LOCUS       sequence-354919-       11937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor plasmid for cardiac-specific CCND2-T2A-Chloc-P2A-eGFP 
            expression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11937)
  AUTHORS   Joshi J, Xu B, Rubart M, Chang Y, Bao X, Chaliki HP, Scott LR, Zhu W
  TITLE     Optogenetic Control of Engrafted Human Induced Pluripotent Stem 
            Cell-Derived Cardiomyocytes in Live Mice: A Proof-of-Concept Study.
  JOURNAL   Cells. 2022 Mar 10;11(6). pii: cells11060951. doi: 
            10.3390/cells11060951.
  PUBMED    35326403
REFERENCE   2  (bases 1 to 11937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2022-03-10"; volume: "11(6). pii: cells11060951. doi"; pages:
            " 10.3390/cells11060951"
COMMENT     Sequence Label: AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP
FEATURES             Location/Qualifiers
     source          1..11937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..836
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    843..868
                     /label=SA
                     /note="splice acceptor site"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             955..1554
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(955..974)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1451..1471
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1586..1603)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1592..1816
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2460..2478
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2510..2534
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2547..2556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3429..3482
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3483..4409
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEQIYVCAIRMVKVILEFFFSFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIHLSNLTGLSNDYSRRTMGLLVSNIGCIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             4425..4481
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4482..4484,4485..4487,4488..5198)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4482..4484,4485..4487,4488..5198)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4527..4548)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4788..4807)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5135..5156
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5199..5219
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5294..5882
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5347..5367)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5765..5776)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5914..6390
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6113..6132)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6500..6519)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6565..6620
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6619..6638)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6981..6997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7005..7021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7029..7035,7036..7053,7054..7059))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7074..7095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    7169..8005
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(8040..8058)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8065..8082)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8065..8081)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8074..8096)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8219..8521
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8400..8419
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(8924..8943)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9538..9557
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9918..10709,10710..10778))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10541..10560
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10902..11490
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11391..11410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11644..11661
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11778..11799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11814..11819,11820..11837,11838..11844)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11852..11868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11876..11892
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11913..11931
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagatgacc
      961 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     1021 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     1081 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     1141 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     1201 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     1261 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     1321 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     1381 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     1441 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     1501 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatctaga
     1561 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     1621 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1681 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1741 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1801 gcggtgggct ctatgggtct cgacattgat tattgactag tgtcatggag aagacccacc
     1861 ttgcagatgt cctcactggg gctggcagag ccggcaacct gcccaaggct gctcagtcca
     1921 ttaggagcca gtagcctgga agatgtcttt acccccagca tcagttcaag tggagcagca
     1981 cataactctt gccctctgcc ttccaagatt ctggtgctga gacttatgga gtgtcttgga
     2041 ggttgccttc tgccccccaa ccctgctccc agctggccct cccaggcctg ggttgctggc
     2101 ctctgcttta tcaggattct caagagggac agctggttta tgttgcatga ctgttccctg
     2161 catatctgct ctggttttaa atagcttatc tgagcagctg gaggaccaca tgggcttata
     2221 tggcgtgggg tacatgttcc tgtagccttg tccctggcac ctgccaaaat agcagccaac
     2281 accccccacc cccaccgcca tccccctgcc ccacccgtcc cctgtcgcac attcctccct
     2341 ccgcagggct ggctcaccag gccccagccc acatgcctgc ttaaagccct ctccatcctc
     2401 tgcctcaccc agtccccgct gagactgagc agacgcctcc aggatccgcc accctcgagt
     2461 aatacgactc actataggga gacccaagct ggctaggtaa gcttgatcaa caagtttgta
     2521 caaaaaagca ggctgtcgac ggtaccgcca ccatggagct gctgtgccac gaggtggacc
     2581 cggtccgcag ggccgtgcgg gaccgcaacc tgctccgaga cgaccgcgtc ctgcagaacc
     2641 tgctcaccat cgaggagcgc taccttccgc agtgctccta cttcaagtgc gtgcagaagg
     2701 acatccaacc ctacatgcgc agaatggtgg ccacctggat gctggaggtc tgtgaggaac
     2761 agaagtgcga agaagaggtc ttccctctgg ccatgaatta cctggaccgt ttcttggctg
     2821 gggtcccgac tccgaagtcc catctgcaac tcctgggtgc tgtctgcatg ttcctggcct
     2881 ccaaactcaa agagaccagc ccgctgaccg cggagaagct gtgcatttac accgacaact
     2941 ccatcaagcc tcaggagctg ctggagtggg aactggtggt gctggggaag ttgaagtgga
     3001 acctggcagc tgtcactcct catgacttca ttgagcacat cttgcgcaag ctgccccagc
     3061 agcgggagaa gctgtctctg atccgcaagc atgctcagac cttcattgct ctgtgtgcca
     3121 cggactttaa gtttgccatg tacccaccgt cgatgatcgc aactggaagt gtgggagcag
     3181 ccatctgtgg gctccagcag gatgaggaag tgagctcgct cacttgtgat gccctgactg
     3241 agctgctggc taagatcacc aacacagacg tggattgtct caaagcttgc caggagcaga
     3301 ttgaggcggt gctcctcaat agcctgcagc agtaccgtca ggaccaacgt gacggatcca
     3361 agtcggagga tgaactggac caagccagca cccctacaga cgtgcgggat atcgacctgg
     3421 gcagcggaga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
     3481 ctatggatta tggaggcgcc ctgagtgccg ttgggcgcga gctgctattt gtaacgaacc
     3541 cagtagtcgt caatggctct gtacttgtgc ctgaggacca gtgttactgc gcgggctgga
     3601 ttgagtcgcg tggcacaaac ggtgcccaaa cggcgtcgaa cgtgctgcaa tggcttgctg
     3661 ctggcttctc catcctactg cttatgtttt acgcctacca aacatggaag tcaacctgcg
     3721 gctgggagca gatctatgtg tgcgctatca ggatggtcaa ggtgattctc gagttcttct
     3781 tctcgtttaa gaacccgtcc atgctgtatc tagccacagg ccaccgcgtc cagtggttgc
     3841 gttacgccga gtggcttctc acctgcccgg tcattctcat tcacctgtca aacctgacgg
     3901 gcttgtccaa cgactacagc aggcgcacca tgggtctgct tgtgtctaat attggctgta
     3961 ttgtgtgggg cgccacttcc gccatggcca ccggatacgt caaggtcatc ttcttctgcc
     4021 tgggtctgtg ttatggtgct aacacgttct ttcacgctgc caaggcctac atcgagggtt
     4081 accacaccgt gccgaagggc cggtgtcgcc aggtggtgac tggcatggct tggctcttct
     4141 tcgtatcatg gggtatgttc cccatcctgt tcatcctcgg ccccgagggc ttcggcgtcc
     4201 tgagcgtgta cggctccacc gtcggccaca ccatcattga cctgatgtcg aagaactgct
     4261 ggggtctgct cggccactac ctgcgcgtgc tgatccacga gcatatcctc atccacggcg
     4321 acattcgcaa gaccaccaaa ttgaacattg gtggcactga gattgaggtc gagacgctgg
     4381 tggaggacga ggccgaggct ggcgcggtag ctagcggcag cggcgccact aacttctccc
     4441 tgttgaaaca agcaggggat gtcgaagaga atcccgggcc aatggtgagc aagggcgagg
     4501 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     4561 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     4621 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     4681 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     4741 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     4801 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     4861 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     4921 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     4981 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     5041 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     5101 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     5161 ccgccgggat cactctcggc atggacgagc tgtacaagcc aaagaagaag cgtaaggtat
     5221 aagtacaagt aagaattccg atcatattca ataacccact agtacgcgtg aattcgatat
     5281 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5341 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5401 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5461 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5521 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5581 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5641 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     5701 tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5761 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5821 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5881 gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga cccctcccca
     5941 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     6001 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     6061 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     6121 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     6181 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     6241 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     6301 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     6361 gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccag gcgcgtgaat
     6421 tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg
     6481 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     6541 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     6601 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     6661 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     6721 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     6781 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     6841 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     6901 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
     6961 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7021 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7081 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7141 tgccagcgga tcgacagtac taagctttac tagggacagg attggtgaca gaaaagcccc
     7201 atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca cctcctgtta
     7261 ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc tccttgccag
     7321 aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag agcttggcag
     7381 ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc accctgcgct
     7441 accctctccc agaacctgag ctgctctgac gcggctgtct ggtgcgtttc actgatcctg
     7501 gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac aaaatcagaa
     7561 taagttggtc ctgagttcta actttggctc ttcacctttc tagtccccaa tttatattgt
     7621 tcctccgtgc gtcagtttta cctgtgagat aaggccagta gccagccccg tcctggcagg
     7681 gctgtggtga ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat ggtgcgtcct
     7741 aggtgttcac caggtcgtgg ccgcctctac tccctttctc tttctccatc cttctttcct
     7801 taaagagtcc ccagtgctat ctgggacata ttcctccgcc cagagcaggg tcccgcttcc
     7861 ctaaggccct gctctgggct tctgggtttg agtccttggc aagcccagga gaggcgctca
     7921 ggcttccctg tcccccttcc tcgtccacca tctcatgccc ctggctctcc tgccccttcc
     7981 ctacaggggt tcctggctct gctctaaggg cgaattcgcg gccgctaaat tcaattcgcc
     8041 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     8101 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     8161 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat acgtacggca
     8221 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     8281 tgatattatt gacacgccgg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     8341 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     8401 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     8461 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     8521 aatgtcaggc atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc
     8581 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag
     8641 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct
     8701 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg
     8761 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg
     8821 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     8881 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     8941 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     9001 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     9061 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     9121 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     9181 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     9241 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     9301 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     9361 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     9421 cgtcgtgacc catgcatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     9481 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     9541 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     9601 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     9661 tcttctgaat tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     9721 gcggtatttc acaccgcatc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     9781 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagattatc aaaaaggatc
     9841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9901 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9961 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10021 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10081 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10141 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10201 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10261 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10321 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10381 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10441 tccgtaagat gcttttctgt gactggtgaa tactcaacca agtcattctg agaatagtgt
    10501 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10561 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10621 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10681 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10741 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10801 tgaagcattt atcagggtta ttgtctcatg accaaaatcc cttaacgtga gttttcgttc
    10861 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    10921 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    10981 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    11041 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    11101 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    11161 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    11221 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    11281 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    11341 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    11401 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    11461 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    11521 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    11581 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    11641 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    11701 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
    11761 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    11821 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    11881 aacagctatg accatgatta cgccaagctc agaattaacc ctcactaaag ggactag
//
