaaataata ggggccgctg tcatcagagt aagtttaaac tgagttctac taactaacga
     6661 gtaatattta aattttcagc atctcgcgcc cgtgcctctg acttctaagt ccaattactc
     6721 ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca
     6781 aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa
     6841 ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc
     6901 tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta
     6961 tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac
     7021 cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc
     7081 ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt
     7141 caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta
     7201 ttgtttgtca agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc
     7261 acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa
     7321 gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca
     7381 ttcccaatat accaaacata actgtttcct actagtcggc cgtacgggcc ctttcgtctc
     7441 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     7501 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     7561 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     7621 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct
     7681 taagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     7741 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7801 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7861 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7921 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7981 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     8041 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     8101 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     8161 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     8221 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     8281 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     8341 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     8401 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8461 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8521 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8581 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8641 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8701 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8761 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8821 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8881 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8941 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     9001 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     9061 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     9121 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     9181 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     9241 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     9301 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     9361 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9421 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9481 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9541 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9601 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9661 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     9721 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9781 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9841 tcgtatgttg tgtg
//
