LOCUS       sequence-354912-        9175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pnmr-1::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression
            of UNC-9-wCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 9175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH2069
FEATURES             Location/Qualifiers
     source          1..9175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    93..114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(129..134,135..152,153..159)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    167..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     172..194
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     191..207
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     191..207
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6951..6973)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7073..7092
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7170..7188)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7256..7360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7361..7429,7430..8221)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7579..7598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8392..8980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8881..8900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9134..9151
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
       61 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      121 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      181 caatttcaca caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga
      241 tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc
      301 acaacgatgg atacgctaac aacttggaaa tgaaataagc ttgggctgca gcatgcggcc
      361 gctgtggtct cactttgaac gacaaaagaa aaccaaatat tgtaaaggaa tagtacagat
      421 aaaagggaac aagtgatacc agctgatgga tgtaggtcag cgacaaaaga caggagacgt
      481 atagtttgat gattatggaa ccaaactcag aatttaatgt tggagacgct ataaaaattg
      541 aattgagaaa ttgaaaccat attcttgaat atctctcaat ttggaatttg gaagtagccg
      601 taaaatcggc tagctagaac ataaaaggac tgatttgcaa ccttgaccat tcatgatatc
      661 atttcaataa aagaagagat tccaatgacc aatcttcaag aatcacaaca aaactaagca
      721 aaagtaacaa caaattgttc aggtagacaa gtagacattt ggacacattt tgcggggatg
      781 tgatgactgt ataataaaaa taagtggaca ctgagagaga gaggaggtca gcaggaaaaa
      841 agaacatcga actgcttgac gaattgaaat tagaatgagg tgtcaggcag gtgacgatta
      901 catggttaat tattagtgtt ctttttcttc tttttgtgga tgccgattag ttgaaataat
      961 tgcatggaaa gtgagaattc aatgtgaatc tctttcttat tatgtttcaa gtggtatctg
     1021 gatggaagct tgtactttta tgaatcgaaa attgcgccca aggttgattt tgagattatt
     1081 cagtaaattt ttgtgctgat tattcagtta atgtagaaca gagacgcgga tgaaaacttt
     1141 tttaatttct aaaaaaaaag acaaaatgtg gttaaaaagt ttggaaaata attgttatat
     1201 acttcttgag agcttattca aacggaaacc aatcataaat agtcgaatag tcatttagaa
     1261 aagagaaact agttgatgta gagagattca aaaagtaaaa tatgcaaatg atccgtttct
     1321 tagatttagt aaacatctac caaatgaaac aggaattgtc gaaacaattt agaaaaggag
     1381 aaggaaacat ttgtttattt tcccctttaa atggcggtag aggtaaaaca gcaatacaca
     1441 tagtacaagg ttatactact ttcaaaagaa tttgacatcc cgccgagaaa atgtggtgat
     1501 tatatggtaa agttttcagt tggatattat cttctacaga cggtgattca aaatgatcaa
     1561 aagggcaagt gggtggtagg atttgaaaaa ttgagatata gaacgtgcga ttttggtaaa
     1621 actttttgaa cacaaggcat atttttgaac tttgaaaaac gcaaaattta aattttgtta
     1681 attacatata ttttgaacag attatgaata taagcaaggc tacataaaat atgtatattt
     1741 tctttttaca aatattcgta cactgttaaa tttatagaaa aatcatttat attcagaaaa
     1801 acctatttga acaattttta gttgctcaat aaccaatttg cacaaattta gaaaatataa
     1861 tgggggcttt taaaaattta aatcctcagc gaatattcct ttgcgatttt ttcgaacgtt
     1921 ttctttcaga ttaaaaaatc tgaagcagaa gtaaatgaac taccatagaa aattcaccaa
     1981 ggatcacatt tttacaaagc ctccgcccaa ttgtaaactt gggaattttt gcttgttttc
     2041 tttttttttt caaaaaagga atagttgttt taaagtaatt tattccccta tgctttctcc
     2101 agcaatcagt tgttctcccc atactttaca caattatcaa caacttcaca tagaaaacaa
     2161 aacaacaaat cagaaattcc caccggtcac agtcacagat tccccatgtt ttgttcatct
     2221 catttctgca agaaagtgac atcgctgcca cccaattcga caaattgtcc aagtggtcat
     2281 caggaagata tgaccttctt tttaatgata atttagacta caaagggcgt caaaaaagat
     2341 tcaatgatca ggggtaattg ctgtaaaatg tagtttgtat gtgtgtttac cgtagcctat
     2401 aactgtaaaa attggttttt taaaaagtat ttctgagatt aaaaggctcc ccaaggatgc
     2461 acgattctag ctagaaatga acattccatg ggttgtgaag atgcttttat ctcactttag
     2521 aatgtctgac tccgcccctc tgtgtacgaa ttgaagtgct cttttgtgat gatcatagct
     2581 cctccttgcg gcgacgcccg cacacagcac gagatattac acggaatact tatgtgattc
     2641 ataggaaaca aggaaatcga aaaaataata ggattttgag gaggtatttt tagatgaatg
     2701 cttatgatgt tatgatgaac ccaagaaatt ctgaatagac atatgtcaga acggggtagg
     2761 agctccagtg tcgcttttga aataaaaaat caagtggtat acttctcgta tatacccttc
     2821 tgaactaata aaataattta tcttttgaaa aagtttgaaa aagaaaaatg cccaaaactt
     2881 gaattttaat gttttgtatt ttgagcatat cgttcaaacg tcgtgatctg aaagtagaaa
     2941 gatctaaata ttcgtgacac agtttggttt gaaggtctta gtggtaattt tttattacag
     3001 aagcaacaaa aagttgacat attttcagaa tttccaaaaa aacgtttaaa atttcaattt
     3061 cttcgataga gttctaaaaa ttttttggaa cggattttgt aatatccgct gataaccgtt
     3121 caagaacaat ttgaattttg aaataattta atcaactagt atccagtgct ctttctcact
     3181 gctccaccca tttttcaacc catttccttt actcaatttc gcccgttttc cttttgctca
     3241 aatgtgccca ccaaatcatc tttcttcgtc ttcttttctc ttattcggaa ggagaagagg
     3301 tgaaagtaga ataagttttt ttgttgaaaa gagaggagca cggccacttt tctcttattt
     3361 ccatccttct actacctcgt tttactttcc atttaaaaac ccgtttaaag ttccaaaatt
     3421 tcaattatct tgcttctgtt tggaacacga caaactttag ttttgttaca gatacccggg
     3481 attggccaaa ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc
     3541 aggaggaccc ttggctagcg tcgacggatc cggatgagta tgctattgta ttatttcgcg
     3601 agtgctgtga agtcaataca gtttcacgtc gatgatgaca ttattgacaa gcttaactac
     3661 tattacacca cagccattat cacagtgttc gcgattctcg tctctgctaa acagtatgtc
     3721 ggttttccaa tccaatgttg ggtacctgcg acgttcaccg agcccatgga gcagtacacg
     3781 gaaaactatt gttgggttca aaacacctac tttctccctt tacacgacta cattccacat
     3841 aactatgcag aacgtgaaaa tcgacaaatc gggtattatc aatgggttcc gtttgtgtta
     3901 gctcttgaag ctcttctttt ctatgtgccg actattgtat ggagactgct cagctggcaa
     3961 tcaggaattc atgtgcagtc tctggttcaa atggcttgtg actccagatt gttggatttg
     4021 gaatcgagga acagagcgtt gcaaaccatc gcaacaaatg tagaagaagc gctacacgtg
     4081 aaacatcaag tgatgtccgg aaatcgtctg aaactgctca acttgattat ttgcacacga
     4141 tcttccggtg ctgctgtcac gttcctctat atctctgtca aaattctgta caccgtgaac
     4201 atcgtcggcc aaatctttct actgaacacg tttctcggta accgtagcaa atggtacgga
     4261 ctgcaagttc tcaacgactt gatgaacgga cgagaatggg aggagtctgg tcattttcca
     4321 cgtgtaacgt tatgtgactt tgaagtaaaa gtactgggaa acgtgcaccg acatacagta
     4381 caatgtgtac ttatgattaa catgttcaac gagaagatct ttctgttctt gtggttctgg
     4441 tacttccttc ttgccggtgc tactttgtgc tcgcttttct actggatcta catttcagtg
     4501 gtgccaagca ggcagctcaa ctttgttgga aagtatctga ctggaatcga gggatataaa
     4561 atggtggatt cgcaatcact acgacgtttt gtatttcact ttttaagaca ggatggtgtt
     4621 ttcctacttc gtatggttgc aactcacgcc ggagaactac cctgttacga gcttgcgaaa
     4681 acgttatgga acaactactg tgacaataaa gaaggaaaaa tgcacgacgt gtgaggatcc
     4741 ccgagatctt cagcgctcag cattcgtaga attgtaagtt taaactttct catactaact
     4801 aacgcgctct atttaaattt tcagaattcg ctgtctcatc ctactttcac ctagttaact
     4861 gcttgtctta aaatctatgc ttctctttag tatctaaaat tttcctagaa gcttacaagt
     4921 atataaatgg tctcttctca ataaaggttg tatatttatt catcttattg aatctgccat
     4981 ttcctcgttt ttgcgagttt atataccttc caattttctt tctattgtat tttcaacttc
     5041 taattttaat tcagggaaac tgcttcaacg catcggtacc ctatggtctc aaagggtgaa
     5101 gaagataaca tggcaattat taaagagttt atgcgtttca aggtgcatat ggagggatct
     5161 gtcaatgggc atgagtttga aattgaaggt gaaggagaag gccgaccata tgagggaaca
     5221 caaaccgcaa aactaaaggt aagtttaaac atatatatac taactaaccc tgattattta
     5281 aattttcagg taactaaagg cggaccatta ccattcgcct gggacatcct ctctccacag
     5341 ttcatgtatg gaagtaaagc ttatgttaaa catccggcag atataccaga ttatttgaaa
     5401 ctttcattcc cggagggttt taagtgggaa cgcgtaatga attttgaaga cggaggagtt
     5461 gttacagtga cgcaagactc aaggtaagtt taaacagttc ggtactaact aaccatacat
     5521 atttaaattt tcagcctcca agatggagaa tttatttata aagtcaaact tcgaggaacg
     5581 aatttcccct cggatggatc tgttatgcag aagaagacta tgggatggga agcttcaagt
     5641 gaaagaatgt accctgaaga cggtgctctt aagggagaga ttaaacaacg tcttaaattg
     5701 aaagatggag gacattacga tgctgaggta agtttaaaca tgattttact aactaactaa
     5761 tctgatttaa attttcaggt gaagacaact tacaaagcca aaaaaccagt tcagctgcca
     5821 ggagcgtaca atgttaatat taaactggat atcacctccc acaacgagga ttacactatc
     5881 gttgagcaat atgaaagagc tgaagggcgg cactcgacag gtggcatgga tgaattgtat
     5941 aagtaggagc tcccgagatc ttcagcgctc agcattcgta gaattgtaag tttaaacttt
     6001 ctcatactaa ctaacgcgct ctatttaaat tttcagaatt ccaactgagc gccggtcgct
     6061 accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca gagtaagttt
     6121 aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg cgcccgtgcc
     6181 tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc
     6241 caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct
     6301 tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa
     6361 aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac
     6421 acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca
     6481 tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat
     6541 ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag
     6601 ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc
     6661 ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc gtttttcttg
     6721 ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg
     6781 aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt
     6841 ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt tcctactagt
     6901 cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     6961 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     7021 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     7081 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     7141 agaaaatacc gcatcaggcg gccttaaggg cctcgtgata cgcctatttt tataggttaa
     7201 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7261 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7321 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7381 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7441 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7501 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7561 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7621 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7681 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7741 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7801 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7861 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7921 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7981 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8041 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8101 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8161 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8221 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8281 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8341 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8401 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8461 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8521 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8581 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8641 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8701 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8761 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8821 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8881 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8941 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9001 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9061 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9121 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgc
//
