LOCUS       sequence-354928-       11815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor plasmid for cardiac-specific CCND2-T2A-luciferase expression 
            in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11815)
  AUTHORS   Joshi J, Xu B, Rubart M, Chang Y, Bao X, Chaliki HP, Scott LR, Zhu W
  TITLE     Optogenetic Control of Engrafted Human Induced Pluripotent Stem 
            Cell-Derived Cardiomyocytes in Live Mice: A Proof-of-Concept Study.
  JOURNAL   Cells. 2022 Mar 10;11(6). pii: cells11060951. doi: 
            10.3390/cells11060951.
  PUBMED    35326403
REFERENCE   2  (bases 1 to 11815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2022-03-10"; volume: "11(6). pii: cells11060951. doi"; pages:
            " 10.3390/cells11060951"
COMMENT     Sequence Label: AAVS1-Puro cTnT CCND2-T2A-luciferase
FEATURES             Location/Qualifiers
     source          1..11815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..836
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    843..868
                     /label=SA
                     /note="splice acceptor site"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             955..1554
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(955..974)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1451..1471
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1586..1603)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1592..1816
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2460..2478
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2510..2534
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2547..2556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3429..3482
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3483..5135
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPISKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    5172..5760
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5225..5245)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5643..5654)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5792..6268
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5991..6010)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6378..6397)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6443..6498
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6497..6516)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6859..6875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6883..6899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6907..6913,6914..6931,6932..6937))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6952..6973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    7047..7883
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(7918..7936)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7943..7960)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7943..7959)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7952..7974)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8097..8399
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8278..8297
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(8802..8821)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9416..9435
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9796..10587,10588..10656))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10419..10438
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10780..11368
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11269..11288
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11522..11539
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11656..11677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11692..11697,11698..11715,11716..11722)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11730..11746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11754..11770
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11791..11809
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagatgacc
      961 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     1021 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     1081 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     1141 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     1201 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     1261 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     1321 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     1381 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     1441 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     1501 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatctaga
     1561 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     1621 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1681 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1741 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1801 gcggtgggct ctatgggtct cgacattgat tattgactag tgtcatggag aagacccacc
     1861 ttgcagatgt cctcactggg gctggcagag ccggcaacct gcccaaggct gctcagtcca
     1921 ttaggagcca gtagcctgga agatgtcttt acccccagca tcagttcaag tggagcagca
     1981 cataactctt gccctctgcc ttccaagatt ctggtgctga gacttatgga gtgtcttgga
     2041 ggttgccttc tgccccccaa ccctgctccc agctggccct cccaggcctg ggttgctggc
     2101 ctctgcttta tcaggattct caagagggac agctggttta tgttgcatga ctgttccctg
     2161 catatctgct ctggttttaa atagcttatc tgagcagctg gaggaccaca tgggcttata
     2221 tggcgtgggg tacatgttcc tgtagccttg tccctggcac ctgccaaaat agcagccaac
     2281 accccccacc cccaccgcca tccccctgcc ccacccgtcc cctgtcgcac attcctccct
     2341 ccgcagggct ggctcaccag gccccagccc acatgcctgc ttaaagccct ctccatcctc
     2401 tgcctcaccc agtccccgct gagactgagc agacgcctcc aggatccgcc accctcgagt
     2461 aatacgactc actataggga gacccaagct ggctaggtaa gcttgatcaa caagtttgta
     2521 caaaaaagca ggctgtcgac ggtaccgcca ccatggagct gctgtgccac gaggtggacc
     2581 cggtccgcag ggccgtgcgg gaccgcaacc tgctccgaga cgaccgcgtc ctgcagaacc
     2641 tgctcaccat cgaggagcgc taccttccgc agtgctccta cttcaagtgc gtgcagaagg
     2701 acatccaacc ctacatgcgc agaatggtgg ccacctggat gctggaggtc tgtgaggaac
     2761 agaagtgcga agaagaggtc ttccctctgg ccatgaatta cctggaccgt ttcttggctg
     2821 gggtcccgac tccgaagtcc catctgcaac tcctgggtgc tgtctgcatg ttcctggcct
     2881 ccaaactcaa agagaccagc ccgctgaccg cggagaagct gtgcatttac accgacaact
     2941 ccatcaagcc tcaggagctg ctggagtggg aactggtggt gctggggaag ttgaagtgga
     3001 acctggcagc tgtcactcct catgacttca ttgagcacat cttgcgcaag ctgccccagc
     3061 agcgggagaa gctgtctctg atccgcaagc atgctcagac cttcattgct ctgtgtgcca
     3121 cggactttaa gtttgccatg tacccaccgt cgatgatcgc aactggaagt gtgggagcag
     3181 ccatctgtgg gctccagcag gatgaggaag tgagctcgct cacttgtgat gccctgactg
     3241 agctgctggc taagatcacc aacacagacg tggattgtct caaagcttgc caggagcaga
     3301 ttgaggcggt gctcctcaat agcctgcagc agtaccgtca ggaccaacgt gacggatcca
     3361 agtcggagga tgaactggac caagccagca cccctacaga cgtgcgggat atcgacctgg
     3421 gcagcggaga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
     3481 ctatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg
     3541 ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca
     3601 tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga
     3661 gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg
     3721 tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg
     3781 tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca
     3841 tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc
     3901 aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg
     3961 gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg
     4021 acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg
     4081 gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca
     4141 gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg
     4201 tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct
     4261 ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact
     4321 ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc
     4381 tcatcgacaa gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgatca
     4441 gcaaggaggt aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct
     4501 acggcctgac agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg
     4561 gcgcagtagg caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta
     4621 agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg
     4681 gctacgttaa caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca
     4741 gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac cggctgaaga
     4801 gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc atcctgctgc
     4861 aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat gccggcgagc
     4921 tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg
     4981 actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg
     5041 acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc gagattctca
     5101 ttaaggccaa gaagggcggc aagatcgccg tgtaaactag tacgcgtgaa ttcgatatca
     5161 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5221 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5281 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5341 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5401 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5461 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5521 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     5581 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5641 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5701 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5761 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     5821 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     5881 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     5941 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     6001 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     6061 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     6121 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     6181 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     6241 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccaggc gcgtgaattc
     6301 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
     6361 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     6421 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     6481 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     6541 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
     6601 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     6661 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     6721 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     6781 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc
     6841 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6901 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6961 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7021 ccagcggatc gacagtacta agctttacta gggacaggat tggtgacaga aaagccccat
     7081 ccttaggcct cctccttcct agtctcctga tattgggtct aacccccacc tcctgttagg
     7141 cagattcctt atctggtgac acacccccat ttcctggagc catctctctc cttgccagaa
     7201 cctctaaggt ttgcttacga tggagccaga gaggatcctg ggagggagag cttggcaggg
     7261 ggtgggaggg aaggggggga tgcgtgacct gcccggttct cagtggccac cctgcgctac
     7321 cctctcccag aacctgagct gctctgacgc ggctgtctgg tgcgtttcac tgatcctggt
     7381 gctgcagctt ccttacactt cccaagagga gaagcagttt ggaaaaacaa aatcagaata
     7441 agttggtcct gagttctaac tttggctctt cacctttcta gtccccaatt tatattgttc
     7501 ctccgtgcgt cagttttacc tgtgagataa ggccagtagc cagccccgtc ctggcagggc
     7561 tgtggtgagg aggggggtgt ccgtgtggaa aactcccttt gtgagaatgg tgcgtcctag
     7621 gtgttcacca ggtcgtggcc gcctctactc cctttctctt tctccatcct tctttcctta
     7681 aagagtcccc agtgctatct gggacatatt cctccgccca gagcagggtc ccgcttccct
     7741 aaggccctgc tctgggcttc tgggtttgag tccttggcaa gcccaggaga ggcgctcagg
     7801 cttccctgtc ccccttcctc gtccaccatc tcatgcccct ggctctcctg ccccttccct
     7861 acaggggttc ctggctctgc tctaagggcg aattcgcggc cgctaaattc aattcgccct
     7921 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     7981 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     8041 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctatac gtacggcagt
     8101 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     8161 atattattga cacgccgggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     8221 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     8281 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     8341 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     8401 tgtcaggcat gagattatca aaaaggatct tcacctagat ccttttcacg tagaaagcca
     8461 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg
     8521 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag
     8581 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta
     8641 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
     8701 gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     8761 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     8821 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     8881 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     8941 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     9001 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     9061 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     9121 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     9181 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     9241 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     9301 tcgtgaccca tgcatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     9361 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     9421 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     9481 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     9541 ttctgaatta ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc
     9601 ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9661 tttatttttc taaatacatt caaatatgta tccgctcatg agattatcaa aaaggatctt
     9721 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9781 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9841 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9901 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9961 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10021 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10081 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10141 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10201 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10261 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10321 cgtaagatgc ttttctgtga ctggtgaata ctcaaccaag tcattctgag aatagtgtat
    10381 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10441 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10501 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10561 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10621 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10681 aagcatttat cagggttatt gtctcatgac caaaatccct taacgtgagt tttcgttcca
    10741 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    10801 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    10861 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    10921 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    10981 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    11041 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    11101 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    11161 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    11221 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    11281 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    11341 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    11401 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    11461 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    11521 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    11581 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    11641 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    11701 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    11761 cagctatgac catgattacg ccaagctcag aattaaccct cactaaaggg actag
//
