LOCUS       sequence-354923-       11131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor plasmid for inducible expression of CCND2 in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11131)
  AUTHORS   Joshi J, Xu B, Rubart M, Chang Y, Bao X, Chaliki HP, Scott LR, Zhu W
  TITLE     Optogenetic Control of Engrafted Human Induced Pluripotent Stem 
            Cell-Derived Cardiomyocytes in Live Mice: A Proof-of-Concept Study.
  JOURNAL   Cells. 2022 Mar 10;11(6). pii: cells11060951. doi: 
            10.3390/cells11060951.
  PUBMED    35326403
REFERENCE   2  (bases 1 to 11131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2022-03-10"; volume: "11(6). pii: cells11060951. doi"; pages:
            " 10.3390/cells11060951"
COMMENT     Sequence Label: AAVS1-Puro XLone-CCND2
FEATURES             Location/Qualifiers
     source          1..11131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(2..20)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(41..57)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    65..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(89..95,96..113,114..119))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    134..155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(272..289)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(443..1031)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(523..542)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1155..1223,1224..2015)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1373..1392)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(2376..2395)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2990..3009
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(3412..3714)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3514..3533)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     3837..3859
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3851..3868
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3852..3868
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3875..3894
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3875..3893
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3928..4764
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    4838..4859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4874..4879,4880..4897,4898..4904)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4912..4928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4936..4952
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5295..5314
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5313..5368
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5414..5433
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5543..6019
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     5801..5820
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     misc_feature    6051..6639
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6157..6168
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6566..6586
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6670..7416)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7417..7440)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7440..7449
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7469..7680)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     promoter        7722..8089
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    7730..7748
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7766..7784
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7802..7820
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7838..7856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7874..7892
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7910..7928
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7946..7964
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      8096..8105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    9152..9286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     9230..9249
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    9311..9535
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     9524..9541
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(9573..10172)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9656..9676)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     10153..10172
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(10182..10235)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    10259..10284
                     /label=SA
                     /note="splice acceptor site"
     misc_feature    10291..11094
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 ccctttagtg agggttaatt ctgagcttgg cgtaatcatg gtcatagctg tttcctgtgt
       61 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      121 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      181 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      601 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      781 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      841 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      901 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      961 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1081 actcacgtta agggattttg gtcatgagac aataaccctg ataaatgctt caataatatt
     1141 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1201 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1261 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1321 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1381 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1441 ctcagaatga cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga
     1501 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1561 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1621 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1681 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1741 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1801 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1861 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1921 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1981 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     2041 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2101 ttgataatct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2161 ttccgcgcac atttccccga aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc
     2221 gtaaggagaa aataccgcat caggaaattg taagcgttaa taattcagaa gaactcgtca
     2281 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     2341 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     2401 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     2461 ttttccacca tgatattcgg caagcaggca tcgccatgca tgggtcacga cgagatcctc
     2521 gccgtcgggc atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     2581 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     2641 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     2701 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     2761 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     2821 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     2881 ttgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     2941 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     3001 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     3061 catgcgaaac gatcctcatc ctgtctcttg atcagagctt gatcccctgc gccatcagat
     3121 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     3181 cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg
     3241 ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca
     3301 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     3361 cgtgaaaagg atctaggtga agatcctttt tgataatctc atgcctgaca tttatattcc
     3421 ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac
     3481 ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct
     3541 ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg
     3601 tgcactggcc agggggatca ccatccgtcg ccccggcgtg tcaataatat cactctgtac
     3661 atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa actgccgtac
     3721 gtataggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     3781 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     3841 gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt
     3901 gaatttagcg gccgcgaatt cgcccttaga gcagagccag gaacccctgt agggaagggg
     3961 caggagagcc aggggcatga gatggtggac gaggaagggg gacagggaag cctgagcgcc
     4021 tctcctgggc ttgccaagga ctcaaaccca gaagcccaga gcagggcctt agggaagcgg
     4081 gaccctgctc tgggcggagg aatatgtccc agatagcact ggggactctt taaggaaaga
     4141 aggatggaga aagagaaagg gagtagaggc ggccacgacc tggtgaacac ctaggacgca
     4201 ccattctcac aaagggagtt ttccacacgg acacccccct cctcaccaca gccctgccag
     4261 gacggggctg gctactggcc ttatctcaca ggtaaaactg acgcacggag gaacaatata
     4321 aattggggac tagaaaggtg aagagccaaa gttagaactc aggaccaact tattctgatt
     4381 ttgtttttcc aaactgcttc tcctcttggg aagtgtaagg aagctgcagc accaggatca
     4441 gtgaaacgca ccagacagcc gcgtcagagc agctcaggtt ctgggagagg gtagcgcagg
     4501 gtggccactg agaaccgggc aggtcacgca tccccccctt ccctcccacc ccctgccaag
     4561 ctctccctcc caggatcctc tctggctcca tcgtaagcaa accttagagg ttctggcaag
     4621 gagagagatg gctccaggaa atgggggtgt gtcaccagat aaggaatctg cctaacagga
     4681 ggtgggggtt agacccaata tcaggagact aggaaggagg aggcctaagg atggggcttt
     4741 tctgtcacca atcctgtccc tagtaaagct tagtactgtc gatccgctgg cacgacaggt
     4801 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     4861 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     4921 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt gggctgcagg
     4981 tcgagggatc tccataagag aagagggaca gctatgactg ggagtagtca ggagaggagg
     5041 aaaaatctgg ctagtaaaac atgtaaggaa aattttaggg atgttaaaga aaaaaataac
     5101 acaaaacaaa atataaaaaa aatctaacct caagtcaagg cttttctatg gaataaggaa
     5161 tggacagcag ggggctgttt catatactga tgacctcttt atagccaacc tttgttcatg
     5221 gcagccagca tatgggcata tgttgccaaa ctctaaacca aatactcatt ctgatgtttt
     5281 aaatgatttg ccctcccata tgtccttccg agtgagagac acaaaaaatt ccaacacact
     5341 attgcaatga aaataaattt cctttattag ccagaagtca gatgctcaag gggcttcatg
     5401 atgtccccat aatttttggc agagggaaaa agatctcagt ggtatttgtg agccagggca
     5461 ttggccacac cagccaccac cttctgatag gcagcctgca cctgaggagt gaattcacgc
     5521 gcctggttac ctacaaaatc agaaggacag ggaagggagc agtggttcac gcctgtaatc
     5581 ccagcaattt gggaggccaa ggtgggtaga tcacctgaga ttaggagttg gagaccagcc
     5641 tggccaatat ggtgaaaccc cgtctctacc aaaaaaacaa aaattagctg agcctggtca
     5701 tgcatgcctg gaatcccaac aactcgggag gctgaggcag gagaatcgct tgaacccagg
     5761 aggcggagat tgcagtgagc caagattgtg ccactgcact ccagcttggt tcccaataga
     5821 ccccgcaggc cctacaggtt gtcttcccaa cttgcccctt gctccatacc acccccctcc
     5881 accccataat attatagaag gacacctagt cagacaaaat gatgcaactt aattttatta
     5941 ggacaaggct ggtgggcact ggagtggcaa cttccagggc caggagaggc actggggagg
     6001 ggtcacaggg atgccacccg tagatctctc gagcagcgct cggtatcgat gcggggaggc
     6061 ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag
     6121 agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca
     6181 gaaggacgtc ccgcgcagaa tccaggtggc aacataggcg agcagccaag gaaaggacga
     6241 tgatttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca
     6301 gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt
     6361 cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa
     6421 cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag
     6481 caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata
     6541 caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc
     6601 agtcaatctt tcacaaattt tgtaatccag aggttgatta tcgataagct tgatatcgaa
     6661 ttcacgcgtt tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag
     6721 catatcaagg tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc
     6781 aagggcgtcg gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat
     6841 tagttccagg ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc
     6901 ttgtttcaga agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac
     6961 ttgatgctcc tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc
     7021 gtacagtgcg ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag
     7081 ggtttcgtac tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg
     7141 tgagaggaga gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc
     7201 gccttccagg gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc
     7261 gagcagggcc cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt
     7321 ttgagcgagt ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc
     7381 agagtttatg actttgctct tgtccagtct agacatctta tcgtcatcgt ctttgtaatc
     7441 catggtggcg gatcccgcgt cacgacacct gtgttctggc ggcaaacccg ttgcgaaaaa
     7501 gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc
     7561 ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg
     7621 ttcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc
     7681 actgacgggc accggagcca ctcgagtgga attatcacct cgagtttact ccctatcagt
     7741 gatagagaac gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta
     7801 ctccctatca gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt
     7861 atgaccagtt tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt
     7921 gatagagaac gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct
     7981 tatgtaaacc agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca
     8041 acacttttgt cttataccaa ctttccgtac cacttcctac cctcgtaaag gtaccgccac
     8101 catggagctg ctgtgccacg aggtggaccc ggtccgcagg gccgtgcggg accgcaacct
     8161 gctccgagac gaccgcgtcc tgcagaacct gctcaccatc gaggagcgct accttccgca
     8221 gtgctcctac ttcaagtgcg tgcagaagga catccaaccc tacatgcgca gaatggtggc
     8281 cacctggatg ctggaggtct gtgaggaaca gaagtgcgaa gaagaggtct tccctctggc
     8341 catgaattac ctggaccgtt tcttggctgg ggtcccgact ccgaagtccc atctgcaact
     8401 cctgggtgct gtctgcatgt tcctggcctc caaactcaaa gagaccagcc cgctgaccgc
     8461 ggagaagctg tgcatttaca ccgacaactc catcaagcct caggagctgc tggagtggga
     8521 actggtggtg ctggggaagt tgaagtggaa cctggcagct gtcactcctc atgacttcat
     8581 tgagcacatc ttgcgcaagc tgccccagca gcgggagaag ctgtctctga tccgcaagca
     8641 tgctcagacc ttcattgctc tgtgtgccac ggactttaag tttgccatgt acccaccgtc
     8701 gatgatcgca actggaagtg tgggagcagc catctgtggg ctccagcagg atgaggaagt
     8761 gagctcgctc acttgtgatg ccctgactga gctgctggct aagatcacca acacagacgt
     8821 ggattgtctc aaagcttgcc aggagcagat tgaggcggtg ctcctcaata gcctgcagca
     8881 gtaccgtcag gaccaacgtg acggatccaa gtcggaggat gaactggacc aagccagcac
     8941 ccctacagac gtgcgggata tcgacctgtg aactagtaga ccacctcccc tgcgagctaa
     9001 gctggacagc caatgacggg taagagagtg acatttttca ctaacctaag acaggagggc
     9061 cgtcagagct actgcctaat ccaaagacgg gtaaaagtga taaaaatgta tcactccaac
     9121 ctaagacagg cgcagcttcc gagggatttg agatccagac atgataagat acattgatga
     9181 gtttggacaa accaaaacta gaatgcagtg aaaaaaatgc cttatttgtg aaatttgtga
     9241 tgctattgcc ttatttgtaa ccattataag ctgcaataaa caagttctag tcaataatca
     9301 atgtcgagac ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc
     9361 tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa
     9421 tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc
     9481 aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag
     9541 gctgatcagc gggtttaaac gggccctcta gatcaggcac cgggcttgcg ggtcatgcac
     9601 caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg
     9661 tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg
     9721 gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc
     9781 gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg
     9841 ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg
     9901 atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg
     9961 gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt
    10021 tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg
    10081 gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg
    10141 gcgaggcgca ccgtgggctt gtactcggtc atctcgagcc tagggccggg attctcctcc
    10201 acgtcaccgc atgttagaag acttcctctg ccctctccgc tgccagatct ctcgaggccc
    10261 tgtgggagga agagaagagg tcagaagctt gccccactgt ggggtggagg ggacagataa
    10321 aagtacccag aaccagagcc acattaaccg gccctgggaa tataaggtgg tcccagctcg
    10381 gggacacagg atccctggag gcagcaaaca tgctgtcctg aagtggacat aggggcccgg
    10441 gttggaggaa gaagactagc tgagctctcg gacccctgga agatgccatg acagggggct
    10501 ggaagagcta gcacagacta gagaggtaag gggggtaggg gagctgccca aatgaaagga
    10561 gtgagaggtg acccgaatcc acaggagaac ggggtgtcca ggcaaagaaa gcaagaggat
    10621 ggagaggtgg ctaaagccag ggagacgggg tactttgggg ttgtccagaa aaacggtgat
    10681 gatgcaggcc tacaagaagg ggaggcggga cgcaagggag acatccgtcg gagaaggcca
    10741 tcctaagaaa cgagagatgg cacaggcccc agaaggagaa ggaaaaggga acccagcgag
    10801 tgaagacggc atggggttgg gtgagggagg agagatgccc ggagaggacc cagacacggg
    10861 gaggatccgc tcagaggaca tcacgtggtg cagcgccgag aaggaagtgc tccggaaaga
    10921 gcatccttgg gcagcaacac agcagagagc aaggggaaga gggagtggag gaagacggaa
    10981 cctgaaggag gcggcaggga aggatctggg ccagccgtag aggtgaccca ggccacaagc
    11041 tgcagacaga aagcggcaca ggcccagggg agagaatgct ggtcagagaa agcaaagggc
    11101 gaattcgttt aaacctgcag gactagctag t
//
